4.8 Article

Evaluating the stability of three oxidative stress biomarkers under sewer conditions and potential impact for use in wastewater-based epidemiology

Journal

WATER RESEARCH
Volume 166, Issue -, Pages -

Publisher

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2019.115068

Keywords

PGF(2 alpha); dnPGF(2 alpha); PGE(2); Oxidative stress; In-sewer degradation; Biomarkers

Funding

  1. Queensland Alliance for Environmental Health Sciences, The University of Queensland of the Queensland Health
  2. University of Queensland Early Career Researcher Grant [UQECR1834613]
  3. Australian Research Council [DP150100645, DP180101475]
  4. Australian Government Research Training Program (RTP) Scholarship
  5. China Scholarship Council
  6. Australian Research Council DECRA Fellowship [DE170100694]

Ask authors/readers for more resources

Wastewater contains a wealth of information about the population who contribute to it including biological and chemical markers of human activity and exposures. F-2-isoprostanes have been proposed as oxidative stress biomarkers that can be measured in wastewater to provide a measure of oxidative stress at the population level. While an association between tobacco use and their level in wastewater has been demonstrated, an in-sewer stability assessment has not been conducted to support their use as oxidative stress biomarkers for wastewater-based epidemiology studies. In this study we investigated the stability of 8-iso-prostaglandin F-2 alpha (PGF(2 alpha)), its metabolite dinor-11 beta-Prostaglandin F-2 alpha (dnPGF(2 alpha)) and Prostaglandin E-2 (PGE(2)) (representative of other classes of prostaglandins) in laboratory-scale sewer reactors simulating real sewers. PGF(2 alpha), dnPGF(2 alpha) and PGE(2) were all found to be sufficiently stable under typical sewer conditions therefore satisfying the stability requirement of wastewater-based epidemiology population health biomarkers. (C) 2019 Elsevier Ltd. All rights reserved.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.8
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Review Environmental Sciences

Enhancing integrated denitrifying anaerobic methane oxidation and Anammox processes for nitrogen and methane removal: A review

Yan Chen, Guangming Jiang, Muttucumaru Sivakumar, Jiangping Wu

Summary: This review summarizes the integration of denitrifying anaerobic methane oxidation (DAMO) and anaerobic ammonium oxidation (Anammox) for wastewater treatment, evaluates the challenges and strategies for their full-scale application, and proposes a conceptual design of innovative wastewater treatment plants based on the integrated DAMO-Anammox.

CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY (2023)

Article Substance Abuse

A wastewater-based evaluation of the effectiveness of codeine control measures in Australia

Benjamin J. Tscharke, Jake W. O'Brien, Fahad Ahmed, Lynn Nguyen, Maulik Ghetia, Gary Chan, Phong Thai, Cobus Gerber, Richard Bade, Jochen Mueller, Kevin V. Thomas, Jason White, Wayne Hall

Summary: Wastewater-based epidemiology was used to measure changes in population codeine consumption in Australia after it was rescheduled from an over-the-counter to a prescription-only medicine in 2018. The study found that the average per-capita consumption of codeine decreased by approximately 37% nationally, with significant decreases observed in all states. Areas with less pharmacy access experienced larger decreases in consumption compared to areas with greater pharmacy access. Wastewater analysis can effectively evaluate changes in population pharmaceutical consumption in response to drug scheduling changes.

ADDICTION (2023)

Article Engineering, Environmental

National Wastewater Reconnaissance of Analgesic Consumption in Australia

Fahad Ahmed, Benjamin Tscharke, Jake W. O'Brien, Wayne D. Hall, Peter J. Cabot, P. Marcin Sowa, Saer Samanipour, Kevin V. Thomas

Summary: A wastewater-based epidemiology method was used to estimate analgesic consumption and assess pain burden in Australian communities. The concentration of analgesics in wastewater samples from 60 communities was analyzed, and the amount of drug consumed per day per 1000 inhabitants was estimated. The burden of pain treatment was categorized as mild to moderate or strong to severe pain, and a higher burden was observed in regional and remote areas. The correlation analysis showed that pain affects populations across socioeconomic groups. Australians spent approximately US $3.5 per day on analgesics. The study suggests that wastewater-based epidemiology can be an effective tool to estimate analgesic consumption and assess pain burden in communities.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2023)

