Article
Chemistry, Analytical
Yadi Wang, Wentao Dai, Zhixiao Liu, Jixiang Liu, Jie Cheng, Yuanyuan Li, Xueling Li, Jun Hu, Junhong Lu
Summary: A method based on single-cell FTIR microspectroscopy was developed to quantitatively evaluate cellular heterogeneity by calculating the cell-to-cell similarity distance, revealing that infrared phenotypes may reflect dynamic heterogeneity changes in cell populations. This provides an alternative label-free optical approach for quantifying cellular heterogeneity and can be combined with other single-cell analysis tools to understand the genotype-to-phenotype relationship in cellular populations.
ANALYTICAL CHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Zhi Jin, Weili Cui, Jianfeng Ma, Qian Chen, Yuejin Fu
Summary: Confocal Raman microspectroscopy was used to observe the dissolution of hydroxycinnamates (HCMs) incorporated into lignin-carbohydrate complexes (LCCs) in energy crops Miscanthus sinensis cv. during NaOH treatment. The results showed that mild NaOH treatment led to a higher proportion of HCM depolymerization in highly lignified middle lamella areas compared to carbohydrate-abundant secondary walls. Raman imaging revealed that lignin depolymerization was more pronounced in the sclerenchyma fiber and parenchyma secondary wall with increasing treatment time, while the depolymerization of HCMs was closely related to lignin depolymerization. A better understanding of these processes is important for efficient breaking of LCC bonds in herbaceous biomass.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Shumpei Horii, Ashok Zachariah Samuel, Takuji Nakashima, Akira Take, Atsuko Matsumoto, Yoko Takahashi, Masahiro Ando, Haruko Takeyama
Summary: This study used Raman microspectroscopic imaging to investigate the correlation between avermectin production and morphological differentiation in Streptomyces avermitilis. The results showed distinct variations in the localization of secondary metabolites at different stages of morphological differentiation. Avermectin was detected in the later stages of differentiation under solid culture, but concentrated in the early stages under liquid culture. The chemical profiles of the mycelia also differed significantly depending on the culture condition.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Qing He, Wen Yang, Weiquan Luo, Stefan Wilhelm, Binbin Weng
Summary: This study introduces a rapid method using machine-learning-assisted Raman spectroscopic imaging to distinguish cancer cells from non-cancer cells, efficiently retrieving biomolecular information for cell line differentiation.
Review
Biochemistry & Molecular Biology
Jiang Qian, Xue Gao, Ya-Di Wang, Xue-Ling Li, Jun Hu, Jun-Hong Lu
Summary: Stem cells have shown great potential for tissue regeneration and repair due to their unlimited self-renewal and differentiation capabilities. However, the complex interactions between niches and stem cells, along with the limitations of current systems or methods, pose challenges to the multi-faceted applications of stem cells. Emerging evidence suggests that synchrotron infrared microspectroscopy may offer a promising non-invasive technology for stem cell research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Jiang Qian, Yadi Wang, Xueling Li, Junhong Lu
Summary: The stem cell microenvironment is crucial for regulating cell proliferation and differentiation. This study utilizes synchrotron radiation-based Fourier transform infrared microspectroscopy to examine the combined effects of physical and chemical factors on stem cell differentiation at the single-cell level. The results demonstrate the distinct effects of low-concentration lithium and Wnt5a on human mesenchymal stem cells in a polyvinyl alcohol (PVA) hydrogel, highlighting the important role of niche signals in the Wnt pathway. These findings emphasize the significance of the microenvironment in chemical-induced effects on stem cell differentiation and provide a label-free, noninvasive method to identify niche function in stem cell biology.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Environmental Sciences
Cheng Fang, Yunlong Luo, Xian Zhang, Hongping Zhang, Annette Nolan, Ravi Naidu
Summary: This study compares two decoding approaches for visualising microplastics and nanoplastics via Raman imaging. The first approach maps Raman intensity at characteristic peaks when standard spectra are available, while the second approach uses PCA to decode the spectrum matrix for complex samples without standard spectra. The PCA-based algorithm shows advantages in analysing and identifying microplastics from other materials.
