Journal
ANALYTICAL CHEMISTRY
Volume 92, Issue 4, Pages 3403-3408Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b05486
Keywords
-
Categories
Funding
- National Key Research and Development Program of China [2016YFA0501401, 2016YFA0501402, 2017YFA0505003]
- National Natural Science Foundation of China [:21974023, 21475027]
Ask authors/readers for more resources
An aminophenylboronic acid (APBA)-functionalized thorny-trap-shaped monolayer microarray as a 3D fractal structures' substrate was fabricated to capture and release circulating tumor cells (CTCs) efficiently. The microarray can not only trap cells inside by microbowls but also enhance the interaction between cell and substrate by providing more binding sites and facilitating the spread of cell filopodias via the growth of nanorods. Modification of APBA enhanced the interaction further by binding with sialic acid of CTCs surface. The special topological structure achieved a high capture efficiency of 79.5%. The captured cancer cells were released without introducing any affinity molecules by a ligand exchange reaction with up to 70% efficiency and good proliferation. This substrate can isolate 33 tumor cells from a mimic blood sample even at a low spiked number of 50 cancer cells. This study provides valuable guidance for isolation and release of CTCs and is significant for the further study of tumors.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available