Article
Immunology
Zuoji Ye, Liumei Ye, Dingbin Li, Shunsheng Lin, Wusheng Deng, Li Zhang, Jinhua Liang, Jinlong Li, Qingjun Wei, Ke Wang
Summary: In this study, the researchers found that Daphnetin (DAP) can effectively inhibit and eradicate biofilm formation of Pseudomonas aeruginosa. DAP also reduced bacterial motility and pyocyanin production. These findings suggest that DAP may serve as a natural anti-biofilm therapeutic agent.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Ahmed A. Abdelaziz, Amal M. Abo Kamer, Khaled B. Al-Monofy, Lamiaa A. Al-Madboly
Summary: The discussion focuses on the bioprospecting of microorganisms and their bioactive byproducts, specifically pigments. Microbial pigments, such as pyocyanin produced by Pseudomonas aeruginosa, have various benefits including their natural composition, therapeutic effects, and year-round production. This article concentrates on the production, extraction, and biological applications of pyocyanin pigment in biotechnology, engineering, and biology fields.
MICROBIAL CELL FACTORIES
(2023)
Article
Microbiology
Theerthankar Das, Biswanath Das, Brandon Clark Young, Vina Aldilla, Shekh Sabir, Basmah Almohaywi, Mark Willcox, Mike Manefield, Naresh Kumar
Summary: The presence of ascorbic acid can interact with pyocyanin, resulting in shifts in its absorbance peaks and structure. This interaction impairs the binding of pyocyanin to DNA and disrupts biofilm formation. In combination with furanone-30, ascorbic acid further inhibits quorum sensing and enhances antibiotic-mediated bacterial killing, reducing the virulence and biofilm formation of Pseudomonas aeruginosa. The antioxidant properties of ascorbic acid play a crucial role in these interactions and their effects.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Adilson Jose da Silva, Josivan de Souza Cunha, Teri Hreha, Kelli Cristina Micocci, Heloisa Sobreiro Selistre-de-Araujo, Blanca Barquera, Mattheos A. G. Koffas
Summary: This study engineered the pyocyanin biosynthetic pathway for high production in a heterologous host for the first time. Optimized culture and induction parameters led to a 3.5-fold increase in pyocyanin accumulation, while pathway balancing through testing plasmids with different copy numbers resulted in a fourfold difference in product titer among the engineered strains. Co-expression of Vitreoscilla hemoglobin Vhb further improved pyocyanin production to a final titer of 18.8 mg/L, demonstrating the potential of using Escherichia coli for phenazines production in electro-fermentation systems.
METABOLIC ENGINEERING
(2021)
Article
Immunology
Andi Suo, Zhebin Hua, Caie Wu, Gongjian Fan, Tingting Li, Kaiping Cong
Summary: This study investigated the bacterial inhibitory activity of ginkgolic acid (GA) and evaluated the potential of ginkgo biloba exocarp as a quorum sensing inhibitor. The results showed that GA could inhibit the formation of quorum sensing dependent virulence factors but promoted biofilm formation. The experimental results were verified and suggested that GA has potential as an anti-virulence factor drug.
MICROBIAL PATHOGENESIS
(2022)
Article
Immunology
Andi Suo, Zhebin Hua, Caie Wu, Gongjian Fan, Tingting Li, Kaiping Cong
Summary: This study investigated the quorum sensing activity of ginkgolic acid (GA) and found that it was able to inhibit the formation of quorum sensing dependent virulence factors in Pseudomonas aeruginosa PAO1, but promoted the formation of biofilms. These results suggest that GA has the potential as an anti-virulence factor drug, but is not suitable for development as a broad-spectrum biofilm inhibitor.
MICROBIAL PATHOGENESIS
(2022)
Article
Biochemistry & Molecular Biology
Syed Ghazanfar Ali, Mohammad Jalal, Hilal Ahmad, Khalid Umar, Akil Ahmad, Mohammed B. B. Alshammari, Haris Manzoor Khan
Summary: Antimicrobial resistance is a serious global health concern due to excessive use of antibiotics. In this study, gold nanoparticles synthesized from the plant Tinospora cordifolia were tested against multidrug-resistant Pseudomonas aeruginosa, showing effective antimicrobial properties.
