Article
Biotechnology & Applied Microbiology
Ramzi Mukred Saeed, Mohammed Abdullah, Mamoun Ahram, Mutasem Omar Taha
Summary: The study developed novel ellipsoid non-cytotoxic NPs crosslinked with TPP, utilizing the advantages of CSP and LC to efficiently deliver siRNA into cancer cells. The NPs showed excellent stability and cellular penetration, achieving significant silencing efficiency against SLUG protein.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Saeem Ahmad, Iram Khan, Jayamanti Pandit, Nasr A. Emad, Shahnaj Bano, Khalid Imtiyaz Dar, M. Moshahid Alam Rizvi, Mohd Danish Ansari, Mohd. Aqil, Yasmin Sultana
Summary: This study successfully developed chitosan-coated PLGA nanoparticles for nose-to-brain delivery of carmustine. The optimized nanoparticles showed satisfactory levels in terms of particle size, entrapment efficiency, drug release, and permeability. The results suggested that chitosan-coated nanoparticles could be a promising approach for delivering carmustine to treat Glioblastoma multiforme via intranasal administration.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Medicine, Research & Experimental
Meihua Luo, Yuchen Li, Bo Peng, Jacinta White, Ermei Makila, Wing Yin Tong, Chung Hang Jonathan Choi, Bryan Day, Nicolas H. Voelcker
Summary: The clinical treatment of glioblastoma (GBM) is challenging due to the blood-brain barrier, chemotherapeutic resistance, and aggressive tumor metastasis. In this study, researchers developed porous silicon nanoparticles (pSiNPs) modified with antisense oligonucleotide (AON) for targeted gene and drug delivery in GBM treatment. These AON-modified pSiNPs (AON@pSiNPs) had selective internalization by GBM cells and human cerebral microvascular endothelial cells expressing scavenger receptors. The results showed that AON@pSiNPs could cross the blood-brain barrier, knock down the key driver of GBM invasion, and inhibit GBM cell migration, making them a potential strategy for clinical treatment of GBM and targeted drug and gene delivery.
MOLECULAR PHARMACEUTICS
(2023)
Article
Biophysics
Naghmeh Akbari Moghadam, Fatemeh Bagheri, Mohamadreza Baghaban Eslaminejad
Summary: Chitosan-chondroitin sulfate nanoparticles have been synthesized as a platform for efficient gene sequence delivery, showing potential for gene therapy in cartilage diseases.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Review
Biochemistry & Molecular Biology
Xudong Li, Yuran Wang, Chenyun Feng, Haijun Chen, Yu Gao
Summary: This review discusses the application of chitosan in cancer theranostics, including its chemical functionalities, synthesis methods, and the improvement of treatment efficiency through functionalization. Several case studies of chitosan-based nanotheranostics are also reported.
Article
Polymer Science
Ali Dehshahri, Bahman Khalvati, Zahra Taheri, Farshad Safari, Reza Mohammadinejad, Abolfazl Heydari
Summary: This study evaluated the transfection efficiency and cytotoxicity of N-[(2-hydroxy-3-trimethylammonium)propyl] chitosan salt (HTCS) as a gene carrier. HTCS was synthesized and used for the delivery of the interleukin-12 (IL-12) gene plasmid. The results showed that HTCS could effectively condense the IL-12 plasmid into polyplexes and demonstrated higher hIL-12 production compared to CS and polyethylenimine polyplexes in cell transfection experiments.
Article
Biochemistry & Molecular Biology
Jens Casper, Laura Nicolle, Melanie Willimann, Evrim Umit Kuzucu, Alan Tran, Perrine Robin, Pascal Detampel, Hiu Man Grisch-Chan, Beat Thony, Jorg Huwyler, Sandrine Gerber-Lemaire
Summary: The study investigates the improvement of nucleic acid delivery by using depolymerized chitosan-polyethylenimine DNA complexes. This modular approach combines core-dCS-linear PEI/DNA complexes with a shell consisting of dCS-PEG-COOH, resulting in improved nucleic acid encapsulation, cellular uptake, and transfection potency. The optimized ternary complex shows high reporter gene signal for three days after administration, indicating its potential for in vitro and in vivo gene delivery.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Pharmacology & Pharmacy
Afsana Sheikh, Shadab Md, Nabil A. Alhakamy, Prashant Kesharwani
Summary: There is a growing demand for safe and targeted cancer therapies as conventional treatments often fail to reach the intended site. Nanocarriers enable targeted delivery, increasing drug concentration at the tumor site while reducing toxicity to healthy cells and overcoming drug resistance. Aptamers, with their ability to recognize and interact with biomarkers overexpressed by cancer cells, play a crucial role in cancer immunotherapy and drug delivery. This review highlights the use of aptamer-chitosan nanoparticles in cancer therapy and their potential for reducing toxic effects.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Review
Polymer Science
Mahwash Mukhtar, Eszter Fenyes, Csilla Bartos, Mahira Zeeshan, Rita Ambrus
Summary: Chitosan, a natural biopolymer, is commonly used in drug delivery due to its biodegradability and biocompatibility. Its derivatives have been synthesized to enhance mucoadhesion and solubility, while nano and micro particles of chitosan have been reported to improve drug bioavailability. Chitosan has also shown promise in active targeted drug and gene delivery, thanks to its tunable surface modifications.
