Journal
OPTIK
Volume 206, Issue -, Pages -Publisher
ELSEVIER GMBH
DOI: 10.1016/j.ijleo.2020.164292
Keywords
HR-HPV; FT-IR spectroscopy; PCA-LDA; Cervical exfoliated cells; Screening
Categories
Funding
- National Natural Science Foundation of China (NSFC) [61801042, 61771067, 61765014]
- State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia [SKL-HIDCA-2018-29]
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Persistent high-risk human papillomavirus (HR-HPV) infection is leading cause for the occurrence of cervical cancer, and timely detection and early treatment of HR-HPV infection can effectively reduce incidence of cervical cancer. In this study, a rapid and non-invasive method for detecting HR-HPV was proposed by using Fourier transform infrared (FT-IR) spectra of cervical exfoliated cells combined with multivariate analysis. A total of 100 spectra were recorded from 50 HR-HPV positive patients and 50 normal subjects. The obvious difference in infrared spectrum between the two groups was mainly shown at 1042 cm(-1) (mucin), 1246 cm(-1) (amide III), 1396 cm(-1) (proteins), 1543 cm(-1) (amide II), 1651 cm(-1) (amide I), 2361 cm(-1) (CO2), 2928 cm(-1) (lipids), and 3294 cm(-1) (amide A). Then, a principal component analysis-linear discriminant analysis (PCA-LDA) diagnostic model was developed and applied on the FT-IR spectra of normal samples as well as HR-HPV positive patients, and satisfactory classification results were obtained. The diagnostic accuracy, specificity, and sensitivity were 98 %, 98 %, and 98 %, respectively. Furthermore, the area under the receiver operating characteristic (ROC) curve (AUC) was 0.997, which could further demonstrate the feasibility of the PCA-LDA model. Therefore, our exploratory work shows that the combination of FT-IR spectroscopy and PCA-LDA model has great potential for HR-HPV screening.
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