Article Engineering, Environmental

Wastewater-based epidemiology for the assessment of population exposure to chemicals: The need for integration with human biomonitoring for global One Health actions

Barbara Kasprzyk-Hordern, Frederic Been, Lubertus Bijlsma, Werner Brack, Sara Castiglioni, Adrian Covaci, Bice S. Martincigh, Jochen F. Mueller, Alexander L. N. van Nuijs, Temilola Oluseyi, Kevin V. Thomas

Summary: Water-based environmental monitoring (WBE) has become a useful tool for SARS-CoV-2 surveillance and has been applied to assess illicit drug consumption in communities. It is important to expand its use to evaluate community exposure to chemical stressors. The goal of WBE is to measure exposure, identify exposure-outcome associations, and implement interventions for exposure prevention and public health promotion. To fully utilize WBE, further action is needed, such as integrating WBE with human biomonitoring initiatives, conducting global WBE monitoring campaigns, combining WBE with One Health actions, and advancing analytical tools and methodologies.

JOURNAL OF HAZARDOUS MATERIALS (2023)

Article Environmental Sciences

Use of artificial sweeteners and caffeine in a population of Hanoi: An assessment by wastewater-based epidemiology

Dandan Li, Qiuda Zheng, Kevin V. Thomas, Anh Kim Dang, Vu Ngan Binh, Nguyen Thi Kieu Anh, Phong K. Thai

Summary: Wastewater analysis was used to evaluate the consumption of artificial sweeteners in an urban population in Hanoi, Vietnam. The study found that the daily consumption of artificial sweeteners was stable throughout the week, but varied with different seasons. There were significant correlations between artificial sweeteners and caffeine, indicating common sources.

SCIENCE OF THE TOTAL ENVIRONMENT (2023)

Article Environmental Sciences

Moving forward with COVID-19: Future research prospects of wastewater-based epidemiology methodologies and applications

Guangming Jiang, Yanchen Liu, Song Tang, Masaaki Kitajima, Eiji Haramoto, Sudipti Arora, Phil M. Choi, Greg Jackson, Patrick M. D'Aoust, Robert Delatolla, Shuxin Zhang, Ying Guo, Jiangping Wu, Yan Chen, Elipsha Sharma, Tanjila Alam Prosun, Jiawei Zhao, Manish Kumar, Ryo Honda, Warish Ahmed, Jon Meiman

Summary: Wastewater-based epidemiology (WBE) has the potential to track COVID-19 transmission, but faces methodological limitations. Future research should focus on standardizing sampling and analysis protocols, understanding viral stability in sewer systems, and utilizing artificial intelligence and big data models for smart wastewater surveillance. Further research is also needed for monitoring other viral pathogens and applying WBE beyond COVID-19.

CURRENT OPINION IN ENVIRONMENTAL SCIENCE & HEALTH (2023)

Article Environmental Sciences

In-Sample Stability of 52 Performance- and Image-Enhancing Drug Biomarkers in Wastewater

Katja M. Shimko, Jake W. O'Brien, Xialu Lin, Benjamin J. Tscharke, Kevin Thomas

Summary: Wastewater analysis shows potential in tracking the use of performance- and image-enhancing drugs in communities, but the stability of samples after sampling is currently unknown. This study conducted stability experiments on 52 biomarkers of such drugs in wastewater, testing different conditions including pretreatments, sample preservation methods, and temperatures. The results indicate that filtration and acidification to pH 2 are the optimal preservation methods, with stability ranging from medium to high for up to 200 days. Additionally, a calculator was provided to assist in sample collection and archiving.

ACS ES&T WATER (2023)

Article Chemistry, Analytical

Cumulative Neutral Loss Model for Fragment Deconvolution in Electrospray Ionization High-Resolution Mass Spectrometry Data

Denice van Herwerden, Jake W. O'Brien, Sascha Lege, Bob W. J. Pirok, Kevin V. Thomas, Saer Samanipour

Summary: Clean high-resolution mass spectra are crucial for the successful structural elucidation of unknown features in non-target analysis workflows. An algorithm combining time domain and mass domain information is presented for spectral deconvolution, achieving high true positive rates and reduction rates in real samples. The combination of correlation analysis and the cumulative neutral loss model proved to be the most effective for fragment deconvolution.

