CRISPR interference (CRISPRi) as transcriptional repression tool for Hungateiclostridium thermocellum DSM 1313

Hijacking CRISPR-Cas for high-throughput bacterial metabolic engineering: advances and prospects

(2018) Ioannis Mougiakos et al.   CURRENT OPINION IN BIOTECHNOLOGY

Deletion of the Clostridium thermocellum recA gene reveals that it is required for thermophilic plasmid replication but not plasmid integration at homologous DNA sequences

(2018) Joseph Groom et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Recent Developments of the Synthetic Biology Toolkit for Clostridium

(2018) Rochelle C. Joseph et al.   Frontiers in Microbiology

Enhanced solvent production by metabolic engineering of a twin-clostridial consortium

(2017) Zhiqiang Wen et al.   METABOLIC ENGINEERING

Engineering electron metabolism to increase ethanol production in Clostridium thermocellum

(2017) Jonathan Lo et al.   METABOLIC ENGINEERING

Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase

(2017) Liang Tian et al.   Microbial Cell Factories

High‐throughput CRISPRi phenotyping identifies new essential genes in Streptococcus pneumoniae

(2017) Xue Liu et al.   Molecular Systems Biology

Characterizing a thermostable Cas9 for bacterial genome editing and silencing

(2017) Ioannis Mougiakos et al.   Nature Communications

Extending CRISPR-Cas9 Technology from Genome Editing to Transcriptional Engineering in the Genus Clostridium

(2016) Mark R. Bruder et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Genetic engineering of Clostridium thermocellum DSM1313 for enhanced ethanol production

(2016) Saranyah Kannuchamy et al.   BMC BIOTECHNOLOGY

A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria

(2016) Jason M. Peters et al.   CELL

Depletion of Undecaprenyl Pyrophosphate Phosphatases Disrupts Cell Envelope Biogenesis in Bacillus subtilis

(2016) Heng Zhao et al.   JOURNAL OF BACTERIOLOGY

Enhanced protein and biochemical production using CRISPRi-based growth switches

(2016) Songyuan Li et al.   METABOLIC ENGINEERING

Bacterial Genome Editing with CRISPR-Cas9: Deletion, Integration, Single Nucleotide Modification, and Desirable “Clean” Mutant Selection in Clostridium beijerinckii as an Example

(2016) Yi Wang et al.   ACS Synthetic Biology

Elimination of hydrogenase active site assembly blocks H2 production and increases ethanol yield in Clostridium thermocellum

(2015) Ranjita Biswas et al.   Biotechnology for Biofuels

Elimination of formate production in Clostridium thermocellum

(2015) Thomas Rydzak et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Elimination of metabolic pathways to all traditional fermentation products increases ethanol yields in Clostridium thermocellum

(2015) Beth Papanek et al.   METABOLIC ENGINEERING

Updates on the sporulation process in Clostridium species

(2015) Prabhat K. Talukdar et al.   RESEARCH IN MICROBIOLOGY

Multiple Gene Repression in Cyanobacteria Using CRISPRi

(2015) Lun Yao et al.   ACS Synthetic Biology

Cas9-Based Tools for Targeted Genome Editing and Transcriptional Control

(2014) Tao Xu et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Expansion of the genetic toolkit for metabolic engineering of Clostridium pasteurianum: chromosomal gene disruption of the endogenous CpaAI restriction enzyme

(2014) Michael E Pyne et al.   Biotechnology for Biofuels

Genome engineering and gene expression control for bacterial strain development

(2014) Chan Woo Song et al.   Biotechnology Journal

Increase in Ethanol Yield via Elimination of Lactate Production in an Ethanol-Tolerant Mutant of Clostridium thermocellum

(2014) Ranjita Biswas et al.   PLoS One

The emergence of Clostridium thermocellum as a high utility candidate for consolidated bioprocessing applications

(2014) Hannah Akinosho et al.   Frontiers in Chemistry

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Characterization of Clostridium thermocellum strains with disrupted fermentation end-product pathways

(2013) Douwe van der Veen et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9

(2013) Jian-Feng Li et al.   NATURE BIOTECHNOLOGY

CRISPR interference (CRISPRi) for sequence-specific control of gene expression

(2013) Matthew H Larson et al.   Nature Protocols

A Targetron System for Gene Targeting in Thermophiles and Its Application in Clostridium thermocellum

(2013) Georg Mohr et al.   PLoS One

Improved lignocellulose conversion to biofuels with thermophilic bacteria and thermostable enzymes

(2012) Aditya Bhalla et al.   BIORESOURCE TECHNOLOGY

Role of the CipA Scaffoldin Protein in Cellulose Solubilization, as Determined by Targeted Gene Deletion and Complementation in Clostridium thermocellum

(2012) D. G. Olson et al.   JOURNAL OF BACTERIOLOGY

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

High Ethanol Titers from Cellulose by Using Metabolically Engineered Thermophilic, Anaerobic Microbes

(2011) D. Aaron Argyros et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum

(2011) S. D. Brown et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Development of pyrF-Based Genetic System for Targeted Gene Deletion in Clostridium thermocellum and Creation of a pta Mutant

(2010) S. A. Tripathi et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Deletion of the Cel48S cellulase fromClostridium thermocellum

(2010) Daniel G. Olson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA