Article
Chemistry, Multidisciplinary
Rofice Dickson, Enrico Mancini, Nipun Garg, John M. Woodley, Krist V. Gernaey, Manuel Pinelo, Jay Liu, Seyed Soheil Mansouri
Summary: The study evaluates the economics, environmental impact, risk assessment, and optimal processing route of bio-SA production from different feedstocks, identifying glycerol as the best feedstock and corn stover as the second best. Seaweed is found to be less suitable for large-scale bio-SA production due to high cost and difficulties in enzyme hydrolysis. Environmental results show that the pathway from glycerol is the most environmentally friendly, followed by corn stover, glucose, and seaweed.
ENERGY & ENVIRONMENTAL SCIENCE
(2021)
Article
Agricultural Engineering
Veronica Sayury Nishida, Adenise Lorenci Woiciechowski, Kim Kley Valladares-Diestra, Luis Alberto Zevallos Torres, Luciana Porto de Souza Vandenberghe, Arion Zandona Filho, Carlos Ricardo Soccol
Summary: In this study, soybean hull was pretreated using imidazole as a solvent to produce bioethanol and succinic acid, demonstrating a more environmentally friendly and sustainable production process.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biotechnology & Applied Microbiology
Agata Olszewska-Widdrat, Charilaos Xiros, Anders Wallenius, Roland Schneider, Lais Portugal Rios da Costa Pereira, Joachim Venus
Summary: This work demonstrates the microbial production of lactic acid and succinic acid, two platform chemicals, from a non-expensive side stream of pulp and paper industry, proposing a sustainable way to valorize it towards economically important monomers for bioplastics formation. The highest yield and productivities for lactic and succinic acid production were achieved at 0.99 g.g(-1) and 3.75 g.L-1.h(-1), and 0.77 g.g(-1) and 1.16 g.L-1.h(-1), respectively.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Maria Hijosa-Valsero, Ana Paniagua-Garcia, Rebeca Diez-Antolinez
Summary: The feasibility of using vine shoots and grape must as feedstocks for succinic acid production was evaluated. The results showed that Actinobacillus succinogenes performed better in grape must fermentation, while Basfia succiniproducens had higher demand for yeast extract. In addition, vine shoots released higher concentrations of total sugars after hydrolysis, which favored succinic acid production.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Physical
Waldo Gideon Lexow, Sekgetho Charles Mokwatlo, Hendrik Gideon Brink, Willie Nicol
Summary: A. succinogenes is known for utilizing different catabolic pathways and adjusting its metabolism according to external environment. The microbe optimizes its energy extraction pathways and exhibits an energy-efficient survival strategy. The organism may utilize mechanisms such as pyruvate overflow to regulate the production of acetic and formic acid, showing sensitivity to external concentrations.
Article
Agricultural Engineering
Jun Zhang, Kuntai Li, Shucheng Liu, Shushi Huang, Chao Xu
Summary: The study optimized the AHP pretreatment conditions for sugarcane bagasse, followed by enzymatic hydrolysis at high-solids substrate loading and fed-batch fermentation to convert fermentable sugars into succinic acid (SA), achieving a high SA conversion rate of 0.29 g/g SCB raw material.
BIORESOURCE TECHNOLOGY
(2022)
Article
Engineering, Chemical
Hsiang-Yen Su, Wei-Hai Lin, Ying-Lin Liang, Hsiang-Hui Chou, Si-Wei Wu, Hui-Ling Shi, Jia-Yu Chen, Ke-Ke Cheng
Summary: By genetically engineering the Enterobacter cloacae genome, the CL82 strain was constructed for the co-production of succinic acid and acetoin. CL82 showed high concentrations and productivities of succinic acid and acetoin from glucose, and could also utilize xylose and corncob hydrolysate for co-production. This engineering strategy provides a practical approach for simultaneous production of two commercial products using renewable feedstocks, reducing the cost of microbial fermentation.
