Article
Cell & Tissue Engineering
Fengyi Liu, Xu Wang, Yuzhou Li, Mingxing Ren, Ping He, Lu Wang, Jie Xu, Sheng Yang, Ping Ji
Summary: In this study, a photocrosslinked GelMA/PAMAM-MA hydrogel was developed as a stem cell carrier for cartilage repair. The hybrid hydrogel showed improved structural and mechanical properties compared to GelMA alone, and demonstrated a sufficient ability to promote cartilage regeneration in both in vitro and in vivo experiments. This study provides new insight into the design strategy of stem cell delivery scaffolds.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Mohammad B. Aljaber, Fiona Verisqa, Zalike Keskin-Erdogan, Kapil D. Patel, David Y. S. Chau, Jonathan C. Knowles
Summary: Approximately half of an adult human's body weight is made up of muscles. The source and bloom number of gelatin have been found to have an impact on the mechanical properties and biological activities of GelMA hydrogels.
Article
Chemistry, Medicinal
Sasinan Bupphathong, Carlos Quiroz, Wei Huang, Pei-Feng Chung, Hsuan-Ya Tao, Chih-Hsin Lin
Summary: This review summarizes the recent modifications of GelMA and its applications in vascular tissue engineering and wound healing. Modified GelMA demonstrates improved mechanical properties and cell migration, stimulating vascular development and forming a structure comparable to the natural extracellular matrix.
Article
Nanoscience & Nanotechnology
He Wang, Bo Hu, Hong Li, Ge Feng, Shengyuan Pan, Ziqi Chen, Bo Li, Jinlin Song
Summary: In this study, hydroxyapatite nanofibers (HANFs) were incorporated into GelMA hydrogel to improve its mechanical properties and bone regenerative ability. The results showed that a certain amount of mineralized nanofibers significantly improved the biocompatibility and bone repair effect of the composite hydrogel, but increasing the amount of nanofibers did not lead to further improvement in performance.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Chemistry, Analytical
Qian Liang, Xiao Yu, Xie Chen, Qiang Huang, Tao Sun
Summary: This paper proposes a force-control-based cyclic loading method for rapidly evaluating the elastic modulus of microfibers with different GelMA concentrations. The method applies a small sinusoidal strain to the microfiber and fits the stress response into a sinusoidal wave to determine the maximum tensile stress and tensile strain. Compared to the commonly used constant force loading method, the proposed method demonstrates a significant advantage in measurement accuracy for low-concentration GelMA microfibers.
Article
Biochemistry & Molecular Biology
Hang Dong, Jian Li, Xueyuan Huang, Haiting Liu, Rong Gui
Summary: This study developed a novel multifunctional hydrogel, Pltm@CNPs/Gel, which incorporates platelet membrane camouflaged cerium nanoparticles into a gelatin methacryloyl matrix. The Pltm@CNPs/Gel hydrogel possesses anti-oxidative and anti-inflammatory properties and accelerates diabetic wound healing by promoting neovascularization, cell proliferation, and collagen fiber organization. The hydrogel shows potential as a therapeutic option for diabetic wound healing and as a diabetic wound dressing candidate.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Engineering, Biomedical
Qingyuan Zhang, Ting Yang, Ruitao Zhang, Xi Liang, Ge Wang, Yuan Tian, Li Xie, Weidong Tian
Summary: Incorporating human platelet lysate and nanoclay Laponite into GelMA microsphere system successfully improved the Young's modulus of the hybrid hydrogel, promoted cell migration and tubule formation, and ultimately facilitated vascularized endodontic regeneration.
ACTA BIOMATERIALIA
(2021)
Article
Chemistry, Multidisciplinary
Ji-Bong Choi, Yu-Kyoung Kim, Seon-Mi Byeon, Jung-Eun Park, Tae-Sung Bae, Yong-Seok Jang, Min-Ho Lee
Summary: The study involved fabricating a composite hydrogel by incorporating BCP into GelMA, resulting in a material with excellent mechanical properties, high cell viability, and strong bone differentiation ability for bone regeneration. The hydrogel also offers advantages for clinical use due to its customizable structure.
Article
Polymer Science
Wan-Chun Chang, Au-Zou Tai, Nian-Yun Tsai, Yi-Chen Ethan Li
Summary: The study synthesized an injectable and photo-crosslinkable bioadhesive made of gelatin methacryloyl and phenyl isothiocyanate-modified gelatin, offering good hemostatic properties and adjustable mechanical properties.
