Article
Neurosciences
Mohammad A. Ghane, Wei Wei, Dina W. Yakout, Zachary D. Allen, Cassandra L. Miller, Bin Dong, Jenny J. Yang, Ning Fang, Angela M. Mabb
Summary: The study found that disrupting Arc protein ubiquitination enhances DHPG-induced ER-mediated Ca2+ release. This alteration is observed in all neuronal subregions except secondary branchpoints. Disruptions in Arc ubiquitination increase Arc interaction with the integral ER protein Calnexin.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Neurosciences
Alexandre Bouron
Summary: The endoplasmic reticulum (ER) is the primary storage compartment for calcium (Ca2+) in eukaryotic cells. Ca2+ is mobilized from the ER through store-operated Ca2+ channels (SOCCs), leading to a store-operated Ca2+ entry (SOCE) that has been extensively studied in non-excitable cells. The presence of this Ca2+ entry route in neurons has been a subject of debate, but experimental evidence suggests that SOCE can be generated by the recruitment of Ca2+ from neuronal ER Ca2+ stores. This review summarizes the main supporting studies and explores the molecular composition, expression, pharmacological properties, and physiological relevance of neuronal SOCCs.
MOLECULAR NEUROBIOLOGY
(2023)
Review
Cell Biology
Dong-Xu Huang, Xin Yu, Wen-Jun Yu, Xin-Min Zhang, Chang Liu, Hong-Ping Liu, Yue Sun, Zi-Ping Jiang
Summary: The precise regulation of calcium ion by neurons is essential for cellular signaling and survival, with calcium homeostasis maintained by both endoplasmic reticulum and mitochondria. Dysregulation of calcium signaling is associated with Alzheimer's disease, highlighting the importance of understanding the cellular and molecular mechanisms underlying calcium regulation in neurodegenerative diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Yupeng Xiao, Zhao Li, Alberto Bianco, Baojin Ma
Summary: This review focuses on the application of calcium ion overload in cancer therapy. Calcium ion overload can induce apoptosis and can be enhanced through different strategies, including surface modification and optimization of nanomaterials, increasing intracellular H+ levels and photothermal effects, and regulating the influx and efflux of calcium ions.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Multidisciplinary Sciences
Mate Katona, Adam Bartok, Zuzana Nichtova, Gyorgy Csordas, Elena Berezhnaya, David Weaver, Arijita Ghosh, Peter Varnai, David Yule, Gyorgy Hajnoczky
Summary: The study reveals that the IP3 receptor traffics in and out of ER-Mitochondria contacts, and when trapped, enhances calcium signaling to stimulate energy metabolism.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Shanliang Zheng, Dong Zhao, Guixue Hou, Song Zhao, Wenxin Zhang, Xingwen Wang, Li Li, Liang Lin, Tie-Shan Tang, Ying Hu
Summary: The study demonstrates the interaction between TMCO1 and iASPP in colon cancer, affecting tumor growth and cell apoptosis by modulating Ca2+ homeostasis. Intervention in the iASPP-TMCO1 axis can effectively inhibit tumor progression.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Pharmacology & Pharmacy
Fenglan Chu, Hanxing Wan, Weidong Xiao, Hui Dong, Muhan Lue
Summary: Calcium signaling is crucial for intestinal glutamine (Gln) transport, and multiple plasma membrane Ca2+-permeable channels and transporters are involved in this process.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Adalberto Merighi, Laura Lossi
Summary: In addition to protein processing, the endoplasmic reticulum (ER) also plays roles in lipid synthesis, molecular transfers, and Ca2+ homeostasis. Proper protein folding and modification in the ER require molecular chaperones and a favorable environment. Disruption in ER conditions or chaperone capacity can lead to the unfolded protein response (UPR), triggering autophagy and potentially causing cell death.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Zi-He Wang, Zi-Fang Shen, Jing-Yi Wang, Ying-Ying Cai, Lin Li, Jian Liao, Jian-Ping Lu, Xue-Ming Zhu, Fu-Cheng Lin, Xiao-Hong Liu
Summary: This study identified a novel calcineurin regulatory-subunit-binding protein, MoCbp7, which is involved in calcium signal transduction and is important for the growth, conidiation, appressorium formation, invasive growth, and virulence of Magnaporthe oryzae. MoCbp7 also interacts with calcineurin to regulate endoplasmic reticulum homeostasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Ruiyuan Xu, Jianlu Song, Rexiati Ruze, Yuan Chen, Xinpeng Yin, Chengcheng Wang, Yupei Zhao
Summary: This study reveals squalene epoxidase (SQLE) as a novel oncogene that promotes pancreatic cancer (PC) growth by mitigating endoplasmic reticulum stress and activating lipid raft-regulated Src/PI3K/Akt signaling pathway. SQLE facilitates cell proliferation, induces cell cycle progression, and inhibits apoptosis in vitro, while promoting tumor growth in vivo. SQLE inhibitors effectively suppress PC cell proliferation and xenograft tumor growth, highlighting the potential of SQLE as a therapeutic target for PC.
