Article
Biochemistry & Molecular Biology
Natalie Gasterich, Sophie Wetz, Stefan Tillmann, Lena Fein, Anke Seifert, Alexander Slowik, Ralf Weiskirchen, Adib Zendedel, Andreas Ludwig, Steffen Koschmieder, Cordian Beyer, Tim Clarner
Summary: Astrocytes play a crucial role in responding to neurologic injuries and regulating immune responses, with LCN2 being involved in innate immune responses and iron regulation. However, studies suggest that LCN2 is not essential for basic astrocytic functions in the context of inflammation, but may have a regulatory effect on other cells like endothelial cells of the blood-brain barrier.
JOURNAL OF MOLECULAR NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Erica Staurenghi, Valentina Cerrato, Paola Gamba, Gabriella Testa, Serena Giannelli, Valerio Leoni, Claudio Caccia, Annalisa Buffo, Wendy Noble, Beatriz Gomez Perez-Nievas, Gabriella Leonarduzzi
Summary: Among the brain hallmarks of Alzheimer's disease, reactive astrocytes are correlated with neuronal loss and cognitive deficits, while alterations in brain cholesterol metabolism contribute to AD pathogenesis. Oxysterols in AD brains induce astrocyte reactivity and the release of mediators that impact neuronal health and synapses, with lipocalin-2 playing a key role in mediating the synaptotoxic effect of oxysterol-treated astrocytes.
Article
Biochemistry & Molecular Biology
Ye-Jin Cho, So-Hyun Park, Kwon-Yul Ryu
Summary: Astrocytes and microglia play crucial roles in maintaining brain homeostasis and the progression of neurological disorders. This study reveals that mild oxidative stress can downregulate the production of lipocalin-2 (LCN2) in glial cells, independently of the NF-kappa B signaling pathway. Additionally, Nrf2, another pathway involved in oxidative stress response, is not responsible for the downregulation of LCN2 in glial cells. These findings suggest that mild oxidative stress may be important in regulating LCN2 homeostasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Clinical Neurology
Jae-Hong Kim, Ri Jin Kang, Seung Jae Hyeon, Hoon Ryu, Hyejin Joo, Youngmin Bu, Jong-Heon Kim, Kyoungho Suk
Summary: Reactive glial cells play a role in brain injury, but their contribution to secondary inflammatory pathology and neurological deficits is poorly understood. This study investigates the role of Lipocalin-2 (LCN2) in neuroinflammation following brain injury. LCN2 expression is high in the mouse brain after controlled cortical impact (CCI) and photothrombotic stroke (PTS) injury. It is also significantly higher in patients with chronic traumatic encephalopathy (CTE). Astrocytes are the major source of LCN2 in the injured brain. Deficiency of LCN2 or injection of an LCN2 neutralizing antibody reduces brain lesions, behavioral deficits, and neuroinflammation. In cultured glial cells, LCN2 enhances proinflammatory cytokine gene expression and inhibits neurotropic factors expression. Overall, LCN2 mediates secondary damage response to traumatic and ischemic brain injury by promoting neuroinflammation and suppressing the expression of neurotropic factors.
Article
Biotechnology & Applied Microbiology
Dongming Wu, Xiaopeng Wang, Ye Han, Yayun Wang
Summary: The study demonstrated that LCN2 has an impact on apoptosis, which may contribute to the pathogenesis of metabolic diseases and cancer.
