Article
Chemistry, Analytical
Hemeiling Cai, Jing Sun, Zhichang Ma, Junwei Di
Summary: An enhanced charge transfer between BiVO4 and Au nanoparticles was utilized to develop a turn-on photoelectrochemical biosensor for sensitive detection of target DNA. The biosensor showed a linear range from 1 aM to 10 pM with a detection limit of 0.2 aM, making it a promising platform for ultrasensitive DNA detection.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Ivan Piovarci, Sopio Melikishvili, Marek Tatarko, Tibor Hianik, Michael Thompson
Summary: The study presents two methods for monitoring protease activity: one using a thickness-shear mode (TSM) sensor and the other utilizing a colorimetric assay with gold nanoparticles. Both methods demonstrate sensitivity and accuracy in detecting protease activity.
Article
Materials Science, Ceramics
Lei Zhong, Chuanjian Wu, Zhong Yu, Fangyuan Zhang, Ziyu Li, Wei Zhao, Xiaona Jiang, Lingfeng Li, Zhongwen Lan, Ke Sun
Summary: By constructing magnetoelastic stress, strontium ferrites with enhanced magnetic properties can be sintered at low temperatures. Applying suitable external pressure can further improve the magnetic and microwave properties. However, excessive pressure results in increased grain size and porosity, limiting the improvement of magnetic properties.
JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Almudena Marti, Jurriaan Huskens
Summary: Affinity sensing of nucleic acids is a highly investigated area in biosensing, and in this study, a simple electrochemical DNA detection layer is developed using poly-l-lysine and gold nanoparticles as a signal amplifier. The method shows high contrast and sensitivity, making it potentially useful in DNA diagnostics.
Article
Physics, Multidisciplinary
Lucile Soumah, Davide Bossini, Abdelmadjid Anane, Stefano Bonetti
Summary: In this study, ultrafast pump-probe measurements were performed on a nanometer-thick Bi-doped yttrium iron garnet film, revealing that optically induced excitation can increase the ferromagnetic resonance frequency of the material by up to 20%. This unexpected result is explained by a modification of the magnetic anisotropy caused by long-lived photo-induced strain that transiently and reversibly modifies the magnetoelastic coupling in the material.
PHYSICAL REVIEW LETTERS
(2021)
Article
Chemistry, Multidisciplinary
Xinru Huang, Shengbo Sang, Zhongyun Yuan, Qianqian Duan, Xing Guo, Hongpeng Zhang, Chun Zhao
Summary: Lysozymes in urine are important indicators for renal diseases. Current detection methods like ELISA are tedious, while newer methods are time-consuming and complex. A rapid, low-cost, and easy-to-prepare lysozyme detection method is still needed for clinical monitoring.
Article
Chemistry, Analytical
Yana Chen, Shuangling Wang, Jujie Ren, Haiyan Zhao, Min Cui, Na Li, Meng Li, Cong Zhang
Summary: In this study, a signal on-off ratiometric electrochemical immunosensor was developed for ultrasensitive detection of A beta oligomer, which serves as a reliable biomarker for early diagnosis of Alzheimer's disease. The sandwich-like electrochemical immunosensor exhibited high sensitivity and low detection limit, and was able to determine the content of A beta oligomer in real cerebrospinal fluid samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Priyanka R. Ipte, Sudipa Manna, Ashis Kumar Satpati
Summary: 6-Mercaptopurine (6-MP) is an anti-cancer drug used for acute lymphocytic leukaemia treatment. However, the electrochemical signal of 6-MP on unmodified Glassy carbon electrode (GCE) is weak. To enhance the signal, gold nano particles (AuNPs) were electrodeposited on the GCE. The addition of carbon nano dots (CD) in the solution further increased the peak current of 6-MP, indicating a strong interaction between 6-MP and CD. This interaction was confirmed using UV-vis and fluorescence spectroscopy. Atomic force microscopy (AFM) showed changes in dsDNA texture upon interaction with 6-MP.
