Article
Green & Sustainable Science & Technology
Youqian Xiao, Hongnan Yang, Dan Zheng, Yi Liu, Cong Zhao, Liangwei Deng
Summary: The study found that protein degradation decreased with increasing ammonia concentration at 20 degrees Celsius, but was less affected at 35 degrees Celsius and 55 degrees Celsius. Additionally, adding GAC at different temperatures significantly improved methane production rates.
Article
Multidisciplinary Sciences
Rodrigo Sequeda Barros, Michel Duran Contreras, Felipe Romani Morris, Marley Vanegas Chamorro, Alberto Albis Arrieta
Summary: Waste management technologies now offer opportunities to generate value-added products. Anaerobic digestion has been identified as an effective method to produce biogas from organic wastes. This study evaluated the potential of methane production through anaerobic digestion of rumen and chicken manure, two common agroindustrial wastes from the Colombian Caribbean region. The results showed that thermophilic digestion produced more biogas, while mesophilic digestion had better biogas quality. The study also found that anaerobic digestion of rumen with added fat-oil-grease (FOG) under mesophilic conditions performed exceptionally well in terms of biogas quantity and quality.
Article
Biotechnology & Applied Microbiology
Liu Cao, Chris D. Cox, Qiang He
Summary: This study identified the important role of Syntrophobacter in syntrophic propionate conversion, while the methanogen partners lacked consistency. Propionate conversion was mainly achieved through the transformation of propionate into acetate and hydrogen.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Engineering, Chemical
James Naphtali, Alexander W. Y. Chan, Faizan Saleem, Enze Li, Jacob Devries, Herb E. Schellhorn
Summary: This study examined the potential of high-throughput DNA sequencing as a tool for taxonomic and functional profiling of microbial communities in anaerobic digesters. The results showed that different designs of anaerobic digesters had distinct microbial communities and functional profiles. These findings suggest that high-throughput DNA sequencing can be used as a monitoring tool to study microbial community changes in on-site wastewater treatment systems.
Article
Engineering, Environmental
Qinxue Wen, Shuo Yang, Zhiqiang Chen
Summary: The study found that sulfamethoxazole had a significant impact on the microbial community and resistance genes, while norfloxacin had little influence. Thermophilic anaerobic digestion showed higher methane production potential and greater reduction in resistance gene abundance.
FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING
(2021)
Article
Agricultural Engineering
Fangyi Tang, Jun Tian, Nengwu Zhu, Yunqin Lin, Haomin Zheng, Zhiyong Xu, Weizhen Liu
Summary: This study investigates the influence of the mixing ratio of ammoniated straw to biogas residue on the stability and methane yield of dry anaerobic digestion and analyzes the structure of the microbial community over time. The results show that the reactor with a ratio of 3:3 yielded the highest methane production, with bacteria being the dominant species. Methanosarcina and acid-tolerant Methanobacterium played important roles in anaerobic digestion and methane production.
BIORESOURCE TECHNOLOGY
(2022)
Article
Environmental Sciences
Fengping Hu, Shihao Zhang, Susu Liu, Liping Wan, Guijin Gong, Tengfang Hu, Xin Wang, Li Xu, Gaoping Xu, Yuying Hu
Summary: This study investigates the acidulated swine manure anaerobic digestion (AD) with bentonite supplementation. Results show that bentonite addition can alleviate acid inhibition, improve gas production performance, and enhance microbial mechanism. The methane production rate in the bentonite-added digester is 2.87 times higher than that of the control digester, and the chemical oxygen demand (COD) removal rate is increased by 140.1% via bentonite supplementation. Microbial community analysis reveals the dominant methanogenesis pathway and the presence of syntrophic acetate oxidation (SAO) bacteria. This study provides guidance for the practical biogas project.
Article
Agricultural Engineering
Ying Li, Changrui Wang, Xinrui Xu, Yongming Sun, Tao Xing
Summary: Introducing propionate-degrading methanogenic cultures can enhance methane yield in anaerobic digesters under high ammonia levels, improving the performance of anaerobic digestion.
BIORESOURCE TECHNOLOGY
(2022)
Article
Thermodynamics
Youqian Xiao, Hongnan Yang, Dan Zheng, Yi Liu, Liangwei Deng
Summary: The effects of granular activated carbon (GAC) and organic load rate (OLR) on semi-continuous dry anaerobic digestion of swine manure were investigated. The study found that dry anaerobic digestion of swine manure has a good methane production rate and application prospects under appropriate OLR. As OLR increases, the ammonia nitrogen concentration initially increases and then decreases, with the addition of GAC contributing to improved biogas production efficiency.