Review
Pharmacology & Pharmacy
Bi Deng, Zhirui Zhang, Huixuan Zhou, Xinran Zhang, Shuliang Niu, Xisheng Yan, Jie Yan
Summary: METH abuse is a significant global public health concern, and the mechanisms underlying its action are unclear. miRNAs play a role in the toxicity and addiction of METH after its use.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Neurosciences
Mandana Lotfi, Sohrab Kazemi, Anahita Ebrahimpour, Fereshteh Pourabdolhossein, Leila Satarian, Atiyeh Eghbali, Ali Akbar Moghadamnia
Summary: TQ from black seeds of Nigella sativa has a neuroprotective effect on NP-induced neurotoxicity by reducing oxidative damage and neuroinflammation.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Cell Biology
Byung-Kwon Jung, Yujin Park, Boran Yoon, Jin-Sil Bae, Seung-Woo Han, Ji-Eun Heo, Dong-Eun Kim, Kwon-Yul Ryu
Summary: LCN2 is a secretory protein produced by activated microglia and reactive astrocytes under neuroinflammatory stress conditions, leading to neuronal apoptosis. This study demonstrates that LCN2 secretion can be reduced by proteasome inhibition and autophagic flux activation in reactive astrocytes stimulated with lipopolysaccharide. The results suggest that modulating LCN2 production and secretion may offer a new therapeutic approach for neuroinflammatory diseases.
Article
Agriculture, Multidisciplinary
Ying-Xin Zhao, Yi-Xi Tang, Xiao-Han Sun, Shi-Yong Zhu, Xue-Yan Dai, Xue-Nan Li, Jin-Long Li
Summary: This study utilized an in vivo model of neurotoxicity in quail to investigate the effects of DEHP exposure on astrocytes. The results revealed that DEHP exposure resulted in phosphorylation and translocation of Cx43 in astrocytes. Additionally, the study showed that DEHP exposure induced pro-inflammatory status and autophagosome formation in astrocytes. These findings provide valuable insights into the mechanisms of neurodegeneration caused by DEHP exposure and suggest a potential therapeutic target for its treatment.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Engineering, Environmental
Mari Ochiai, Hoa Thanh Nguyen, Nozomi Kurihara, Masashi Hirano, Yuko Tajima, Tadasu K. Yamada, Hisato Iwata
Summary: Whales accumulate high levels of environmental pollutants, such as polychlorinated biphenyls (PCBs) and their metabolites, which may lead to abnormal behavior and neuronal dysfunction. This study reprogrammed neurons from fibroblasts of mass stranded melon-headed whales and found that exposure to PCB metabolite 4'OH-CB72 caused apoptosis and altered gene expressions associated with neurodegenerative diseases. The noninvasive approach using fibroblast-derived neurons proved useful for assessing neurotoxicity in whales.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hyun Joo Shin, Kyung Eun Kim, Eun Ae Jeong, Hyeong Seok An, So Jeong Lee, Jaewoong Lee, Gu Seob Roh
Summary: Type 2 diabetes is associated with an increased risk of Alzheimer's disease. Glial cell activation plays a crucial role in the development of neuroinflammation in both diabetes and AD. In a study with diabetic mice fed a high-fat diet, it was found that amyloid-beta oligomer treatment led to the retraction of microglial process and increased levels of galectin-3 and lipocalin-2 in the hippocampus, suggesting their involvement in amyloid toxicity mechanisms and glial activation under diabetic conditions.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Agriculture, Multidisciplinary
Ying-Xin Zhao, Xue-Nan Li, Yi-Xi Tang, Milton Talukder, Yi Zhao, Jin-Long Li
Summary: Cadmium is highly toxic and can be accumulated in the environment and throughout the food chain, posing risks to both humans and animals. This study aimed to uncover the neurological effects of cadmium and found that high doses of cadmium caused damage to the striatum in the brain, resulting in degeneration of neurons and dysfunction of astrocytes. Transcriptome analysis revealed that cadmium exposure transformed striatal astrocytes into the A1 state, which was found to be caused by the internalization of connexin 43. This study identifies connexin 43 internalization as the target of cadmium anchoring and suggests a new direction for studying the effects of cadmium on the nervous system and potential treatments for related diseases.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Neurosciences
Sergey Kalinin, Douglas L. Feinstein
Summary: This study found that conditional knockout of astrocyte-derived LCN2 in experimental autoimmune encephalomyelitis (EAE) delayed clinical scores and reduced astrocyte GFAP expression. Furthermore, decreased expression of oligodendrocyte proteolipid protein and transcription factor Olig2 was observed, suggesting that astrocyte LCN2 contributes to early events and reduces damage to mature oligodendrocytes in EAE.