MICROCHEMICAL JOURNAL
(2023)
Article
Engineering, Environmental
Zhiwei Sun, Juan Li, Yao Tong, Yufei Yang, Hui Li, Chuanxin Wang, Lutao Du, Yanyan Jiang
Summary: This article presents a ratiometric fluorescent biosensing strategy for the precise detection of colorectal cancer-associated miR-92a-3p. The strategy combines a nanoplatform composed of DNA-templated Ag-Au nanoclusters and Au nanoparticles/g-C3N4 nanosheets with catalytic hairpin assembly and sequence-induced fluorescence enhancement. It demonstrates a low detection limit, excellent selectivity, and the ability to detect miR-92a-3p in clinical exosome samples.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Nanoscience & Nanotechnology
Yuanyuan Zhang, Jinwen Zhao, Guomin Yang, Ying He, Shihong Chen, Ruo Yuan
Summary: A quadrivalent cruciform DNA nanostructure (qCDN)-mediated cascaded catalyzed hairpin assembly (CHA) amplifier was developed for the fluorescence detection of amyloid beta oligomer species (A beta Os), achieving high FRET efficiency and fast reaction rate, with a detection limit as low as 0.69 pM. The combination of DD-A FRET binary probes and qCDN-mediated cascaded amplifier shows great promise for detecting biomarkers at trace levels.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Engineering, Electrical & Electronic
Santosh Kumar, Zhu Guo, Ragini Singh, Qinglin Wang, Bingyuan Zhang, Shuang Cheng, Feng-Zhen Liu, Carlos Marques, Brajesh Kumar Kaushik, Rajan Jha
Summary: This study introduces a fiber-optic localized surface plasmon resonance (LSPR) based biosensor for sensitive detection of Shigella bacterial species. By controlled etching and coating with nanomaterials, the sensor achieves increased refractive index sensitivity and excitation of localized plasmons. Results show successful and efficient detection of Shigella with high sensitivity, making it potentially valuable in the future as an alternative to existing commercial methods.
JOURNAL OF LIGHTWAVE TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Quanjing Zhu, Haoyang Yang, Jing Luo, Hui Huang, Lichao Fang, Jun Deng, Chenghong Li, Yan Li, Tao Zeng, Junsong Zheng
Summary: A novel 3D matrixed DNA self-nanocatalyzer based on gold nanoparticles was proposed for the ultrasensitive bioassay of DNA 5-methylcytosine, showing potential applications in clinical diagnosis and disease mechanism investigation.
ANALYTICA CHIMICA ACTA
(2021)
Article
Engineering, Electrical & Electronic
Junxi Zhang, Taiming Liang, Haoyu Wang, Zhulin Huang, Zhijia Hu, Kang Xie, Wei Zhang, Yongqing Wei, Guiqiang Wang, Jian Yan, Fei Wang, Haiming Jiang, Lin Zhang
Summary: This paper demonstrates a tilted fiber grating based localized surface plasmon resonance (LSPR) biosensor for ultrasensitive glucose detection. The biosensor has high sensitivity and specificity, and has practical utility in detecting glucose in artificial urine.
IEEE SENSORS JOURNAL
(2022)
Article
Chemistry, Analytical
Xiaolu Xiong, Peng Zhu, Shanshan Li, Yujiu Jiang, Yurong Ma, Qingfan Shi, Xu Zhang, Xiaoming Shu, Zhiwei Wang, Linfeng Sun, Junfeng Han
Summary: A study explores the feasibility of using a flexible Bi2Se3 tape electrode (BTE) as an efficient bioplatform for HIV-1 DNA detection by loading Au nanoparticles. The resulting biosensor shows outstanding performance with a wide linear range, low detection limit, excellent selectivity, reproducibility, and stability, outperforming other hybrid biosensors. This is attributed to the intrinsic surface state of Bi2Se3 topological insulator in facilitating electron transfer.