Article
Infectious Diseases
Rosanna Papa, Gianluca Vrenna, Caterina D'Angelo, Angela Casillo, Michela Relucenti, Orlando Donfrancesco, Maria Michela Corsaro, Ersilia Vita Fiscarelli, Vanessa Tuccio Guarna Assanti, Maria Luisa Tutino, Ermenegilda Parrilli, Marco Artini, Laura Selan
Summary: The study demonstrated that supernatant from an Antarctic bacterium can inhibit specific virulence factors produced by Pseudomonas aeruginosa strains associated with cystic fibrosis patients, including reduced pyocyanin and protease production, as well as impacting swarming and swimming motility. This indicates that polar bacteria may be potential candidates for discovering new compounds that limit P. aeruginosa virulence in CF patients.
Article
Biotechnology & Applied Microbiology
Obinna Markraphael Ajunwa, Olubusola Ayoola Odeniyi, Emmanuel Oluwaseun Garuba, Mrinalini Nair, Enrico Marsili, Abiodun Anthony Onilude
Summary: This study determined the production of pyocyanin, NAD, and NAD synthetase in the electrogenic bacterium Pseudomonas aeruginosa using a Microbial Fuel Cell (MFC). The co-expression of nadD and nadC genes increased the activity of NAD synthetase and resulted in higher pyocyanin concentration, NAD/NADH levels, and MFC potential in PA-A4 strain. This finding advances our understanding of NAD biosynthetic genes in PA electrogenicity.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Microbiology
Fernanda Jimenez Otero, Dianne K. Newman, Leonard M. Tender
Summary: Biofilms provide a protective environment but also present challenges to the cells living within them. Pseudomonas aeruginosa overcomes oxygen limitation by secreting redox active phenazines, which act as electron shuttles. Blocking the re-oxidation of one of these electron shuttles, pyocyanin, decreases cell survival within biofilms and enhances the effect of gentamicin in killing cells. The study highlights the importance of redox cycling of electron shuttles in P. aeruginosa biofilms.
Review
Biochemistry & Molecular Biology
Maria Sultan, Rekha Arya, Kyeong Kyu Kim
Summary: Pseudomonas aeruginosa is an opportunistic pathogen with potential threat to human health worldwide due to its high virulence and significant resistance to multiple antibiotics. Understanding the mechanism of two-component systems in controlling virulence factors during infection is crucial for developing new antibiotics to treat diseases caused by P. aeruginosa.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Marco Artini, Gianluca Vrenna, Marika Trecca, Vanessa Tuccio Guarna Assanti, Ersilia Vita Fiscarelli, Rosanna Papa, Laura Selan
Summary: Pseudomonas aeruginosa is a bacterium frequently involved in cystic fibrosis airway infections. It increases its virulence through biofilm, motility, toxin production, and invasion of host cells. The metalloprotease serratiopeptidase (SPEP) has been shown to impair the virulence-related properties of Gram-positive bacteria. In this study, the effects of SPEP on different physiological aspects related to the virulence of P. aeruginosa isolated from CF patients were investigated. The results showed that SPEP impaired attachment to inert surfaces and adhesion/invasion of eukaryotic cells. It also had strain-dependent effects on pyocyanin and pyoverdine production. Additionally, SPEP seemed to increase swarming motility and staphylolytic protease production. Further studies are needed to understand why different strains react differently to specific treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Gianluca Vrenna, Marco Artini, Rino Ragno, Michela Relucenti, Ersilia Vita Fiscarelli, Vanessa Tuccio Guarna Assanti, Rosanna Papa, Laura Selan
Summary: The study found that Coridothymus capitatus essential oil (CCEO) can inhibit biofilm formation and toxin production by Pseudomonas aeruginosa, showing significant effects on most tested strains. CCEO also has the ability to dramatically alter the extracellular matrix structure and impact the motility of P. aeruginosa.
Article
Biotechnology & Applied Microbiology
Heba Shouman, Heba Shehta Said, Hany I. Kenawy, Ramadan Hassan
Summary: This study aims to screen and produce pyocyanin from clinical and environmental P. aeruginosa isolates, and evaluate its biological activity. The purified pyocyanin showed remarkable antimicrobial efficacy, biofilm inhibition and eradication, as well as antioxidant and cytotoxic activity against cancer cell lines with minimal cytotoxicity to normal cells.
