Review
Biochemistry & Molecular Biology
Louisa E. Wallace, Mengying Liu, Frank J. M. van Kuppeveld, Erik de Vries, Cornelis A. M. de Haan
Summary: Efficient penetration of the mucus layer is crucial for respiratory viruses to avoid mucociliary clearance before infection. Understanding the balance between receptor binding and destruction with host receptors is essential for efficient infection, especially when establishing in a new host species. The mucus layer is identified as a major determinant and barrier for zoonotic transfer due to its species-specific glycosylation patterns and dynamic interaction with respiratory viruses.
TRENDS IN MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Qiuchen Li, Zhimin Jiang, Shuning Ren, Hui Guo, Zhimin Song, Saini Chen, Xintao Gao, Fanfeng Meng, Junda Zhu, Litao Liu, Qi Tong, Honglei Sun, Yipeng Sun, Juan Pu, Kin-Chow Chang, Jinhua Liu
Summary: The induction of host serine and arginine-rich splicing factor 5 (SRSF5) by influenza A virus (IAV) enhances viral replication by promoting the splicing of viral M mRNA. SRSF5 directly interacts with M mRNA and U1 small nuclear ribonucleoprotein (snRNP) to facilitate M mRNA splicing and M2 protein production. Furthermore, the approved antifungal drug anidulafungin is identified as an inhibitor of SRSF5 and effectively blocks IAV replication.
Article
Virology
Yee-Chen Liu, Guan-Ru Liao, April Y. Tsai, Ching-Yu Tseng, Chih-Ying Kuan, Ruei-Sheng Tsai, Randy A. Albrecht, Rei-Lin Kuo, Ivan-Chen Cheng, Wan-Ting Liang, Shan-Chia Ou, Wei-Li Hsu
Summary: Influenza A virus H7N9, which crossed the avian-human barrier, causes severe disease in humans. The non-structural protein 1 (NS1) of H7N9 has a critical role in viral replication and virulence. Swapping the NS079 gene in recombinant avian influenza viruses resulted in attenuated replication, while replacing part of the NS079 effector domain with NS0702 sequence enhanced viral yield. Additionally, the NS2 protein was found to be an important determinant of infection efficiency.
Article
Microbiology
Tianxin Ma, Shiqi Niu, Zihua Wu, Shenghui Pan, Chenyang Wang, Xiaona Shi, Minghao Yan, Bangfeng Xu, Xingpo Liu, Luzhao Li, Dawei Yan, Qiaoyang Teng, Chunxiu Yuan, Xue Pan, Zhifei Zhang, Hoang Minh Duc, Zejun Li, Qinfang Liu
Summary: GNE is identified as a key host factor in the replication of influenza A virus, and its knockout significantly reduces the replication of multiple subtypes of influenza A virus in cells. It regulates the expression of sialic acid, the main receptor of the virus, and is involved in virus adsorption and endocytosis. This study provides novel insights into the replication mechanism of influenza A virus.
MICROBIOLOGY SPECTRUM
(2023)
Article
Cell Biology
Chenyang Yi, Cong Cai, Ze Cheng, Yifan Zhao, Xu Yang, Yue Wu, Xiaoping Wang, Zehua Jin, Yaozu Xiang, Meilin Jin, Li Han, Anding Zhang
Summary: This study identifies CYTH2 as a critical host gene for efficient influenza virus infection and suggests CYTH2 as an attractive target for developing host-directed antiviral drugs and therapeutics against influenza virus infection.
Article
Microbiology
Joseph M. Dybas, Krystal K. Lum, Katarzyna Kulej, Emigdio D. Reyes, Richard Lauman, Matthew Charman, Caitlin E. Purman, Robert T. Steinbock, Nicholas Grams, Alexander M. Price, Lydia Mendoza, Benjamin A. Garcia, Matthew D. Weitzman
Summary: Viral infections cause extensive remodeling of the cellular proteome. This study developed a global proteomics approach to analyze changes in the host proteome during adenovirus serotype 5 (Ad5) virus infection. The results reveal cellular processes manipulated by viral products and host factors counteracted by early viral proteins during infection.
