Article
Plant Sciences
Jia-Ning Liu, Zi-Yi Yang, Jin-Hui Zheng, Ying Xu, Lei Fang
Summary: Two new monoterpenoid indole alkaloids, alstoscholines A and B, were isolated from Alstonia scholaris stems, along with three known compounds. The structures and absolute configurations of the new compounds were elucidated using HRMS and NMR spectroscopic data and computational calculation methods. Compounds 1 and 2 showed moderate inhibitory effects on LPS-induced nitric oxide (NO) production in RAW 264.7 macrophages.
PHYTOCHEMISTRY LETTERS
(2023)
Article
Chemistry, Organic
Guanqun Zhan, Fuxin Zhang, Kailing Yang, Tao Yang, Ruixi Zhou, Xueqing Ma, Nan Wang, Zengjun Guo
Summary: A new compound and four known compounds were isolated from Alstonia scholaris. The new compound has a unique carbon skeleton and shows potential anti-inflammatory activity.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Article
Chemistry, Organic
Hao-Fei Yu, Cai-Feng Ding, Lan-Chun Zhang, Xin Wei, Gui-Guang Cheng, Ya-Ping Liu, Rong-Ping Zhang, Xiao-Dong Luo
Summary: Alstoscholarisine K, a novel indole alkaloid with a unique octacyclic architecture, showed significant antibacterial activities by targeting cell membranes, and was specifically found in gall-infected leaves of Alstonia scholaris.
Article
Plant Sciences
Rui Guo, Jian-Hua Shang, Rui-Han Ye, Yun-Li Zhao, Xiao-Dong Luo
Summary: This study confirms the potential therapeutic effect of alkaloids from A. scholaris on chronic glomerulonephritis (CGN). By regulating four key proteins (HRAS, CDK2, HSP90AA1, and KDR) of the PI3K-Akt pathway, TA and two major bioactive components (vallesamine and 19-epi-scholaricine) can exert protective effects against ADR-induced CGN in the MPC5 cells model.
Article
Biochemistry & Molecular Biology
Fuxin Zhang, Kailing Yang, Huanhuan Liu, Tao Yang, Ruixi Zhou, Xinxin Zhang, Guanqun Zhan, Zengjun Guo
Summary: Seven undescribed monoterpene indole alkaloids, alstoscholarinines A-G, were isolated from the branches of Alstonia scholaris (L.) R. Br., along with nineteen known alkaloids. The structures of the undescribed alkaloids were determined through spectroscopic analysis, ECD calculation, and X-ray diffraction analysis. Among the alkaloids, alstoscholarinine A is a unique and unreported monoterpene indole alkaloid with three nitrogen atoms, featuring a compact hexacyclic system and a unique oxazolidine ring. Some of the isolated alkaloids showed significant vasorelaxant activities, with the akuammicine-type alkaloids exhibiting better activities compared to other framework types.
Article
Chemistry, Organic
Jing Xie, Xueyi Zou, Chenchen Sang, Min Song, Qiuling Chen, Jian Zhang
Summary: Three new monoterpenoid indole alkaloids were isolated from Alstonia rostrata, with compound 2 possessing a unique hexacyclic ring system and exhibiting potent vasorelaxant activity, while compound 5 showed moderate acetylcholinesterase inhibitory activity.
TETRAHEDRON LETTERS
(2021)
Article
Plant Sciences
Yun-Li Zhao, Shi-Biao Pu, Yan Qi, Bai-Fen Wu, Jian-Hua Shang, Ya-Ping Liu, Di Hu, Xiao-Dong Luo
Summary: The study found that total alkaloids have significant anti-pulmonary fibrosis effects against bleomycin-induced lung fibrosis, possibly by reducing collagen deposition through the TGF-β/MMP-1 pathway. Additionally, indole alkaloids may be the main active ingredients of total alkaloids.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Chemistry, Medicinal
Fuxin Zhang, Huanhuan Liu, Kailing Yang, Tao Yang, Ruixi Zhou, Rongkun Miao, Guanqun Zhan, Zengjun Guo
Summary: New natural compounds were discovered from the branches of Alstonia scholaris, and a possible biosynthetic pathway for a new type of monoterpene alkaloids was proposed for the first time. Some compounds showed significant vasorelaxant activity, indicating their potential as lead compounds for vasodilators.