EUROPEAN POLYMER JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Panpan Song, Nannan Song, Li Li, Minghao Wu, Zhongxia Lu, Xia Zhao
Summary: A pH/reduction-sensitive carboxymethyl chitosan nanogel modified by targeting peptide angiopep-2 loaded with doxorubicin shows promising potential for targeted chemotherapy of glioblastoma. The targeted nanogel exhibited good stability, biocompatibility, and enhanced BBB penetration and tumor targeting ability, leading to higher drug uptake and stronger antitumor activity compared to nontargeted nanogels. This innovative nanogel platform with pH/reduction dual-stimuli response may hold great significance for GBM-targeted therapy.
Article
Biochemistry & Molecular Biology
Muhammad Babar Khan, Rosamaria Ruggieri, Eesha Jamil, Nhan L. Tran, Camila Gonzalez, Nancy Mugridge, Steven Gao, Jennifer MacDiarmid, Himanshu Brahmbhatt, Jann N. Sarkaria, John Boockvar, Marc Symons
Summary: The study suggests that microRNA-34a can effectively reduce the activation status of glioblastoma cells and enhance survival rates against multiple glioblastoma cell types. Combination therapy of temozolomide with microRNA-34a loaded nanocells targeted to epidermal growth factor receptor (EGFR) significantly enhances sensitivity of glioblastoma cells to treatment.
MOLECULAR MEDICINE
(2021)
Review
Polymer Science
Iyyakkannu Sivanesan, Judy Gopal, Manikandan Muthu, Juhyun Shin, Selvaraj Mari, Jaewook Oh
Summary: This review discusses the preparation, drug delivery applications, and current status of green synthesis of chitosan. It highlights the lack of systematic research in the green synthesis of chitosan and calls for more innovative green processes to be explored. Additionally, there is a lack of reports on the application of green synthesized chitosan nanoforms and nanocomposites towards drug delivery applications, which is an area that deserves more research focus.
Review
Chemistry, Applied
Zohreh Mohammadi, Maryam Eini, Ali Rastegari, Morteza Rafiee Tehrani
Summary: Numerous studies have demonstrated the significant role of biomolecules in treating diseases, but the challenge lies in designing efficient delivery systems. Chitosan, a unique biopolymer with special properties, has been widely utilized for biomolecule delivery, with research groups focusing on its versatile application.
CARBOHYDRATE POLYMERS
(2021)
Article
Multidisciplinary Sciences
Jun Liu, Xiaoying Wang, Ann T. Chen, Xingchun Gao, Benjamin T. Himes, Hongyi Zhang, Zeming Chen, Jianhui Wang, Wendy C. Sheu, Gang Deng, Yang Xiao, Pan Zou, Shenqi Zhang, Fuyao Liu, Yong Zhu, Rong Fan, Toral R. Patel, W. Mark Saltzman, Jiangbing Zhou
Summary: Improved treatment of glioblastoma (GBM) can be achieved by inducing differentiation of glioblastoma stem cells (GSCs). The study identifies zinc finger protein 117 (ZNF117) as a regulator of GSC differentiation via Notch signaling through interaction with JAG2, and suggests that it can be targeted for therapy.
