Rapid detection model of Bacillus subtilis in solid-state fermentation of rapeseed meal

The main objective of this research was to monitor variety in the content of Bacillus subtilis 10160 during solid-state fermentation (SSF) of rapeseed meal by near infrared (NIR) spectroscopy and chemometrics. Observations showed the coefficient of determination (R-2) value was 0.9401 and root-mean-square deviation was 0.639 log (CFU/g) for viable cell content by synergies between four intervals 5,203.003-5,600.267, 5,604.124-6,001.388, 6,807.487-7,204.751 and 8,411.972-8,809.235 cm(-1). The determination coefficient of prediction (R-p(2)) and root-mean-square deviation of prediction in an external validation set could reach 0.9532 and 0.628 log (CFU/g) by the viable B. subtilis model. These findings suggest that rapid detection of B. subtilis in SSF is achieved by the combination of synergy interval partial least squares and NIR spectroscopy.