Article
Biochemistry & Molecular Biology
Kamila A. Kamarova, Natalia M. Ershova, Ekaterina V. Sheshukova, Eugene A. Arifulin, Natalia L. Ovsiannikova, Alexandra A. Antimonova, Andrei A. Kudriashov, Tatiana V. Komarova
Summary: In this study, four members of the RGP family in N. benthamiana were identified and characterized. The results demonstrate that NbRGP1-3 and 5 mRNA accumulation significantly increases upon tobacco mosaic virus (TMV) infection, and all class 1 NbRGPs inhibit TMV intercellular transport and replication. Additionally, elevated expression of NbRGP1-2 stimulates callose deposition at plasmodesmata and interacts with TMV movement protein (MP), resulting in reduced viral spread and replication.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Manikandan Veerabagu, Christiaan van der Schoot, Veronika Tureckova, Danuse Tarkowska, Miroslav Strnad, Paeivi L. H. Rinne
Summary: Perennial para- and endo-dormancy are seasonally separate phenomena. Para-dormancy is the suppression of axillary buds (AXBs) by a growing shoot, while endo-dormancy is the short-day elicited arrest of terminal and AXBs. ABA and GA play important roles in both phenomena. Blocking ABA biosynthesis reduced ABA levels, upregulated GA3ox-biosynthetic genes, and initiated branching, while blocking GA(4) biosynthesis promoted callose deposition and endo-dormancy. The results provide new insights into the roles of ABA and GA in dormancy cycling.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Agriculture, Multidisciplinary
Tiange Zhou, Ying He, Xiao Han, Qian Sun, Yuan Hu Xuan
Summary: Rice sheath blight is a serious disease that affects rice production, but the mechanisms of defense are poorly understood. This study found that the expression levels of beta-glucanase family genes are responsive to infection and positively regulate rice resistance to sheath blight. OsBGL2 was also found to co-localize with AtPDCB1 and limit the permeability of plasmodesmata. The identification and functional elucidation of these genes help fill the gap in understanding the mechanism of plasmodesmata permeability in rice sheath blight resistance.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Plant Sciences
Shuai Fang, Xiaoguang Shang, Qingfei He, Weixi Li, Xiaohui Song, Baohong Zhang, Wangzhen Guo
Summary: A cell wall-localized β-1,3-glucanase, GhGLU18, enhances polysaccharide metabolism and cell wall synthesis to promote fiber cell elongation and thickening in cotton. GhGLU18 specifically expressed in fibers and plays a role in fiber cell wall development.
Review
Plant Sciences
Liam German, Richa Yeshvekar, Yoselin Benitez-Alfonso
Summary: Cell walls are critical for plant growth and development, providing support and protection. Callose, a type of glucan, accumulates in specialized cell wall microdomains, including plasmodesmata. It regulates important biological processes and affects the transport of signaling proteins and RNA molecules. The mechanisms controlling callose synthesis and degradation are still unresolved. This review explores recent literature on callose metabolism and its response to mutualistic symbionts and pathogenic elicitors.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Plant Sciences
Chengliang Wang, Yuanyuan Lyu, Qi Zhang, Hongye Guo, Defu Chen, Xiwen Chen
Summary: This study reports a glycosylphosphatidylinositol-anchored beta-1,3-glucanase, BG14, which degrades callose in seed embryos and plays important roles in seed longevity and dormancy in Arabidopsis.
Article
Biology
Philip Kirk, Sam Amsbury, Liam German, Rocio Gaudioso-Pedraza, Yoselin Benitez-Alfonso
Summary: Our study identified new proteins and regulatory mechanisms affecting plasmodesmata function using proteomic meta-analysis and transcriptomic data. We also developed a freely accessible pipeline for screening experimental proteomes and predicting plasmodesmata proteins in different plant species.
Article
Plant Sciences
Ekaterina V. Sheshukova, Natalia M. Ershova, Kamila A. Kamarova, Yuri L. Dorokhov, Tatiana V. Komarova
FRONTIERS IN PLANT SCIENCE
(2020)
Review
Engineering, Chemical
Yuri L. Dorokhov, Ekaterina V. Sheshukova, Tatiana E. Byalik, Tatiana V. Komarova
Review
Biochemistry & Molecular Biology
Fedor A. Lipskerov, Ekaterina V. Sheshukova, Tatiana V. Komarova
Summary: Formaldehyde (FA) is a simple and highly reactive compound that exists in the environment and living organisms, capable of causing protein crosslinking and DNA damage. Novel instruments and approaches are needed to monitor and measure FA content in various environmental and biological samples. Genetically encoded FA-sensors and chemically-synthesized probes have been developed for in vivo and in cellulo FA monitoring.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Ekaterina Sheshukova, Natalia M. Ershova, Fedor A. Lipskerov, Tatiana Komarova
Summary: Plants can be used as a promising platform for producing recombinant proteins. This study proposes a novel method to enhance the synthesis of recombinant proteins using viral vectors. The approach suggests that introducing foreign nuclear proteins, including bacterial effectors, can interfere with the import of cellular nuclear proteins and trigger antibacterial defense reactions, thereby creating favorable conditions for cytoplasmic virus reproduction. In this study, an artificial nuclear protein mRFP:NLS was synthesized in plant cells, mimicking a bacterial effector. The synthesis of mRFP:NLS resulted in the accumulation of Nb gamma Thio mRNA and stimulated the reproduction of a viral vector based on the genome of crucifer-infecting tobacco mosaic virus (crTMV) in plant leaves. The study also identified a gene promoter, Pr-gamma Thio, which showed increased activity in response to the increased synthesis of GFP fused with NLS.
Article
Plant Sciences
Natalia Ershova, Ekaterina Sheshukova, Kamila Kamarova, Evgenii Arifulin, Vadim Tashlitsky, Marina Serebryakova, Tatiana Komarova
Summary: Plant viruses infect their hosts by inducing KPILP gene expression to regulate the chloroplast retrograde signaling system and affect carbon partitioning and plasmodesmata permeability.
FRONTIERS IN PLANT SCIENCE
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
E. Sheshukova, T. Komarova, N. Ershova, K. Kamarova, Y. Dorokhov
Meeting Abstract
Biochemistry & Molecular Biology
N. Ershova, E. Sheshukova, T. Komarova, K. Kamarova, Y. Dorokhov
Meeting Abstract
Biochemistry & Molecular Biology
K. Kamarova, E. Sheshukova, T. Komarova, N. Ershova, Y. Dorokhov
Article
Chemistry, Multidisciplinary
K. M. Konoplina, E. N. Kosobokova, E. V. Sheshukova, M. V. Pinyugina, A. A. Malchenkova, V. S. Kosorukov
MOSCOW UNIVERSITY CHEMISTRY BULLETIN
(2020)
