Article
Agronomy
Xuanjun Feng, Yue Hu, Weixiao Zhang, Rongqian Xie, Huarui Guan, Hao Xiong, Li Jia, Xuemei Zhang, Hanmei Zhou, Dan Zheng, Ying Wen, Qingjun Wang, Fengkai Wu, Jie Xu, Yanli Lu
Summary: The P5CS gene plays a protective role in stressed plants, but overexpression of P5CS may inhibit plant growth. By expressing AtP5CS1 and AtP5CS1F128A genes under the regulation of an ABA-inducible promoter, it was found that seedling growth and grain yield of rice can be increased, but it has detrimental effects on grain quality, tiller number, and grain yield.
Review
Plant Sciences
U. K. Ghosh, M. N. Islam, M. N. Siddiqui, X. Cao, M. A. R. Khan
Summary: Proline plays a crucial role in plant response to abiotic stresses, acting as a molecular chaperone, antioxidative defence molecule, osmoprotectant, and signaling molecule. Overproduction of proline in plants helps maintain cellular homeostasis and improve adaptive responses to changing climate conditions.
Article
Biochemistry & Molecular Biology
Farman Ali, Qi Wang, Aliya Fazal, Lin-Juan Wang, Shuyan Song, Meng-Juan Kong, Tariq Mahmood, Shan Lu
Summary: In this study, we found that the expression of OR, a DnaJ-like zinc finger protein, was upregulated in response to drought treatment in Arabidopsis thaliana. The overexpression of OR in seedlings resulted in improved growth performance and survival rate under drought stress. Further investigation revealed that the enhanced drought tolerance in OR-overexpressing seedlings was associated with lower reactive oxygen species levels, higher antioxidant enzyme activities, and increased proline content. Our findings also demonstrated that the activity of the rate-limiting enzyme for proline biosynthesis, P5CS, could be enhanced by exogenous OR supplementation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Yao Zheng, Cecile Cabassa-Hourton, Holger Eubel, Guillaume Chevreux, Laurent Lignieres, Emilie Crilat, Hans-Peter Braun, Sandrine Lebreton, Arnould Savoure
Summary: This study reveals evidence of physical proximity and interactions among ProDH, P5CDH, and OAT in the mitochondria of Arabidopsis during dark-induced leaf senescence, suggesting the formation of a complex during P5C metabolism.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Panaiotis M. Stassinos, Massimiliano Rossi, Ilaria Borromeo, Concetta Capo, Simone Beninati, Cinzia Forni
Summary: Seed priming with spermidine was found to significantly improve plant tolerance to salt stress by enhancing photosynthetic pigments, proline accumulation, and antioxidant responses, regardless of genotype. This approach may offer new perspectives for enhancing crop productivity in saline soils.
Article
Plant Sciences
Vanesa S. Garcia de la Torre, Teodoro Coba de la Pena, M. Mercedes Lucas, Jose J. Pueyo
Summary: This study found that transgenic plants displayed better growth performance and higher Cd tolerance under Cd stress.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Tiantian Liu, Yuxin Li, Chang Wang, Da Zhang, Jiajia Liu, Mingyuan He, Mingxun Chen, Yuan Guo
Summary: This study revealed the function of Brassica napus WRKY transcription factors in leaf senescence, providing a novel strategy for breeding new stay-green cultivars in rapeseed through genetic manipulation.
Review
Biochemistry & Molecular Biology
Alexandra N. Bogner, Kyle M. Stiers, John J. Tanner
Summary: Proline metabolism plays a significant role in cancer cells, involving upregulation of proline biosynthetic genes and the dual function of proline catabolic enzyme in promoting cancer and apoptosis. The cycling of proline impacts cellular growth and death pathways. PYCR, the last enzyme in proline biosynthesis, is crucial for the proline metabolic cycle and has been shown to be widely upregulated in various cancer types.
