Article
Chemistry, Multidisciplinary
Gokhan Bahcecioglu, Xiaoshan Yue, Erin Howe, Ian Guldner, M. Sharon Stack, Harikrishna Nakshatri, Siyuan Zhang, Pinar Zorlutuna
Summary: The study reveals that aging ECM can drive normal mammary epithelial cells to a more invasive and cancer-like phenotype, and enhance motility and invasiveness in breast cancer cells. Different responses of cells to young and aged ECMs may lead to the identification of new targets for cancer treatment and prevention.
Article
Oncology
Sirui Long, Jie Wang, Fanbin Weng, Debing Xiang, Guiyin Sun
Summary: ECM1 is overexpressed in colorectal cancer (CRC) tissues and cell lines, and its upregulation is correlated with tumor size, lymph node status, and TNM stage in CRC patients. Knocking down ECM1 inhibits CRC cell growth, migration, and invasion, while its overexpression yields opposite effects. ECM1 overexpression also promotes the expression of p-AKT, p-GSK3 beta, and Snail. Treatment with LY294002 and 740 Y-P can reverse the impact of ECM1 upregulation and downregulation on CRC cell metastasis and EMT induction. In vivo analysis confirms that ECM1 overexpression enhances EMT induction and CRC tumor progression.
FRONTIERS IN ONCOLOGY
(2022)
Article
Environmental Sciences
Ruijia Zhang, Jin Sun, Lanyin Tu, Wenhua Lu, Yizheng Li, Tiangang Luan, Baowei Chen
Summary: Tumor cell migration induced by arsenite (iAsIII) is associated with cancer progression. In human colorectal cancer (DLD-1) cells exposed to iAsIII, transcriptomics and metabolomics approaches reveal the interactive networks of functional genes and metabolites. The results show that iAsIII treatment leads to increased cell migration, activation of epithelial to mesenchymal transition (EMT), enhanced cell adhesion, matrix degradation through matrix metallopeptidases (MMPs), and interference with glutamine metabolism.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Biotechnology & Applied Microbiology
Long Yang, Jingwen Li, Guangchao Zang, Sijie Song, Zhengwen Sun, Xinyue Li, Yuanzhu Li, Zhenhong Xie, Guangyuan Zhang, Ni Gui, Shu Zhu, Tingting Chen, Yikui Cai, Yinping Zhao
Summary: High matrix stiffness promotes EMT and migration of cervical cancer cells through the Pin1/YAP pathway.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Pathology
Yue Yang, Ting Yan, Qiang Han, Meiyu Zhang, Yijun Zhang, Yuan Luo, Lai Wei, Pengcheng Li, Enhua Wang
Summary: ZNF326 expression was higher in colorectal tumor cells compared to normal mucosa, and its high expression was positively correlated with tumor differentiation, TNM staging, and lymph node metastasis. ZNF326 overexpression promoted colorectal cancer cell invasion and altered the expression of EMT-related proteins. Upregulation of ZNF326 also increased LTBP4 and p-Smad2/3 expression, leading to TGF-beta signaling activation.
PATHOLOGY RESEARCH AND PRACTICE
(2021)
Article
Cell Biology
Xinyue Yu, Qian Wang, Baocai Liu, Ning Zhang, Guanghui Cheng
Summary: Vitamin D3 demonstrates antitumor effects on colorectal cancer cells and sensitizes them to ionizing radiation by regulating apoptosis, autophagy, and epithelial-mesenchymal transition (EMT). Proteomics analysis reveals that VD3 can modulate cell migration and cadherin expression to enhance radiosensitivity. Additionally, VD3 can partially block the JAK/STAT3 signaling pathway to increase sensitivity to radiation, providing a novel insight for improving therapeutic efficacy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Ruihua Jing, Conghui Hu, Tiantian Qi, Jiaqi Yue, Guangyan Wang, Ming Zhang, Chan Wen, Cheng Pei, Bo Ma
Summary: The study revealed that TGF-β2 induced cell apoptosis, which could contribute to EMT and ECM synthesis during PCO. Inhibition of apoptosis-associated protein ZVAD decreased expression of EMT and ECM markers. RNA-seq analysis identified FILIP1L as a key factor in regulating cell migration and EMT/ECM synthesis through apoptosis in PCO. In conclusion, apoptosis mediated by FILIP1L plays a significant role in the development of PCO and can serve as a potential drug target for future treatments.
