Journal
ANALYTICAL CHEMISTRY
Volume 92, Issue 1, Pages 1114-1121Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b04177
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Funding
- Project of the National Natural Science Foundation of China [21577170, 31570037]
- Key Project of Inter-Governmental International Scientific and Technological Innovation Cooperation [2016YFE0108900]
- National Key Research and Development Program of China [2016YFD0800606]
- National Institute of Environmental Health Sciences Superfund Research Program [P42ES04699]
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Bacterial magnetic particles (BMPs) are an attractive carrier material for immunoassays because of their nanoscale size, dispersal ability, and membrane-bound structure. Antitetrabromobisphenol-A (TBBPA) nanobodies (Nbs) in the form of monovalence (Nb1), bivalence (Nb2), and trivalence (Nb3) were biotinylated and immobilized onto streptavidin (SA)-derivatized BMPs to construct the complexes of BMP-SA-Biotin-Nb1, -Nb2, and -Nb3, respectively. An increasing order of binding capability of BMP-SA-Biotin-Nb1, -Nb2, and-Nb3 to TBBPA was observed. These complexes showed high resilience to temperature (90 degrees C), methanol (100%), high pH (12), and strong ionic strength (1.37 M NaCl). A BMP-SA-Biotin-Nb3-based enzyme linked immunosorbent assay (ELISA) for TBBPA dissolved in methanol was developed, showing a half-maximum inhibition concentration (IC50) of 0.42 ng mL(-1). TBBPA residues in landfill leachate, sewage, and sludge samples determined by this assay were in a range of
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