Article
Chemistry, Multidisciplinary
Chen Chen, Jin-Xin Ma, Hui Wang, Hui-Qiao Liu, Shu-Wei Ren, Jun-Tao Cao, Yan-Ming Liu
Summary: A spatially resolved ratiometric electrochemiluminescence immunosensor was developed for myoglobin detection, using resonance energy transfer for signal amplification. The sensor exhibited high selectivity, sensitivity, and accuracy, making it a promising tool for biomarker analysis.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Yiran Qu, Innocent Bekard, Ben Hunt, Jamie Black, Louis Fabri, Sally L. Gras, Sandra. E. Kentish
Summary: This study compares the performance of a nanofiber device and a resin column for antibody capture. The nanofiber device has a larger housing volume and lower binding capacity, but comparable eluate purity to the resin column. It shows high stability, can be used for multiple cycles, and maintains consistent eluate quality when scaled up. The use of a single nanofiber device can significantly reduce costs compared to a resin column, especially when the number of batches is limited.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Brandon Moore, Christos Georgakis, Chris Antoniou, Sarwat Khattak
Summary: Fed-batch cell culture processes are commonly used in biomanufacturing due to their simplicity and applicability in cGMP environments. However, the challenge lies in the changing physiochemical conditions within the bioreactor as the cell density changes. Traditional response surface models (RSMs) are commonly used for optimization but are limited by their use of time-invariant factors. Dynamic RSM (DRSM) models can predict the time-dependent impact of process inputs, allowing for optimization of process operations that change over time.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lin Li, Yunfan Bai, Chuhua Qi, Yile Du, Xiaoxiao Ma, Yutong Li, Pingping Wu, Shuangli Chen, Sijing Zhang
Summary: A succinic anhydride-modified apple pomace (SAMAP) was synthesized to address environmental issues caused by the accumulation of apple pomace and effectively treat heavy metal ions. SAMAP exhibited high adsorption capacity for Cu(II) and Pb(II), suggesting its potential application in wastewater treatment.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Peter Satzer
Summary: Water for injection (WFI) production in the biopharmaceutical industry consumes excessive amounts of water and energy. Recycling buffers can potentially save up to 90% of resources, but achieving the full theoretical potential is impossible when a risk-aware design is used. Universal risk-based assessment is important for regulatory authorities to consider the implementation of such a strategy.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Gaoya Sun, Lingkai Jin, Guangxue Wang, Xiaoge Wang, Jin Huang
Summary: In this study, heterologous expression and homologous overexpression of ABC transporter proteins AatA and MdlB were found to improve butyric acid production in C. tyrobutyricum. The overexpression of these proteins upregulated the expression levels of key enzymes in the acetate synthesis pathway and promoted the synthesis and secretion of acetic acid. Additionally, the increase in ATPase activity facilitated sugar utilization, induced extracellular secretion of acetate, and shortened fermentation periods.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Taiki Okamura, Rina Aritomi, Takuya Matsumoto, Ryosuke Yamada, Hidehiko Hirakawa, Hiroyasu Ogino
Summary: In this study, proline was introduced to improve the stability of putidaredoxin reductase (PdR) in the Pseudomonas putida cytochrome P450 system. It was found that PdR_T221P had a longer half-life at high temperatures compared to wild-type PdR, but a shorter half-life in the presence of methanol. Molecular dynamics simulations supported these findings.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yuying Hu, Xiaofan Wang, Shihao Zhang, Zimu Liu, Tengfang Hu, Xin Wang, Xiaoming Peng, Hongling Dai, Jing Wu, Fengping Hu
Summary: This study investigates the effect of iron-carbon micro-electrolysis (ICME) materials on high-solid anaerobic digestion (HSAD). The results show that ICME materials promote methane production in HSAD by increasing the attachment area of microorganisms and facilitating symbiotic metabolism of certain bacterial species. This study provides new insights into microbial mechanisms and enhances our understanding of ICME material enhancement in HSAD.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jibao Liu, Yufeng Xu, Yuansong Wei