Article
Multidisciplinary Sciences
Beryl Mazel-Sanchez, Justyna Iwaszkiewicz, Joao P. P. Bonifacio, Filo Silva, Chengyue Niu, Shirin Strohmeier, Davide Eletto, Florian Krammer, Gene Tan, Vincent Zoete, Benjamin G. Hale, Mirco Schmolke
Summary: The excessive production of viral glycoproteins during infections can put a significant stress on the host cell's endoplasmic reticulum protein folding machinery. However, viruses have developed mechanisms to optimize this stress for their own replication by balancing folding capacity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Virology
Thu Phan, Elizabeth J. Fay, Zion Lee, Stephanie Aron, Wei-Shou Hu, Ryan A. Langlois
Summary: The study introduces a new method InVERT, capable of quantifying all three RNA species produced by Influenza A virus, revealing the replication kinetics of different viral genome segments. The research further supports the complex regulation of RNA synthesis during replication, advancing our understanding of Influenza A virus replication dynamics.
JOURNAL OF VIROLOGY
(2021)
Article
Medicine, Research & Experimental
Yuchong Wang, Xujun Zhang, Kefan Bi, Hongyan Diao
Summary: MicroRNAs play important roles in regulating virus replication and host antiviral response during H1N1 infection, with some defending against the virus and others promoting viral replication. They are involved in host-viral interactions and have versatile functions in this process.
Article
Virology
Yasuha Arai, Norihito Kawashita, Emad Mohamed Elgendy, Madiha Salah Ibrahim, Tomo Daidoji, Takao Ono, Tatsuya Takagi, Takaaki Nakaya, Kazuhiko Matsumoto, Yohei Watanabe
Summary: This study identified novel clade 2.2.1 virus polymerase mutations that increased viral replication in human cells by analyzing phylogeny-associated PA mutations. These mutations, along with the PB2-E627K substitution, enhanced replication in contemporary clade 2.2.1.2 viruses compared to ancestral clade 2.2.1 viruses, indicating a higher public health risk for humans.
JOURNAL OF VIROLOGY
(2021)
Article
Microbiology
H-Heinrich Hoffmann, William M. Schneider, Francisco J. Sanchez-Rivera, Joseph M. Luna, Alison W. Ashbrook, Yadira M. Soto-Feliciano, Andrew A. Leal, Jeremie Le Pen, Inna Ricardo-Lax, Eleftherios Michailidis, Yuan Hao, Ansgar F. Stenzel, Avery Peace, C. David Allis, Scott W. Lowe, Margaret R. MacDonald, John T. Poirier, Charles M. Rice, Johannes Zuber
Summary: A study utilizing CRISPR-Cas9 technology targeted 332 members of the SARS-CoV-2 protein interactome, revealing new insights about coronaviruses and providing important references for future outbreaks.
CELL HOST & MICROBE
(2021)
Review
Biochemistry & Molecular Biology
Ludmila V. Puchkova, Irina V. Kiseleva, Elena V. Polishchuk, Massimo Broggini, Ekaterina Yu. Ilyechova
Summary: Three main approaches to combat severe viral respiratory infections include preemptive vaccination, chemical agents inhibition of viral proteins, and targeting host metabolism. The challenge lies in the evolving nature of the viral genome which impacts the effectiveness of vaccines and drugs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Ling Bu, Boqian Chen, Lei Xing, Xuejun Cai, Shuhua Liang, Liying Zhang, Xinhua Wang, Wenjun Song
Summary: The study successfully generated a recombinant pdmH1N1 2009 IAV with mCherry gene marker through serial passage in mice, demonstrating comparable growth kinetics to the wild-type. The IAV reporter virus is a powerful tool for studying viral pathogenicity and transmission, as well as developing and evaluating novel anti-viral drugs, inhibitors, and vaccines in the future.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Ao Zhou, Xia Dong, Mengyun Liu, Bin Tang
Summary: The study systematically analyzed mRNA expression profiles of host genes in different human cells infected with various subtypes of IAV to better understand host responses to IAV and develop broad-spectrum antiviral drugs. The results showed significant differences in the expression of host genes, especially those involved in immune defense and antiviral response, with key antiviral factors identified in different cell types. Overlaps of upregulated genes by IAV in different cell types may serve as early detection markers or broad-spectrum antiviral targets.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiaonan Liu, Sini Huuskonen, Tuomo Laitinen, Taras Redchuk, Mariia Bogacheva, Kari Salokas, Ina Pohner, Tiina Ohman, Arun Kumar Tonduru, Antti Hassinen, Lisa Gawriyski, Salla Keskitalo, Maria K. Vartiainen, Vilja Pietiainen, Antti Poso, Markku Varjosalo
Summary: Research on SARS-CoV-2-host interactions has identified hub proteins that could be valuable for drug repurposing, leading to the potential repurposing of 59 compounds for 15 protein targets. In vitro validation also demonstrated the antiviral effects of some candidate drugs, suggesting possible therapeutic options for COVID-19.
