Article
Food Science & Technology
Xiaoyu Bao, Jianping Wu
Summary: Ovomucin hydrolysate has been shown to prevent pathogen adhesion to intestinal epithelial cells, and in this study, it was demonstrated to improve intestinal barrier integrity by inhibiting inflammatory responses induced by LPS.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Neurosciences
Ling Deng, Jiyu Zhang, Sha Chen, Yu Wu, Xiaomei Fan, Tianrui Zuo, Qingwen Hu, Lu Jiang, Shaonan Yang, Zhi Dong
Summary: In this study, the roles and potential mechanisms of miR-671-5p on blood-brain barrier (BBB) permeability in ischemic stroke (IS) were investigated. The results showed that miR-671-5p was significantly downregulated in the cerebral cortex of IS mice. Administration of miR-671-5p agomir reduced infarct volume, improved neurological deficits, attenuated cell injury, and reduced BBB permeability. Furthermore, the upregulation of miR-671-5p alleviated tight junction degradation by targeting NF-?B/MMP-9.
MOLECULAR NEUROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Ashley Monaco, Ben Ovryn, Josephine Axis, Kurt Amsler
Summary: The paracellular permeability in epithelial cells involves both a Pore Pathway for small ions and solutes, and a Leak Pathway for larger solutes. While the Pore Pathway is better understood, the proteins and mechanism behind the Leak Pathway remain unresolved and controversial. Further research is needed to clarify the molecular basis and regulation of the Leak Pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Weidong Dou, Hao Xu, Shuo Feng, Tao Liu, Lin Xiao, Yingchao Wu, Shanwen Chen, Yisheng Pan, Xin Wang
Summary: This study investigates the protective effects of betaine on intestinal barrier function in a mouse model of ulcerative colitis and reveals its mechanism of action. The results show that betaine protects the intestinal barrier function of mice with ulcerative colitis by inhibiting the activation of the NF-kappa B-mediated signaling pathway.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Agriculture, Multidisciplinary
Yan Wang, Qianqian Xu, Meijuan Meng, Guangjun Chang, Nana Ma, Xiangzhen Shen
Summary: The present study demonstrates that butyrate can alleviate the inflammatory response and tight junction (TJ) injury in bovine mammary epithelial cells (BMECs) by blocking NF-kappa B activation and decreasing inflammatory cytokine production and myosin light chain kinase (MLCK) expression.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Ruicheng Yang, Jundan Wang, Fen Wang, Huipeng Zhang, Chen Tan, Huanchun Chen, Xiangru Wang
Summary: Despite advances in treatment, bacterial meningitis remains a serious infection that poses a risk to life. The blood-brain barrier plays an important role in the pathogenesis of bacterial meningitis through increased permeability. Recent studies have shown that targeting the factors contributing to enhanced blood-brain barrier permeability is an effective complement to antimicrobial therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Sen Yu, Jie He, Keping Xie
Summary: Tight junction (TJ) is a barrier in epithelial and endothelial cells that maintains paracellular substrate transport and cell polarity. Zonula occludens (ZO) proteins, as cytoplasmic adaptor proteins adjacent to the cell membrane, connect transmembrane TJ proteins and cytoplasmic cytoskeleton to maintain barrier function. Besides their structural role, ZO proteins also play important roles in signal regulation, such as cell proliferation, motility, embryonic development, tissue homeostasis, inflammation, tumorigenesis, and cancer progression. This review focuses on the signal regulating function of ZO proteins in inflammation and tumorigenesis, and discusses the limitations and future challenges in ZO protein research.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Junyu Mu, Yuhui Que, Xu Li, Feier Zhou, Lai Jin, Shengnan Li, Chao Zhu
Summary: There may be a linkage between CRHR1 and S1PR2/S1PR3 in regulating endothelial monolayer permeability under oxidative stress conditions.
VASCULAR PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Soyoun Min, Nam Than, Yong Cheol Shin, Grace Hu, Woojung Shin, Yoko M. Ambrosini, Hyun Jung Kim
Summary: In this study, a pathomimetic Leaky Gut Chip was developed to mimic increased epithelial permeability and intestinal inflammation. The effectiveness of probiotic intervention was evaluated using this chip, and it was found that probiotic bacteria could improve barrier function, reduce the expression of inflammatory markers, and decrease the production of pro-inflammatory cytokines.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Toka N. Mahmoud, Walaa H. El-Maadawy, Zeinab A. Kandil, Heba Khalil, Nabaweya M. El-fiky, Taha Shahat M. A. El Alfy
Summary: This study demonstrated the therapeutic potential of CGE against UC by restoring intestinal mucosal barrier integrity, mitigating oxidative stress, inflammatory cascade, as well as inhibiting NF-kappa B/TLR4 and NLRP3 pathways activation in colonic tissues. Additionally, CGE at a dose of 200 mg/kg was more effective in ameliorating DSS-induced UC compared to SAS at the same dose.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yan Wang, Xuerui Li, Zhengqiang Han, Meijuan Meng, Xiaoli Shi, Lairong Wang, Mengru Chen, Guangjun Chang, Xiangzhen Shen
Summary: This study aimed to investigate the effects and mechanisms of iE-DAP-induced inflammatory response on the integrity of tight junctions in bovine mammary epithelial cells (BMECs). The results showed that iE-DAP induced an inflammatory response and disrupted tight junctions, which was associated with increased expression of inflammatory cytokines and decreased expression of ZO-1 and Occludin. The inhibition of MLCK and NF-κB signaling pathways reversed the TJ disruption.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nutrition & Dietetics
Shunan Ren, Aorigele Chen, Yanping Tian, Zhaoxing Bai, Chunjie Wang
Summary: This study investigated the beneficial effect of Lactobacillus paracasei, isolated from koumiss, on a mouse model of diarrhea induced by Escherichia coli O-8 (E. coli O-8). The results showed that L. paracasei reduced intestinal permeability, induced the expression of mucin 2, and increased the number of goblet cells by upregulating the expression of tight junction (TJ) proteins via the nuclear factor kappa B cells-myosin light-chain kinase signaling pathway.
Article
Cell Biology
Yunfei Li, Yifan Xia, Huixiang Zhu, Eric Luu, Guangyao Huang, Yan Sun, Kevin Sun, Sander Markx, Kam W. Leong, Bin Xu, Bingmei M. Fu
Summary: The integrity of the blood-brain barrier is compromised in patients with 22q11.2 deletion syndrome associated with schizophrenia, leading to increased solute permeability, decreased trans-endothelial electrical resistance, and disruption of tight junction proteins and endothelial glycocalyx.
Article
Genetics & Heredity
Hao Ning, Tianyuan Zhang, Xinxin Zhou, Libo Liu, Chao Shang, Ruiqun Qi, Teng Ma
Summary: This study identifies the crucial role of the NOVA2/PART1/PPP2R3A/p-NF κB-p65 pathway in regulating blood-brain barrier permeability in amyloid-β(1-42)-incubated endothelial cells, suggesting a potential mechanism of lncRNA and protein interaction in the pathogenesis of Alzheimer's disease.
Article
Agriculture, Multidisciplinary
Yaping Li, Xiao Pan, Mingyuan Yin, Cuiping Li, Lirong Han
Summary: The study demonstrated the preventive effect of lycopene in dextran sulfate sodium-induced ulcerative colitis in mice by increasing anti-oxidant levels, reducing inflammation, and upregulating tight junction-related proteins through modulation of the TLR4/TRIF/NF-κB signaling pathway.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Sotirios P. Fortis, Anthimia Batrinou, Hara T. Georgatzakou, Ioannis Tsamesidis, Grigorios Alvanidis, Effie G. Papageorgiou, Kontantinos Stamoulis, Dimitrios Gkiliopoulos, Georgia K. Pouroutzidou, Anna Theocharidou, Eleana Kontonasaki, Anastasios G. Kriebardis
Summary: This study evaluated the compatibility of human blood cells with silica-based mesoporous nanomaterials (MSNs) manufactured using the solgel method, with Ca and Ce as doping elements. The results showed that these nanomaterials had no impact on the viability of lymphocytes and monocytes, but reduced the viability of granulocytes. Additionally, the expression of Pselectin in platelets and the level of internal reactive oxygen species increased when exposed to MSNs. The presence of Ce in the MSNs improved their hemocompatibility to some extent. Further research is needed to understand how MSNs may affect different blood components and design safe and effective MSNs for biomedical applications.
CHEMICO-BIOLOGICAL INTERACTIONS
(2024)
Retraction
Biochemistry & Molecular Biology
Tiechao Jiang, Dongli Jiang, Dong You, Lirong Zhang, Long Liu, Qini Zhao
CHEMICO-BIOLOGICAL INTERACTIONS
(2024)
Article
Biochemistry & Molecular Biology
Yuting Chen, Lin Chen, Shiheng Zhu, Hui Yang, Zhongming Ye, Huanhuan Wang, Haipeng Wu, Yao Wu, Qian Sun, Xiaoshan Liu, Hairong Liang, Huanwen Tang
Summary: This study investigates the impact of exosomal derived miR-1246 from HQ-transformed cells on cell-to-cell communication in recipient TK6 cells. The results show that exosomal miR-1246 targets CCNG2, regulating TK6 cell cycle arrest, highlighting its potential as a biomarker for HQ-induced malignant transformation.
CHEMICO-BIOLOGICAL INTERACTIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuping Yu, Yaming Mu, Kai Wang, Ling Wang, Chunying Wang, Zexin Yang, Yu Liu, Shuxian Li, Meihua Zhang
Summary: Fetal growth restriction (FGR) is a common complication in obstetrics, and its exact cause is unknown. In this study, we constructed 1-NP exposed pregnant mice models and found that 1-NP induced FGR. Additionally, we observed significant ferroptosis in placental trophoblasts from 1-NP exposed mice and human FGR patients. Using in vitro cell models, we demonstrated that 1-NP impaired trophoblast biological function and induced cellular ferroptosis. We also identified the ERK signaling pathway and CYP1B1 as key regulators of 1-NP-induced ferroptosis. This study provides new insights into the aetiology of FGR and the reproductive toxicity of environmental pollutants.
CHEMICO-BIOLOGICAL INTERACTIONS
(2024)
Article
Biochemistry & Molecular Biology
Lei Hou, Yingying Zhao, Shiyu Zhao, Xuexia Zhang, Xia Yao, Jianjun Yang, Ziteng Wang, Shuaibing Liu
Summary: This study systematically characterized the UGTs enzymes involved in the formation of M4 and the inhibitory effects of ciprofol and its metabolite M4 on P450s enzymes. In vitro-in vivo extrapolation and PBPK simulations were performed to predict potential drug-drug interactions caused by ciprofol.
CHEMICO-BIOLOGICAL INTERACTIONS
(2024)
Review
Biochemistry & Molecular Biology
Disheng Liu, Lu Wang, Wuhua Ha, Kan Li, Rong Shen, Degui Wang
Summary: Renal fibrosis is a common outcome of renal injuries, characterized by structural destruction and functional decline of the kidneys. Hypoxia induces the activation of HIF-1 alpha, which regulates cellular metabolism, proliferation, apoptosis, and inflammation, contributing to the development of renal fibrosis. Understanding the regulation and cascade reactions mediated by HIF-1 alpha can provide new insights for studying the mechanism of renal fibrosis.
CHEMICO-BIOLOGICAL INTERACTIONS
(2024)
Article
Biochemistry & Molecular Biology
Zhao-Bo Luo, Liu-Hui Yang, Sheng-Zhong Han, Shuang-Yan Chang, Hongye Liu, Zhi-Yong An, Xiu-Li Zhang, Biao-Hu Quan, Xi-Jun Yin, Jin-Dan Kang
Summary: This study demonstrates that cyclophosphamide (CTX) treatment has detrimental effects on oocytes and embryos, leading to DNA damage, apoptosis, and abnormal histone modification. Supplementation with LBH589 can effectively restore the developmental potential of embryos by increasing histone modification levels and restoring protein expression of NF-kappa B, a key regulator of early embryo development.
CHEMICO-BIOLOGICAL INTERACTIONS
(2024)
Article
Biochemistry & Molecular Biology
Sheng Chen, Hanqing Xu, Yi He, Chen Meng, Yunhui Fan, Yunkun Qu, Yingguang Wang, Wei Zhou, Xiaojian Huang, Hongbo You
Summary: Osteoarthritis is a heterogeneous disease that affects the entire joint. This study found that Carveol can reverse the inflammatory state of macrophages, promote their anti-inflammatory effects, and protect cartilage by activating the NRF2/HO-1/NQO1 pathway and reducing ROS clearance. The results also showed that Carveol can alleviate the pathological changes of osteoarthritis in mice, suggesting its potential therapeutic efficacy.
CHEMICO-BIOLOGICAL INTERACTIONS
(2024)
Article
Biochemistry & Molecular Biology
Liyi Wei, Tingting Wang, Mingcui Luo, Shuai Zhang, Mengxi Lu, Xinli Zhou, Xuelei Cheng, Hui Wang, Dan Xu
Summary: This study found that azithromycin during pregnancy may have toxic effects on fetal hippocampal development, especially in the late pregnancy, high dose, and multi-course situation. The results also suggest that the SOX2/Wnt signaling pathway may be involved in this toxicity.
CHEMICO-BIOLOGICAL INTERACTIONS
(2024)
Review
Biochemistry & Molecular Biology
Di Wu, Faheem Ahmed Khan, Kejia Zhang, Nuruliarizki Shinta Pandupuspitasari, Windu Negara, Kaifeng Guan, Fei Sun, Chunjie Huang
Summary: Retinoic acid (RA) is a signaling molecule derived from vitamin A/retinol, with implications in various aspects of health and disease. It regulates cell functioning through both transcriptional and non-genomic mechanisms, influencing cell-fate determination, neurogenesis, visual function, inflammatory responses, and gametogenesis commitment.
CHEMICO-BIOLOGICAL INTERACTIONS
(2024)
Review
Biochemistry & Molecular Biology
Bilal Murtaza, Lili Wang, Xiaoyu Li, Muhammad Yasir Nawaz, Muhammad Kashif Saleemi, Aisha Khatoon, Xu Yongping
Summary: Mycotoxins in food pose significant concerns for food safety and public health, potentially causing a range of adverse symptoms and cancer development. Deoxynivalenol (DON) is particularly worrisome due to its harm to vital organs. Altered mycotoxins present possible risks to the environment and well-being, necessitating further research into their adverse consequences. Accurately assessing the risk posed by modified mycotoxins remains challenging.
CHEMICO-BIOLOGICAL INTERACTIONS
(2024)
Article
Biochemistry & Molecular Biology
Emine Toraman, Buesra Budak, Cemil Bayram, Selma Sezen, Behzad Mokhtare, Ahmet Hacimueftueoglu
Summary: The study suggests that parthenolide (PTL) may have therapeutic effects in treating testicular toxicity caused by paclitaxel (PTX) through reducing oxidative stress and increasing glutathione levels. PTL also promotes the expression of genes involved in reproduction and sperm production.
CHEMICO-BIOLOGICAL INTERACTIONS
(2024)
Correction
Biochemistry & Molecular Biology
Cuicui Zhuang, Hui Huo, Wanfa Fu, Wanyue Huang, Lulu Han, Miao Song, Yanfei Li
CHEMICO-BIOLOGICAL INTERACTIONS
(2024)
Article
Biochemistry & Molecular Biology
Taotao Zhao, Jia Lv, Mingyuan Peng, Jiahui Mi, Shaosan Zhang, Jie Liu, Tong Chen, Zilong Sun, Ruiyan Niu
Summary: This study explores the protective effects of fecal microbiota transplantation (FMT) and short-chain fatty acids (SCFAs) supplementation on learning and memory impairment caused by fluoride exposure in mice. The results show that FMT and SCFAs can improve memory deficits and alleviate pathological damages caused by fluoride, possibly by activating the BDNF-PI3K/AKT pathway. Furthermore, the disordered gut microbiome caused by fluoride can be restored through frequent FMT.
CHEMICO-BIOLOGICAL INTERACTIONS
(2024)
Article
Biochemistry & Molecular Biology
Yong Liu, Zhaofei Pang, Yadong Wang, Jichang Liu, Guanghui Wang, Jiajun Du
Summary: This study reveals that silencing PKD2 promotes ferroptosis in LUAD by increasing reactive oxygen species, malondialdehyde accumulation, intracellular iron content and cell death. Overexpression of PKD2 prevents autophagic degradation of ferritin and promotes proliferation, migration and invasion of LUAD cells. Moreover, targeting PKD2 enhances the efficacy of carboplatin through ferroptosis and apoptosis in LUAD.
CHEMICO-BIOLOGICAL INTERACTIONS
(2024)