Article
Chemistry, Applied
Nemanja Mijin, Jelica Milosevic, Sanja Stevanovic, Predrag Petrovic, Aleksandar Lolic, Tomaz Urbic, Natalija Polovic
Summary: The aggregation of proteins into fibrillar, amyloid-like aggregates has positive effects on various technological properties of food products. Heavy metal ions, such as lead and cadmium, can influence this aggregation process and alter the morphology of the aggregates. This study investigated the impact of lead and cadmium ions on the aggregation of ovalbumin, a model protein, under high temperature and acidic conditions. The results demonstrated that the binding of these metal ions to ovalbumin affected the structure and morphology of the resulting amyloid-like aggregates.
FOOD HYDROCOLLOIDS
(2023)
Article
Materials Science, Multidisciplinary
Valentina Rohnacher, Florian Ullrich, Helge Eggers, Fabian Schackmar, Sebastian Hell, Adriana Salazar, Christian Huck, Gerardo Hernandez-Sosa, Ulrich W. Paetzold, Wolfram Jaegermann, Annemarie Pucci
Summary: Solution-processed tin oxide (SnOx) electron transport layers demonstrate excellent performance in various optoelectronic devices and offer the ease of deposition by various printing techniques. The properties of SnOx thin films have been investigated using various spectroscopies and microscopy techniques, showing improvements in structural and chemical properties with increasing annealing temperature. High performance perovskite solar cells with stabilized power conversion efficiency over 15% demonstrate the great potential of printed SnOx layers in solar energy applications.
ADVANCED MATERIALS TECHNOLOGIES
(2021)
Article
Chemistry, Analytical
Fei-Li Li, Jun Xie, Song Wang, Yang Wang, Chang-Hua Xu
Summary: A new methodology combining MM-IR and 2T-2DCOS has been developed for qualitative and quantitative determination of DON in wheat flour. The method successfully classified wheat flour samples with different concentrations of DON using PCA and SVM, demonstrating high accuracy rates.
Article
Chemistry, Analytical
Oumayma Boukria, El Mestafa El Hadrami, Shakhnoza Sultanova, Randah Miqbil Alqurashi, Yana Cahyana, Abderrahmane Ait-Kaddour
Summary: The aim of this study was to evaluate the potential of mid-infrared spectroscopy combined with two-dimensional correlation spectroscopy (2DCOS) and partial least squares discriminant analysis (PLS-DA) in monitoring the molecular structure evolution of milk mixtures during enzymatic coagulation. Different milk formulations were investigated using MIR spectroscopy, and the synchronous 2DCOS spectra and their auto-peaks revealed formulation dissimilarities and the sequence of molecular structure modifications during coagulation. PLS-DA analysis of the 2DCOS-MIR maps showed high discriminant accuracy between each formulation. Texture characteristics of the resulting gels confirmed the consistency between 2DCOS MIR observations and textural observations.
Article
Environmental Sciences
Hongwei Luo, Qianqian Cheng, Dongqin He, Ganning Zeng, Jianqiang Sun, Jun Li, Xiangliang Pan
Summary: This study investigated the influence of solar radiation and sulfide addition reaction on the complexation of methylmercury (MeHg) with dissolved organic matter (DOM). Fluorescence and FT-IR spectroscopic analysis showed that different treatments resulted in changes in the affinity of binding sites in DOM for MeHg.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Analytical
Thulya Chakkumpulakkal Puthan Veettil, Diana Alves, Jitraporn Vongsvivut, Rosemary L. Sparrow, Bayden R. Wood, Gil Garnier
Summary: Oxidative treatment of RBCs prior to freeze-drying stabilizes the cells for storage at room temperature. Synchrotron-based FTIR microspectroscopy was used to analyze single RBCs and compare lipid and protein spectral data before and after oxidation and freeze-drying. The analysis showed that oxidized and freeze-dried RBCs have different spectral profiles compared to untreated RBCs, indicating changes in lipid peroxidation, stiffness of the membrane, and protein secondary structure. Freeze-drying did not introduce additional changes. This study highlights the potential of freeze-dried RBCs as a stable source for blood serology testing and demonstrates the power of synchrotron FTIR microspectroscopic live-cell analysis.
