Article
Chemistry, Analytical
Sophie Catanese, Raoul K. Khanna, Antoine Lefevre, Hugo Alarcan, Pierre-Jean Pisella, Patrick Emond, Helene Blasco
Summary: To apply metabo-lipidomics in ophthalmological and systemic diseases, it is necessary to standardize the preanalytical and analytical steps in human tears. This study compared different methods for each step of the workflow and found that methanol/water extraction provided the best results for metabolomics, while Folch extraction was superior for lipidomics. The use of microcapillary glass tube for sampling was superior to Schirmer strip for metabolomics. A volume of 5 mu L provided satisfactory metabo-lipidomic coverage. These findings will contribute to improving the metabo-lipidomic workflow in human tears for the identification of new biomarkers.
Article
Biochemical Research Methods
Chris Weise, Hannes Westphal, Rico Warias, Detlev Belder
Summary: Here, a miniaturized chip-based HPLC approach coupled with electrospray ionization mass spectrometry is introduced, which utilizes temperature to achieve high-speed separations. With the advantages of low thermal mass of the microfluidic chip and pre-heated mobile phase to form an electrospray, isothermal and temperature-programmable operations were conducted to separate pesticides in methanol and ethanol-containing eluents in less than 20 s.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Kelly L. Pereira, Martyn W. Ward, John L. Wilkinson, Jonathan Brett Sallach, Daniel J. Bryant, William J. Dixon, Jacqueline F. Hamilton, Alastair C. Lewis
Summary: This study presents an automated method for non-targeted compositional analysis of environmental matrices, which has been rigorously tested for reliability and reproducibility using authentic standards. The method detected over 9600 compounds from critical pollutant sources in environmental samples, demonstrating its ability for rapid and comprehensive compound detection.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Energy & Fuels
Jian-Fang Xu, Fei Wang, Xing Fan, Turghun Muhammad, Peter N. Kuznetsov, Ya-Ya Ma
Summary: This study investigated the thermal dissolution process of soft coal and hard coal using different organic solvents. By employing off-line prep-HPLC, the soluble fractions were reliably separated and enriched, leading to the identification of heavy aromatic compounds not detected by GC/MS. The differences in composition between the two coals were elucidated, providing theoretical guidance for efficient coal utilization and mine safety.
Article
Chemistry, Medicinal
Daniela Barcenas-Perez, Antonin Strizek, Pavel Hrouzek, Jiri Kopecky, Marta Barradas, Arantzazu Sierra-Ramirez, Pablo J. Fernandez-Marcos, Jose Cheel
Summary: This study developed a multiple sequential injection HPCCC method to isolate fucoxanthin from Phaeodactylum tricornutum with high purity and recovery. The isolated fucoxanthin retained its biological activity and may provide a useful model for producing fucoxanthin from diatom biomass. Environmental risk and general process evaluation factors were used to assess the developed separation method and compare it with existing methods.
Article
Nutrition & Dietetics
Sarah Brajkovic, Nils Rugen, Carlos Agius, Nicola Berner, Stephan Eckert, Amirhossein Sakhteman, Claus Schwechheimer, Bernhard Kuster
Summary: Plants are crucial for sustainable global food supply, but the understanding of crop proteomes is limited. The presence of secondary metabolites in crop plants has hindered proteomic analyses, requiring individually optimized protein extraction protocols. In this study, a universal protein extraction protocol combined with an automated SP3 protocol and optimized micro-LC-MS/MS conditions was developed for high-quality proteomic analysis of crop plants. The workflow was successfully applied to analyze the proteomes of mature tomato fruits, demonstrating its robustness and potential for large-scale projects mapping crop tissue proteomes.
Article
Agriculture, Multidisciplinary
Sarah Schlag, Soren Goetz, Felix Ruettler, Sandra M. Schmoeckel, Walter Vetter
Summary: This study analyzed phytosterols in 34 different quinoa accessions and identified multiple sterols using gas chromatography with mass spectrometry. A novel internal standard was used for quantification and minor sterols were determined after enrichment. The total sterol content in quinoa was found to be higher than previously reported, with & UDelta;7-sterols contributing the most. Cluster analysis grouped the accessions into four distinct groups based on different sterol patterns.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemical Research Methods
Johannes B. Mueller-Reif, Fynn M. Hansen, Lisa Schweizer, Peter Treit, Philipp E. Geyer, Matthias Mann
Summary: Reversed-phase HPLC is commonly used in MS-based proteomics for peptide separation, with particle-packed capillary columns being predominantly used in nanoflow HPLC systems. Despite capillary columns being a standard for many years, they are expensive and have short lifetimes, leading many laboratories to produce their own in-house packed columns.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Chemistry, Medicinal
Carla Monteiro Leal, Suzana Guimaraes Leitao, Romain Sausset, Simony C. Mendonca, Pedro H. A. Nascimento, Caio Felipe de Araujo R. Cheohen, Maria Eduarda A. Esteves, Manuela Leal da Silva, Tayssa Santos Gondim, Maria Eduarda S. Monteiro, Amanda Resende Tucci, Natalia Fintelman-Rodrigues, Marilda M. Siqueira, Milene Dias Miranda, Fernanda N. Costa, Rosineide C. Simas, Gilda Guimaraes Leitao
Summary: This study isolated three compounds from the leaves of Siparuna cristata and found that two of them showed inhibitory effects against SARS-CoV-2 replication, with a higher selectivity index compared to lopinavir/ritonavir and chloroquine. Flavonoids and their derivatives could be potential candidates for future clinical trials in combating coronavirus infections.
REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY
(2021)
Article
Biochemistry & Molecular Biology
Emilia Marchei, Maria Alias Ferri, Marta Torrens, Magi Farre, Roberta Pacifici, Simona Pichini, Manuela Pellegrini
Summary: The study utilized high-sensitivity GC-MS and UHPLC-HRMS technologies to analyze classic drugs and new psychoactive substances in the urine of heroin addicts under methadone maintenance therapy. Around 80 different psychoactive substances and/or metabolites were identified, with methadone and its metabolites being the most prevalent. This screening for a large number of psychotropic substances could be beneficial in suspected drug-related fatalities or acute intoxications in emergency departments and drug addiction services.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Yuka Terajima, Ryosuke Nagatomo, Mari Nunome, Shuichi Harada, Koichi Inoue
Summary: Isolating valuable chemicals is crucial in reagent manufacturing for pharmaceutical and food science industries. To address sustainability concerns, a sustainable chromatographic purification method using HSCCC was developed, successfully purifying milbemectin while reducing organic solvent consumption.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemistry & Molecular Biology
Magdalena Sliwka-Kaszynska, Iwona Anusiewicz, Piotr Skurski
Summary: The retro-Diels-Alder fragmentation mechanism of luteolin was studied using theoretical calculations and experimental results. The reaction paths and energy barriers leading to the formation of fragment ions were described and discussed.
Article
Chemistry, Analytical
Piia Liigand, Jaanus Liigand, Karl Kaupmees, Anneli Kruve
Summary: This study discusses the variation of ionization efficiency for different compounds and its influencing factors, analyzes the methodology used for measuring ionization efficiency, and the observed data trends. Suggestions are made to collectively collect and unify knowledge about the ionization processes through a GitHub repository.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Xiao-wei Zhang, Yu-ting Chen, Xue Feng, Lu-ya Li, Ke-wei Song, Yu-peng Sun, Guo-hua Zhang, Lan-tong Zhang
Summary: In this study, the metabolism of celastrol in vivo and in vitro was comprehensively characterized using ultra-high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry. A total of 43 metabolites were identified and characterized, and it was found that the biotransformation capacity of the intestinal tract exceeded that of the liver. These findings provide a basis for understanding the pharmacological mechanisms of celastrol and have potential implications for clinical applications.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Biochemical Research Methods
Isin T. Sakallioglu, Amith S. Maroli, Aline De Lima Leite, Robert Powers
Summary: This study introduces a rapid reversed-phase ultra-high-performance liquid chromatography-high resolution mass spectrometry method for mycobacterial lipidomics analysis, which achieves separation of various lipid classes specific to mycobacterium in a short runtime of 14 minutes. The method demonstrates high sensitivity and faster sample throughput compared to existing methods.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Applied
Beatriz Sepulveda, Derin Benites, Laura Albornoz, Mario Simirgiotis, Olivio Castro, Olimpo Garcia-Beltran, Carlos Areche
Summary: This study reports a green extraction method for lichen substances from Hypotrachyna cirrhata using ethyl lactate and high power ultrasounds. The results showed that ethyl lactate can be a sustainable alternative to toxic solvents like methanol, and also demonstrated higher antioxidant activity.
NATURAL PRODUCT RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Javier Palacios, Adrian Paredes, Marcelo A. Catalan, Chukwuemeka R. Nwokocha, Fredi Cifuentes
Summary: Senecio nutans Sch. Bip. and its constituents have antihypertensive effects and cause vasorelaxation by regulating calcium influx and release.
Article
Plant Sciences
Javier Palacios, Adrian Paredes, Fredi Cifuentes, Marcelo A. Catalan, Angel Luis Garcia-Villalon, Jorge Borquez, Mario J. Simirgiotis, Matthew Jones, Amy Foster, David J. Greensmith
Summary: The plant Senecio nutans SCh. Bip. is used by Andean communities to treat altitude sickness. Recent evidence suggests it may produce vasodilation and negative cardiac inotropy, though the cellular mechanisms have not been elucidated.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Alfredo Torres-Benitez, Jose Erick Ortega-Valencia, Marta Sanchez, Mathias Hillmann-Eggers, Maria Pilar Gomez-Serranillos, Gabriel Vargas-Arana, Mario J. Simirgiotis
Summary: This study investigated the metabolomic characterization and biological potential of two lichen species, Cladonia chlorophaea and C. gracilis, in maritime Antarctica. Nineteen compounds were identified in each species, with better results in C. chlorophaea. The study demonstrates the potential of Cladonia species in the prevention and treatment of central nervous system pathologies, inflammatory disorders, and metabolic alterations.