ANALYTICAL CHEMISTRY (2023)

Article Engineering, Environmental

Novel Per- and Polyfluoroalkyl Substances Discovered in Cattle Exposed to AFFF-Impacted Groundwater

Pradeep Dewapriya, Sandra Nilsson, Sara Ghorbani Gorji, Jake W. O'Brien, Jennifer Braunig, Maria Jose Gomez Ramos, Eric Donaldson, Saer Samanipour, Jonathan W. Martin, Jochen F. Mueller, Sarit L. Kaserzon, Kevin V. Thomas

Summary: Leaching of PFASs from Australian firefighting training grounds has resulted in extensive contamination. A study identified various PFAS classes in cattle's blood exposed to contaminated groundwater and discovered novel PFASs. Results suggest that targeting only major PFASs underestimates the toxicological risks associated with exposure.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2023)

Article Engineering, Environmental

InSpectra-A platform for identifying emerging chemical threats

Mathieu Feraud, Jake W. O'Brien, Saer Samanipour, Pradeep Dewapriya, Denice van Herwerden, Sarit Kaserzon, Ian Wood, Cassandra Rauert, Kevin V. Thomas

Summary: Non-target analysis (NTA) using high-resolution mass spectrometry (HRMS) coupled with liquid chromatography is increasingly employed for identifying biologically relevant chemicals. Interpreting HRMS datasets, which are often recorded in vendor-specific formats, can be challenging due to their large and complex nature. InSpectra is a web-based, open-source platform that offers flexible and extensible NTA workflows for the systematic detection of emerging chemical threats. It provides a reproducible and transparent approach for identifying, tracking, and prioritizing these threats.

JOURNAL OF HAZARDOUS MATERIALS (2023)

Article Construction & Building Technology

Long-Term Self-Healing Efficiency of Bioconcrete Based on Integrated Sulfate- and Nitrate-Reducing Bacterial Granules

Kirthi Chetty, Ulf Garbe, Timothy McCarthy, Faisal Hai, Guangming Jiang

Summary: This study evaluated the mechanical properties and self-healing performance of freshly casted and 19-month-aged bioconcrete samples with integrated sulfate-reducing bacteria (SRB) and nitrate-reducing bacteria (NRB) granules that were cultivated in an upflow anaerobic sludge blanket (UASB) reactor with synthetic wastewater. The results showed that both fresh and aged bioconcrete met the design requirement of 28-day compressive strength and had a low volume of permeable voids. The self-healing ability of the bioconcrete was demonstrated by the deposition of calcite on cracked samples after exposure to different water media.

JOURNAL OF MATERIALS IN CIVIL ENGINEERING (2023)

Article Engineering, Environmental

Evaluation of sealants to mitigate the release of per- and polyfluoroalkyl substances (PFAS) from AFFF-impacted concrete: Characterization and forecasting

Phong H. N. Vo, Trent A. Key, Tu Hoang Le, Jeffrey T. Mcdonough, Scott Porman, Stephanie Fiorenza, Hong T. M. Nguyen, Vinh T. N. Dao, Jochen F. Mueller, Phong K. Thai

Summary: This study aimed to assess the effectiveness of two commercially available sealants in reducing the leaching of PFAS from concrete surfaces and develop a model to predict the cumulative leaching of five PFAS over 20 years. The results showed that both sealants reduced the leaching of PFAS, with Product B performing better than Product A. The model predicted a cumulative leaching of about 400 mg/m(2) of PFOS from an unsealed concrete surface over 20 years, while sealing with Product A resulted in an estimated leakage of 85% of PFOS (approximately 340 mg/m(2)), and negligible leaching was observed after sealing with Product B (< 5 x 10(-9) mg/m(2)).

WATER RESEARCH X (2023)

Review Environmental Sciences

Wastewater-based epidemiology of Campylobacter spp.: A systematic review and meta-analysis of influent, effluent, and removal of wastewater treatment plants

Shuxin Zhang, Jiahua Shi, Xuan Li, Ananda Tiwari, Shuhong Gao, Xu Zhou, Xiaoyan Sun, Jake W. O'Brien, Lachlan Coin, Faisal Hai, Guangming Jiang

Summary: This study conducted a meta-analysis and systematic review on the prevalence of Campylobacter spp. in different wastewater samples. The results showed that the overall prevalence of Campylobacter spp. was 52.97% in all types of wastewater samples, with the highest concentration in the influent wastewater during autumn. Campylobacter jejuni, Campylobacter coli, and Campylobacter lari were the most prevalent species found in wastewater.