MOLECULAR SYSTEMS BIOLOGY
(2021)
Article
Allergy
Sascha Hein, Marie-Luise Herrlein, Ines Mhedhbi, Daniela Bender, Vanessa Haberger, Nuka Benz, Jonathan Eisert, Julia Stingl, Michael Dreher, Doris Oberle, Jessica Schulze, Christin Mache, Matthias Budt, Christoph Hildt, Thorsten Wolff, Eberhard Hildt
Summary: The study revealed differences in titer, neutralizing capacity, and affinity of antibodies between sera elicited by BNT162b2 and CVnCoV vaccines, which could potentially contribute to the observed differences in vaccine efficacy. BNT162b2-elicited sera and convalescent sera showed higher levels of spike-RBD-specific antibodies and neutralizing antibodies compared to CVnCoV-elicited sera, with a reduction in binding and neutralizing antibodies for the B.1.351 variant of concern.
Review
Cell Biology
Erik McShane, Matthias Selbach
Summary: Cellular protein degradation is an active and highly regulated process that has diverse roles in biological functions, including homeostasis, regulation, and quality control.
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Immunology
Yikun Yao, Ping Du Jiang, Brittany N. Chao, Deniz Cagdas, Satoshi Kubo, Arasu Balasubramaniyam, Yu Zhang, Bella Shadur, Adeeb NaserEddin, Les R. Folio, Benjamin Schwarz, Eric Bohrnsen, Lixin Zheng, Matthew Lynberg, Simone Gottlieb, Michael A. Leney-Greene, Ann Y. Park, Ilhan Tezcan, Ali Akdogan, Rahsan Gocmen, Sevgen Onder, Avi Rosenberg, Elizabeth J. Soilleux, Errin Johnson, Peter K. Jackson, Janos Demeter, Samuel D. Chauvin, Florian Paul, Matthias Selbach, Haydar Bulut, Menna R. Clatworthy, Zewen K. Tuong, Hanlin Zhang, Benjamin J. Stewart, Catharine M. Bosio, Polina Stepensky, Simon Clare, Sundar Ganesan, John C. Pascall, Oliver Daumke, Geoffrey W. Butcher, Andrew J. McMichael, Anna Katharina Simon, Michael J. Lenardo
Summary: This study describes a new inborn error of immunity caused by mutations in the GTPase of the immune-associated protein 6 (GIMAP6) gene. This condition leads to infections, lymphoproliferation, autoimmunity, and multiorgan vasculitis in affected patients. The study shows that GIMAP6 is involved in autophagy, redox regulation, and polyunsaturated fatty acid (PUFA)-containing lipids. Additionally, GIMAP6 interacts with GABARAPL2 and GIMAP7 to regulate GTPase activity. Furthermore, GIMAP6 is induced by IFN-gamma and plays a critical role in antibacterial immunity. The study also finds that Gimap6(-/-) mice die prematurely due to GIMAP6 deficiency in kidney endothelial cells.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Article
Biochemical Research Methods
Tommaso Mari, Kirstin Moesbauer, Emanuel Wyler, Markus Landthaler, Christian Drosten, Matthias Selbach
Summary: Phosphoproteomics quantifies phosphorylation site changes, but functional analysis is challenging. A new database, iKiP-DB, accurately predicts kinase activity changes and is applied in a SARS-CoV-2 study.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Carlos Henrique Vieira-Vieira, Vita Dauksaite, Anje Sporbert, Michael Gotthardt, Matthias Selbach
Summary: Researchers have developed a quantitative RNA-interactome capture method to assess the function of phosphorylation sites in RNA-binding proteins.