Article
Cell Biology
Fide Sevgi, Eva M. Brauchle, Daniel A. Carvajal Berrio, Katja Schenke-Layland, Nicolas Casadei, Madhuri S. Salker, Olaf Riess, Yogesh Singh
Summary: Parkinson's disease is characterized by the formation of Lewy bodies in the brain, containing aggregated alpha-Synuclein. Raman imaging technology can detect alpha-Synuclein aggregates in colon tissues, showing promise as a useful tool in PD pathology research.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Environmental Sciences
Antonio Ragusa, Alessandro Svelato, Criselda Santacroce, Piera Catalano, Valentina Notarstefano, Oliana Carnevali, Fabrizio Papa, Mauro Ciro Antonio Rongioletti, Federico Baiocco, Simonetta Draghi, Elisabetta D'Amore, Denise Rinaldo, Maria Matta, Elisabetta Giorgini
Summary: The study revealed the presence of microplastic fragments in human placentas, with different shapes and possible origins from man-made coatings, paints, adhesives, etc.
ENVIRONMENT INTERNATIONAL
(2021)
Article
Nanoscience & Nanotechnology
Julia Marzi, Ellena Fuhrmann, Eva Brauchle, Verena Singer, Jessica Pfannstiel, Isabelle Schmidt, Hanna Hartmann
Summary: Raman imaging was successfully implemented in this study for molecular-sensitive investigations on bioprinted objects, enabling non-invasive and non-destructive evaluation of embedded cells and discrimination between different cell types and their molecular phenotypes. It is a valuable tool for quality control of bioprinted objects.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Raquel C. C. Laginha, Clara B. B. Martins, Ana L. C. Brandao, Joana Marques, M. Paula M. Marques, Luis A. E. Batista de Carvalho, Ines P. Santos, Ana L. M. Batista de Carvalho
Summary: In this study, the cytotoxic effects of a complex with two Pd(II) centers (Pd(2)Spm) on healthy and cancerous prostate human cell lines were evaluated and compared to cisplatin. Fourier Transform Infrared (FTIR) and Raman microspectroscopies were used to understand the molecular mechanisms of action of both drugs, revealing differences in their impact on DNA, lipids, and proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Spectroscopy
Martina Bonsignore, Sebastiano Trusso, Claudia De Pasquale, Guido Ferlazzo, Alessandro Allegra, Vanessa Innao, Caterina Musolino, Domenico Franco, Laura Maria De Plano, Salvatore Pietro Paolo Guglielmino, Fortunato Neri, Enza Fazio
Summary: In this study, micro-Raman spectroscopy was used to explore the spectral differences between different subsets of multiple myeloma plasma cells. The differences were analyzed through principal component analysis, revealing changes in protein and lipid contributions. This technology can be proposed as a diagnostic tool for rapid cell identification and assessment of intracellular biochemical changes in cancer cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Biophysics
Intan Rosalina Suhito, Yoojoong Han, Yong-Sang Ryu, Hyungbin Son, Tae-Hyung Kim
Summary: This study presents a novel optical method for ultra-fast and label-free characterization of cells, utilizing autofluorescence and Raman mapping integration. By using this technique, stem cell differentiation can be accurately and rapidly analyzed, making it highly useful for biomedical and clinical applications.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Spectroscopy
Nerea Iturrioz-Rodriguez, Daniele De Pasquale, Pietro Fiaschi, Gianni Ciofani
Summary: The study used Raman spectroscopy to compare GBM cells with healthy astrocytes, identifying that the region between 1000 and 1300 cm(-1) can effectively distinguish cancer cells from healthy cells. The model achieved an average accuracy of 92.5% in discriminating cancer cells from healthy cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Environmental Sciences
Yunlong Luo, Fangjie Qi, Christopher T. Gibson, Yongjia Lei, Cheng Fang
Summary: This research utilizes Raman imaging to characterize microplastics and nanoplastics released from a dish sponge, identifying the particles as mainly Nylon PA6 and polyethylene terephthalate. The use of principal component analysis enhances the signal-noise ratio and enables reliable capture and visualization of released microplastics and nanoplastics.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Spectroscopy
Yubin Zhao, Yoshinori Yamaguchi, Chenchen Liu, Mingda Li, Xiaoming Dou