NEUROSCIENCE LETTERS
(2023)
Article
Pharmacology & Pharmacy
Juliette Bedrossiantz, Marina Bellot, Pol Dominguez-Garcia, Melissa Faria, Eva Prats, Cristian Gomez-Canela, Raul Lopez-Arnau, Elena Escubedo, Demetrio Raldua
Summary: This study successfully established a zebrafish model of METH-induced neurotoxicity, demonstrating a concentration- and time-dependent decrease in dopamine, norepinephrine, and serotonin levels in METH-exposed fish. Behavioral changes included hyperactivity, positive geotaxis, and negative scototaxis, as well as social isolation, while transcriptional changes indicated alterations in gene expression related to neuronal injury.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Paola Lenzi, Francesca Biagioni, Carla L. Busceti, Gloria Lazzeri, Maico Polzella, Alessandro Frati, Michela Ferrucci, Francesco Fornai
Summary: Recent evidence suggests that methamphetamine (METH) causes mitochondrial alterations, leading to neurotoxicity. However, most studies have focused on mitochondrial activity rather than morphology. In this study, researchers used light and transmission electron microscopy to analyze dose-dependent mitochondrial structural changes during METH exposure. The findings indicate that METH-induced neurodegeneration is closely associated with specific mitochondrial damage, and a scoring system for mitochondrial integrity was established. Furthermore, the study revealed changes in mitochondrial proteins related to fission/mitophagy and mitochondrial dynamics. These results provide structural evidence that mitochondria are direct and indirect targets of METH-induced toxicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Cardiac & Cardiovascular Systems
Xuebing Chen, Qi Miao, Yangeng Yu, Fu Zhang, Leping Sun, Yunle Meng, Huijun Wang, Dongri Li
ANNALS OF THORACIC SURGERY
(2019)
Article
Nanoscience & Nanotechnology
Feng Qiu, Chunlei Yuan, Jianhua Xu, Junai Zhang, Fu Chen, Dongdong Liu, Biying Zheng, Ruixi Li, Xianzhang Huang, Junfa Xu
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2019)
Article
Neurosciences
Xuebing Chen, Jiancong Lu, Xu Zhao, Chuanxiang Chen, Dongfang Qiao, Huijun Wang, Xia Yue
FRONTIERS IN CELLULAR NEUROSCIENCE
(2019)
Article
Biochemistry & Molecular Biology
Xu Zhao, Jiancong Lu, Xuebing Chen, Zhengxiang Gao, Cui Zhang, Chuanxiang Chen, Dongfang Qiao, Huijun Wang
Summary: The study identified the molecular mechanisms of METH-induced neuronal programmed necrosis, showing that RIP3 plays a crucial role in the process and could be a potential therapeutic target for METH-induced neurotoxicity.
Article
Clinical Neurology
Xue-Bing Chen, Dong Qu, Qing Zhang, Xia Yue, Dong-Fang Qiao
Summary: This case study reports the death of a 30-year-old man due to acute pulmonary edema during rt-pa thrombolytic therapy, emphasizing the importance of closely monitoring vital sign changes and being aware of the risk of pulmonary edema during thrombolysis.
Review
Cell Biology
Yutong Wang, Feng Qiu, Yifan Xu, Xiaorui Hou, Zhili Zhang, Lei Huang, Huijun Wang, Hui Xing, Sha Wu
Summary: Stem cell-like memory T cells (Tscm) have characteristics that make them potential candidates for cancer immunotherapy and prevention, but limited understanding of their development process hinders their applications. Further research is needed to explore the molecular and cellular pathways involved in Tscm differentiation.
