Article
Medicine, Research & Experimental
Xiao-Jing Song, Lei Zhang, Qiang Li, Ying Li, Fang-Hui Ding, Xun Li
Summary: The study showed that exosomal miR-124 derived from MICR-MSC could promote rat liver regeneration after PH by downregulating Foxg1 and inhibiting liver injury.
Article
Endocrinology & Metabolism
Lantian Wang, Xiawei Li, Jian Wu, Qiang Tang
Summary: Exosomal miRNA-let-7b-5p derived from pancreatic cancer cells promotes insulin resistance in C2C12 myotube cells by targeting RNF20 and activating the STAT3/FOXO1 axis.
DIABETES METABOLIC SYNDROME AND OBESITY
(2023)
Article
Biotechnology & Applied Microbiology
Fang Cheng, Fuji Yang, Yanjin Wang, Jing Zhou, Hui Qian, Yongmin Yan
Summary: This study investigates the efficacy and underlying mechanisms of human umbilical cord-derived exosomes (MSC-ex) in LOXL2 inhibition of liver fibrosis. It was found that MSC-ex could suppress LOXL2 expression through exosomal miR-27b-3p-mediated YAP down-regulation, thus delaying the progression of CCl4-induced liver fibrosis. These findings improve our understanding of the role of MSC-ex in liver fibrosis alleviation and provide new opportunities for clinical treatment.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Gastroenterology & Hepatology
Yihan Xu, Wenwei Qian, Liangyu Huang, Weiwei Wen, Yi Li, Feilong Guo, Zhenxing Zhu, Zhun Li, Jianfeng Gong, Zeqian Yu, Yan Zhou, Nan Lu, Weiming Zhu, Zhen Guo
Summary: Adherent-invasive Escherichia coli (AIEC) and intestinal macrophages play a role in the development of Crohn's disease (CD), particularly in the pathogenesis of intestinal fibrosis. This study found that AIEC colonization in the ileum is associated with increased intestinal fibrosis and reduced let-7b expression in CD patients. AIEC-infected mice also showed more severe intestinal fibrosis, which was improved by exogenous let-7b. Mechanistically, let-7b targets intestinal macrophages and inhibits TGF beta R1 to prevent fibrotic phenotype. AIEC suppresses exosomal let-7b from intestinal epithelial cells, promoting fibrotic macrophage polarization. In summary, AIEC contributes to intestinal fibrosis in CD, highlighting the potential of anti-AIEC or let-7b therapy for ameliorating fibrosis.
Article
Multidisciplinary Sciences
Katja Koeppen, Amanda Nymon, Roxanna Barnaby, Laura Bashor, Zhongyou Li, Thomas H. Hampton, Amanda E. Liefeld, Fred W. Kolling, Ian S. LaCroix, Scott A. Gerber, Deborah A. Hogan, Swetha Kasetty, Carey D. Nadell, Bruce A. Stanton
Summary: The study demonstrates that microRNAs in extracellular vesicles secreted by human airway epithelial cells can regulate protein expression, antibiotic sensitivity, and biofilm formation by Pseudomonas aeruginosa. This bidirectional interdomain communication provides direct evidence for transfer of miRNAs in EVs from eukaryotic cells to prokaryotes, resulting in phenotypic alterations in the prokaryote. The findings suggest potential clinical applications for using let-7b-5p and a beta-lactam antibiotic in nanoparticles or EVs to benefit patients with antibiotic-resistant P. aeruginosa infections.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Delphine Garnier, Edward Ratcliffe, Josephine Briand, Pierre-Francois Cartron, Lisa Oliver, Francois M. Vallette
Summary: Glioblastoma stem cells (GSCs) can activate mesenchymal stem cells (MSCs) into cancer-associated fibroblasts (CAFs) via extracellular vesicles (EVs) and promote tumor progression through miRNA-mediated mechanisms.