Article
Chemistry, Physical
Yu-qi Li, Jing Cai, Jia-xin Cheng, Sheng-zhi Su, Toshihiro Akihisa, Wen-yuan Liu, Feng Feng, Jie Zhang
Summary: This study investigated the chemical constituents of Alstonia mairei and isolated twenty-four monoterpenoid indole alkaloids. Four new compounds were discovered. The structures of these compounds were characterized using various techniques. The cytotoxic activities of the isolated compounds were evaluated, and several compounds demonstrated potent inhibitory activities against A549 lung cancer cells.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Plant Sciences
Bin-Yuan Hu, Yun-Li Zhao, Yuan Xu, Xiao-Na Wang, Xiao-Dong Luo
Summary: The leaves of Alstonia scholaris yielded twelve lupanes, including three new compounds. The structures of these compounds were determined through spectroscopic analysis and ECD calculation. Among them, compound 1 displayed a rare seco A-ring skeleton and lactone formation, while compounds 2 and 3 showcased the phytochemical diversity and novelty with their unique lupane skeletons. Pharmacologically, compounds 4, 7, and 10 exhibited significant reduction of serum uric acid levels in mice.
Article
Biochemistry & Molecular Biology
Qi-Ming Pan, Yu-Huan Li, Jian-Jun Zhang, Yong Li, Shuang-Gang Ma, Shi-Shan Yu
Summary: In this study, several undescribed monoterpenoid indole alkaloids were isolated from Strychnos cathayensis. (17S)-12-hydroxy-11-methoxydiaboline's absolute configuration was determined for the first time. Some of the isolated alkaloids showed potential analgesic and antiviral activities.
Article
Chemistry, Organic
Bin-Yuan Hu, Yun-Li Zhao, Deng-Sen Xiong, Ying-Jie He, Zhong-Shun Zhou, Pei-Feng Zhu, Zhao-Jie Wang, Yong-Liang Wang, Li-Xing Zhao, Xiao-Dong Luo
Summary: Two novel rearranged triterpenoids were isolated from Alstonia scholaris, representing new subtypes of pentacyclic triterpenoids with unique ring systems. Their structures were determined by various methods and they exhibited potent antihyperuricemic bioactivity.
Article
Chemistry, Organic
Guanqun Zhan, Fuxin Zhang, Kailing Yang, Tao Yang, Ruixi Zhou, Wenwen Chen, Jingwei Zhang, Xinxin Zhang, Zengjun Guo
Summary: Unprecedented rearranged monoterpene indole alkaloids A and B were isolated from Alstonia scholaris. They exhibit unique structures and activities.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Article
Chemistry, Organic
Peng Zou, Hongjian Yang, Jian Wei, Taimin Wang, Hongbin Zhai
Summary: The first asymmetric total synthesis of three picrinine-type akuammiline alkaloids has been achieved, involving an efficient acid-promoted oxo-bridge ring-opening, an unusual Dauben-Michno oxidation, and a nickel-mediated reductive Heck reaction to forge the [3.3.1]-azabicyclic core.