Article
Environmental Sciences
He Linsong, Li Lianhua, Li Ying, Wang Changrui, Sun Yongming
Summary: The study showed that bioaugmentation increased methane yield and shortened methane production time in batch reactors. Higher bioaugmentation doses did not significantly improve efficiency, with a recommended dosage of 0.27 g VSBS/g VSCM. Additionally, whole genome pyrosequencing revealed changes in the dominant methanogenic pathways with bioaugmentation, indicating enhanced metabolic activities in the digestor.
Article
Agricultural Engineering
Zhijiang Shao, Xiaohui Guo, Qiang Qu, Kang Kang, Qiyuan Su, Chao Wang, Ling Qiu
Summary: Residual chlorine disinfectants can inhibit anaerobic digestion, particularly in the initial stage of mesophilic conditions. Inhibitory effects were reduced in thermophilic conditions, but a decrease in CMPu was observed later. Microbial analysis revealed enrichment of chlorine-resistant bacteria and archaea due to the presence of residual chlorine disinfectants.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Gede Adi Wiguna Sudiartha, Tsuyoshi Imai, Chonticha Mamimin, Alissara Reungsang
Summary: Temperature plays a significant role in anaerobic digestion, affecting microbial communities and process efficiency. This study tested two temperature shift scenarios and found that both led to a decrease in biogas production, with the upshifted scenario experiencing a larger decrease. 16S rRNA sequencing revealed that the dominant methanogens differed between the two scenarios, indicating that temperature shifts affect microbial communities and biogas production.
FERMENTATION-BASEL
(2023)
Article
Agricultural Engineering
Shichao Zheng, Fei Yang, Wenli Huang, Zhongfang Lei, Zhenya Zhang, Weiwei Huang
Summary: The combined effect of ZVI and magnetite on semi-dry anaerobic digestion of swine manure was studied. The results showed that both ZVI and magnetite increased methane production by altering the metabolism pathways. ZVI promoted interspecies hydrogen transfer, while magnetite accelerated organic hydrolysis and established direct interspecies electron transfer.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biology
Shichun Ma, Fan Jiang, Yan Huang, Yan Zhang, Sen Wang, Hui Fan, Bo Liu, Qiang Li, Lijuan Yin, Hengchao Wang, Hangwei Liu, Yuwei Ren, Shuqu Li, Lei Cheng, Wei Fan, Yu Deng
Summary: This study provides insights into the microbial composition and function in anaerobic digestion (AD), showing the influence of feedstock type on carbohydrate hydrolysis and methanogenesis. Additionally, a large number of reference genome and gene resources were identified for analysis of anaerobic microbiota.
Article
Agricultural Engineering
Lifei Zou, Changmei Wang, Xingling Zhao, Kai Wu, Chengyue Liang, Fang Yin, Bin Yang, Jing Liu, Hong Yang, Wudi Zhang
Summary: Microbial electrolysis cell coupled anaerobic digestion (MEC-AD) is an effective technology for recycling swine manure, with the optimal voltage of 0.9 V resulting in maximum biogas and methane yields. The use of BP-ANN model proved superior in studying cumulative yields and establishing a model for enhanced gas production and accelerated substrate degradation.
BIORESOURCE TECHNOLOGY
(2021)
Article
Plant Sciences
Xiu-li Liu, Lan Wang, Xiao-wen Wang, Yan Yan, Xiao-li Yang, Meng-yang Xie, Zhi Hu, Xing Shen, Hao Ai, Hong-hui Lin, Guo-hua Xu, Jian Yang, Shu-bin Sun
Article
Chemistry, Analytical
Lan Wang, Jun Wang, Qing Chen, Jian Yang, Liangwei Deng, Zhen Huang
Summary: A new direct mRNA multiplexed detection strategy based on xMAP array has been developed to analyze gene expression without reverse transcription and amplification steps, showing great potential in environmental microbial detection and activity diagnosis of wastewater treatment plants. The technology enables high sensitivity and wide dynamic range detection, simplifying the process and providing accurate results.
MICROCHEMICAL JOURNAL
(2021)
Article
Agricultural Engineering
Hongnan Yang, Liangwei Deng, Jianwang Wu, Wenguo Wang, Dan Zheng, Zhiyong Wang, Yi Liu
Summary: The study found that the appropriate conditions for air mixing in anaerobic digestion were 66.7 mL air per volume of reactor per min with a mixing time of 1.5 min. Exceeding 3 min of air mixing time led to a decrease in total CH4 production. The feasibility of air mixing in a full-scale anaerobic plant was validated through one year of operating data with swine wastewater.