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Benjamin Cartes-Saavedra, Josefa Macuada, Daniel Lagos, Duxan Arancibia, Maria E. Andres, Patrick Yu-Wai-Man, Gyoergy Hajnoczky, Veronica Eisner
Summary: Autosomal Dominant Optic Atrophy (ADOA), caused by OPA1 mutations, affects Ca2+ homeostasis by modulating ER-mitochondria coupling, potentially contributing to the progression of ADOA.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Lauren C. Panzera, Ben Johnson, Josiah A. Quinn, In Ha Cho, Michael M. Tamkun, Michael B. Hoppa
Summary: Research indicates that Kv2.1 channels play a crucial role in facilitating ER calcium uptake during electrical activity in neurons, which is related to synaptic transmission. Loss of the Kv2.1 channel can disrupt synaptic vesicle fusion during stimulation, while its non-conducting role can link ER calcium uptake with electrical activity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Anatoliy Shmygol, Jan J. Brosens
Summary: Embryo implantation is a complex and tightly regulated process in humans, where uterine luminal epithelium functions as a biosensor to gauge embryo quality and transmit information to the endometrial stromal cells, ensuring only high quality embryos are implanted. Trypsin, secreted by the blastocyst, has been implicated in this crosssignaling, triggering intracellular calcium signaling in uterine epithelium through protease-activated G-protein coupled receptors and activation of epithelial sodium channels.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Stephanie M. Cicalese, Josiane Fernandes da Silva, Fernanda Priviero, R. Clinton Webb, Satoru Eguchi, Rita C. Tostes
Summary: Cells respond to stress by activating defense signaling pathways for survival or cell death. Arterial hypertension and aging share mechanisms that mediate chronic cell stress, potentially leading to unresolved pathologies.
CIRCULATION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
David M. Booth, Peter Varnai, Suresh K. Joseph, Gyorgy Hajnoczky
Summary: The study shows that individual mitochondria generate oxidative bursts at interorganellar contact sites, with Ca2+ channels directly sensing these bursts and responding with transient Ca2+ transients. Under mitochondrial stress or apoptotic stimuli, the frequency and amplitude of the oxidative bursts can be increased.
Article
Biophysics
Oksana Iamshanova, Pascal Mariot, V'yacheslav Lehen'kyi, Natalia Prevarskaya
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2016)
Article
Oncology
Charlotte Dubois, Fabien Vanden Abeele, V'yacheslav Lehen'kyi, Dimitra Gkika, Basma Guarmit, Gilbert Lepage, Christian Slomianny, Anne Sophie Borowiec, Gabriel Bidaux, Mohamed Benahmed, Yaroslav Shuba, Natalia Prevarskaya
Article
Cardiac & Cardiovascular Systems
Dmitri Gordienko, Oleksandr Povstyan, Khrystyna Sukhanova, Maylis Raphael, Maksym Harhun, Yulia Dyskina, V'yacheslav Lehen'kyi, Abdirahman Jama, Zhi-Liang Lu, Roman Skryma, Natalia Prevarskaya
CARDIOVASCULAR RESEARCH
(2015)
Article
Multidisciplinary Sciences
Maylis Raphael, V'yacheslav Lehen'kyi, Matthieu Vandenberghe, Benjamin Beck, Sergiy Khalimonchyk, Fabien Vanden Abeele, Leonardo Farsetti, Emmanuelle Germain, Alexandre Bokhobza, Adriana Mihalache, Pierre Gosset, Christoph Romanin, Philippe Clezardin, Roman Skryma, Natalia Prevarskaya
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2014)
Article
Materials Science, Biomaterials
Alexandre Barras, Nadia Skandrani, Mariano Gonzalez Pisfil, Solomiya Paryzhak, Tetiana Dumych, Aurelien Haustrate, Laurent Heliot, Tijani Gharbi, Hatem Boulahdour, V'yacheslav Lehen'kyi, Rostyslav Bilyy, Sabine Szunerits, Gabriel Bidaux, Rabah Boukherroub
JOURNAL OF MATERIALS CHEMISTRY B
(2018)
Article
Chemistry, Physical
Fakhrossadat Mohammadi, Amin Sahraei, Chengnan Li, Aurelien Haustrate, V'yacheslav Lehen'kyi, Alexandre Barras, Rabah Boukherroub, Sabine Szunerits
JOURNAL OF PHYSICAL CHEMISTRY C
(2019)
Review
Cell Biology
Aurelien Haustrate, Aline Hantute-Ghesquier, Natalia Prevarskaya, Vyacheslav Lehen'kyi
Review
Pharmacology & Pharmacy
Aurelien Haustrate, Aline Hantute-Ghesquier, Natalia Prevarskaya, V'yacheslav Lehen'kyi
FRONTIERS IN PHARMACOLOGY
(2019)
Review
Cell Biology
Goncalo Mesquita, Natalia Prevarskaya, Albrecht Schwab, V'yacheslav Lehen'kyi
Summary: TRP channels, a diverse superfamily of ion channels, have been implicated in various physiological processes and their potential role in cancer progression, particularly in pancreatic cancer, is being explored. This review analyzes existing knowledge on TRP channels in cancer, with a focus on potential therapeutic interventions for pancreatic ductal adenocarcinoma.