Article
Chemistry, Multidisciplinary
Bin-Yuan Hu, Yun-Li Zhao, Zhong-Shun Zhou, Yan-Yan Zhu, Xiao-Dong Luo
Summary: Two unique windmill-like aziridine-containing indole alkaloids with unprecedented ring systems and an unusual core were isolated from Alstonia scholaris. The structures of these novel compounds were established through spectroscopy, X-ray diffraction, and electronic circular dichroism calculations. The compounds displayed significant anti-inflammatory activity in vitro and alleviated LPS-induced acute lung injury in mice.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Applied
Akash R. Gohil, Sunil K. Deshmukh, Vaishali Bhattacharya, Rahul Lavhale, Shilpa Verekar, Abhijeet S. Kate
Summary: The chemical characterization of ethyl acetate extract of Exophiala sp. led to the isolation of three compounds, including a new isocoumarin named exophiarin. The structure was elucidated using 1D, 2D NMR and HRMS data, and the compound showed moderate improvement in glucose uptake activity in rat skeletal muscle cell line L6 when tested.
NATURAL PRODUCT RESEARCH
(2021)
Article
Chemistry, Analytical
Prakash Niguram, Ashutosh Goswami, Abhijeet S. Kate
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2020)
Article
Materials Science, Characterization & Testing
K. Sattaiah Naidu, Abhijeet S. Kate, Vikas Kshirsagar, R. Ganeshan, Tukaram Gunale, Bing Zhou, Samir Anapat, Yusuf Sulub, Arun Kumar, Narayana Rao
Article
Plant Sciences
Ruchi Singh, Mini Dhiman, Arvind Saklani, C. Immanuel Selvaraj, Abhijeet S. Kate
Summary: A new flavanone glycoside and three known flavonoids were isolated from an endemic plant in the Western Ghats of India. The structure of the new compound was elucidated using experimental data, and it showed improvement in glucose uptake in vitro.
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
(2022)
Article
Biochemical Research Methods
Disha Thakkar, Amit Kumar Sahu, Rajeshwari Rathod, Pinaki Sengupta, Abhijeet S. Kate
Summary: The study found that the combined administration of grapefruit juice and pomegranate juice increased the systemic exposure of Brexpiprazole and affected its pharmacokinetic parameters, while tomato juice had no significant effect.
BIOMEDICAL CHROMATOGRAPHY
(2021)
Article
Immunology
Sanmoy Pathak, Abhijeet Gokhroo, Ashim Kumar Dubey, Shamik Majumdar, Souradeep Gupta, Asha Almeida, Girish B. Mahajan, Abhijeet Kate, Prabhu Mishra, Rajiv Sharma, Sanjay Kumar, Ram Vishwakarma, Arun Balakrishnan, Hanudatta Atreya, Dipankar Nandi
Summary: Sesquiterpene lactone 7-hydroxy frullanolide (7HF) inhibits CD4+ T cell and peritoneal macrophage responses through regulating cell cycling and intracellular calcium levels, exhibiting anti-inflammatory potential in reducing immune responses.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Chaitrali Shevkar, Ashwini Armarkar, Ramani Weerasinghe, Kasun Maduranga, Komal Pandey, Santosh K. Behera, Kiran Kalia, Priyani Paranagama, Abhijeet S. Kate
Summary: Lichens have been used in traditional medicines worldwide for wound healing and treating skin disorders and respiratory diseases. In this study, the researchers investigated 31 endolichenic fungi isolated from 32 lichens found on mangrove plants in Sri Lanka. They identified three cytotoxic molecules from one of the fungi, Talarotnyces pinophilus, and evaluated their anticancer potential against various cancer cell lines.
INDIAN JOURNAL OF MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Chaitrali Shevkar, Pranali Pradhan, Ashwini Armarkar, Komal Pandey, Kiran Kalia, Priyani Paranagama, Abhijeet S. Kate
Summary: Fungi can produce various scaffolds with unique biological activities, but so far, no molecule derived from fungi has become an anti-cancer drug on the market. Every year, numerous cytotoxic molecules of fungal origin are published, making it necessary to critically analyze these compounds to identify the potent ones. A review published in 2014 mentioned the best cytotoxic fungal metabolites and their status in drug development. In this report, we included 176 cytotoxic molecules isolated from fungi after 2014 and categorized them based on their potencies. Emphasis was given to discuss the 42 molecules that showed IC50 values below 1 mu M. This review provides potent fungal scaffolds that can be prioritized as drug candidates for cancer therapeutics.