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2019)
Article
Spectroscopy
Yubin Zhao, Yoshinori Yamaguchi, Yi Ni, Mingda Li, Xiaoming Dou
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2020)
Article
Chemistry, Analytical
Zhenqing Li, Pengbo Zhang, Bo Yang, Jiahui Liu, Heqi Xi, Dawei Zhang, Yoshinori Yamaguchi
Summary: The researchers proposed a high throughput and low-cost DNA concentration system based on fluorescence technology, which can measure the concentration of 24 DNA samples within 100 seconds by analyzing the self-luminescence of individual solutions in DNA test kit. This system provides a simple way for DNA concentration determination and is suitable for labs in third world countries.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Spectroscopy
Zh. E. Ozbekova, A. A. Abdyldaev, A. A. Kulmyrzaev
Summary: This study compared two drying methods for cow and yak muscles, finding that freeze drying caused less discoloration. Additionally, analysis of fluorescence spectra allowed for accurate prediction of chemical composition and color characteristics of the muscles.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Leo Mandic, Ivan Ljubic, Iva Dzeba
Summary: In this study, a combined experimental and computational approach was used to investigate the photoexcitation and photodegradation mechanisms of Doxazosin (DOX). The results provide valuable insights into the primary events following the photoexcitation of DOX and its potential applications.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Xiufeng Wang, Yao Jin, Wenhui Ai, Siqi Wang, Zhiqing Zhang, Ting Zhou, Fang Wang, Guodong Zhang
Summary: This study successfully synthesized near infrared fluorescence Ag2S quantum dots (QDs) and developed a dual-mode sensor for sulfide anion using the excellent oxidase-like characteristics of manganese dioxide (MnO2) nanosheets. The sensor showed a wider detection range, higher sensitivity, and shorter reaction time, making it suitable for highly selective detection of sulfide in different concentration ranges.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Yafei Li, Yanming Ma, Chuantao Zheng, Di Yu, Lien Hu, Shuo Yang, Fang Song, Yadan Li, Shuanghai Liu, Zhanrui Zhang, Yu Zhang, Yiding Wang, Frank K. Tittel
Summary: To effectively monitor gas explosion and coal spontaneous combustion in coal mine, an intrinsically safe and explosion-proof dual-gas sensor system was developed for methane (CH4) and carbon monoxide (CO) measurement. The system achieved different measurement ranges using laser scanning and gas cells, and improved stability and accuracy through gas pre-treatment and temperature compensation algorithm.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Pei-Rong Chen, Li-Kang Chu
Summary: In this study, the hydrates of glyoxal were investigated using infrared absorption spectrometry. The results showed that at low concentrations, glyoxal mainly exists as monomeric dihydrate. These findings provide suitable detection windows for further research on the roles of glyoxal and its hydrates in atmospheric and aerosol chemistry, as well as the relevant reaction kinetics.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Nichole O'Neill, Thamires A. Lima, Fabio Furlan Ferreira, Nicolas J. Alvarez, Reinhard Schweitzer-Stenner
Summary: This study determines the main fibril axis of GHG gel-forming fibrils using various techniques and compares the results with simulated data. The analysis suggests that the hydrophobic xz-surfaces of GHG fibrils could be a good target for the adsorption of hydrophobic drugs.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Mengyuan Liu, Hanchuang Zhu, Yikun Fang, Caiyun Liu, Xinke Li, Xiaohui Zhang, Lixue Ma, Kun Wang, Miaohui Yu, Wenlong Sheng, Baocun Zhu
Summary: ABHS is an efficient fluorescent probe that can accurately and sensitively detect Al3+ with strong resistance to interference. It also demonstrates excellent detection and imaging capabilities in complex real samples.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Ahmed S. El-Shafie, Evana Rahman, Yasser Gadelhak, Rehab Mahmoud, Marwa El-Azary
