Article
Chemistry, Medicinal
Shufen He, Peishan Li, Jingxuan Wang, Yanzhu Zhang, Hongmei Lu, Liufei Shi, Tao Huang, Weiyan Zhang, Lijian Ding, Shan He, Liwei Liu
Summary: In this study, the distribution of secondary metabolite biosynthetic gene clusters (SMBGCs) in Hahella was examined, and a potential source of bioactive compounds was identified. A strain named NBU794 was isolated from mangrove soil and found to contain multiple SMBGCs and bioactive compounds, including prodigiosin and a novel compound called 2-methyl-3-heptyl-prodiginine. These compounds showed antimicrobial activity, making NBU794 a promising source for prodigiosin.
Article
Plant Sciences
Anza-Tshilidzi Ramabulana, Paul A. Steenkamp, Ntakadzeni E. Madala, Ian A. Dubery
Summary: Plant cell culture can be manipulated using plant growth regulators to induce metabolic changes for the production of specialized metabolites. This study investigated different combination ratios of PGRs to induce metabolic changes in Bidens pilosa callus, revealing distinct metabolomic profiles dominated by chlorogenic acids. While organogenesis was not achieved, the results demonstrate the potential of using exogenous PGRs to manipulate plant metabolism for in vitro production of specialized metabolites.
Article
Plant Sciences
Emmanuel Cabanas-Garcia, Carlos Areche, Yenny Adriana Gomez-Aguirre, Jorge Borquez, Ruben Munoz, Francisco Cruz-Sosa, Eugenio Perez-Molphe Balch
Summary: Plant cell, tissue, and organ culture have been utilized as a powerful technology for biomass production and secondary metabolite biosynthesis. This study presents an efficient method for inducing friable callus in the medicinal cactus Coryphantha macromeris, analyzing its kinetic behavior and phytochemical profile. The research achieved high biomass production and identified multiple metabolites, contributing to the understanding of tissue culture and potential applications of the cactus species.
SOUTH AFRICAN JOURNAL OF BOTANY
(2021)
Article
Biotechnology & Applied Microbiology
Ling Yang, Kunlin Wu, Shengxiang Qiu, Honglin Cao, Shulin Deng
Summary: This study successfully established a regeneration system for the multifunctional shrub Rhodomyrtus tomentosa by inducing callus from tender leaves and stems. The induced callus was light yellow-green and rigid, with granules, and 93.33% of it differentiated into adventitious shoots within 60 days. The optimal shoot proliferation medium was 1/2 MS medium with 1 mg L-1 6-BA and 0.1 mg L-1 NAA, and the highest regeneration response rate (86.67%) was obtained under high temperature and high light intensity conditions (26 degrees C, 120 mu mol m(-2)s(-1)). Adventitious roots showed the highest rate of rooting (100%) on PGR-free 1/2 MS medium. Over 90% of plantlets survived when transferred to a substrate of sand/soil (1:2 ratio) within 2 months.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Pharmacology & Pharmacy
Laura Gence, Danielle Fernezelian, Matthieu Bringart, Bryan Veeren, Armelle Christophe, Francois Brion, Olivier Meilhac, Jean-Loup Bascands, Nicolas Diotel
Summary: This study investigates the biological properties of the traditional medicinal plant Hypericum lanceolatum Lam., revealing its antioxidant, pro-regenerative, anti-lipid accumulation, and pro-neurogenic effects. The results support the potential use of this plant as an alternative treatment for metabolic disorders.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Agronomy
Yasmeen Saleem, Muhammad Zaka Emad, Aamir Ali, Shagufta Naz
Summary: This study evaluated the response of different plant growth regulators on callus induction and regeneration in three sugarcane genotypes. The best callus response was obtained with the combination of 2,4-D and Kinetin, while Kinetin and BAP were found to be the most effective for shoot induction, and NAA and IBA were used for root induction. The results provide valuable information for optimizing tissue culture protocols for sugarcane.
