Article
Cell Biology
Beatriz Almaran, Guillem Ramis, Silvia Fernandez de Mattos, Priam Villalonga
Summary: In this study, the functional crosstalk between receptor tyrosine kinases (RTKs) and Rho GTPases was analyzed, with a focus on the specific contribution of Rnd3 to the invasive phenotype of glioblastoma cells. RTK inhibition decreased cell motility and invasion, and activated the RhoA/ROCK pathway for actin cytoskeletal reorganization. Rnd3 depletion had similar effects to RTK inhibition, suggesting its crucial role in regulating the invasive phenotype of glioblastoma cells through actin cytoskeletal reorganization.
Review
Oncology
Pontus Aspenstrom
Summary: For many years, the role of RHO GTPases in cancer has been overlooked, but recent studies have shown that they are altered in many cancer types and may have oncogenic effects. This review article summarizes the current knowledge of these mutant RHO GTPases, focusing on how their kinetics can contribute to cancer progression.
Article
Chemistry, Physical
Mainak Chatterjee, Anirban Sain
Summary: Dynamic pattern formations in multicellular systems and embryos at their single-cell stage can be modeled using reaction-diffusion networks.
JOURNAL OF CHEMICAL PHYSICS
(2022)
Article
Chemistry, Medicinal
Jing Xu, Federica Simonelli, Xiaoyun Li, Angelo Spinello, Sara Laporte, Vincent Torre, Alessandra Magistrato
Summary: The study successfully reduced the infiltration propensity of GBM by using inhibitors of two small Rho GTPases, with all-atom simulations elucidating the molecular basis of their inhibition mechanism and providing valuable information for future drug discovery studies.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2021)
Article
Oncology
Dana El-Chami, Maria Al Haddad, Ralph Abi-Habib, Mirvat El-Sibai
Summary: Breast cancer is a major cause of death in women worldwide, with targeting breast cancer cell metastasis being an important therapeutic approach. The MAPK pathway has been identified as a key player in cellular invasion and migration. In this study, treatment with anthrax lethal toxin (LeTx) inhibited breast cancer cell migration by affecting the activation of RhoA and Cdc42, leading to decreased invasion and increased adhesion.
Article
Multidisciplinary Sciences
Lucie Plazen, Jalal Al Rahbani, Claire M. M. Brown, Anmar Khadra
Summary: In mesenchymal cell motility, Rac, Rho, and paxillin play important roles in forming migration patterns and regulating adhesion dynamics. The interactions among these proteins generate wave-pinning and cell polarization, but their collective effect on other migration modes in CHO-K1 cells is still unclear. By using a computational model and image analysis, this study explores how differences in timescales within the molecular network can produce migration patterns and switching between modes.
SCIENTIFIC REPORTS
(2023)
Article
Pharmacology & Pharmacy
Judyta Gorka, Paulina Marona, Oliwia Kwapisz, Janusz Rys, Jolanta Jura, Katarzyna Miekus
Summary: This study investigated the role of MCPIP1 in ccRCC cell migration and found that MCPIP1 inhibits ccRCC cell migration by reducing the levels of Rho GTPases and the phosphorylation of FAK and Src. The loss of MCPIP1 RNase activity leads to increased expression of IL-1 beta and changes in the expression of genes correlated with migration as tumor progression occurs in ccRCC patients.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Developmental Biology
Mirvat El-Sibai, Joelle El Hajj, Maria Al Haddad, Nada El Baba, Mounir Al Saneh, Wassim Daoud Khatoun, Raphael Helaers, Miikka Vikkula, Oula El Atat, Joseph Sabbagh, Naji Abou Chebel, Michella Ghassibe-Sabbagh
Summary: Cleft lip and palate result from the improper migration and assembly of lip buds and palatal shelves during embryogenesis. The dysregulation of Rho GTPases may be correlated with the observed phenotype of cleft lip and palate patient cells. Additionally, patient cells show decreased migration, increased adhesion, and a more elongated phenotype.
CELLS & DEVELOPMENT
(2021)
Article
Cell Biology
Gabriel Kreider-Letterman, Nicole M. Carr, Rafael Garcia-Mata
Summary: Cancer progression and metastasis involve cellular changes, including alterations in Rho GTPase family protein activity. Abnormal Rho GTPase signaling is associated with cancer development. Rho GTPase signaling is regulated by guanine nucleotide-exchange factors (RhoGEFs) and GTPase activating proteins (RhoGAPs). RhoGAP family members are emerging as key regulators of cancer development and progression.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell Biology
Kathryn N. Becker, Krista M. Pettee, Amanda Sugrue, Kevin A. Reinard, Jason L. Schroeder, Kathryn M. Eisenmann
Summary: Targeting mDia is an effective strategy against invasion and tumor microtube networking, while inhibiting ROCK is contraindicated for treating brain cancer.
Article
Cell Biology
Leila Jaafar, Isabelle Fakhoury, Sahar Saab, Layal El-Hajjar, Wassim Abou-Kheir, Mirvat El-Sibai
Summary: Prostate cancer is the second most diagnosed cancer in men globally. The Rho family of GTPases, including StarD13, play a significant role in regulating cancer cell proliferation and migration. StarD13 acts as a tumor suppressor inhibiting prostate cancer cell invasion by regulating Cdc42.