Article
Oncology
Jing Zhang, Zhimin Hu, Calista A. Horta, Jing Yang
Summary: Epithelial-mesenchymal transition (EMT) is involved in various aspects of tumor development. The tumor microenvironment, consisting of different stroma cells and biochemical factors, influences the EMT program and tumor progression. This review explores the effects of tumor microenvironmental signals on EMT regulation, discusses the molecular mechanisms of EMT in therapy resistance, and proposes new therapeutic approaches targeting the tumor microenvironment to impact EMT and tumor progression.
SEMINARS IN CANCER BIOLOGY
(2023)
Article
Cell Biology
Rahul Bhome, Muhammad Emaduddin, Victoria James, Louise M. House, Stephen M. Thirdborough, Massimiliano Mellone, Joeri Tulkens, John N. Primrose, Gareth J. Thomas, Olivier De Wever, Alex H. Mirnezami, A. Emre Sayan
Summary: This study reveals a new mechanism of cell communication in CRC through the EV-mediated transfer of miRNAs, explaining why CRC tumors with greater metastatic potential are rich in CAFs.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Biochemistry & Molecular Biology
Yanwen Mao, Xiaohuan Zhang, Wei Peng, Huiming Liu, Xingchen Zhou, Luqun Liang, Jiayi Xiang, Huifang Zhang, Dan Wang, Lingling Liu, Yuxia Zhou, Fan Zhang, Ying Xiao, Mingjun Shi, Yuanyuan Wang, Bing Guo
Summary: EI24 plays a tumor suppressor role in kidney diseases by inhibiting renal interstitial fibrosis through inhibiting epithelial-mesenchymal transition and excessive extracellular matrix deposition, as well as reducing inflammation by restraining NF-kappa B and JNK pathways.
Article
Biochemistry & Molecular Biology
Patricia F. de Souza Oliveira, Alessandra V. S. Faria, Stefano P. Clerici, Erica M. Akagi, Hernandes F. Carvalho, Giselle Z. Justo, Nelson Duran, Carmen Ferreira-Halder
Summary: This study evaluates the ability of violacein to interfere with cancer hallmarks and demonstrates its inhibitory effects on cell migration and signaling pathways in colorectal cancer cells and spheroids. These findings further reinforce the potential of violacein as an antitumor agent.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Huihai Yang, Grace Gar-Lee Yue, Ping-Chung Leung, Chun-Kwok Wong, Ying-Jun Zhang, Clara Bik-San Lau
Summary: This study found that the natural gallotannin PGG has anti-tumor effects on colon cancer cells and inhibits cell proliferation, colony formation, motility, and migration. It also demonstrated that PGG suppresses liver and lung metastasis in mouse models and regulates immune cell populations and cytokine levels. These findings suggest that PGG has the potential to be developed as an anti-metastatic agent for metastatic CRC.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Chemistry, Medicinal
Syed Afroz Ali, Mohd Aslam Saifi, Chandraiah Godugu, Venu Talla
Summary: The study demonstrated the anti-fibrotic activity of silibinin in pulmonary fibrosis, alleviating inflammatory response and modulating epithelial-mesenchymal phenotypic alterations.
PHYTOTHERAPY RESEARCH
(2021)
Article
Oncology
Pelin Balcik-Ercin, Laure Cayrefourcq, Rama Soundararajan, Sendurai A. Mani, Catherine Alix-Panabieres
Summary: Metastasis is a complex and not fully understood process in cancer, where circulating tumor cells (CTCs) play a crucial role. CTCs possess a unique ability to transition between epithelial and mesenchymal states, known as epithelial-to-mesenchymal plasticity (EMP), which aids in their metastatic potential. This study focuses on the EMP phenotype of colon CTC lines, showing that these cells have minimal mesenchymal features but exhibit an increase in mesenchymal-epithelial transition (MET) markers, suggesting a quick reversion to the epithelial state for successful metastasis.
Article
Multidisciplinary Sciences
Boyang Fan, Chaojing Zheng, Ning Wang, Zewen Chang, Yunxiao Liu, Chunlin Wang, Jun Xiang, Yangbao Tao, Guiyu Wang, Qian Zhang
Summary: The study identifies a novel circRNA, circSTK3, which is significantly upregulated in colorectal cancer (CRC) tissues and is associated with decreased survival. Functional assays show that circSTK3 promotes cell migration and invasion, and gene expression analysis reveals its involvement in CRC metastasis. The study also uncovers that CTCF transcriptionally regulates circSTK3. These findings highlight the functional and prognostic roles of circSTK3 and demonstrate the importance of circRNAs in metastasis.