Review
Oncology
Clement Cordier, Natalia Prevarskaya, V'yacheslav Lehen'kyi
Summary: Breast cancer is a major health concern for women worldwide. The TRPM7 ion channel has been identified as a potential target for diagnosis and treatment of breast cancer, with its overexpression potentially indicating a poor prognosis. Researchers are exploring the modulation of TRPM7 as a therapeutic target to inhibit proliferation, migration, and invasion in breast cancer.
Article
Biochemistry & Molecular Biology
Aurelien Haustrate, Adriana Mihalache, Clement Cordier, Pierre Gosset, Natalia Prevarskaya, V'yacheslav Lehen'kyi
Summary: Despite the early discovery of TRPV6 channel expression in various tissues in the early 2000s, there is still a lack of reliable tools for detecting its protein in different cells and tissues. We have developed and validated rabbit polyclonal anti-TRPV6 channel antibodies (rb79-82) against four epitopes of 15 amino acids. Among them, rb79 was capable of detecting the full-length glycosylated form of TRPV6 channel. This antibody is suitable for various in vitro applications and particularly useful for immunohistochemistry in cancer resection specimens.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Antoine Folcher, Dmitri Gordienko, Oksana Iamshanova, Alexandre Bokhobza, George Shapovalov, Dheeraj Kannancheri-Puthooru, Pascal Mariot, Laurent Allart, Emilie Desruelles, Corentin Spriet, Raquel Diez, Thibauld Oullier, Severine Marionneau-Lambot, Lucie Brisson, Sandra Geraci, Hathaichanok Impheng, V'yacheslav Lehen'kyi, Aurelien Haustrate, Adriana Mihalache, Pierre Gosset, Stephanie Chadet, Stephanie Retif, Maryline Laube, Julien Sobilo, Stephanie Lerondel, Giulia Villari, Guido Serini, Alessandra Fiorio Pla, Sebastien Roger, Gaelle Fromont-Hankard, Mustafa Djamgoz, Philippe Clezardin, Arnaud Monteil, Natalia Prevarskaya
Summary: There is increasing evidence that ion channels play a crucial role in the invasiveness and metastasis of cancer cells. However, the molecular mechanisms of ion signaling in promoting cancer behavior are not well understood, and the complex remodeling during metastasis remains to be explored. This study demonstrates that metastatic prostate cancer cells develop a specific Na+/Ca2+ signaling, which is essential for persistent invasion. The Na+ leak channel, NALCN, is identified as a major initiator and regulator of Ca2+ oscillations required for the formation of invadopodia. The signaling cascade involving NALCN, ion transport proteins, and proto-oncogene Src kinase promotes actin remodeling, secretion of proteolytic enzymes, and enhances cancer cell invasive potential and metastatic lesions in vivo.
Article
Oncology
Aurelien Haustrate, George Shapovalov, Corentin Spriet, Clement Cordier, Artem Kondratskyi, Lucile Noyer, Francois Foulquier, Natalia Prevarskaya, V'yacheslav Lehen'kyi
Summary: The upregulation of TRPV6 channel in cancer is associated with poor prognosis, but there is no reliable tool to target it. Two antibodies have been generated that can bind to TRPV6 and transiently activate calcium currents. This activation leads to apoptosis induction in prostate cancer cells, with only cells lacking TRPV6 expression being able to avoid death. Therefore, activating the TRPV6 channel may be more prospective than inhibiting it.
Review
Chemistry, Medicinal
Aline Hantute-Ghesquier, Aurelien Haustrate, Natalia Prevarskaya, V'yacheslav Lehen'kyi
