Article
Biochemistry & Molecular Biology
Prapansak Srisapoome, Kubpaphas Thummabancha, Ratree Wongpanya
Summary: This study successfully cloned and characterized two cDNAs encoding Nile tilapia DnaJ proteins, revealing their gene structures and tissue expression patterns under normal and pathogenic infection conditions. Silencing of these genes confirmed their crucial roles in response to heat and bacterial stress, suggesting their involvement in regulating intracellular proteins during infection.
Article
Biochemistry & Molecular Biology
Tanya Ju-Ngam, Nichanun McMillan, Mamoru Yoshimizu, Hisae Kasai, Ratree Wongpanya, Prapansak Srisapoome
Summary: This research conducted molecular characterization and biofunctional analyses of Hsp40 and Hsp90 genes in giant river prawns under various stress conditions. The highest similarity scores of these genes were found with crustaceans. Expression patterns of Mr-hsp40 and Mr-hsp90 were different under Aeromonas hydrophila challenge and heat-cold shock conditions, highlighting their significant roles in stress responses.
Article
Environmental Sciences
Weijie Yan, Yingjie Qiao, Jiangbo Qu, Xiumei Liu, Quanqi Zhang, Xubo Wang
Summary: Heat shock proteins, particularly the hsp40 subfamily, play important roles in developmental stages and stress responses in cells. A comprehensive analysis of hsp40 in Japanese flounder revealed that they are conservative during evolution, express in different developmental stages, and may act as sentinels in healthy organisms. Additionally, hsp40 genes in Japanese flounder showed differential expression patterns after a bacterial challenge, indicating their potential role in immune responses.
FRONTIERS IN MARINE SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Bianca Nitzsche, Michael Hoepfner, Bernhard Biersack
Summary: Heat shock protein 70 (Hsp70) and its interaction with Hsp90 play a significant role in cancer diseases. The Hsp70-Hsp40 axis, formed by the connection between Hsp70 and a smaller heat shock protein Hsp40, is a suitable target for designing anticancer drugs. This review discusses the current state and recent developments in the field of (semi-)synthetic small molecule inhibitors targeting Hsp70 and Hsp40, as well as their potential in medicinal chemistry and anticancer therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Zhi Huang, Dandan Wang, Jinrui Zhou, Hong He, Cong Wei
Summary: Modified FISH technique can improve the weak or absent fluorescence signals of microbes, enabling a more precise identification and understanding of microbes in complex samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Julia Ring, Jelena Tadic, Selena Ristic, Michael Poglitsch, Martina Bergmann, Nemanja Radic, Dirk Mossmann, Yongtian Liang, Marta Maglione, Andrea Jerkovic, Roozbeh Hajiraissi, Marcel Hanke, Victoria Kuettner, Heimo Wolinski, Andreas Zimmermann, Lana Domuz Trifunovic, Leonie Mikolasch, Daiana N. Moretti, Filomena Broeskamp, Julia Westermayer, Claudia Abraham, Simon Schauer, Christopher Dammbrueck, Sebastian J. Hofer, Mahmoud Abdellatif, Guido Grundmeier, Guido Kroemer, Ralf J. Braun, Niklas Hansen, Cornelia Sommer, Mirjana Ninkovic, Sandra Seba, Patrick Rockenfeller, Friederike-Nora Voegtle, Joern Dengjel, Chris Meisinger, Adrian Keller, Stephan J. Sigrist, Tobias Eisenberg, Frank Madeo
Summary: In this study, the researchers used yeast and fruit fly models to investigate the role of HSP40 family member Ydj1 in Alzheimer's disease. They found that Ydj1 physically interacts with Abeta42, stabilizes Abeta42 oligomers, and facilitates their translocation to mitochondria, resulting in cell death and memory impairment.
EMBO MOLECULAR MEDICINE
(2022)
Review
Microbiology
Sue Wickner, Thu-Lan Lily Nguyen, Olivier Genest
Summary: Heat shock protein 90 (Hsp90) is a molecular chaperone that folds and remodels proteins, regulating the activity of substrate proteins. Bacterial Hsp90 collaborates with Hsp70 and its cochaperones for protein remodeling, while eukaryotic Hsp90 involves over 20 cochaperones in addition to Hsp70. Further exploration of the bacterial chaperone system may provide insights into the complex eukaryotic Hsp90 system.