Article
Food Science & Technology
Lucia Cuesta Ramos, Javier Palacios, Ruth E. Barrientos, Jessica Gomez, Juan Manuel Castagnini, Francisco J. Barba, Alejandro Tapia, Adrian Paredes, Fredi Cifuentes, Mario J. Simirgiotis
Summary: This study investigated the proximal analysis, phenolic fingerprinting, antioxidant activity, and enzymatic inhibition and relaxation effects of Azara dentata Ruiz & Pav. in rat aorta. The fruits of this plant exhibited high mineral and heavy metal contents, as well as significant amounts of anthocyanins, flavonoids, phenolic acids, and coumarins. The ethanolic extracts showed strong antioxidant activity and inhibited important enzymes, while also inducing relaxation in rat aorta through the release of endothelial nitric oxide.
Article
Multidisciplinary Sciences
Wohler Gonzales Saenz, Luz Marina Acharte Lume, Javier Camilo Poma Palacios, Victor Guillermo Sanchez Araujo, Filimon Alejandro Quispe Coica, Roc Meseguer Pallares
Summary: Monitoring the quality of water in the high Andean rural area of Peru is vital for ensuring its availability, accessibility, and freedom from fecal and chemical contamination, aligned with sustainable development goals. This study aimed to provide updated information on the status of water quality in six high Andean rural communities in Huancavelica, Peru. The results showed that while most physicochemical parameters were within the allowed threshold, microbiological parameters exceeded the limit value for human consumption in 23.5% of the monitoring points. Additionally, a correlation was found among these microbiological parameters.
REVISTA INVESTIGACIONES ALTOANDINAS-JOURNAL OF HIGH ANDEAN RESEARCH
(2023)
Review
Integrative & Complementary Medicine
Gabriel Vargas-Arana, Elsa Rengifo-Salgado, Mario J. Simirgiotis
Summary: Diabetes mellitus is a metabolic disorder characterized by chronic hyperglycemia and altered metabolism of carbohydrates, proteins, and lipids. It is the leading cause of death in developed countries. This review focuses on medicinal plants from the Peruvian Amazon used in diabetes treatment and their hypoglycemic properties reported in the literature over the past two decades. Out of 77 reported plants, 46.75% show hypoglycemic activity, indicating the potential of traditional knowledge as a source for antidiabetic drugs and future research.
BOLETIN LATINOAMERICANO Y DEL CARIBE DE PLANTAS MEDICINALES Y AROMATICAS
(2023)
Article
Cell Biology
Gabriela Yuri, Sofia Sanhueza, Adrian Paredes, Glauco Morales, Mariana Cifuentes, Paulina Ormazabal
Summary: Elevated fatty acid levels in obesity can cause liver inflammation, fibrosis, and NAFLD. Impaired communication between hepatocytes and adipose tissue leads to adipose lipolysis and fibrosis, negatively impacting metabolic function. The plant extract HEL can counteract fibrotic marker expression and lipolysis caused by obesity, suggesting a preventive approach against hepatic fibrosis and liver-adipose crosstalk.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2023)
Article
Food Science & Technology
Ruth E. Barrientos, Javier Romero-Parra, Fredi Cifuentes, Javier Palacios, Nestor Jaime Romero-Jola, Adrian Paredes, Gabriel Vargas-Arana, Mario J. Simirgiotis
Summary: In this study, the chemical and biological analysis of canelo fruits from Chile was reported for the first time. The fruits were found to be rich in secondary metabolites and could inhibit enzymes associated with neurodegenerative diseases, potentially inducing a hypotensive effect in rat aorta.
Article
Chemistry, Medicinal
Maximiliano Martinez-Cifuentes, Emmanuel Soto-Tapia, Camila Linares-Pipon, Ben Bradshaw, Paulina Valenzuela-Hormazabal, David Ramirez, Patricio Munoz-Torres, Claudio Parra
Summary: This study proposes the design of beta-keto esters as antibacterial compounds. By synthesizing eight beta-keto ester analogues and conducting computational analysis, compound 8 was found to have the most promising potential. In vitro antimicrobial screening showed that compounds 6 and 8 exhibited the most promising activity against the panel of bacteria studied.
Meeting Abstract
Cardiac & Cardiovascular Systems
Bernardita Gonzalez, Pilar Munoz, Claudio Parra, Catherine Bravo
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
(2023)