Article
Multidisciplinary Sciences
Ulrike Zinnall, Miha Milek, Igor Minia, Carlos H. Vieira-Vieira, Simon Mueller, Guido Mastrobuoni, Orsalia-Georgia Hazapis, Simone Del Giudice, David Schwefel, Nadine Bley, Franka Voigt, Jeffrey A. Chao, Stefan Kempa, Stefan Huettelmaier, Matthias Selbach, Markus Landthaler
Summary: HDLBP/Vigilin directly interacts with more than 80% of ER-localized mRNAs and plays an important role in the translation of ER-targeted mRNAs. Its absence leads to impaired protein synthesis and secretion, as well as decreased tumor growth.
NATURE COMMUNICATIONS
(2022)
Article
Cell & Tissue Engineering
Ann-Kathrin Fanti, Katrin Busch, Alessandro Greco, Xi Wang, Branko Cirovic, Fuwei Shang, Tamar Nizharadze, Larissa Frank, Melania Barile, Thorsten B. Feyerabend, Thomas Hoefer, Hans-Reimer Rodewald
Summary: In response to infections and stress, hematopoiesis rapidly enhances blood and immune cell production. The specific stage responsible for this regeneration was unclear, but experiments on mice showed that hematopoietic stem cells (HSCs) and multipotent progenitors (MPPs) played different roles. HSCs responded to sepsis by increasing proliferation and cell death, while MPPs were identified as a major source for accelerated myeloid cell production. This suggests that progenitors, rather than stem cells, regulate blood and immune cell homeostasis to ensure a rapid response and maintain the integrity of the HSC population.
Article
Biochemistry & Molecular Biology
Koshi Imami, Matthias Selbach, Yasushi Ishihama
Summary: In this study, we introduced a mass spectrometry-based proteomic method that combines biochemical isolation of mitochondria with pulse stable isotope labeling by amino acids in cell culture, which provides the highest protein identification rate with the shortest measurement time among currently available methods, enabling the quantification of 12 of the 13 mitochondrial-encoded proteins. This method was applied to uncover the global picture of (post-)translational regulation of both mitochondrial- and nuclear-encoded subunits of oxidative phosphorylation complexes.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Endocrinology & Metabolism
Ming Gao, Minjian Chen, Qiuzhen Chen, Shuai Zhu, Hengjie Wang, Weizheng Yang, Xi Wang, Qiang Wang, Ling Gu
Summary: Well-controlled metabolism is crucial for optimal oocyte development. This study investigated the intrinsic regulation of oocyte maturation through metabolomics and transcriptomics analysis, revealing novel metabolic features during porcine oocyte meiosis. The findings provide a comprehensive data resource and potential molecular biomarkers for predicting and improving oocyte quality.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Bo Qin, Simon M. M. Lauer, Annika Balke, Carlos H. H. Vieira-Vieira, Jorg Buerger, Thorsten Mielke, Matthias Selbach, Patrick Scheerer, Christian M. T. Spahn, Rainer Nikolay
Summary: Ribosome biogenesis is a multi-step cellular process that involves the production, processing, folding, and modification of ribosomal RNAs (rRNAs) and ribosomal proteins. By using a minimal in vitro reconstitution system, researchers studied the early assembly phase of the bacterial 50S subunit and found that it occurs in a domain-wise fashion, with late assembly proceeding incrementally. The study also showed that ribosomal proteins and folded rRNA helices induce or stabilize rRNA folds within adjacent regions, creating cooperativity.