CHEMISTRY & BIODIVERSITY
(2022)
Article
Chemistry, Multidisciplinary
Ramani H. Weerasinghe, Chaitrali D. Shevkar, Kasun Maduranga, Komal H. Pandey, Renuka N. Attanayake, Abhijeet S. Kate, Gothamie Weerakoon, Santosh K. Behera, Kiran S. Kalia, Priyani A. Paranagama
Summary: Bioassay-guided fractionation of the ethyl acetate extract from the endolichenic fungus Phanerochaete sordida led to the isolation of a bioactive compound. The compound exhibited high antioxidant activity, moderate anti-inflammatory and antibacterial activity, and moderate tyrosinase inhibition and cytotoxicity. In vitro and in silico experiments confirmed the bioactivities and drug-like properties of the compound.
JOURNAL OF CHEMISTRY
(2022)
Article
Chemistry, Applied
Debanjan Chatterjee, Nazmina Vhora, Ashutosh Goswami, Aishwarya Hiray, Alok Jain, Abhijeet S. Kate
Summary: The study analyzed small molecules from nature with antidiabetic properties and verified the activity of alpha-mangostin through in-vitro and in-silico studies. This screening strategy provides a rapid method for identifying effective natural products.
NATURAL PRODUCT RESEARCH
(2023)
Article
Biochemical Research Methods
Prakash Niguram, Abhijeet S. Kate
Summary: This study comprehensively reports the in vitro and in vivo metabolism of ART, identifying 15 metabolites with 11 novel metabolites reported for the first time. The use of HILIC proved to be efficient in separating various metabolites, providing insights into the disposition of ART and its congeners.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2022)
Article
Chemistry, Medicinal
Monika Sharma, Fehmina Mushtaque Malim, Ashutosh Goswami, Nishant Sharma, Sai Sowmya Juvvalapalli, Sayan Chatterjee, Abhijeet S. Kate, Amit Khairnar
Summary: The study evaluated the potential of Swertiamarin (SWE) as a treatment for Parkinson's disease (PD). The results demonstrated that SWE can reduce inflammatory response and protect neurons from damage. Therefore, SWE may be a promising adjunct therapy for PD.
ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Ashutosh Goswami, Bhakti Nanaware, Abhijeet S. S. Kate
Summary: Swertiamarin has the potential to treat metabolic illnesses and Parkinson's disease, and its hepatoprotective and anti-aging effects are evident. Researchers have developed a four-step technique to enrich Swertiamarin in the extract, resulting in a purity of 97%. This method resolves the issues of traditional chromatography techniques and enhances the market value and research prospects of Swertiamarin.
SEPARATION SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Medicinal
Monika Sharma, Fehmina Mushtaque Malim, Ashutosh Goswami, Nishant Sharma, Sai Sowmya Juvvalapalli, Sayan Chatterjee, Abhijeet S. Kate, Amit Khairnar
Summary: Parkinson's disease (PD) is a progressive neurodegenerative disease with no permanent cure. The accumulation of toxic alpha-synuclein and neuronal inflammation are key factors in its pathology. Swertiamarin (SWE), a natural product, has been found to have anti-inflammatory effects and a potential reversal effect on alpha-syn accumulation. In this study, SWE showed anti-inflammatory activity in glial cells and neuroprotective effects in a mouse model of PD. Further research is needed to fully understand the mechanisms involved.
ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Preeti Bharatiya, Pooja Rathod, Aishwarya Hiray, Abhijeet S. Kate
Summary: Natural products play a vital role in treating various diseases, with fungi being a beneficial source due to their production of antibiotics, food colorants, enzymes, and nutritious food. However, redundant isolation of metabolites hinders discovery rates. To trigger biosynthetic genes in fungi, induction strategies beyond standard conditions are necessary. Advancements in computational tools aid in connecting gene clusters and their metabolite production, leading to the identification of numerous cryptic metabolites.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