SCIENCE OF THE TOTAL ENVIRONMENT (2023)

Article Substance Abuse

Increased Nicotine Consumption in Australia During the First Months of the COVID-19 Pandemic

Phong K. Thai, Benjamin J. Tscharke, Jake O'Brien, Coral Gartner, Richard Bade, Cobus Gerber, Jason M. White, Qiuda Zheng, Zhe Wang, Kevin Thomas, Jochen F. Mueller

Summary: The study aimed to quantify changes in smoking prevalence in Australia through nicotine consumption. The findings showed that although smoking prevalence decreased from 2017 to 2019, it increased in 2020, particularly during the early stage of the pandemic. This may be due to higher stress levels and increased opportunities to smoke/vape while working from home and during lockdowns.

NICOTINE & TOBACCO RESEARCH (2023)

Article Engineering, Environmental

Fate of micropollutants in a lab-scale urban wastewater system: Impact of iron-rich drinking water treatment sludge

Jagadeeshkumar Kulandaivelu, Yan Chen, Phil M. Choi, Xuan Li, Mario Rebosura Jr, Yarong Song, Zhiguo Yuan, Jochen F. Mueller, Guangming Jiang

Summary: Iron rich drinking water treatment sludge (Fe-DWTS) can be a potential alternative to control sulfide in the sewer network. Fe-DWTS dosed sewer reactor showed higher removal of some organic micropollutants, while the removal of others decreased along with the decrease in biofilm methanogenic activity. Long-term experiments showed no significant effect of in-sewer dosed Fe-DWTS on the removal of most micropollutants, except for a few compounds which showed slightly higher concentrations in the effluent possibly due to change in microbial composition.

JOURNAL OF HAZARDOUS MATERIALS ADVANCES (2023)

Article Engineering, Environmental

Parabens and their metabolite in a marine benthic-dominated food web from the Beibu gulf, South China Sea: Occurrence, trophic transfer and health risk assessment

Rong-Gui Zhu, Chang-Gui Pan, Feng-Jiao Peng, Chao-Yang Zhou, Jun-Jie Hu, Kefu Yu

Summary: This comprehensive survey investigated the occurrence, bioaccumulation, and trophic magnification of parabens and their metabolite 4-HB in a marine food web. Results showed that parabens were the predominant pollutants in marine organisms, with significant bioaccumulation from sediments. The estimated trophic magnification factor indicated biomagnification for MeP and trophic dilution for 4-HB. Overall, the risks for humans consuming marine organisms were found to be low.

WATER RESEARCH (2024)

Article Engineering, Environmental

Partitioning and inactivation of enveloped and nonenveloped viruses in activated sludge, anaerobic and microalgae-based wastewater treatment systems

Andres F. Torres-Franco, Deborah Leroy-Freitas, Cristina Martinez-Fraile, Elisa Rodriguez, Pedro A. Garcia-Encina, Raul Munoz

Summary: Anaerobic and microalgae-based technologies have emerged as sustainable alternatives for municipal wastewater treatment. However, the presence of viruses in the treated wastewater is a major concern for reuse applications. This study assessed the ability of these technologies to reduce viruses during secondary wastewater treatment. The results showed that all technologies were effective in reducing the concentration of viruses, with microalgae-based treatment exhibiting the highest potential for reducing the disinfection requirements of treated wastewater.

WATER RESEARCH (2024)

Article Engineering, Environmental

Reconsidering mercury sources and exposure pathways to bivalves: Insights from mercury stable isotopes

Young Gwang Kim, Sae Yun Kwon, Spencer J. Washburn, Scott C. Brooks, Ji Won Yoon, Lucien Besnard

Summary: The study uses Hg isotope ratios to identify the sources and exposure pathways of mercury in bivalves, finding that dissolved Hg phases in the water column are the primary source and exposure pathway to bivalves. This provides new insights into using bivalves as bioindicators for sediment quality monitoring.