Article
Biochemistry & Molecular Biology
Sivakumaran Karthikeyan, Monica Maribel Mata-Miranda, Adriana Martinez-Cuazitl, Raul Jacobo Delgado-Macuil, Francisco Garibay-Gonzalez, Virginia Sanchez-Monroy, Alberto Lopez-Reyes, Marlon Rojas-Lopez, Daniel Enrique Rivera-Alatorre, Gustavo Jesus Vazquez-Zapien
Summary: This study compared the immune response in saliva associated with COVID-19 using two-dimensional correlation spectroscopy (2DCOS) and receiver operating characteristic (ROC) analysis, which has potential for non-invasive monitoring of the disease. The results showed biomolecular changes related to SARS-CoV-2 and demonstrated high sensitivity and specificity, confirming the effectiveness of this method in COVID-19 diagnosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Article
Spectroscopy
Daniel Cozzolino, Anh Dao T. Phan, Michael Netzel, Heather Smyth, Yasmina Sultanbawa
Summary: The effects of drying and the addition of maltodextrin on Kakadu plum puree powder samples were evaluated using 2DCOS and NIR spectroscopy, providing insights into developing an effective and easy protocol for determining the drying method.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Zhongyue Guo, Yeran Bai, Meng Zhang, Lu Lan, Ji-Xin Cheng
Summary: Antimicrobial resistance poses a major threat to global health and economics, necessitating rapid AST methods. We introduce a rapid AST method based on wide-field mid-infrared photothermal imaging, capable of metabolic imaging for hundreds of bacteria at single-cell resolution within seconds. This method enables probing of perturbed microbial protein synthesis within 1 hour after antibiotic treatment in E. coli cells, providing insights into antibiotic susceptibility.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yi Qi, Ruochong Zhang, Poongkulali Rajarahm, Shuyan Zhang, Amalina Binte Ebrahim Attia, Renzhe Bi, Malini Olivo
Summary: In this study, a portable confocal Raman spectroscopy (CRS) system with a simultaneous dual-wavelength source and a miniaturized handheld probe was developed. A novel algorithm was also developed for accurate spectral separation. The results demonstrated that the proposed CRS system and algorithm greatly improve the integration of CRS in the clinical workflow.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yi Qi, Ruochong Zhang, Poongkulali Rajarahm, Shuyan Zhang, Amalina Binte Ebrahim Attia, Renzhe Bi, Malini Olivo
Summary: In this study, a portable confocal Raman spectroscopy system with a simultaneous dual-wavelength source and a miniaturized handheld probe was developed. The system can acquire spectra in both fingerprint and high wavenumber regions simultaneously. An innovative design and a unique Raman spectra separation algorithm were implemented to improve the efficiency and accuracy of spectra acquisition.
ANALYTICAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Olga Makshakova, Liliya Bogdanova, Dzhigangir Faizullin, Diliara Khaibrakhmanova, Sufia Ziganshina, Elena Ermakova, Yuriy Zuev, Igor Sedov
Summary: This study demonstrates that certain linear anionic polysaccharides have the ability to disaggregate amyloid fibrils and promote protein renaturation. These findings contribute to our understanding of the mechanism of amyloid disaggregation and provide a foundation for the development of new therapeutic agents for amyloidosis treatment and the design of novel functional protein-polysaccharide complex-based nanomaterials.
Article
Chemistry, Multidisciplinary
Andrea Konecna, Fadil Iyikanat, F. Javier Garcia de Abajo
Summary: The study demonstrates the possibility of identifying single isotope atom impurities in a nanostructure using transmission electron microscopy and spectroscopy. It shows the superior spatial resolution and ability to probe complete sets of vibrational modes by electron beams compared to conventional far-field spectroscopy.
Article
Physics, Applied
Nathan R. Gemmell, Jefferson Florez, Emma Pearce, Olaf Czerwinski, Chris C. Phillips, Rupert F. Oulton, Alex S. Clark
Summary: Sensing with undetected photons allows measurement of absorption and phase shifts at different wavelengths. Experimental mapping in a nondegenerate nonlinear interferometer shows sensitivity recovery despite internal losses at the detection wavelength. An ultrasensitive infrared imaging methodology capable of probing samples effectively in the dark is demonstrated.
PHYSICAL REVIEW APPLIED
(2023)
Article
Biochemistry & Molecular Biology
Kiryl Zhaliazka, Dmitry Kurouski
Summary: In this study, AFM-IR spectroscopy was used to investigate the secondary structure changes of A β peptide during aggregation. It was found that at the early stage, parallel β-sheet oligomers dominated, while antiparallel β-sheet oligomers appeared in the middle stage and coexisted with the parallel β-sheet fibrils at the late stage.