Article
Engineering, Biomedical
Hsuan Chen, Yuan-Min Lin, Sasinan Bupphathong, Joshua Lim, Jing-En Huang, Wei Huang, Tiffany Angela S. Hsieh, Chih-Hsin Lin
Summary: This study investigates the effects of incorporating silanized 45S5 bioactive glass into GelMA hydrogel and demonstrates the improved bioactivity and mechanical properties compared to traditional bioactive glass, indicating the potential application of silanization of bioactive glass in bone tissue engineering.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Review
Biotechnology & Applied Microbiology
Gwang-Bum Im, Ruei-Zeng Lin
Summary: Gelatin methacrylate (GelMA) hydrogels have excellent biocompatibility and biodegradability, making them widely used in tissue engineering and regenerative medicine. The mechanical characteristics of GelMA hydrogels can be adjusted by changing crosslinking conditions, allowing them to closely resemble the native extracellular matrix (ECM) properties. GelMA hydrogels have applications in bioengineering human vascular networks, both in vitro and in vivo. They can be used for disease modeling, drug screening, and ensuring oxygenation of tissue-engineered constructs. GelMA hydrogels can also be used as implantable materials for delivering therapeutic cells to rebuild vasculature in ischemic wounds.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Longwei Li, Muxin Yue, Qingyu Peng, Xiong Pu, Zheng Li
Summary: This study developed a zwitterionic hydrogel to promote osteogenic differentiation of BMSCs and enhance bone defect repair. The zwitterionic ECM-dependent metabolic response was found to be the underlying mechanism of the promoted osteogenic differentiation of BMSCs and bone regeneration.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Ryoma Goto, Eisaku Nishida, Shuichiro Kobayashi, Makoto Aino, Tasuku Ohno, Yuki Iwamura, Takeshi Kikuchi, Jun-ichiro Hayashi, Genta Yamamoto, Masaki Asakura, Akio Mitani
Summary: The study found that visible-light-crosslinked GelMA hydrogels, along with encapsulated osteoblasts, exhibited good mechanical and biological properties, making them suitable for use as scaffolds for bone regeneration. Osteoblasts encapsulated in GelMA hydrogels photopolymerized with visible light showed higher cell viability. Additionally, GelMA-RF hydrogels promoted osteoblast differentiation and have the potential to treat bone-destructive diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nanoscience & Nanotechnology
Zhe Shi, Qiang Zhong, Yuhang Chen, Jian Gao, Xin Pan, Qiang Lian, Rong Chen, Pinkai Wang, Jian Wang, Zhanjun Shi, Hao Cheng
Summary: This study developed a bone-mimetic injectable GelMA-HAP-SN system loaded with MSCs, HAP, and SN, showing promising osteogenic capability. Experimental results demonstrated that the GelMA-HAP-SN hydrogel exhibited excellent performance in terms of cellular viability, proliferation, and osteogenic biomarker expression.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Review
Biotechnology & Applied Microbiology
Yang-Hee Kim, Jonathan Dawson, Richard O. C. Oreffo, Yasuhiko Tabata, Dhiraj Kumar, Conrado Aparicio, Isha Mutreja
Summary: Musculoskeletal disorders pose a significant burden on the global economy and public health. Hydrogels, especially Gelatin methacryloyl (GelMA) hydrogel, hold great potential for enhancing the repair of damaged musculoskeletal tissues. This review provides a comprehensive overview of GelMA synthesis, hydrogel design, and its applications in regenerative medicine, particularly in the field of musculoskeletal drug delivery.