MICROBIAL CELL FACTORIES
(2023)
Article
Multidisciplinary Sciences
Chelsey M. VanDrisse, Rosalie Lipsh-Sokolik, Olga Khersonsky, Sarel J. Fleishman, Dianne K. Newman
Summary: This study demonstrates that by engineering the structure of PodA protein and combining it with tobramycin, the killing effect on P. aeruginosa can be enhanced, especially on biofilms that are resistant to conventional antibiotic treatment.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Public, Environmental & Occupational Health
Fadilah S. Aleanizy, Fulwah Y. Alqahtani, Marzouqah S. Alanazi, Rania A. E. H. Mohamed, Bahauddeen M. Alrfaei, Mana M. Alshehri, Hajar AlQahtani, Ghalia Shamlan, Nassr Al-Maflehi, Maha M. Alrasheed, Ahmed Alrashed
Summary: This study aimed to describe clinical and demographic features of COVID-19 patients, and found that old age, fever, and comorbidities involving diabetes mellitus, asthma, and smoking were significantly associated with infection severity.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2021)
Article
Multidisciplinary Sciences
Rania Ali El Hadi Mohamed, Fadilah Sfouq Aleanizy, Fulwah Y. Alqahtani, Eman A. Alhmoaidi, Nahla Mohamed
Summary: This study screened wild shrew specimens in Saudi Arabia for RNA viruses, finding multiple viruses propagating in the tissues of the shrews. This first documentation of the viruses in the country highlights the neglected wild reservoirs of arboviruses and suggests future studies should evaluate other potential hosts, including bats.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2021)
Review
Chemistry, Multidisciplinary
Fakhria A. Al-Joufi, Aseem Setia, Mounir M. Salem-Bekhit, Ram Kumar Sahu, Fulwah Y. Alqahtani, Retno Widyowati, Fadilah Sfouq Aleanizy
Summary: This article addresses the molecular pathogenesis of colorectal cancer (CRC) and highlights the potential of biomarkers for predicting disease behavior and prognosis. It also provides conceptual knowledge of nanotechnology-based diagnostic techniques and therapeutic approaches for malignant CRC.
Article
Public, Environmental & Occupational Health
Hajar AlQahtani, Sara AlBilal, Ebrahim Mahmoud, Omar Aldibasi, Ahmad Alharbi, Nour Shamas, Abdulrahman Alsaedy, Kenana Owaidah, Fulwah Yahya Alqahtani, Fadilah Sfouq Aleanizy, Hatim Arishi, Saleem Baharoon, Mohammad Bosaeed
Summary: This study found that age 60 and above is the only factor associated with higher mortality rate in COVID-19 patients, regardless of the type of immunomodulator therapy. There is a lack of synergistic effect between TCZ and DEX on hospital mortality.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2022)
Article
Pharmacology & Pharmacy
Jouri S. Alanazi, Fulwah Yahya Alqahtani, Fadilah Sfouq Aleanizy, Awwad A. Radwan, Ahmed Bari, Qamraa Hamad Alqahtani, Hosam Gharib Abdelhady, Ibrahim Alsarra
Summary: This study aims to develop a new treatment modality for choroidal neovascularization (CNV) by utilizing targeted nanoparticles to deliver microRNA-539-5p (miR-539). The researchers found that miR-539 incorporated in nanoparticles reduced the viability of human retinal microvascular endothelial cells (HRMECs). Furthermore, functionalizing the nanoparticles with a specific targeting peptide enhanced the uptake of the nanoparticles.
Article
Nanoscience & Nanotechnology
Hosam Gharib Abdelhady, Fadilah Sfouq Aleanizy, Fulwah Yahya Alqahtani, Hamad Alkahtani
Summary: The effect of core length on the physicochemical properties and purity of PAMAM dendrimers was studied. Increasing the core length significantly changed the morphology of the dendrimers and improved their purity.
JOURNAL OF NANOMATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Ahad S. Abushal, Fadilah S. Aleanizy, Fulwah Y. Alqahtani, Faiyaz Shakeel, Muzaffar Iqbal, Nazrul Haq, Ibrahim A. Alsarra
Summary: Psoriatic arthritis is an autoimmune disease that causes joint inflammation and damage. The current treatments are limited in effectiveness and convenience. A study found that a self-nanoemulsifying drug delivery system (SNEDDS) improved the solubility and bioavailability of the medication Apremilast. The optimized formulation, F9, showed the highest drug release in vitro and further investigation is needed for its potential as an alternative delivery system.
Article
Pharmacology & Pharmacy
Esra Kamal Eltayb, Fadilah Sfouq Aleanizy, Fulwah Y. Alqahtani, Hamad M. Alkahtani, Siddique Akber Ansari, Ibrahim Alsarra
Summary: This study successfully prepared calcium alginate nanoparticles (CANPs) loaded with mBTL, a quorum and virulence inhibitor for Pseudomonas aeruginosa. The synthesized CANPs showed stable nano-size particles and sustained drug release profile. The mBTL-loaded-CANPs displayed stable structure and no interaction between mBTL and polymer. The findings suggest the potential of mBTL-loaded-CANPs in alleviating infections caused by Pseudomonas aeruginosa.
