Article
Engineering, Biomedical
Sean M. Kinney, Krystal Ortaleza, Alexander E. Vlahos, Michael Sefton
Summary: This study demonstrates that islet transplantation can be achieved in the subcutaneous space using MAA-PEG hydrogel, without the need for vascularizing cells or growth factors. The survival of islet grafts through the inflammatory events of hydrogel degradation and islet revascularization highlights the benefits of the MAA biomaterial.
Article
Engineering, Biomedical
Alexander E. Vlahos, Ilana Talior-Volodarsky, Sean M. Kinney, Michael V. Sefton
Summary: This study highlights a biomaterial-based approach using a methacrylic acid copolymer coating to generate a robust pre-vascularized subcutaneous cavity for islet transplantation. The pre-vascularized site was able to support a marginal mass of islets, rapidly returning streptozotocin-induced diabetic mice to normoglycemia.
Article
Multidisciplinary Sciences
Norifumi Kanai, Akiko Inagaki, Yasuhiro Nakamura, Takehiro Imura, Hiroaki Mitsugashira, Ryusuke Saito, Shigehito Miyagi, Kimiko Watanabe, Takashi Kamei, Michiaki Unno, Yasuhiko Tabata, Masafumi Goto
Summary: A novel bioabsorbable gelatin hydrogel nonwoven fabric (GHNF) can improve subcutaneous islet transplantation by compensating and protecting islet function through extracellular matrix and various growth factors, rather than enhanced neovascularization.
SCIENTIFIC REPORTS
(2023)
Article
Engineering, Biomedical
Shadab Abadpour, Essi M. Niemi, Linnea Strid Orrhult, Carolin Hermanns, Rick de Vries, Liebert Parreiras Nogueira, Havard Jostein Haugen, Dag Josefsen, Stefan Krauss, Paul Gatenholm, Aart van Apeldoorn, Hanne Scholz
Summary: This study reports the manufacturing of a 3D bioprinted double-layered scaffold using alginate/nanofibrillated cellulose (NFC) bioink to combine islets and adipose-derived stromal cells (ASCs) for beta cell replacement therapy. The results demonstrate that ASCs improve islet viability, glucose sensing, insulin secretion, and reduce pro-inflammatory cytokine secretion. Diabetic mice transplanted with islet-ASC scaffolds achieve normoglycemia and show elevated levels of C-peptide.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Jia-Pu Liang, Robert P. Accolla, Madhuvanthi Soundirarajan, Amy Emerson, Maria M. Coronel, Cherie L. Stabler
Summary: The use of OxySite microbeads incorporated within a macroporous PDMS scaffold allowed for sufficient local oxygenation for up to 20 days without toxic by-products. This approach showed promising potential for clinically relevant cell transplantation, highlighting the superior translational potential of off-the-shelf OxySite scaffolds for improving graft outcomes.
ACTA BIOMATERIALIA
(2021)
Article
Peripheral Vascular Disease
Patrick C. Hancock, Srinivas V. Koduru, Mingjie Sun, Dino J. Ravnic
Summary: Precision injury to recipient macrovasculature can promote neovessel formation in adjacently placed scaffolds. Microsurgical micropunctures on recipient vessels resulted in earlier cellular infiltration, increased physiologic perfusion, and enhanced capillary network formation in scaffolds.
MICROVASCULAR RESEARCH
(2021)
Article
Surgery
M. Honarpisheh, Y. Lei, Y. Zhang, M. Pehl, E. Kemter, M. Kraetzl, A. Lange, E. Wolf, L. Wolf-van Buerck, J. Seissler
Summary: The re-aggregation of single cells from dissociated NPICCs generates cell clusters with excellent functionality and improved in vivo function compared to native NPICCs.
TRANSPLANT INTERNATIONAL
(2022)
Article
Immunology
Shohei Takaichi, Yoshito Tomimaru, Takami Akagi, Shogo Kobayashi, Yasunari Fukuda, Keisuke Toya, Tadafumi Asaoka, Yoshifumi Iwagami, Daisaku Yamada, Hirofumi Akita, Takehiro Noda, Kunihito Gotoh, Yuichiro Doki, Mitsuru Akashi, Hidetoshi Eguchi
Summary: In this study, a vascularized beta-cell spheroid tissue was constructed using a layer-by-layer cell-coating technique and transplanted subcutaneously in a T1D model mouse. The results showed that this method could decrease blood glucose levels in diabetic mice, possibly due to host angiogenesis in the graft.
Article
Cell & Tissue Engineering
Kenyu Nakayama-Iwatsuki, Masumi Hirabayashi, Shinichi Hochi
Summary: The study demonstrates that pancreatic islet single cells prepared using different freezing methods can successfully generate pseudo-islets with normal functionality and appropriate size. These pseudo-islets exhibit sufficient response to high glucose stimulation and have a similar arrangement of endocrine cells as native islets.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Fatemeh Sabet Sarvestani, Ali-Mohammad Tamaddon, Ramin Yaghoobi, Bita Geramizadeh, Samira Sadat Abolmaali, Maryam Kaviani, Somayeh Keshtkar, Sara Pakbaz, Negar Azarpira
Summary: Diabetes is an autoimmune disease characterized by insufficient insulin production. Islet isolation using biocompatible scaffolds is a potential treatment strategy. This study aimed to investigate the effects of transfected BM-MSCs with angiomiR-126 and -210 on islet survival and functionality in a 3D scaffold with laminin. The results showed increased islet proliferation, viability, and insulin gene expression in the laminin-containing scaffolds, particularly in the miRNA-126 treated group.