WATER RESEARCH (2024)

Article Engineering, Environmental

Cation exchange resins enhance anaerobic digestion of sewage sludge: Roles in sequential recovery of hydrogen and methane

Hui Geng, Ying Xu, Rui Liu, Dianhai Yang, Xiaohu Dai

Summary: This study investigates the effect of cation exchange resin (CER) on the sequential recovery of hydrogen and methane from anaerobic digestion (AD) and the corresponding mechanisms. The results show that CER can simultaneously enhance the production of hydrogen and methane by promoting the solubilisation, hydrolysis, and acidification of organic matter. Additionally, CER facilitates effective contact between bacteria and organic particulates and reduces the energy barrier for mass transfer during methane production. The study also reveals changes in the microbial community structure and metagenomics during the AD process.

WATER RESEARCH (2024)

Article Engineering, Environmental

Fertilizer recovery from source-separated urine by evaporation with a combined process of dehumidification and the addition of absorbent resin supplement

Xiaojing Lin, Zhan Jin, Shunfeng Jiang, Zhiquan Wang, Suqing Wu, Ke Bei, Min Zhao, Xiangyong Zheng

Summary: Dehumidification combined with addition of absorbent resin supplement (ARS) was used to achieve rapid evaporation of non-pretreated urine, resulting in high water evaporation efficiency and nutrient recovery.

WATER RESEARCH (2024)

Article Engineering, Environmental

Influences of hydrodynamics on microbial community assembly and organic carbon composition of resuspended sediments in shallow marginal seas

Yangli Che, Chaoran Lin, Shen Li, Jiao Liu, Longhai Zhu, Shilei Yu, Nan Wang, Haoshuai Li, Mutai Bao, Yang Zhou, Tonghao Si, Rui Bao

Summary: Hydrodynamic processes play a crucial role in the transmission of sediments, microbial assembly, and organic carbon redistribution in the ocean. Through experiments and analysis, we found that hydrodynamics shape the assembly of microbial communities and control the redistribution of different sourced organic carbon, thereby influencing microbial-mediated biogeochemical transformation.

WATER RESEARCH (2024)

Article Engineering, Environmental

A comprehensive evaluation of the temporal and spatial fouling characteristics of RO membranes in a full-scale seawater desalination plant

Chao Chen, Yu Yang, Nigel J. D. Graham, Zhenyu Li, Xingtao Yang, Zhining Wang, Nadia Farhat, Johannes S. Vrouwenvelder, Li -an Hou

Summary: The fouling of seawater reverse osmosis membranes is a persistent challenge in desalination. This study monitored the operational performance of a desalination plant for 7 years and the fouling development in different areas of membrane modules. The findings showed that operational performance declined over time and fouling mainly occurred at the feed side of the modules, with the highest microbial diversity. Keystone species like Chloroflexi and Planctomycetes played an important role in maintaining community structure and biofilm maturation. Polysaccharides, soluble microbial products, marine humic acid-like substances, and inorganic substances contributed to fouling. Overall, biofouling had a significant impact on membrane fouling after 7 years of operation.

WATER RESEARCH (2024)

Article Engineering, Environmental

Fluctuating redox conditions accelerate the electron storage and transfer in magnetite and production of dark hydroxyl radicals

Dan Li, Jieyi Sun, Yibo Fu, Wentao Hong, Heli Wang, Qian Yang, Junhong Wu, Sen Yang, Jianhui Xu, Yunfei Zhang, Yirong Deng, Yin Zhong, Ping'an Peng

Summary: Sulfidation-oxidation treatment of magnetite (Fe3O4) enhances the production of dark center dot OH, which can efficiently degrade dissolved organic matter (DOM) and accelerate carbon cycling.

WATER RESEARCH (2024)

Article Engineering, Environmental

Full-scale upgrade activated sludge to continuous-flow aerobic granular sludge: Implementing microaerobic-aerobic configuration with internal separators

Cheng Yu, Kaijun Wang, Kaiyuan Zhang, Ruiyang Liu, Pingping Zheng

Summary: This study implemented a microaerobic-aerobic configuration in a full-scale municipal wastewater treatment facility and investigated the effects on sludge characteristics, pollutant removal, microbial community, and granulation mechanisms. The results showed successful transition from flocculent-activated sludge to well-defined AGS after two months of operation. The primary pathways for pollutant removal were simultaneous nitrification, denitrification, and phosphorus removal. Moreover, the incorporation of internal separators induced shifts in the flow pattern, which promoted granulation.