CHEMICAL ENGINEERING SCIENCE
(2022)
Article
Multidisciplinary Sciences
Nuraishah Abd Rahim, Abdullah Amru Indera Luthfi, Nurul Adela Bukhari, Jian Ping Tan, Peer Mohamed Abdul, Shareena Fairuz Abdul Manaf
Summary: The aim of this study was to establish an improved pretreatment and fermentation method, immobilized cells, for high recovery of fermentable sugars from palm kernel cake (PKC), and its effects on fermentability performance by Actinobacillus succinogenes 130Z in the conversion of the fermentable sugar to lactic acid. The highest mannose concentration of 25.1 g/L was achieved at the optimum hydrolysis conditions of 4 hours and 3% (w/v) oxalic acid. By using immobilized cells with coconut shell-activated carbon (CSAC) of different sizes, 31.64 g/L of lactic acid was produced in the repeated batch cultivation.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Kimberley T. McLean, Alexandra Tikhomirova, Erin B. Brazel, Salome Legendre, Gian Haasbroek, Vikrant Minhas, James C. Paton, Claudia Trappetti
Summary: The pathogenicity of Streptococcus pneumoniae is enhanced by cell-cell signaling via the AI-2 system, leading to increased utilization of galactose as a carbon source and a hypervirulent phenotype. Phosphorylation of the transcriptional regulator GalR plays a critical role in pneumococcal colonization and transition to pathogenic lifestyle, affecting both the Leloir and tagatose 6-phosphate pathways.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Agricultural Engineering
Itziar A. Escanciano, Victoria E. Santos, Angeles Blanco, Miguel Ladero
Summary: In this study, succinic acid was anaerobically produced from potato waste and glucose using Actinobacillus succinogenes. A simple kinetic model was developed to accurately predict the evolution of succinic acid, substrate, by-products, and biomass. Experiments comparing fermentation performance between potato waste and pure glucose showed that using the hydrolysate from potato waste resulted in a 37% improvement in succinic acid yield. The proposed kinetic model was successfully applied to the fermentation data, making it a valuable tool for future techno-economic analysis of the bioprocess.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Engineering, Environmental
Ping Wu, Peng Ding, Chao Zhang, Hong-bo Liu, Chong-jun Chen, He Liu
Summary: This study explored the feasibility of using amino acids as alternative chemical electron donors to produce MCFAs, and proposed a novel two-stage electro-fermentation process. The results showed that this method can convert organic substrates into MCFAs through the synergistic action of microbes.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Zhennan Jiang, Zhiyong Cui, Ziwei Zhu, Yinghang Liu, Ya-jie Tang, Jin Hou, Qingsheng Qi
Summary: By screening mitochondrial carriers and C4-dicarboxylic acid transporters, efficient secretion of succinic acid in Yarrowia lipolytica was enhanced. The engineered strain PGC62-SYF-Mae showed high growth and succinic acid production, achieving a titer of 101.4 g/L, a productivity of 0.70 g/L/h, and a yield of 0.37 g/g glucose in fed-batch fermentation. Our study demonstrates that transporter engineering is a powerful strategy to promote industrial production of bio-based succinic acid.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Agricultural Engineering
Chao Xu, Md. Asraful Alam, Zhongming Wang, Yuande Peng, Chunliang Xie, Wenbing Gong, Qi Yang, Shushi Huang, Wei Zhuang, Jingliang Xu
Summary: By optimizing co-fermentation conditions and strategies, it is possible to efficiently extract succinic acid and ethanol from sugarcane bagasse, utilize glucose and xylose effectively, and reduce CO2 emissions.