Article
Plant Sciences
Yao Zheng, Cecile Cabassa-Hourton, Severine Planchais, Emilie Crilat, Gilles Clement, Matthieu Dacher, Nina Durand, Marianne Bordenave-Jacquemin, Anne Guivarc'h, Corentin Dourmap, Pierre Carol, Sandrine Lebreton, Arnould Savoure
Summary: During leaf senescence, nitrogen is remobilized and carbon backbones are replenished through amino acid catabolism in mitochondria. Metabolic changes were studied in Arabidopsis thaliana leaves of different mutants, and it was found that the levels of P5C and proline were higher in p5cdh mutant than in prodh1prodh2 mutant. Furthermore, the down-regulation of ProDH1 activity and protein amount in the p5cdh mutant played a critical role in determining the flow through amino acid remobilization pathways.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Plant Sciences
Long Wang, Jingdong Zheng, Guisheng Zhou, Jing Li, Chen Qian, Guobin Lin, Yiyang Li, Qingsong Zuo
Summary: Salt stress limits plant growth, but nitrogen application is an effective strategy to alleviate its negative effects.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Wei Liu, Zhonghua Wang, Rui Ren, Yan Liu, Lunwen Qian, Mei Guan, Chunyun Guan, Xin He
Summary: This study investigated the molecular mechanisms of salt and osmotic stress response in rapeseed. The researchers identified differentially expressed genes and early regulated genes, and found that overexpression of a specific transcription factor (BnaFBH3-C06) improved salt tolerance and early flowering in rapeseed. These findings provide insights into the response to abiotic stresses in rapeseed and offer a potential candidate gene for molecular breeding of salt-tolerant and early-maturing rapeseed cultivars.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Muhammad Ahmad, Ejaz Ahmad Waraich, Saddam Hussain, Choudhary Muhammad Ayyub, Zahoor Ahmad, Usman Zulfiqar
Summary: The study found that thiourea seed priming improved the growth and yield of camelina and canola under heat stress conditions by maintaining gas-exchange attributes, water relations, and upregulating antioxidants. Cultivars 618 and 45S42 showed better antioxidant activity to maintain redox potential in camelina and canola, respectively. This improvement in heat stress tolerance is attributed to higher accumulation of secondary metabolites, better antioxidative defense, and maintenance of plant water status in thiourea-primed seedlings.
JOURNAL OF PLANT GROWTH REGULATION
(2022)
Article
Agronomy
Martina Komarkova, Zuzana Kovalikova, Jiri Simek, Adam Skarka, Jiri Tuma
Summary: Different rapeseed cultivars showed varying physiological responses and accumulation abilities to high doses of cadmium. Cadmium was found to accumulate more in roots than in shoots. CZL and Benefit had higher translocation factors at the lowest dose of cadmium. The content of potassium and calcium varied between treatments and cultivars. Minimal effects were observed on chlorophyll fluorescence parameters and relative chlorophyll content. High levels of salicylic acid and proline content were mainly found in Iwas, indicating its higher tolerance to cadmium.
PLANT SOIL AND ENVIRONMENT
(2022)
Article
Plant Sciences
Felix Frimpong, Carel W. Windt, Dagmar van Dusschoten, Ali A. Naz, Michael Frei, Fabio Fiorani
Summary: The study found that barley genotypes carrying a wild allele at P5cs1 locus have an advantage in drought resistance at the reproductive stage by accumulating proline, maintaining photosynthetic activity, and intrinsic water use efficiency, ultimately achieving higher seed productivity.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agriculture, Multidisciplinary
Shuangcheng He, Rong Ma, Zijin Liu, Da Zhang, Shixiang Wang, Yuan Guo, Mingxun Chen
Summary: This study reveals that overexpression of BnaAGL11 in Brassica napus results in smaller, curly leaves and accelerated leaf senescence. This is achieved through the regulation of multiple gene transcription, where BnaAGL11 indirectly controls leaf morphogenesis and senescence.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Microbiology
Younes Dellero, Suzanne Rose, Coralie Metton, Christian Morabito, Josselin Lupette, Juliette Jouhet, Eric Marechal, Fabrice Rebeille, Alberto Amato
ENVIRONMENTAL MICROBIOLOGY
(2018)
Review
Biochemistry & Molecular Biology
Christian Morabito, Caroline Bournaud, Cecile Maes, Martin Schuler, Riccardo Aiese Cigliano, Younes Dellero, Eric Marechal, Alberto Amato, Fabrice Rebeille
PROGRESS IN LIPID RESEARCH
(2019)
Article
Microbiology
Younes Dellero, Cecile Maes, Christian Morabito, Martin Schuler, Caroline Bournaud, Riccardo Aiese Cigliano, Eric Marechal, Alberto Amato, Fabrice Rebeille
ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Younes Dellero, Maud Heuillet, Nathalie Marnet, Floriant Bellvert, Pierre Millard, Alain Bouchereau
Review
Plant Sciences
Younes Dellero
FRONTIERS IN PLANT SCIENCE
(2020)
Article
Plant Sciences
Younes Dellero, Mathieu Jossier, Alain Bouchereau, Michael Hodges, Laurent Leport
Summary: Leaf senescence can lead to the degradation of chloroplast components, affecting photosynthetic properties of plants; Different oilseed rape leaf phenological stages have distinct characteristics; There are contrasting water status regulations between leaves of different stages in winter oilseed rape.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Younes Dellero, Caroline Mauve, Mathieu Jossier, Michael Hodges
Summary: Photorespiration is a crucial metabolic process for plant growth that removes toxic substances produced by enzyme activity. Research on Arabidopsis thaliana found that under low atmospheric CO2 concentrations, there were significant changes in leaf amino acid metabolism, particularly involving amino acids such as Gly and Ser. The study highlights the importance of photorespiration in regulating amino acid levels under stress conditions.
Article
Cell Biology
Etienne Deragon, Martin Schuler, Riccardo Aiese Cigliano, Younes Dellero, Gregory Si Larbi, Denis Falconet, Juliette Jouhet, Eric Marechal, Morgane Michaud, Alberto Amato, Fabrice Rebeille
Summary: Thraustochytrids are marine protists that naturally accumulate triacylglycerol with long chains of polyunsaturated fatty acids, such as DHA. Through genome sequencing and metabolic analysis, a peroxisomal adenylate transporter gene (AlANT1) was found to be associated with DHA accumulation in Thraustochytrids, providing a new approach to enhance DHA production in these organisms.
Article
Multidisciplinary Sciences
Gregoire Bianchetti, Vanessa Clouet, Fabrice Legeai, Cecile Baron, Kevin Gazengel, Aurelien Carrillo, Maria J. Manzanares-Dauleux, Julia Buitink, Nathalie Nesi
Summary: Oilseed rape is the third largest oil crop globally, facing unfavorable environmental conditions such as drought and clubroot disease which threaten its yield and seed quality. Combinations of different stresses lead to complex plant responses, and transcriptome datasets are essential for understanding environmental stress-resistance mechanisms in plant organs.
Article
Biochemistry & Molecular Biology
Alban Launay, Sylvie Jolivet, Gilles Clement, Marco Zarattini, Younes Dellero, Rozenn Le Hir, Mathieu Jossier, Michael Hodges, Dominique Expert, Mathilde Fagard
Summary: This study identified the mechanism of resistance to the toxic effects of DspA/E through suppressor mutants and highlighted the importance of glycolate oxidase in type II non-host resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Youssef Bouargalne, Celine Raguenes-Nicol, Florian Guilbaud, Angelique Cheron, Vanessa Clouet, Carole Deleu, Francoise Le Caherec
Summary: This study presents a novel method for assessing chlorophyll binding to water-soluble chlorophyll-proteins (WSCPs) and investigates the binding characteristics and protective effects of a WSCP protein BnD22. The results indicate that BnD22 has high affinity binding to chlorophylls and strong selectivity for Chla.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Plant Sciences
Younes Dellero, Solenne Berardocco, Cecilia Berges, Olivier Filangi, Alain Bouchereau
Summary: The estimation of metabolic fluxes in photosynthetic organisms is a significant challenge, especially with the development of C-13-Metabolic Flux Analysis. To accurately measure Carbon Isotopologue Distribution and minimize uncertainties for the calculated fluxes, the accuracy of GC-MS at the isotopologue level needs to be evaluated. This study developed a workflow to validate GC-MS measurements of Carbon Isotopologue Distributions in plant metabolites by using standardized extracts and investigated the light/dark regulation of the TCA cycle in Brassica napus leaf discs. The findings showed the cyclic operation of the TCA cycle under both light and dark conditions, with interesting contributions of different pathways and reassimilation of carbon derived from mitochondrial enzymes.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Younes Dellero, Olivier Filangi, Alain Bouchereau
Summary: The reliability of GC/MS measurements and computational estimation of C-13-positional enrichments were evaluated in this study using tailor-made C-13-PT compounds. It was found that certain mass fragments had significant biases, leading to errors in the calculation of C-13-positional enrichments. However, a GC/MS-based C-13-positional approach was successfully validated and applied to investigate key metabolic fluxes in plant primary metabolism.