Article
Plant Sciences
K. P. Sharanya, K. G. Ajith Kumar, Parvathy S. Nair
Summary: This study investigated the embryogeny process of Syzygium zeylanicum and found that the species has poor seed regeneration ability. The study also analyzed hormonal and physiological changes, and discovered the low desiccation tolerance of the seeds.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Food Science & Technology
Roberta De Bellis, Laura Chiarantini, Lucia Potenza, Andrea Gorassini, Giancarlo Verardo, Rossella De Marco, Leila Benayada, Vilberto Stocchi, Maria Cristina Albertini, Daniele Fraternale
Summary: This study developed an innovative method for extracting secondary metabolites from quince callus cultures and analyzed its composition and activities using various techniques. The extract exhibited good antioxidant activity and strong inhibition of alpha-glucosidase and lipase, indicating its potential for developing nutraceutical approaches for managing hyperglycemia and dyslipidemia.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Agricultural Engineering
N. P. Makunga, E. Hall, M. A. Stander
Summary: A biotechnological method for producing mesembrine alkaloids from Sceletium tortuosum has been developed using in vitro microshoot and callus suspension cultures, offering an alternative source for clinical herbal formulations.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Food Science & Technology
Xuan Zeng, Yuying Zheng, Yan He, Wei Peng, Weiwei Su
Summary: Naringin is a dietary flavonoid glycoside with multiple bioactivities. This study developed a rapid and sensitive method for simultaneous determination of ten flavonoid metabolites of naringin in rat urine. The results revealed the visible individual difference and low urinary recovery of flavonoid metabolites in the excretion of naringin, which may be helpful for further study to understand the in vivo behavior and action mechanism of naringin.
Article
Food Science & Technology
Fulya Tasci, Hale Secilmis Canbay, Mahmut Doganturk
Summary: The study found antibiotic residues in non-compliant milk samples, suggesting that not all milk producers adhere to good veterinary practices.
Article
Plant Sciences
Jauhar Ali, Katrina Leslie C. Nicolas, Shahana Akther, Azerkhsh Torabi, Ali Akbar Ebadi, Corinne M. Marfori-Nazarea, Anumalla Mahender
Summary: The study showed that the improved anther culture medium A1 significantly enhanced callus induction frequency of japonica rice type, indica rice type, and hybrids of indica and japonica cross. Furthermore, A1 medium greatly improved the green plantlet regeneration efficiency of the hybrids CXY6, CXY24, and Y2, demonstrating its potential for producing doubled haploid rice plants for different breeding programs.
Article
Plant Sciences
Fernanda R. R. Castro-Moretti, Jean-Christophe Cocuron, Humberto Castillo-Gonzalez, Efrain Escudero-Leyva, Priscila Chaverri, Oliveiro Guerreiro-Filho, Jason C. C. Slot, Ana Paula Alonso
Summary: The aim of this study was to develop a high-throughput workflow to identify and quantify plant polyphenols. A method was optimized for extracting over 40 families of phytochemicals and a high-throughput metabolomic platform was developed to identify and quantify 184 polyphenols in 15 min.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Energy & Fuels
P. R. Dwarakanath, K. Abinaya, K. Nagasathya, S. Meenakumari, Subash C. B. Gopinath, Pachaiappan Raman
Summary: This study focuses on the extraction of secondary metabolites from P. perlatum lichen, which has antioxidant and antibacterial activity. The methanol extract of P. perlatum exhibits the highest antibacterial activity, while all extracts show potential for radical scavenging.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Agriculture, Multidisciplinary
Guo Lin-hui, Ge Da-peng, Ren Yuan, Dong Jian-mei, Zhao Xue-qing, Liu Xue-qing, Yuan Zhao-he
Summary: Secondary metabolites in pomegranates are closely related to nutritional quality and health functions. A study on wild and cultivated pomegranate plants in China revealed significant differences in flavonoid and tannin contents among different accessions. Latitude and altitude were identified as main environmental factors affecting the content of secondary metabolites. Over 200 secondary metabolites were identified in pomegranates, with 90 differential metabolites identified in two specific parts of the plant. This study provides valuable insights for utilizing wild pomegranate resources and breeding programs in China.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Plant Sciences