Article
Biochemistry & Molecular Biology
Yuki Miyamoto, Tomohiro Torii, Keiichi Homma, Hiroaki Oizumi, Katsuya Ohbuchi, Kazushige Mizoguchi, Shou Takashima, Junji Yamauchi
Summary: This study found that the phosphorylation status of Tyr(381) in cytohesin-2 plays a central role in Schwann cell myelination in the peripheral nervous system. The signaling unit centered on cytohesin-2, with SH2B1 as a positive regulator and PTP4A1 as a negative regulator, controls Schwann cell myelination.
Article
Multidisciplinary Sciences
Stanislav Kholmanskikh, Shawn Singh, M. Elizabeth Ross
Summary: Regulation of Rho GTPases is actively studied due to their essential roles in cell biology and the pathophysiology of human diseases. This study reveals that RhoC, but not RhoA, is targeted by non-degrading ubiquitination (NDU) mediated by E3 ubiquitin ligase LNX1. In addition, LIS1 negatively regulates LNX1 ubiquitination of RhoC. The inhibition of LNX1's effects on RhoGDI-RhoC interaction by LIS1 provides a molecular mechanism for the enhanced activity of Rho proteins upon reduction in LIS1 protein levels.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Geunhye Hong, Cadi Davies, Zahra Omole, Janie Liaw, Anna D. D. Grabowska, Barbara Canonico, Nicolae Corcionivoschi, Brendan W. W. Wren, Nick Dorrell, Abdi Elmi, Ozan Gundogdu
Summary: Campylobacter jejuni is the main bacterial cause of worldwide foodborne gastroenteritis and its interaction with the defense mechanisms of human intestinal epithelial cells (IECs) is not well understood. This study shows that C. jejuni regulates intracellular and extracellular ROS production in IECs and downregulates the expression of key ROS-generating enzyme NADPH oxidase, as well as antioxidant defense genes CAT and SOD1. Inhibition of NADPH oxidase reduces C. jejuni's ability to interact and survive within IECs. These findings provide mechanistic insight into how C. jejuni modulates the defense machinery of IECs.
CELLULAR MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Massimo Pancione, Luigi Cerulo, Andrea Remo, Guido Giordano, Alvaro Gutierrez-Uzquiza, Paloma Bragado, Almudena Porras
Summary: Metastasis is a crucial process in cancer development, closely linked to chromosomal instability and centrosome malfunction. Abnormalities in the centrosome can lead to cellular development issues and increase cancer risk.
Review
Oncology
Sabreen F. Fostok, Mirvat El-Sibai, Marwan El-Sabban, Rabih S. Talhouk
JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA
(2019)
Article
Oncology
Ghenwa Nasreddine, Mirvat El-Sibai, Ralph J. Abi-Habib
INVESTIGATIONAL NEW DRUGS
(2020)
Article
Immunology
Souraya Sayegh, Oula El Atat, Katy Diallo, Benjamin Rauwel, Yannick Degboe, Etienne Cavaignac, Arnaud Constantin, Alain Cantagrel, Viviane Trak-Smayra, Nada Alaaeddine, Jean-Luc Davignon
FRONTIERS IN IMMUNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Hamid E. Daaboul, Carole Dagher, Robin Taleb, Kikki Bodman-Smith, Wassim N. Shebaby, Mirvat El-Sibai, Mohamad A. Mroueh, Costantine F. Daher
CHEMICO-BIOLOGICAL INTERACTIONS
(2019)
Correction
Immunology
Souraya Sayegh, Oula El Atat, Katy Diallo, Benjamin Rauwel, Yannick Degboe, Etienne Cavaignac, Arnaud Constantin, Alain Cantagrel, Viviane Trak-Smayra, Nada Alaaeddine, Jean-Luc Davignon
FRONTIERS IN IMMUNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Houssam Al-Koussa, Maria Al-Haddad, Ralph Abi-Habib, Mirvat El-Sibai
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Cell Biology
Samer Nicolas, Sandra Abdellatef, Maria Al Haddad, Isabelle Fakhoury, Mirvat El-Sibai
Review
Oncology
Houssam Al-Koussa, Nour El Mais, Hiba Maalouf, Ralph Abi-Habib, Mirvat El-Sibai
CANCER CELL INTERNATIONAL
(2020)
Article
Cell Biology
Mirna Swayden, Amira Bekdash, Isabelle Fakhoury, Oula El-Atat, Jamila Borjac-Natour, Mirvat El-Sibai, Ralph J. Abi-Habib
Summary: The study demonstrates that colon cancer cells are partially auxotrophic for arginine and sensitive to arginine deprivation induced by [HuArgI (Co)-PEG5000], which leads to cell death. Additionally, arginine deprivation results in activation of autophagy, contributing to cytotoxicity and cell death.
Review
Cardiac & Cardiovascular Systems
Ibrahim AlZaim, Safaa H. Hammoud, Houssam Al-Koussa, Alaa Ghazi, Ali H. Eid, Ahmed F. El-Yazbi
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2020)
Review
Medicine, General & Internal
Houssam Al-Koussa, Ibrahim AlZaim, Marwan E. El-Sabban
Summary: Blood coagulation is a complex physiological process involving the activation of coagulation factors and intercellular communication. Imbalance in coagulation can lead to bleeding or thrombosis, and extracellular vesicles (EVs) may represent valuable approaches for the treatment and diagnosis of thrombotic diseases.
JOURNAL OF CLINICAL MEDICINE
(2022)