BIORESOURCE TECHNOLOGY
(2021)
Article
Green & Sustainable Science & Technology
Youqian Xiao, Hongnan Yang, Dan Zheng, Yi Liu, Cong Zhao, Liangwei Deng
Summary: The study found that protein degradation decreased with increasing ammonia concentration at 20 degrees Celsius, but was less affected at 35 degrees Celsius and 55 degrees Celsius. Additionally, adding GAC at different temperatures significantly improved methane production rates.
Article
Environmental Sciences
Wen-yan Wang, Ru Wang, Ghulam Abbas, Gang Wang, Zhi-guo Zhao, Liang-wei Deng, Lan Wang
Summary: This study investigates the effects of aggregation on the activity, growth, and reproduction of anammox bacteria. Results show that aggregation benefits the bacteria by enhancing their activity, relative abundance, growth, and reproduction rate.
Article
Thermodynamics
Youqian Xiao, Hongnan Yang, Dan Zheng, Yi Liu, Liangwei Deng
Summary: The effects of granular activated carbon (GAC) and organic load rate (OLR) on semi-continuous dry anaerobic digestion of swine manure were investigated. The study found that dry anaerobic digestion of swine manure has a good methane production rate and application prospects under appropriate OLR. As OLR increases, the ammonia nitrogen concentration initially increases and then decreases, with the addition of GAC contributing to improved biogas production efficiency.
Article
Engineering, Environmental
Hong Wang, Peike Wu, Dan Zheng, Liangwei Deng, Wenguo Wang
Summary: N-acyl-homoserine lactones (AHLs) play an important role in quorum sensing regulation of Gram-negative bacteria, positively influencing the production of aromatic proteins in extracellular polymeric substances (EPSs). Addition of exogenous C12-HSL enhances flocculation activity and aromatic protein production in a Chlorella-bacteria system, while the quorum sensing inhibitor vanillin suppresses these effects.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Energy & Fuels
Gangjin Liu, Yi Liu, Balazs Franko, Hongnan Yang, Dan Zheng, Liangwei Deng, Jing Liu
Summary: In this study, an energy balance model and Monte Carlo simulation were used to analyze the probability of achieving energy self-sufficiency in three CAO systems. The results showed that the MPC-CAO system had the highest probability and stability of energy self-sufficiency under uniformly distributed input parameters. The ambient temperature was found to be the main factor affecting the net energy production of the CAO system, but its effect could be reduced by optimizing control parameters and increasing organic matter capture efficiency. Additionally, capturing more organic matter and nitrogen was highly beneficial for achieving energy self-sufficiency in the CAO systems.
Article
Environmental Sciences
Han Yang, Liangwei Deng, Hongnan Yang, Youqian Xiao, Dan Zheng
Summary: This study found that with sodium acetate as the organic carbon source in the zero-valent iron-mediated nitrogen removal system operated in co-substrate mode, the removal efficiency of NH4+-N and TIN could be significantly improved. The organic matter not only stimulated the activity of ammonia oxidation and denitrification, but also reduced the toxicity of iron to microorganisms, leading to the enrichment of certain bacteria.
Article
Agricultural Engineering
Xinwei Li, Liangwei Deng, Fucheng Li, Dan Zheng, Hongnan Yang
Summary: This study aimed to investigate the effect of air mixing on high-solids anaerobic digestion system performance and understand the mechanisms behind it. The results showed that the methane yield increased when different air mixing intensities were applied. The increase in performance was attributed to improved hydrolysis rate and carbon recovery, as well as the abundance of certain microorganisms and enzymes under air mixing condition.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Long -Xing Jiang, Lu Guo, James P. Shapleigh, Yi Liu, Yan Huang, Jin-Shi Lian, Ling Xie, Liang-Wei Deng, Wen-Guo Wang, Lan Wang
Summary: A 160-day incubation was conducted to investigate the effect of glutaraldehyde on anammox bacteria. The results showed that the nitrogen removal efficiency significantly decreased to 11% in the GA reactor when the glutaraldehyde concentration reached 40 mg/L, which was only one-quarter of that in the CK reactor. Glutaraldehyde caused the dissociation of anammox bacteria from granules and led to the succession of denitrifier community. Metagenome analysis revealed the replacement of denitrifiers with different efflux pumps and the lack of NodT proteins in a specific strain.
BIORESOURCE TECHNOLOGY
(2023)
Review
Environmental Sciences
Liangwei Deng, Dan Zheng, Jingni Zhang, Hongnan Yang, Lan Wang, Wenguo Wang, Ting He, Yunhong Zhang
Summary: The management of swine wastewater in the farming industry is focused on the disposal mode, including treated waste application and discharge standard treatment. Various unit technologies for treatment and utilization have been reviewed, such as solid-liquid separation, aerobic treatment, anaerobic treatment, digestate utilization, natural treatment, anaerobic-aerobic combined treatment, and advanced treatment. Different technologies are suitable for different types and sizes of pig farms based on land availability and treatment requirements, and there are challenges in winter operation, digestate utilization, and treatment cost for meeting discharge standards.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Engineering, Environmental
Ting He, Liangwei Deng, Bo Lai, Shuguang Xu, Lan Wang, Yunhong Zhang, Dan Zheng, Changwei Hu
Summary: Fulvic acid, as a model representing bio-refractory soluble organic matter, was treated with a combination of FeSO4 and Ca(ClO)(2) in advanced wastewater treatment. The results showed that the synergistic effect of Fe2+ and ClO- greatly enhanced the removal efficiency of fulvic acid, indicating the involvement of an advanced oxidation process. Oxidation and coagulation played crucial roles in the removal of fulvic acid.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Engineering, Environmental
Yunhong Zhang, Lan Wang, Zhaokun Xiong, Wenguo Wang, Dan Zheng, Ting He, Yi Liu, Yi Ran, Liangwei Deng, Bo Lai
CHEMICAL ENGINEERING JOURNAL
(2020)
Article
Biotechnology & Applied Microbiology
Yiran Qu, Innocent Bekard, Ben Hunt, Jamie Black, Louis Fabri, Sally L. Gras, Sandra. E. Kentish
Summary: This study compares the performance of a nanofiber device and a resin column for antibody capture. The nanofiber device has a larger housing volume and lower binding capacity, but comparable eluate purity to the resin column. It shows high stability, can be used for multiple cycles, and maintains consistent eluate quality when scaled up. The use of a single nanofiber device can significantly reduce costs compared to a resin column, especially when the number of batches is limited.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Brandon Moore, Christos Georgakis, Chris Antoniou, Sarwat Khattak
Summary: Fed-batch cell culture processes are commonly used in biomanufacturing due to their simplicity and applicability in cGMP environments. However, the challenge lies in the changing physiochemical conditions within the bioreactor as the cell density changes. Traditional response surface models (RSMs) are commonly used for optimization but are limited by their use of time-invariant factors. Dynamic RSM (DRSM) models can predict the time-dependent impact of process inputs, allowing for optimization of process operations that change over time.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lin Li, Yunfan Bai, Chuhua Qi, Yile Du, Xiaoxiao Ma, Yutong Li, Pingping Wu, Shuangli Chen, Sijing Zhang
Summary: A succinic anhydride-modified apple pomace (SAMAP) was synthesized to address environmental issues caused by the accumulation of apple pomace and effectively treat heavy metal ions. SAMAP exhibited high adsorption capacity for Cu(II) and Pb(II), suggesting its potential application in wastewater treatment.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Peter Satzer
Summary: Water for injection (WFI) production in the biopharmaceutical industry consumes excessive amounts of water and energy. Recycling buffers can potentially save up to 90% of resources, but achieving the full theoretical potential is impossible when a risk-aware design is used. Universal risk-based assessment is important for regulatory authorities to consider the implementation of such a strategy.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Gaoya Sun, Lingkai Jin, Guangxue Wang, Xiaoge Wang, Jin Huang
Summary: In this study, heterologous expression and homologous overexpression of ABC transporter proteins AatA and MdlB were found to improve butyric acid production in C. tyrobutyricum. The overexpression of these proteins upregulated the expression levels of key enzymes in the acetate synthesis pathway and promoted the synthesis and secretion of acetic acid. Additionally, the increase in ATPase activity facilitated sugar utilization, induced extracellular secretion of acetate, and shortened fermentation periods.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Taiki Okamura, Rina Aritomi, Takuya Matsumoto, Ryosuke Yamada, Hidehiko Hirakawa, Hiroyasu Ogino
Summary: In this study, proline was introduced to improve the stability of putidaredoxin reductase (PdR) in the Pseudomonas putida cytochrome P450 system. It was found that PdR_T221P had a longer half-life at high temperatures compared to wild-type PdR, but a shorter half-life in the presence of methanol. Molecular dynamics simulations supported these findings.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yuying Hu, Xiaofan Wang, Shihao Zhang, Zimu Liu, Tengfang Hu, Xin Wang, Xiaoming Peng, Hongling Dai, Jing Wu, Fengping Hu