CELL BIOCHEMISTRY AND FUNCTION
(2023)
Article
Transplantation
Alexandra M. Smink, Samuel Rodriquez, Shiri Li, Bryan Ceballos, Nicole Corrales, Michael Alexander, Taco Koster, Bart J. de Haan, Jonathan R. T. Lakey, Paul de Vos
Summary: This study developed and tested a polymeric subcutaneous transplantation site for repeated islet transplantation. The results showed that polycaprolactone (PCL) scaffolds were more stable and had less immune cell infiltration compared to poly(D,L,-lactide-co-epsilon-caprolactone) (PDLLCL) scaffolds. In pig experiments, allogenic islets demonstrated survival for a month under the skin in PCL scaffolds.
TRANSPLANTATION DIRECT
(2023)
Article
Gastroenterology & Hepatology
Sandeep Kumar, Daria Jach, Wendy Macfarlane, Tatjana Crnogorac-Jurcevic
Summary: By utilizing a rotary cell culture system, a simple in vitro 3D pancreatic model was established to study interactions between PDAC and islet beta cells. This system enabled the formation of 3D spheroids and showed PDAC cells' affinity to grow around and invade the pseudo islets, mimicking in vivo conditions.
Article
Chemistry, Multidisciplinary
Abdullah Muhammad Syed, Presley MacMillan, Jessica Ngai, Stefan Wilhelm, Shrey Sindhwani, Benjamin R. Kingston, Jamie L. Y. Wu, Pablo Llano-Suarez, Zachary Pengju Lin, Ben Ouyang, Zaina Kahiel, Suresh Gadde, Warren C. W. Chan
Article
Chemistry, Physical
Shrey Sindhwani, Abdullah Muhammad Syed, Jessica Ngai, Benjamin R. Kingston, Laura Maiorino, Jeremy Rothschild, Presley MacMillan, Yuwei Zhang, Netra Unni Rajesh, Tran Hoang, Jamie L. Y. Wu, Stefan Wilhelm, Anton Zilman, Suresh Gadde, Andrew Sulaiman, Ben Ouyang, Zachary Lin, Lisheng Wang, Mikala Egeblad, Warren C. W. Chan
Article
Materials Science, Multidisciplinary
Yih Yang Chen, Benjamin R. Kingston, Warren C. W. Chan
ADVANCED MATERIALS TECHNOLOGIES
(2020)
Article
Chemistry, Physical
Ben Ouyang, Wilson Poon, Yi-Nan Zhang, Zachary P. Lin, Benjamin R. Kingston, Anthony J. Tavares, Yuwei Zhang, Juan Chen, Michael S. Valic, Abdullah M. Syed, Presley MacMillan, Julien Couture-Senecal, Gang Zheng, Warren C. W. Chan
Review
Nanoscience & Nanotechnology
Wilson Poon, Benjamin R. Kingston, Ben Ouyang, Wayne Ngo, Warren C. W. Chan
NATURE NANOTECHNOLOGY
(2020)
Article
Engineering, Biomedical
Alexander E. Vlahos, Ilana Talior-Volodarsky, Sean M. Kinney, Michael V. Sefton
Summary: This study highlights a biomaterial-based approach using a methacrylic acid copolymer coating to generate a robust pre-vascularized subcutaneous cavity for islet transplantation. The pre-vascularized site was able to support a marginal mass of islets, rapidly returning streptozotocin-induced diabetic mice to normoglycemia.
Review
Medicine, General & Internal
Sara H. Keshavjee, Katherine J. P. Schwenger, Jitender Yadav, Timothy D. Jackson, Allan Okrainec, Johane P. Allard
Summary: Obesity is a major public health crisis, and bariatric surgery has become an important tool in addressing it and related conditions. While BS techniques have improved, there is no precise way to predict who will benefit the most. Understanding adipose tissue characteristics and other factors may help in identifying patients who will receive the greatest benefit.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Surgery
Meghan Aversa, Tereza Martinu, Christopher Patriquin, Marcelo Cypel, David Barth, Rasheed Ghany, Jin Ma, Shaf Keshavjee, Lianne G. Singer, Kathryn Tinckam
Summary: The Toronto Lung Transplant Program has been using a desensitization regimen since 2008 for DSA-positive lung transplants, and long-term follow-up showed that allograft survival and CLAD-free survival of these patients were not significantly different from other lung transplant recipients.
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Article
Engineering, Biomedical
Sean M. Kinney, Krystal Ortaleza, Alexander E. Vlahos, Michael Sefton
Summary: This study demonstrates that islet transplantation can be achieved in the subcutaneous space using MAA-PEG hydrogel, without the need for vascularizing cells or growth factors. The survival of islet grafts through the inflammatory events of hydrogel degradation and islet revascularization highlights the benefits of the MAA biomaterial.
Article
Multidisciplinary Sciences
Alexander E. Vlahos, Jeewoo Kang, Carlos A. Aldrete, Ronghui Zhu, Lucy S. Chong, Michael B. Elowitz, Xiaojing J. Gao
Summary: The authors have developed a modular approach called RELEASE to control intercellular signals using protein-based circuits. This design allows for diverse signal processing capabilities and has the potential to enable programmable delivery of intercellular cues in various fields such as neurobiology, cancer immunotherapy, and cell transplantation.
NATURE COMMUNICATIONS
(2022)
Review
Oncology
Sara H. Keshavjee, Ryan H. Moy, Steven L. Reiner, Sandra W. Ryeom, Sam S. Yoon
Summary: Gastric cancer is a lethal form of cancer usually arising from the inner mucosa layer of the stomach. Surgery and chemotherapy are the primary treatments, but immunologic therapies have shown promise in the treatment of gastric cancer. This review summarizes the role of the immune system in gastric cancer formation and growth, as well as the current research on immunologic therapies.
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.