JOURNAL OF LEUKOCYTE BIOLOGY
(2021)
Article
Cell Biology
Jingshen Zhuang, Xuebing Chen, Guixing Cai, Dizheng Wu, Chen Tu, Siyuan Zhu, Yusheng Huang, Ping Xu, Zhaoming Zhong
Summary: Accumulation of AOPPs is associated with enhanced osteoclastogenesis and deterioration of bone microstructure in aged mice. In vitro experiments showed that AOPPs directly induce osteoclastogenesis by activating various signaling pathways, leading bone marrow monocytes to differentiate into mature osteoclasts.
CELL DEATH & DISEASE
(2021)
Article
Environmental Sciences
Xuebing Chen, Jingshen Zhuang, Qianling Chen, Luyao Xu, Xia Yue, Dongfang Qiao
Summary: This study investigated the toxic effects of PVC-MPs on mice, and found that PVC-MPs can cause intestinal injury and alter gut microbiome composition and metabolome profiles. The research is of great importance in assessing the health risks of PVC-MPs to humans.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Biochemical Research Methods
Xuebing Chen, Shengjie Nie, Liping Hu, Yating Fang, Wei Cui, Hui Xu, Congying Zhao, Bo-Feng Zhu
Summary: The aim of this study was to investigate the genetic characteristics and forensic application values of 57 autosomal InDel loci in the Chinese YM group. The results showed that these InDels were suitable for forensic individual identification and paternity testing. The genetic similarities between the YM group and East Asian populations were higher than that between the YM group and other geographical populations.
Review
Medicine, Legal
Qianling Chen, Xuebing Chen, Luyao Xu, Rui Zhang, Zhigang Li, Xia Yue, Dongfang Qiao
Summary: Traumatic brain injury (TBI) has high morbidity and poor prognosis, and traumatic axonal injury (TAI) is a common pathological change in the primary injury of TBI. However, the postmortem diagnosis of TAI-caused death is still challenging, with a lack of recognized diagnostic indicators. Therefore, studying the postmortem diagnosis of TAI is of great significance in forensic pathology.
FORENSIC SCIENCE MEDICINE AND PATHOLOGY
(2022)
Article
Environmental Sciences
Xuebing Chen, Jingshen Zhuang, Qianling Chen, Luyao Xu, Xia Yue, Dongfang Qiao
Summary: Exposure to polyvinyl chloride microplastics (PVC-MPs) causes liver injury and dysbiosis of gut microbiota in mice. Furthermore, PVC-MPs exposure leads to decreased levels of serum and hepatic triglyceride and total bile acid. RNA sequencing analysis of liver tissue identified differentially expressed genes associated with multiple pathways.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Review
Cell Biology
Luyao Xu, Lingyue Li, Qianling Chen, Yuebing Huang, Xuebing Chen, Dongfang Qiao
Summary: Methamphetamine (METH), a highly toxic amphetamine-type stimulant, has led to global addiction and various public health and safety issues. Recent studies have shown that non-coding RNA (ncRNA) plays a crucial role in METH addiction through different mechanisms. This article focuses on long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and circular RNAs (circRNAs), which are involved in neurotoxic effects of METH abuse such as cognitive impairment, behavioral abnormalities, and psychiatric disorders. Differential expression (DE) ncRNAs also suggest specific responses and sensitivity to METH neurotoxicity in different brain regions and cells. The findings provide new insights and strategies for the prevention and treatment of METH abuse.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hui Xu, Shengjie Nie, Liping Hu, Xuebing Chen, Ming Zhao, Xi Yuan, Yifeng Lin, Bofeng Zhu
Summary: The Chinese Yunnan Hani ethnic group was investigated using 57 autosomal InDel genetic markers and compared to 30 global reference populations. The results showed that this novel InDel multiplex amplification system could be used as a powerful tool for personal identification and paternity testing in the Chinese Yunnan Hani group.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2023)