Summary: This study investigated the role of sludge rheology in anaerobic digestion (AD) and found that rheological properties increased with the increase of solid content, resulting in a negative effect on methane production. An extended ADM1 model revealed that enhanced sludge rheological properties increased mass diffusion resistance and reduced uptake rate of acetate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Xiaoyan Liu, Zongze Chen, Dewen Kong, Xinying Zhang, Chuanhua Wang, Yongqi Wang
Summary: This study explored the role of intracellular and extracellular enzymes of Acinetobacter baumannii and Talaromyces sp. in the degradation of crude oil. The extracellular enzymes of Talaromyces sp. were more effective in degrading n-alkanes, while those of Acinetobacter baumannii had a better effect on aromatic hydrocarbons. The degradation enzyme systems of both bacteria and fungi complemented each other, improving the overall degradation ability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jing Dong, Lingli Xu, Yuxiang Liu, Li Ren, Ke Yuan
Summary: The utilization of biochar-immobilized microorganisms is an effective method for eliminating phenol from water. The high susceptibility of bacteria to environmental factors is a challenge for practical implementation. In this study, biochar was used to reduce microbial susceptibility and enhance phenol removal. The addition of biochar altered the dominant species of phenol-degrading bacteria and response surface analysis indicated the significant influence of biochar pyrolysis temperature and experimental temperature on phenol removal rate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Zibin Pan, Mengying Liu, Zuliang Chen
Summary: This study successfully removed metalloids and heavy metals from acid mine drainage (AMD) using bio-synthesized Fe/Cu nanoparticles (Fe/Cu NPs). The Fe/Cu NPs showed high removal capacities and the presence of organic substances contributed to their stability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Guangbing Liu, Han Zhang, Jincan Huang, Lu Zhang, Teng Zhang, Xuemin Yu, Weijing Liu, Chunkai Huang
Summary: This study investigated the effect of Fenton pre-treatment on the treatment efficiency of printing and dyeing wastewater (PDW) using two anaerobic/aerobic-membrane bioreactors (A/O-MBRs). The results showed that Fenton pre-treatment significantly improved the removal efficiency of COD and AOX in PDW, and reduced membrane fouling. The Shannon indices and metagenomics analysis indicated that the microbial diversity in anaerobic flocs was higher than that in aerobic flocs, and EC3.1.1.45 and pcaI were identified as key functional genes.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lidia Borgosz, Duygu Dikicioglu
Summary: The Industrial Internet of Things (IIoT) is a system that connects devices and provides real-time insight into industrial processes. However, the complexity and regulatory requirements of the biomanufacturing sector make it challenging to implement IIoT. There is a need for universal solutions to overcome this challenge and advance the field of biomanufacturing.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yajie Li, Weikang Kong, Yuyao Zhang, Huarui Zhou, Hongbo Liu, Salma Tabassum
Summary: In this study, the iron-carbon multi-micro electric field coupling anaerobic co-digestion technique was used to treat coal gasification wastewater (CGW). The experimental results showed that under optimal operating conditions, this technique can significantly reduce the toxicity of the wastewater and achieve high removal efficiencies. Additionally, the analysis of microbial communities revealed that the coupling system promotes direct interspecies electron transfer.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Aparecido Nivaldo Modenes, Debora Gozzi Fernandes, Daniela Estelita Goes Trigueros, Matheus Guilherme Amador, Fernando Rodolfo Espinoza-Quinones, Taysa de Souza Braniz, Adilson Ricken Schuelter, Glacy Jaqueline da Silva, Lucimar Pereira Bonett
Summary: This study aimed to systematically remove organic pollutants from raw dairy wastewater with high concentrations of COD, TOC, and TN using Poterioochromonas malhamensis algae strains. The results showed that the biomass yield rate using FP-PBRs was 10% higher than tubular PBRs, and the organic pollution in wastewater was significantly reduced with a decrease of about 98% in COD, 95% in TN, and 92% in TOC.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)