Summary: The recycling of waste mandarin peels into biochar (MRBC) has been used as a high performance and cost-effective adsorbent for treating polluted wastewater effluents. Batch adsorption studies were conducted to analyze the adsorption competency of MRBC on two dyes, methylene blue (MB) and basic fuchsin (BF), either in individual solutions or binary combinations. The results showed that MRBC was capable of effectively removing a high percentage of both dyes. However, the adsorption capacity decreased when the dyes were combined. The cost estimation of MRBC production and wastewater treatment indicated that both were relatively low.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Anindita Bhatta, Jahnabi Upadhyaya, Dipak Chamlagai, Lincoln Dkhar, Pynskhemborlang T. Phanrang, Mohan Rao Kollipara, Sivaprasad Mitra
Summary: Derivatives of thiazole-pyrazole fused benzo-coumarin compounds were synthesized and their photophysical properties were investigated. The synthesized coumarin compounds showed potential as therapeutic agents for Alzheimer's disease, inhibiting acetylcholinesterase activity. The presence of human serum albumin was found to affect the inhibition activity.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Yongwei Duan, Quancheng Liu, Yiju Zhu, Qi Zhang, Xiaohui Duan, Hu Deng, Liping Shang
Summary: In this study, the phase transition of CL-20 was observed using terahertz spectroscopy, and quantum chemical calculations were employed to analyze the vibrations. The results indicated changes in the vibrations of CL-20 before and after phase transition.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Yuefeng Gao, Sai Xu, Baojiu Chen
Summary: This study synthesized Cs2NaInCl6 nanocrystals co-doped with Sb3+ and Tb3+ ions as probes for copper ions detection in lubricating oil. The introduction of Sb3+ effectively reduced the band gap of the host material and enabled energy transfer pathway for Tb3+ emission. The doped Tb3+ ions resulted in the suppression of emission due to electron transfer. The Cs2NaInCl6: 2.5 %Sb3+, 40 %Tb3+ NCs exhibited superior sensitivity and selectivity for copper ions detection.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Yu Liu, Yue Zhang, Changyao Liu, Ce Wang, Baocai Xu, Li Zhao
Summary: A highly sensitive detection platform for heparin was developed using a cationic fluorescent dye (cresyl violet acetate) as a fluorescence probe. The platform demonstrated high selectivity towards heparin and achieved detection in both HEPES solution and serum.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Lopamudra Roy, Nivedita Pan, Susmita Mondal, Ria Ghosh, Md. Nur Hasan, Neha Bhattacharyya, Soumendra Singh, Kallol Bhattacharyya, Arpita Chattopadhyay, Samir Kumar Pal
Summary: The study focuses on the interaction between reactive oxygen species (ROS) and a spectroscopic probe called Rose Bengal (RB), encapsulated in nanoscopic sodium dodecyl sulphate (SDS) micelles and entrapped in microscopic nylon 66 solid matrix. The research demonstrates efficient interaction between ROS and RB-SDS, and investigates the mechanism of hydroxyl radicals generation. Based on these findings, a prototype device utilizing RB embedded in a nylon thin film for quantification of ROS in extracellular fluids and food materials was developed.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Xiaosong Sun, Qihao Cui, Wenyue Dong, Qian Duan, Teng Fei
Summary: Conjugated porous polymers (CPPs) are promising sensing materials and their application in aqueous media is limited. In this study, we synthesized CPPs with porous structure and prepared nanoparticles, enabling efficient photoluminescence sensing of nitroaromatic explosives in aqueous phase.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Spectroscopy
Maria Cristina Caggiani, Germana Barone, Paolo Mazzoleni
Summary: Raman spectroscopy is commonly used for studying glassy materials in cultural heritage, but it is more difficult to interpret the spectra and apply the technique with portable instruments. In contrast, diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) can be used in archaeometric investigations as it is portable and non-invasive. However, there is limited application of this technique to historical glasses. This exploratory work demonstrates the potential of DRIFTS, in combination with portable X-ray Fluorescence (pXRF) and EDS microanalyses, for studying the composition and alteration of glass samples in cultural heritage.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)