BIORESOURCE TECHNOLOGY
(2021)
Article
Agricultural Engineering
Chao Xu, Yaru Xiong, Jun Zhang, Kuntai Li, Saiyi Zhong, Shushi Huang, Chunliang Xie, Wenbing Gong, Zuohua Zhu, Yingjun Zhou, Yuande Peng
Summary: This study applied response surface methodology to optimize the liquid hot water pretreatment of sugarcane bagasse, and achieved the highest sugar yield under the pretreatment conditions of 186°C, 25 min, and solid-to-liquid ratio of 0.08. By enzymatic hydrolysis of the pretreated residuals at 20% (w/v) solid content, the obtained sugars reached 121 g/L. Through controlling the fermentation process, most of the C5 and C6 sugars were converted into succinic acid with a high conversion rate of 280 mg/g SCB. This study provides a new clue for clean and efficient biorefining of chemicals.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Lin Dai, Zhina Lian, Rui Zhang, Ali Nawaz, Ikram ul Haq, Xin Zhou, Yong Xu
Summary: Efficiency of gluconic acid production from cellulosic materials can be improved through a combined strategy of activated carbon treatment and high-tension oxygen supply, enabling industrial-scale production.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Biochemical Research Methods
Tian Ma, Yuanjie Zhou, Xiaowei Li, Fayin Zhu, Yongbo Cheng, Yi Liu, Zixin Deng, Tiangang Liu
BIOTECHNOLOGY JOURNAL
(2016)
Article
Biochemistry & Molecular Biology
Fayin Zhu, Lei Lu, Shuai Fu, Xiaofang Zhong, Mengzhu Hu, Zixin Deng, Tiangang Liu
PROCESS BIOCHEMISTRY
(2015)
Article
Biochemical Research Methods
Tian Ma, Yuanjie Zhou, Xiaowei Li, Fayin Zhu, Yongbo Cheng, Yi Liu, Zixin Deng, Tiangang Liu
BIOTECHNOLOGY JOURNAL
(2016)
Article
Biotechnology & Applied Microbiology
Michael J. McAnulty, Venkata Giridhar Poosarla, Jine Li, Valerie W. C. Soo, Fayin Zhu, Thomas K. Wood
BIOTECHNOLOGY AND BIOENGINEERING
(2017)
Article
Biotechnology & Applied Microbiology
Ziling Ye, Xiaowei Li, Yongbo Cheng, Zhijie Liu, Gaoyi Tan, Fayin Zhu, Shuai Fu, Zixin Deng, Tiangang Liu
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Valerie W. C. Soo, Michael J. McAnulty, Arti Tripathi, Fayin Zhu, Limin Zhang, Emmanuel Hatzakis, Philip B. Smith, Saumya Agrawal, Hadi Nazem-Bokaee, Saratram Gopalakrishnan, Howard M. Salis, James G. Ferry, Costas D. Maranas, Andrew D. Patterson, Thomas K. Wood
MICROBIAL CELL FACTORIES
(2016)
Article
Biotechnology & Applied Microbiology
Fayin Zhu, Yuanshan Wang, Ka-Yiu San, George N. Bennett
BIOTECHNOLOGY AND BIOENGINEERING
(2018)
Article
Biotechnology & Applied Microbiology
Feng Qi, Chandresh Thakker, Fayin Zhu, Matthew Pena, Ka-Yiu San, George N. Bennett
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
Ran Liu, Fayin Zhu, Lei Lu, Aisi Fu, Jiankai Lu, Zixin Deng, Tiangang Liu
METABOLIC ENGINEERING
(2014)
Article
Biotechnology & Applied Microbiology
Fayin Zhu, Chengqiang Wang, Ka-Yiu San, George N. Bennett
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Fayin Zhu, Ka-Yiu San, George N. Bennett
BIOCHEMICAL ENGINEERING JOURNAL
(2020)
Article
Biotechnology & Applied Microbiology
Fayin Zhu, Matthew Pena, George N. Bennett
Summary: In this study, a high-efficiency quinolinic acid producer was constructed through comprehensive engineering strategies, including deactivating multiple key genes to increase production and using peptide-peptide interaction to enhance the production rate. These results lay the foundation for constructing efficient quinolinic acid-producing strains and converting it to nicotinic acid for industrial applications.
METABOLIC ENGINEERING
(2021)
Article
Multidisciplinary Sciences
Maren Nattermann, Sebastian Wenk, Pascal Pfister, Hai He, Seung Hwan Lee, Witold Szymanski, Nils Guntermann, Fayin Zhu, Lennart Nickel, Charlotte Wallner, Jan Zarzycki, Nicole Paczia, Nina Gaissert, Giancarlo Francio, Walter Leitner, Ramon Gonzalez, Tobias J. Erb
Summary: Researchers developed a two-enzyme route to activate formate into formyl phosphate and reduce it to formaldehyde, providing a potential tool for synthetic formate assimilation.
NATURE COMMUNICATIONS
(2023)
Article
Endocrinology & Metabolism
Alexander Chou, Seung Hwan Lee, Fayin Zhu, James M. Clomburg, Ramon Gonzalez
Summary: The study introduces an alternative approach using synthetic pathways for C1 bioconversion, generating multicarbon products directly from formate, formaldehyde and methanol while being orthogonal to the host metabolic network. Variants of the FORCE pathways were evaluated through thermodynamic and stoichiometric analyses, showing promising results both in vitro and in vivo. This synthetic metabolic pathway demonstrates the potential for C1 compound utilization and integration with host metabolism for synthetic methylotrophy.