ACS CHEMICAL NEUROSCIENCE
(2022)
Article
Instruments & Instrumentation
Luca Quaroni
INFRARED PHYSICS & TECHNOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Mary-Ambre Carvalho, Herve Dekkiche, Sebastien Richeter, Corinne Bailly, Lydia Karmazin, Declan McKearney, Daniel B. Leznoff, Guillaume Rogez, Bertrand Vileno, Sylvie Choua, Romain Ruppert
JOURNAL OF PORPHYRINS AND PHTHALOCYANINES
(2020)
Article
Chemistry, Multidisciplinary
Enrico Falcone, Ikhlas M. M. Ahmed, Valentina Oliveri, Francesco Bellia, Bertrand Vileno, Youssef El Khoury, Petra Hellwig, Peter Faller, Graziella Vecchio
CHEMISTRY-A EUROPEAN JOURNAL
(2020)
Article
Chemistry, Medicinal
Salen Kuresepi, Bertrand Vileno, Jean-Pierre Lepoittevin, Elena Gimenez-Arnau
CHEMICAL RESEARCH IN TOXICOLOGY
(2020)
Article
Nanoscience & Nanotechnology
Marvin Motay, David Martel, Bertrand Vileno, Charline Soraru, Lydie Ploux, Maria Guadalupe Mendez-Medrano, Christophe Colbeau-Justin, Gero Decher, Nicolas Keller
ACS APPLIED MATERIALS & INTERFACES
(2020)
Correction
Chemistry, Analytical
Adrian Cernescu, Michal Szuwarzynski, Urszula Kwolek, Pawel Wydro, Mariusz Kepczynski, Szczepan Zapotoczny, Maria Nowakowska, Luca Quaroni
ANALYTICAL CHEMISTRY
(2021)
Article
Dermatology
J. Lichter, M. Silva e Sousa, N. Peter, F. Sahli, B. Vileno, S. Kuresepi, C. Gourlaouen, E. Gimenez-Arnau, B. Bloemeke
Summary: Skin sensitization to hydroperoxides from commonly used fragrances can lead to allergic reactions, possibly through radical intermediates. This study found clear individual differences in DC maturation induced by different hydroperoxides, suggesting small structural alterations translate into specific immune responses. Understanding the differing activation levels and frequencies of DCs by these compounds may provide insights into sensitization mechanisms.
BRITISH JOURNAL OF DERMATOLOGY
(2021)
Correction
Chemistry, Analytical
Luca Quaroni
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Tommaso Vannocci, Luca Quaroni, Antonio de Riso, Giulia Milordini, Magda Wolna, Gianfelice Cinque, Annalisa Pastore
Summary: In this study, infrared microscopy was used to monitor the metabolic turnover of individual mammalian cells in real time, revealing major biochemical differences between adherent cells and suspended cells. Spectroscopic variations and spectral oscillations in the infrared domain were observed when using synchrotron infrared radiation, suggesting a potential application as a reporter of cellular conditions. These findings establish IR microscopy as a valuable label-free method for studying real-time metabolism in cells with distinct morphological states.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Long Lin, Xiaogang Peng, Emilie Voirin, Bertrand Donnio, Mircea V. V. Rastei, Bertrand Vileno, Jean-Louis Gallani
Summary: By comparing the behavior of single-crystalline and multi-twinned silver nanoparticles, the influence of core crystallinity on magnetic properties was investigated. The results show that single-crystalline nanoparticles exhibit classical paramagnetic behavior, while multi-twinned nanoparticles show a combination of para- and ferromagnetic behaviors. Our hypothesis is that lattice defects within the core carry magnetic moments which couple through conduction electrons, with dipolar interactions also playing a local and macroscopic role.
HELVETICA CHIMICA ACTA
(2023)
Article
Nanoscience & Nanotechnology
Ludivine Larue, Tataye Moussounda Moussounda Koumba, Nolwenn Le Breton, Bertrand Vileno, Philippe Arnoux, Valerie Jouan-Hureaux, Cedric Boura, Gerard Audran, Raphael Bikanga, Sylvain R. A. Marque, Samir Acherar, Celine Frochot
Summary: Photodynamic therapy (PDT) is a promising technique for treating diseases like cancer, but currently faces challenges with PS selectivity and oxygen dependence. Targeting tumor neovessels and using Alk to compensate for these issues, a multifunctional compound was successfully synthesized, providing new possibilities for the advancement of PDT.
ACS APPLIED BIO MATERIALS
(2021)
Article
Chemistry, Analytical
Luca Quaroni
Article
Chemistry, Analytical
Kirk H. H. Michaelian, Mark D. D. Frogley, Gianfelice Cinque, Luca Quaroni