Summary: This study investigates the effect of iron-carbon micro-electrolysis (ICME) materials on high-solid anaerobic digestion (HSAD). The results show that ICME materials promote methane production in HSAD by increasing the attachment area of microorganisms and facilitating symbiotic metabolism of certain bacterial species. This study provides new insights into microbial mechanisms and enhances our understanding of ICME material enhancement in HSAD.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jibao Liu, Yufeng Xu, Yuansong Wei
Summary: This study investigated the role of sludge rheology in anaerobic digestion (AD) and found that rheological properties increased with the increase of solid content, resulting in a negative effect on methane production. An extended ADM1 model revealed that enhanced sludge rheological properties increased mass diffusion resistance and reduced uptake rate of acetate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Xiaoyan Liu, Zongze Chen, Dewen Kong, Xinying Zhang, Chuanhua Wang, Yongqi Wang
Summary: This study explored the role of intracellular and extracellular enzymes of Acinetobacter baumannii and Talaromyces sp. in the degradation of crude oil. The extracellular enzymes of Talaromyces sp. were more effective in degrading n-alkanes, while those of Acinetobacter baumannii had a better effect on aromatic hydrocarbons. The degradation enzyme systems of both bacteria and fungi complemented each other, improving the overall degradation ability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jing Dong, Lingli Xu, Yuxiang Liu, Li Ren, Ke Yuan
Summary: The utilization of biochar-immobilized microorganisms is an effective method for eliminating phenol from water. The high susceptibility of bacteria to environmental factors is a challenge for practical implementation. In this study, biochar was used to reduce microbial susceptibility and enhance phenol removal. The addition of biochar altered the dominant species of phenol-degrading bacteria and response surface analysis indicated the significant influence of biochar pyrolysis temperature and experimental temperature on phenol removal rate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Zibin Pan, Mengying Liu, Zuliang Chen
Summary: This study successfully removed metalloids and heavy metals from acid mine drainage (AMD) using bio-synthesized Fe/Cu nanoparticles (Fe/Cu NPs). The Fe/Cu NPs showed high removal capacities and the presence of organic substances contributed to their stability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Guangbing Liu, Han Zhang, Jincan Huang, Lu Zhang, Teng Zhang, Xuemin Yu, Weijing Liu, Chunkai Huang
Summary: This study investigated the effect of Fenton pre-treatment on the treatment efficiency of printing and dyeing wastewater (PDW) using two anaerobic/aerobic-membrane bioreactors (A/O-MBRs). The results showed that Fenton pre-treatment significantly improved the removal efficiency of COD and AOX in PDW, and reduced membrane fouling. The Shannon indices and metagenomics analysis indicated that the microbial diversity in anaerobic flocs was higher than that in aerobic flocs, and EC3.1.1.45 and pcaI were identified as key functional genes.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lidia Borgosz, Duygu Dikicioglu
Summary: The Industrial Internet of Things (IIoT) is a system that connects devices and provides real-time insight into industrial processes. However, the complexity and regulatory requirements of the biomanufacturing sector make it challenging to implement IIoT. There is a need for universal solutions to overcome this challenge and advance the field of biomanufacturing.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yajie Li, Weikang Kong, Yuyao Zhang, Huarui Zhou, Hongbo Liu, Salma Tabassum
Summary: In this study, the iron-carbon multi-micro electric field coupling anaerobic co-digestion technique was used to treat coal gasification wastewater (CGW). The experimental results showed that under optimal operating conditions, this technique can significantly reduce the toxicity of the wastewater and achieve high removal efficiencies. Additionally, the analysis of microbial communities revealed that the coupling system promotes direct interspecies electron transfer.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Aparecido Nivaldo Modenes, Debora Gozzi Fernandes, Daniela Estelita Goes Trigueros, Matheus Guilherme Amador, Fernando Rodolfo Espinoza-Quinones, Taysa de Souza Braniz, Adilson Ricken Schuelter, Glacy Jaqueline da Silva, Lucimar Pereira Bonett
Summary: This study aimed to systematically remove organic pollutants from raw dairy wastewater with high concentrations of COD, TOC, and TN using Poterioochromonas malhamensis algae strains. The results showed that the biomass yield rate using FP-PBRs was 10% higher than tubular PBRs, and the organic pollution in wastewater was significantly reduced with a decrease of about 98% in COD, 95% in TN, and 92% in TOC.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