Article
Respiratory System
Hannelore P. Van Eeckhoutte, Chantal Donovan, Richard Y. Kim, Thomas M. Conlon, Meshal Ansari, Haroon Khan, Ranjith Jayaraman, Nicole G. Hansbro, Yves Dondelinger, Tom Delanghe, Allison M. Beal, Brad Geddes, John Bertin, Tom Vanden Berghe, Joyceline De Volder, Tania Maes, Peter Vandenabeele, Bart M. Vanaudenaerde, Dieter Deforce, Sonja Skevin, Filip Van Nieuwerburgh, Fien M. Verhamme, Guy F. Joos, Sobia Idrees, Herbert B. Schiller, Ali Oender Yildirim, Alen Faiz, Mathieu J. M. Bertrand', Guy G. Brusselle, Philip M. Hansbro, Ken R. Bracke
Summary: RIPK1 plays a key role in COPD pathogenesis by mediating cell death and inflammation. Inhibition of RIPK1 kinase activity can alleviate airway inflammation and cell death caused by COPD, making it a potential therapeutic approach.
EUROPEAN RESPIRATORY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Kurtis F. Budden, Shaan L. Gellatly, Annalicia Vaughan, Nadia Amorim, Jay C. Horvat, Nicole G. Hansbro, David L. A. Wood, Philip Hugenholtz, Paul G. Dennis, Peter A. B. Wark, Philip M. Hansbro
Summary: In this study, it was found that regardless of its acetate-producing capacity, Bifidobacterium longum subsp. longum alleviated cigarette smoke-induced inflammation, reduced inflammatory cytokine expression and adhesion factor expression, and improved the depletion of cecal butyrate levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Respiratory System
Venkata Sita Rama Raju Allam, Stelios Pavlidis, Gang Liu, Nazanin Zounemat Kermani, Jennifer Simpson, Joyce To, Sheila Donnelly, Yi-Ke Guo, Philip M. Hansbro, Simon Phipps, Eric F. Morand, Ratko Djukanovic, Peter Sterk, Kian Fan Chung, Ian Adcock, James Harris, Maria B. Sukkar
Summary: The study showed that MIF protein correlates with clinical and molecular features of severe neutrophilic asthma and reduces the biological activity of annexin-A1 through interaction, promoting glucocorticoid resistance of neutrophilic inflammation.
Article
Pharmacology & Pharmacy
Vamshikrishna Malyla, Gabriele De Rubis, Keshav Raj Paudel, Dinesh Kumar Chellappan, Nicole G. Hansbro, Philip M. Hansbro, Kamal Dua
Summary: Lung cancer is the leading cause of cancer-related deaths globally, with over 1.9 million cases each year. Inhibiting the beta-catenin pathway can attenuate the progression of lung cancer. Encapsulating berberine into liquid crystalline nanoparticles improves its physiochemical functions and targets the beta-catenin pathway to inhibit human lung adenocarcinoma cells at both gene and protein levels. This represents a promising strategy for managing lung cancer progression.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Article
Biochemical Research Methods
Yajie Tang, Shengming Ma, Sen Lin, Yinrong Wu, Siyang Chen, Gang Liu, Lisong Ma, Zaihua Wang, Lele Jiang, Yao Wang
Summary: In this study, high-quality soluble CD1E was successfully synthesized using an E.coli cell-free protein synthesis system, and it was purified with >= 90% purity by Ni2+ affinity chromatography. The protein interaction network of CD1E was predicted using the string website, and a potential binding partner B2M was identified. The interaction between CD1E and B2M was verified using Surface Plasmon Resonance (SPR).