Wanli You, Jinglin Zhang, Xueyin Ru, Feng Xu, Zhengguo Wu, Peng Jin, Yonghua Zheng, Shifeng Cao
Summary: This study investigated the effect of calcium chloride (CaCl2) treatment on GABA accumulation in fresh-cut cantaloupe and the underlying mechanisms. The results showed that CaCl2 treatment increased GABA content and the activities of GAD and SSADH enzymes, while reducing glutamate content and GABA-T activity. Additionally, CaCl2 treatment upregulated the expressions of CmCML11 and CmCAMTA5, as well as several GABA shunt genes, through the transcriptional activation by CmCAMTA5. Furthermore, the interaction between CmCML11 and CmCAMTA5 enhanced the transcriptional activation of GABA shunt genes. Overall, this study reveals that CaCl2 treatment promotes GABA accumulation in fresh-cut cantaloupe through the combined effect of CmCML11 and CmCAMTA5 in regulating the expressions of GABA shunt genes.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Min Wang, Yupeng Wang, Xiaohui Wang, Guangwei Wei, Huiyi Yang, Xi Yang, Tinghai Shen, Huijie Qu, Sheng Fang, Ziming Wu
Summary: This study identified the high nitrogen use efficiency (NUE) black sesame variety 17-156 and analyzed its underlying physiological and molecular mechanisms. The results showed that 17-156 possesses a sophisticated nitrogen metabolizing machinery to uptake and assimilate higher quantities of inorganic nitrogen, simultaneously improving carbon metabolism and growth. Many important genes were up-regulated in 17-156 under high nitrogen condition. Additionally, 38 potential candidate genes were identified for future studies to improve sesame's NUE. These findings provide valuable resources for understanding the regulatory network of nitrogen metabolism and developing sesame cultivars with improved NUE.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
He Dong, Chongmei Xu, Chengtao Zhang, Li Zhang, Yaqin Yao, Suiqi Zhang
Summary: The study found that short cells in maize leaves not only improve leaf mechanical support and photosynthetic performance, enhance drought resistance, but also participate in stomatal regulation.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Na Liu, Wenyan Shang, Mengxin Guan, Jibin Xiao, Guangxiang Tian, Baozhan Ma, Wenjing Shang, Xu Li, Shijia Zhao, Chuang Li, Kun Cheng, Wenming Zheng
Summary: This study cloned the full-length cDNA sequence of TaSPX3 gene in wheat and found that TaSPX3 responds to low phosphorus stress in multiple wheat genotypes. Overexpressing TaSPX3 can alleviate phosphorus deficiency symptoms and promote plant growth in Arabidopsis. The study also revealed the interaction of TaSPX3 with other genes related to the phosphorus starvation signaling pathway.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Kongyuan Wu, Lizhen Wang, Zihan Wu, Ziqing Liu, Zengfei Li, Jun Shen, Shengjie Shi, Hong Liu, Christopher Rensing, Renwei Feng
Summary: Selenium (Se) can reduce uptake and translocation of cadmium (Cd) in plants by regulating root morphology. This study investigated the effects of Se(IV) on root exudates, root morphology, root endogenous hormones, and Cd uptake efficiency in rice under Cd stress. The results showed that Se(IV) significantly reduced Cd concentrations in shoots and roots, and decreased Cd uptake efficiency via root hairs. Se(IV) also affected root morphology, root exudates, and the synthesis of hormones like IAA and JA. However, transcriptome analysis revealed no upregulated differentially expressed genes (DEGs) in IAA synthesis.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Di Wang, Huaifang Zhang, Xuefei Hu, Haizhen Zhang, Shuang Feng, Aimin Zhou
Summary: This study identified a cell number regulator gene called SlCNR8 in willow, which enhances resistance to trace metals in transgenic poplar seedlings. SlCNR8 reduces Cd uptake and accumulation, and can be used as a candidate gene for genetic improvement of phytostabilisation of trace metals.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)