SAUDI PHARMACEUTICAL JOURNAL
(2022)
Article
Endocrinology & Metabolism
Fulwah Yahya Alqahtani, Fadilah Sfouq Aleanizy, Rania Ali El Hadi Mohamed, Nassr Al-Maflehi, Bahauddeen M. Alrfaei, Nora Alkhudair, Thamer A. Almangour, Ghada Bawazeer, Ghalia Shamlan, Marzouqah S. Alanazi
Summary: This study examined the association between different levels of body mass index (BMI) and the severe form of COVID-19. It found that patients with a BMI >40 kg/m2 had a higher risk of severe COVID-19 compared to those with normal weight.
DIABETES METABOLIC SYNDROME AND OBESITY-TARGETS AND THERAPY
(2022)
Correction
Multidisciplinary Sciences
Rania Ali El Hadi Mohamed, Fadilah Sfouq Aleanizy, Fulwah Y. Alqahtani, Eman A. Alhmoaidi, Nahla Mohamed
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Rawan Alswailem, Fulwah Yahya Alqahtani, Fadilah Sfouq Aleanizy, Bahauddeen M. Alrfaei, Mohammad Badran, Qamraa Hamad Alqahtani, Hosam Gharib Abdelhady, Ibrahim Alsarra
Summary: The use of miR-219 nanoparticles shows potential for the treatment of glioblastoma, as they have been found to induce apoptosis in GBM cells.
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Rehab A. Alshammari, Fadilah S. Aleanizy, Amal Aldarwesh, Fulwah Y. Alqahtani, Wael A. Mahdi, Bushra Alquadeib, Qamraa H. Alqahtani, Nazrul Haq, Faiyaz Shakeel, Hosam G. Abdelhady, Ibrahim A. Alsarra
Summary: This study developed RBX nanoparticles using PAMAM dendrimers for the treatment of diabetic retinopathy, providing a safe and effective method for RBX administration. The drug loading efficiency and in vitro release of the RBX:PAMAM dendrimer complexes were evaluated, and the 5:1 complex showed the highest loading efficiency and drug release. The nanoformulation improved the therapeutic outcomes of anti-VEGF therapy and the bioavailability of RBX for preventing vision loss in patients with diabetic retinopathy.
Article
Polymer Science
Esra Kamal Eltayb, Fulwah Yahya Alqahtani, Hamad M. Alkahtani, Ibrahim A. Alsarra, Rihaf Alfaraj, Fadilah Sfouq Aleanizy
Summary: Pseudomonas aeruginosa, a pathogen responsible for many chronic infections and resistant to multiple antibiotics, can be effectively targeted by meta-bromo-thiolactone (mBTL)-loaded calcium alginate nanoparticles (CANPs). These nanoparticles exhibit higher antibacterial and anti-virulence activity, reduce the biofilm formation and pyocyanin production of P. aeruginosa, and show low cytotoxic effects on human lung epithelial cells and cystic fibrosis fibroblast cells. It is suggested that mBTL-CANPs have great potential in prophylaxis and treatment of Pseudomonas aeruginosa infections.
Article
Infectious Diseases
Menyfah Q. Alanazi, Hajar AlQahtani, Thamer A. Almangour, Fadilah Sfouq Aleanizy, Fulwah Yahya Alqahtani
Summary: This study aimed to assess the prevalence and treatment patterns of respiratory tract infections (RTIs) in the emergency department (ED), including antibiotic-prescribing errors and the cost of inappropriate antibiotic use. The study found that the overall prevalence of RTIs in the ED was 21%, with 53% of antibiotic prescriptions being deemed inappropriate. There is a strong need to establish antimicrobial stewardship programs to improve antibiotic use in the ED.
Article
Biochemistry & Molecular Biology
Wafa K. Fatani, Fadilah S. Aleanizy, Fulwah Y. Alqahtani, Mohammed M. Alanazi, Abdullah A. Aldossari, Faiyaz Shakeel, Nazrul Haq, Hosam Abdelhady, Hamad M. Alkahtani, Ibrahim A. Alsarra
Summary: Lung cancer is the leading cause of cancer-related deaths worldwide. Erlotinib, a tyrosine kinase inhibitor, has been explored as a potential treatment for lung cancer. Researchers have formulated two different generations of polyamidoamine (PAMAM) dendrimers and evaluated their efficacy in delivering erlotinib. The results showed that the cationic G4 dendrimer had higher selectivity and antiproliferative activity against lung cancer cells compared to the neutral G5 dendrimer and erlotinib alone, suggesting its potential as a targeted and sustained release carrier for erlotinib.