ANNUAL REVIEW OF MICROBIOLOGY, VOL 75, 2021
(2021)
Review
Biochemistry & Molecular Biology
Julian Barth, Tim Schach, Jude M. Przyborski
Summary: Malaria parasites have a high metabolic rate and protein biosynthesis, encoding chaperone/heat shock proteins that play important roles in the parasite and host cells, making them potential targets for drug development.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Iga Dalidowska, Anna Orlowska, Marcin Smreczak, Pawel Bieganowski
Summary: The replication of Mononegavirales viruses relies on the chaperone activity of Hsp90, with a specific interaction between L and P proteins being necessary for functional viral polymerase. Interestingly, rabies and vesicular stomatitis virus L proteins can be expressed independently of P protein presence and in the presence of Hsp90 inhibitor.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Pavitra Prakash, Arpit Kumar Pradhan, Vasu Sheeba
Summary: Circadian disturbances are early features of neurodegenerative diseases, and this study found that neurotoxic modifiers Hsp40 and HSP70 can suppress circadian dysfunction. Overexpression of Hsp40 in Drosophila circadian ventrolateral neurons rescued the loss of circadian proteins and reduced the Huntingtin inclusion load. This suggests that molecular chaperones like Hsp40 play a role in circadian rehabilitation and may have broader therapeutic implications for neurodegenerative diseases.
DISEASE MODELS & MECHANISMS
(2022)
Article
Multidisciplinary Sciences
Hubert Wyszkowski, Anna Janta, Wiktoria Sztangierska, Igor Obuchowski, Tomasz Chamera, Agnieszka Klosowska, Krzysztof Liberek
Summary: The study investigates the functional differences between two classes of JDPs in yeast during the process of protein disaggregation, highlighting the superior aggregate binding ability of Ydj1 and the recruitment of more Ssa1 molecules to substrates by Sis1, which depends on its specific binding mechanism with Hsp70. These findings suggest that the subspecialization of JDPs during protein reactivation improves the robustness and efficiency of the disaggregation machinery.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Parasitology
Nancy A. Aguoru, Ruth S. Kirk, Anthony J. Walker
Summary: This study provides a detailed molecular bioinformatic analysis of heat shock proteins (HSPs) in the human parasitic blood fluke Schistosoma mansoni. The results reveal the classification, expression patterns, protein interactions, and phosphorylation sites of HSPs in different stages of the parasite's life cycle. The findings suggest that HSPs play important roles in parasite survival and development, and offer insights for future investigations and therapeutics development against schistosomiasis.
PARASITES & VECTORS
(2022)
Editorial Material
Cell Biology
Christopher M. Jakobson, Daniel F. Jarosz
Summary: Cells reorganize their contents into subcellular structures in response to stimuli, with new research showing that proteins interact with substrates to coordinate disassembly of cellular structures and directly sense the cellular energy state for adaptive reorganization.
NATURE CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sumera Zaib, Fatima Akram, Wania Waris, Syed Talha Liaqat, Zainab Zaib, Imtiaz Khan, Ayed A. Dera, Rami Adel Pashameah, Eman Alzahrani, Abd-ElAziem Farouk
Summary: This study explores potential inhibitors for the replication of dengue virus, using molecular docking, ADMET properties, and molecular dynamic simulation. By observing and validating interactions of various complexes, and conducting preclinical testing, a flavonoid called ladanein from Orthosiphon aristatus was identified as a lead compound with inhibitory potential against major replication protein of dengue virus.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Veterinary Sciences
Jae-Yeon Park, Jihoon Ryu, Jung-Eun Park, Eui-Ju Hong, Hyun-Jin Shin
Summary: This study found that HSP70 plays an important role in PEDV replication, with its overexpression increasing the speed of viral replication, while knockout significantly reduces PEDV protein expression and replication. Furthermore, the study confirmed the crucial role of the PEDV membrane (M) protein in collaboration with HSP70 in viral replication in infected cells.
VETERINARY RESEARCH
(2021)