WATER RESEARCH (2024)

Article Engineering, Environmental

Target analysis, occurrence and cytotoxicity of halogenated polyhydroxyphenols as emerging disinfection byproducts in drinking water

Zhe Zhang, Shaoyang Hu, Guangrong Sun, Wei Wang

Summary: Halogenated aromatic disinfection byproducts (DBPs), such as halogenated phenols, have garnered widespread attention due to their high toxicity and prevalence. This study reports on the analysis, occurrence, and cytotoxicity of a group of emerging halogenated aromatic DBPs, known as halogenated polyhydroxyphenols (HPPs), in drinking water.

WATER RESEARCH (2024)

Article Engineering, Environmental

A coupled model to improve river water quality prediction towards addressing non-stationarity and data limitation

Shengyue Chen, Jinliang Huang, Peng Wang, Xi Tang, Zhenyu Zhang

Summary: Accurate prediction of river water quality is crucial for sustainable water management. This study introduces wavelet analysis and transfer learning techniques to assist LSTM modeling, proposing a newly coupled modeling approach that improves short-term prediction of river water quality.

WATER RESEARCH (2024)

Article Engineering, Environmental

Deciphering anaerobic ethanol metabolic pathways shaped by operational modes

Bang Du, Xinmin Zhan, Piet N. L. Lens, Yifeng Zhang, Guangxue Wu

Summary: Efficient anaerobic digestion relies on the cooperation of different microorganisms with different metabolic pathways. This study investigated the effects of different operational modes and the addition of powdered activated carbon (PAC) on ethanol metabolic pathways. The results showed that the SBR mode and the presence of CO2 facilitated ethanol metabolism towards propionate production, while the CFR mode with extended solids retention time enriched Geobacter. Adjusting operational modes and PAC addition can modulate anaerobic ethanol metabolism and enrich Geobacter.

WATER RESEARCH (2024)

Article Engineering, Environmental

Unraveling the factors influencing CO2 emissions from hydroelectric reservoirs in karst and non-karst regions: A comparative analysis

Wanfa Wang, Si-Liang Li, Jun Zhong, Yuanbi Yi, Fujun Yue, Zenglei Han, Qixin Wu, Ding He, Cong-Qiang Liu

Summary: This study compares the carbon biogeochemical processes in karst and non-karst regions within large thermal stratified river-reservoir systems. The results demonstrate that karst reservoirs have a reduced potential for carbon emissions and highlight the importance of considering geologic settings to improve accuracy in regional and global CO2 emission estimates.

WATER RESEARCH (2024)

Article Engineering, Environmental

Rare resistome rather than core resistome exhibited higher diversity and risk along the Yangtze River

Chunxia Jiang, Zelong Zhao, Dong Zhu, Xiong Pan, Yuyi Yang

Summary: This study analyzed the occurrence and distribution of antibiotic resistance genes (ARGs) in different environmental media of the Yangtze River using metagenomics. Core resistome dominated by multidrug resistance genes was found in all samples, while rare resistome dominated by various resistance genes was more prevalent in plasmids. Specific bacteria were identified as hosts for both core and rare resistomes, with high clinical concern ARGs found in the rare resistome. Particle-associated environment provided the most ideal conditions for resistome hosts. This study provided insights into the genetic locations of ARGs and the community assembly mechanisms of ARG hosts in freshwater environments.

WATER RESEARCH (2024)

Article Engineering, Environmental

Uncovering interactions among ternary electron donors of organic carbon source, thiosulfate and Fe0 in mixotrophic advanced denitrification: Proof of concept from simulated to authentic secondary effluent

Yu Zhang, Yongtao He, Linchun Jia, Lei Xu, Zheng Wang, Yueling He, Ling Xiong, Xumeng Lin, Hong Chen, Gang Xue

Summary: By synergizing organic carbon source, thiosulfate, and zero-valent iron, efficient mixotrophic denitrification of oligotrophic secondary effluent can be achieved. Thiosulfate plays a vital role in promoting TN removal efficiency, while corrosion of Fe0 releases OH- to neutralize H+ from thiosulfate-driven denitrification, creating a suitable environment for denitrification. The coordination of thiosulfate and Fe0 maintains the dominance of Thiobacillus for denitrification.

WATER RESEARCH (2024)