NATURE COMMUNICATIONS
(2022)
Review
Health Care Sciences & Services
Diogo Roque, Nuno Cruz, Hugo Alexandre Ferreira, Catarina Pinto Reis, Nuno Matela, Manuel Herculano-Carvalho, Rita Cascao, Claudia C. Faria
Summary: Glioblastoma is a malignant brain tumor with limited treatment options. Nanoparticles provide a promising approach for the treatment of glioblastoma by overcoming the blood-brain barrier and reducing systemic side effects.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Review
Chemistry, Multidisciplinary
Fakhria A. Al-Joufi, Aseem Setia, Mounir M. Salem-Bekhit, Ram Kumar Sahu, Fulwah Y. Alqahtani, Retno Widyowati, Fadilah Sfouq Aleanizy
Summary: This article addresses the molecular pathogenesis of colorectal cancer (CRC) and highlights the potential of biomarkers for predicting disease behavior and prognosis. It also provides conceptual knowledge of nanotechnology-based diagnostic techniques and therapeutic approaches for malignant CRC.
Article
Public, Environmental & Occupational Health
Hajar AlQahtani, Sara AlBilal, Ebrahim Mahmoud, Omar Aldibasi, Ahmad Alharbi, Nour Shamas, Abdulrahman Alsaedy, Kenana Owaidah, Fulwah Yahya Alqahtani, Fadilah Sfouq Aleanizy, Hatim Arishi, Saleem Baharoon, Mohammad Bosaeed
Summary: This study found that age 60 and above is the only factor associated with higher mortality rate in COVID-19 patients, regardless of the type of immunomodulator therapy. There is a lack of synergistic effect between TCZ and DEX on hospital mortality.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2022)
Article
Pharmacology & Pharmacy
Jouri S. Alanazi, Fulwah Yahya Alqahtani, Fadilah Sfouq Aleanizy, Awwad A. Radwan, Ahmed Bari, Qamraa Hamad Alqahtani, Hosam Gharib Abdelhady, Ibrahim Alsarra
Summary: This study aims to develop a new treatment modality for choroidal neovascularization (CNV) by utilizing targeted nanoparticles to deliver microRNA-539-5p (miR-539). The researchers found that miR-539 incorporated in nanoparticles reduced the viability of human retinal microvascular endothelial cells (HRMECs). Furthermore, functionalizing the nanoparticles with a specific targeting peptide enhanced the uptake of the nanoparticles.
Article
Nanoscience & Nanotechnology
Hosam Gharib Abdelhady, Fadilah Sfouq Aleanizy, Fulwah Yahya Alqahtani, Hamad Alkahtani
Summary: The effect of core length on the physicochemical properties and purity of PAMAM dendrimers was studied. Increasing the core length significantly changed the morphology of the dendrimers and improved their purity.
JOURNAL OF NANOMATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Ahad S. Abushal, Fadilah S. Aleanizy, Fulwah Y. Alqahtani, Faiyaz Shakeel, Muzaffar Iqbal, Nazrul Haq, Ibrahim A. Alsarra
Summary: Psoriatic arthritis is an autoimmune disease that causes joint inflammation and damage. The current treatments are limited in effectiveness and convenience. A study found that a self-nanoemulsifying drug delivery system (SNEDDS) improved the solubility and bioavailability of the medication Apremilast. The optimized formulation, F9, showed the highest drug release in vitro and further investigation is needed for its potential as an alternative delivery system.
Article
Pharmacology & Pharmacy
Esra Kamal Eltayb, Fadilah Sfouq Aleanizy, Fulwah Y. Alqahtani, Hamad M. Alkahtani, Siddique Akber Ansari, Ibrahim Alsarra
Summary: This study successfully prepared calcium alginate nanoparticles (CANPs) loaded with mBTL, a quorum and virulence inhibitor for Pseudomonas aeruginosa. The synthesized CANPs showed stable nano-size particles and sustained drug release profile. The mBTL-loaded-CANPs displayed stable structure and no interaction between mBTL and polymer. The findings suggest the potential of mBTL-loaded-CANPs in alleviating infections caused by Pseudomonas aeruginosa.
SAUDI PHARMACEUTICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Noha M. Soliman, Faiyaz Shakeel, Nazrul Haq, Fars K. Alanazi, Sultan Alshehri, Mohsen Bayomi, Ahmed S. M. Alenazi, Ibrahim A. Alsarra
Summary: In this study, statistical optimization of chitosan-based nanoparticles was conducted using Box-Behnken design and response surface methodology. The goal was to improve therapeutic delivery and enhance antimicrobial efficacy of ciprofloxacin hydrochloride. By optimizing formulation variables, the minimum inhibitory concentration of the nanoparticles was significantly decreased, while encapsulation efficiency and drug release were improved.