PROTEIN EXPRESSION AND PURIFICATION
(2023)
Editorial Material
Respiratory System
Philip Hansbro
Article
Cardiac & Cardiovascular Systems
Jiachen Qi, Weidong Yan, Gang Liu, Yuan Teng, Sizhe Gao, Shujie Yan, Jian Wang, Boyi Zhou, Bingyang Ji
Summary: This study aimed to evaluate the incidence of acute kidney injury (AKI) defined by the Kidney Disease: Improving Global Outcomes (KDIGO) group in patients supported by veno-arterial extracorporeal membrane oxygenation (VA ECMO) after post-cardiotomy cardiogenic shock (PCS), and to identify the risk factors for AKI >3. The results showed that the incidence of AKI >3 in patients supported by VA ECMO after PCS was 58.8%. Patients with AKI >3 required significantly longer mechanical ventilation and hospital stay. Intraoperative implantation of VA ECMO was associated with a decreased incidence of AKI >3.
REVIEWS IN CARDIOVASCULAR MEDICINE
(2023)
Review
Chemistry, Multidisciplinary
Gang Liu, Ziyu Lv, Saima Batool, Ming-Zheng Li, Pengfei Zhao, Liangchao Guo, Yan Wang, Ye Zhou, Su-Ting Han
Summary: With the rapid growth of artificial intelligence, big data, the Internet of Things, and 5G/6G technologies, there is a growing need for humans to pursue life and manage personal or family health. The use of micro biosensing devices is crucial in bridging the gap between technology and personalized medicine.
Retraction
Chemistry, Multidisciplinary
Gang Liu, Yun Huang, Kunqiu Yang, Zhen Cao, Zhanwei Zhao, Chao Wang
ARABIAN JOURNAL OF CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Bikash Manandhar, Keshav Raj Paudel, Dvya Delilaa Clarence, Gabriele De Rubis, Thiagarajan Madheswaran, Jithendra Panneerselvam, Flavia C. Zacconi, Kylie A. Williams, Lisa G. Pont, Majid Ebrahimi Warkiani, Ronan MacLoughlin, Brian Gregory Oliver, Gaurav Gupta, Sachin Kumar Singh, Dinesh Kumar Chellappan, Philip M. Hansbro, Kamal Dua
Summary: Lung cancer is a common and deadly form of cancer, especially non-small-cell lung cancer (NSCLC). Zerumbone (ZER) is a natural compound with anti-cancer properties, but its effectiveness is hindered by low solubility and poor absorption. This study developed ZER-loaded liquid crystalline nanoparticles (LCNs) and found that they effectively inhibited the growth and migration of NSCLC cells. The underlying mechanisms involved the regulation of tumor suppressor genes and metastasis-associated genes. This research suggests that ZER-LCNs could be a promising treatment strategy for NSCLC.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Letter
Biology
Juman Mohammed Rasmi Alamil, Dikaia Xenaki, Bikash Manandhar, Keshav Raj Paudel, Philip Michael Hansbro, Brian Gregory Oliver, Dinesh Kumar Chellappan, Kamal Dua
Letter
Biology
Keshav Raj Paudel, Bikash Manandhar, Sachin Kumar Singh, Gaurav Gupta, Philip Michael Hansbro, Dinesh Kumar Chellappan, Kamal Dua
Meeting Abstract
Respiratory System
Olivia Carroll, Alexandra Brown, Jemma Mayall, Henry Gomez, Richard Kim, Chantal Donovan, Evan Williams, Bronwyn Berthon, James Pinkerton, Philip Hansbro, Paul Foster, Katie Wynne, Hayley Scott, Lisa Wood, Jay Horvat
Letter
Biology
Mohamad Siddiq Bin Mohamad, Ruby-Jean Reyes, Gabriele De Rubis, Keshav Raj Paudel, Philip Michael Hansbro, Kamal Dua, Dinesh Kumar Chellappan
Article
Respiratory System
Xiaofan Tu, Henry M. Gomez, Richard Y. Kim, Alexandra C. Brown, Emma de Jong, Izabela Galvao, Alen Faiz, Anthony Bosco, Jay C. Horvat, Philip Hansbro, Chantal Donovan
Summary: Lung transcriptomics studies have provided valuable information in asthma research, but it is important to understand the individual contributions of the airway and parenchyma in disease development. This study used a house dust mite-induced asthma model to analyze the transcriptomics of the airway and parenchyma. The results identified several transcription factors that regulate gene expression in these compartments, and further investigation showed that inhibiting the lung SPI1 response had positive effects on key disease outcomes. This study highlights the importance of SPI1 in experimental asthma and suggests its potential as a target for treatment.
RESPIRATORY RESEARCH
(2023)