Article
Cell Biology
Virginia Egea, Kai Kessenbrock, Devon Lawson, Alexander Bartelt, Christian Weber, Christian Ries
Summary: Bone marrow-derived human mesenchymal stem cells contribute to tissue repair by recruiting to damaged or inflamed tissues and releasing factors that influence target cells. The microRNA let-7f plays a crucial role in regulating hMSC invasion in response to inflammation and hypoxia, and its exosomal form exhibits paracrine anti-tumor effects.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Yin Zhuang, Sheng Song, Dan Xiao, Xueguang Liu, Xiaofei Han, Shihao Du, Yuan Li, Yanming He, Shujun Zhang
Summary: The pathogenesis of intervertebral disc degeneration is still unclear. In this study, it was found that exosomes secreted by degenerative intervertebral disc-derived nucleus pulposus stem cells could exacerbate annulus fibrosus cell degeneration. This effect was mediated by the let-7b-5p miRNA, which inhibited cell proliferation, migration, and matrix synthesis, and promoted apoptosis. The activation of the IGF1R expression and PI3K-Akt pathway were also inhibited by let-7b-5p.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Caiyu Qiu, Chuanxiang Li, Quan Zheng, Si Fang, Jianqun Xu, Hongjuan Wang, Hongrong Guo
Summary: AFAP1-AS1 plays a pro-tumor role in lung cancer and is involved in the anticancer activity of Met in the treatment of lung adenocarcinoma. Met inhibits the malignant characteristics of lung cancer cells by regulating the AFAP1-AS1/miR-3163/SPP1/PI3K/Akt/mTOR axis.
Article
Oncology
Lu Zong, Pu Huang, Qing Song, Yan Kang
Summary: The study showed that BMSCs-exos can reduce inflammation by promoting M2 polarization, inhibiting M1 polarization, and resisting LPS-induced cytotoxicity and apoptosis. The long noncoding RNA H19 in BMSCs-exos modulates inflammation and neuroprotective effects by sponging miR-29b-3p.
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Yanyan Gao, Xuefeng Zhang, Tao Meng
Summary: The study investigated the molecular mechanisms by which let-7b regulates placental trophoblast functions and examined its expression in pre-eclampsia. Overexpression of let-7b in trophoblasts promoted cell proliferation and invasion, suppressed apoptosis and autophagy, and modulated inflammatory cytokine expression. The beneficial effects of let-7b were mediated through the ERK1/2 signaling pathway, suggesting let-7b as a potential biomarker for the diagnosis and treatment of pre-eclampsia.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2022)
Article
Oncology
Guang Shan, Xike Zhou, Juan Gu, Daoping Zhou, Wei Cheng, Huaiguo Wu, Yueping Wang, Tian Tang, Xuedong Wang
Summary: Exosomes derived from cancer-associated fibroblasts (CAFs) play a crucial role in promoting the metastasis, EMT, and drug resistance of bladder cancer cells through delivering miR-148b-3p. PTEN has been identified as a direct target of miR-148b-3p, and its overexpression can inhibit EMT, metastasis, and chemoresistance in bladder cancer cells. Downregulation of miR-148b-3p in CAF-derived exosomes may offer potential therapeutic opportunities for bladder cancer treatment by inhibiting the Wnt/beta-catenin pathway and promoting PTEN expression.
Article
Oncology
Shufang Han, Jie Fang, Lili Yu, Bin Li, Yuhong Hu, Ruimin Chen, Changyong Li, Chuanxu Zhao, Jiaying Li, Yinan Wang, Yuqi Gao, Hong Tan, Qun Jin
Summary: The study identified 10 differentially expressed miRNAs in patients with coronary heart disease and hyperglycemia, with functional analysis showing enrichment in cellular processes, single-organism processes, and biological regulation. Pathway analysis revealed enrichment in the mTOR signaling pathway, FoxO signaling pathway, and neurotrophin signaling pathway.
MOLECULAR MEDICINE REPORTS
(2023)
Article
Cell & Tissue Engineering
Ying Xie, Shan Tian
Summary: The study reveals that miRNA-325 derived from MSC-exos promotes trophoblast cell invasion and migration in PE by inhibiting let-7b expression and upregulating FOXO1.
JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING
(2022)