Article
Pharmacology & Pharmacy
Rehab A. Alshammari, Fadilah S. Aleanizy, Amal Aldarwesh, Fulwah Y. Alqahtani, Wael A. Mahdi, Bushra Alquadeib, Qamraa H. Alqahtani, Nazrul Haq, Faiyaz Shakeel, Hosam G. Abdelhady, Ibrahim A. Alsarra
Summary: This study developed RBX nanoparticles using PAMAM dendrimers for the treatment of diabetic retinopathy, providing a safe and effective method for RBX administration. The drug loading efficiency and in vitro release of the RBX:PAMAM dendrimer complexes were evaluated, and the 5:1 complex showed the highest loading efficiency and drug release. The nanoformulation improved the therapeutic outcomes of anti-VEGF therapy and the bioavailability of RBX for preventing vision loss in patients with diabetic retinopathy.
Article
Polymer Science
Esra Kamal Eltayb, Fulwah Yahya Alqahtani, Hamad M. Alkahtani, Ibrahim A. Alsarra, Rihaf Alfaraj, Fadilah Sfouq Aleanizy
Summary: Pseudomonas aeruginosa, a pathogen responsible for many chronic infections and resistant to multiple antibiotics, can be effectively targeted by meta-bromo-thiolactone (mBTL)-loaded calcium alginate nanoparticles (CANPs). These nanoparticles exhibit higher antibacterial and anti-virulence activity, reduce the biofilm formation and pyocyanin production of P. aeruginosa, and show low cytotoxic effects on human lung epithelial cells and cystic fibrosis fibroblast cells. It is suggested that mBTL-CANPs have great potential in prophylaxis and treatment of Pseudomonas aeruginosa infections.
Article
Chemistry, Physical
Faiyaz Shakeel, Nazrul Haq, Wael A. Mahdi, Ibrahim A. Alsarra, Sultan Alshehri, Miteb Alenazi, Abdulrahman Alwhaibi
Summary: This study investigated the solubility and thermodynamic analysis of isotretinoin in eleven different green solvents. The results showed that DMSO was the best solvent for solubilizing isotretinoin, especially at higher temperatures. The solubility of isotretinoin was found to increase with temperature.
Article
Biochemistry & Molecular Biology
Wafa K. Fatani, Fadilah S. Aleanizy, Fulwah Y. Alqahtani, Mohammed M. Alanazi, Abdullah A. Aldossari, Faiyaz Shakeel, Nazrul Haq, Hosam Abdelhady, Hamad M. Alkahtani, Ibrahim A. Alsarra
Summary: Lung cancer is the leading cause of cancer-related deaths worldwide. Erlotinib, a tyrosine kinase inhibitor, has been explored as a potential treatment for lung cancer. Researchers have formulated two different generations of polyamidoamine (PAMAM) dendrimers and evaluated their efficacy in delivering erlotinib. The results showed that the cationic G4 dendrimer had higher selectivity and antiproliferative activity against lung cancer cells compared to the neutral G5 dendrimer and erlotinib alone, suggesting its potential as a targeted and sustained release carrier for erlotinib.
Article
Chemistry, Analytical
Nazrul Haq, Muzaffar Iqbal, Afzal Hussain, Faiyaz Shakeel, Ashfaq Ahmad, Ibrahim A. Alsarra, Mohamed Fahad AlAjmi, Asra Mahfooz, M. Ali Abouzadeh
Summary: The objective of this study was to investigate the potential of biodegradable agro-industrial waste materials as biosorbents in solid-phase extraction (SPE) technique for sample preparation. Waste coffee husk (CH) powder was collected, treated chemically, and used as an SPE adsorbent to extract pantoprazole from wastewater samples. The efficiency and regeneration capability of CH were comparable to a commercially available silica-based C18 SPE adsorbent. The developed biosorbent showed a recovery range of 93.0% to 102.0% with a %RSD of 3.72% to 12.7%.
Article
Pharmacology & Pharmacy
Fulwah Yahya Alqahtani, Fadilah Sfouq Aleanizy, Hamad M. Alkahtani, Eram El Tahir, Siddique Akber Ansari, Atheer Alharbi, Asmaa Al-Bdrawy, Faiyaz Shakeel, Nazrul Haq, Lamees S. Al-Rasheed, Rihaf Alfaraj, Abdullah K. Alshememry, Ibrahim A. Alsarra
Summary: This study successfully prepared chitosan nanoparticles (CNPs) loaded with C3-005, which showed high activity against pneumococcal infection and reduced bacterial hemolysis.
SAUDI PHARMACEUTICAL JOURNAL
(2023)