NATURE COMMUNICATIONS
(2023)
Article
Pharmacology & Pharmacy
Georgios Vavouras Syrigos, Maximilian Feige, Alicia Dirlam, Ramona Businger, Iris Gruska, Luder Wiebusch, Klaus Hamprecht, Michael Schindler
Summary: Human cytomegalovirus (HCMV) is a dangerous virus that causes serious infections in certain groups of people. It interacts with cellular kinases and encodes a viral kinase called pUL97, which phosphorylates an antiviral factor called SAMHD1. Researchers discovered that CDK inhibitors (CDKIs), drugs approved by the FDA, can inhibit the phosphorylation of SAMHD1 and effectively inhibit HCMV replication in macrophages. This study highlights the potential of CDKIs as antiviral drugs against HCMV and establishes SAMHD1 as a therapeutic target.
ANTIVIRAL RESEARCH
(2023)
Article
Immunology
Nina Weisshaar, Sicong Ma, Yanan Ming, Alaa Madi, Alessa Mieg, Marvin Hering, Ferdinand Zettl, Kerstin Mohr, Nora Ten Bosch, Diana Stichling, Michael Buettner, Gernot Poschet, Glynis Klinke, Michael Schulz, Nina Kunze-Rohrbach, Carolin Kerber, Isabel Madeleine Klein, Jingxia Wu, Xi Wang, Guoliang Cui
Summary: The main function of the malate shuttle in CD8+ T cells is to detoxify ammonia and enable sustainable ammonia-neutral glutamine catabolism during chronic infection, rather than maintaining the NAD+/NADH balance.
Article
Microbiology
Boris Bogdanow, Iris Gruska, Lars Muehlberg, Jonas Protze, Svea Hohensee, Barbara Vetter, Jens B. Bosse, Martin Lehmann, Mohsen Sadeghi, Lueder Wiebusch, Fan Liu
Summary: Using crosslinking mass spectrometry and quantitative proteomics, we identified the spatially resolved interactome map of intact human cytomegalovirus virions, discovering the localization of viral and host proteins within the multilayered tegument. The viral protein UL32 plays a critical role in bridging the nucleocapsid to the viral envelope, while also antagonizing the binding of 14-3-3 proteins through recruitment of PP-1 phosphatase. Our coarse-grained model provides global insights into the native configuration of virus and host protein interactions within herpesvirions.
NATURE MICROBIOLOGY
(2023)
Article
Medicine, Research & Experimental
Clemens Cammann, Jonas Kulla, Christian Walz, Fang Zhao, Theresa Lowinus, Eylin Topfstedt, Neha Mishra, Petra Henklein, Ursula Bommhardt, Lukas Bossaller, Christian Hagemeier, Dirk Schadendorf, Boris Schmidt, Annette Paschen, Ulrike Seifert
Summary: The combination of proteasome and Kv1.3 potassium channel inhibitors can inhibit the growth of BRAF-mutant melanoma cells and induce apoptosis. This combination therapy may serve as an alternative approach to treat other tumors expressing mutated BRAF.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Oncology
Hamed Alborzinia, Andres F. Florez, Sina Kreth, Lena M. Brueckner, Umut Yildiz, Moritz Gartlgruber, Dorett Odoni, Gernot Poschet, Karolina Garbowicz, Chunxuan Shao, Corinna Klein, Jasmin Meier, Petra Zeisberger, Michal Nadler-Holly, Matthias Ziehm, Franziska Paul, Juergen Burhenne, Emma Bell, Marjan Shaikhkarami, Roberto Wuerth, Sabine A. Stainczyk, Elisa M. Wecht, Jochen Kreth, Michael Buettner, Naveed Ishaque, Matthias Schlesner, Barbara Nicke, Carlo Stresemann, Maria Llamazares-Prada, Jan H. Reiling, Matthias Fischer, Ido Amit, Matthias Selbach, Carl Herrmann, Stefan Woelfl, Kai-Oliver Henrich, Thomas Hoefer, Andreas Trumpp, Frank Westermann
Summary: The study reveals that MYCN-amplified neuroblastoma undergoes ferroptosis in the absence of cysteine, suggesting a potential therapeutic approach of combining cysteine depletion and GPX4 inactivation. In addition, the study provides evidence that targeting multiple ferroptosis targets can be a promising therapeutic strategy for aggressive MYCN-amplified tumors.