BIOENGINEERING-BASEL
(2022)
Article
Materials Science, Biomaterials
Pankaj Dwivedi, Shuya Han, Farhana Mangrio, Rong Fan, Monika Dwivedi, Zhigiang Zhu, Fangsheng Huang, Qiang Wu, Renuka Khatik, David E. Cohn, Ting Si, Shuiying Hu, Alex Sparreboom, Ronald X. Xu
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2019)
Article
Materials Science, Biomaterials
Wenqiang Du, Sungmin Hong, Giorgia Scapin, Marie Goulard, Dhvanit I. Shah
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2019)
Correction
Materials Science, Biomaterials
Pankaj Dwivedi, Shuya Han, Farhana Mangrio, Rong Fan, Monika Dwivedi, Zhiqiang Zhu, Fangsheng Huang, Qiang Wu, Renuka Khatik, David E. Cohn, Ting Si, Shuiying Hu, Alex Sparreboom, Ronald X. Xu
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2020)
Article
Nanoscience & Nanotechnology
Pankaj Dwivedi, Sonia Kiran, Shuya Han, Monika Dwivedi, Renuka Khatik, Rong Fan, Farhana Akbar Mangrio, Kun Du, Zhiqiang Zhu, Chaoyu Yang, Fangsheng Huang, Aslam Ejaz, Renzhi Han, Ting Si, Ronald Xu
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Pharmacology & Pharmacy
Poonam Parashar, Surabhi Pal, Monika Dwivedi, Shubhini A. Saraf
Article
Cell Biology
Lichao Wang, Binsheng Wang, Nathan S. Gasek, Yueying Zhou, Rachel L. Cohn, Dominique E. Martin, Wulin Zuo, William F. Flynn, Chun Guo, Evan R. Jellison, Taewan Kim, Larissa G. P. Langhi Prata, Allyson K. Palmer, Ming Li, Christina L. Inman, Lauren S. Barber, Iman M. A. Al-Naggar, Yanjiao Zhou, Wenqiang Du, Kshitiz, George A. Kuchel, Alexander Meves, Tamar Tchkonia, James L. Kirkland, Paul Robson, Ming Xu
Summary: This study identifies a small population of p21 high-expressing cells that accumulate in adipose tissue with obesity and shows that intermittent clearance of these cells can prevent and alleviate insulin resistance. Inactivation of the NF-kB pathway within these cells attenuates insulin resistance. Additionally, a drug combination of dasatinib and quercetin can eliminate p21 high-expressing cells in human fat and mitigate insulin resistance.
Article
Chemistry, Organic
Jyotsana Dwivedi, Monika Dwivedi, Swati Gupta, Vishwajeet Jadaun, Babita Yadav, Poonam Parashar, Priyanka Dhar, Pankaj Dwivedi, Hafsa Ahmed, Priyam Singh, Anirban Pal, Sarvesh Kumar Paliwal, Anil Kumar Dwivedi, Ajay Kumar Singh Rawat
Summary: Diabetes mellitus is a metabolic threat leading to various complications, and the synthesis of plant molecular hybrids from potent biomarkers has shown potential in combating diabetes, hyperlipidemia, and oxidative stress, with PMH4 standing out as particularly effective.
POLYCYCLIC AROMATIC COMPOUNDS
(2022)
Article
Cell Biology
Junaid Afzal, Yamin Liu, Wenqiang Du, Yasir Suhail, Pengyu Zong, Jianlin Feng, Visar Ajeti, Wasim A. Sayyad, Joerg Nikolaus, Maya Yankova, Alix C. Deymier, Lixia Yue, Kshitiz
Summary: Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have great potential in clinical models of cardiac pathologies, precision medicine, and drug screening. This study presents a method to accelerate the maturation of hiPSC-CMs on a cardiac mimetic matrix (CMM), resulting in rapid maturation within 30 days and exhibiting adult heart characteristics.
Article
Cell Biology
Junaid Afzal, Wenqiang Du, Ashkan Novin, Yamin Liu, Khadija Wali, Anarghya Murthy, Ashley Garen, Gunter Wagner, Kshitiz
Summary: There are similarities between placentation and cancer dissemination, particularly at the molecular, cellular, and anatomical levels. Placentation can be used as a unique model to understand the onset of cancer metastasis. The interaction between the placenta and the endometrium in humans leads to deep invasion of placental cells into the maternal tissue, similar to the invasion of carcinoma cells. The process of decidualization in the endometrium also involves changes in cellular contractility and energetic state, which are important for understanding implantation disorders and cancer dissemination.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Xiaoxiao Chen, Youjun Xia, Wenqiang Du, Han Liu, Ran Hou, Yiyu Song, Wenhu Xu, Yuxin Mao, Jianfeng Chen
Summary: Cancer metastasis is a leading cause of increased cancer incidence, recurrence, and mortality. This study investigates the contact guidance behavior of cancer cells in response to topographical constraints and determines the effects of substrate dimensions on cell morphology, density, and migration. Results show that increasing depth and decreasing groove width enhance cell elongation and alignment while inhibiting cell spreading. Cells preferentially distribute on the ridges, and their migration towards the ridges is driven by different cell-substrate interactions caused by geometrical constraints. The entropic force driven mechanism provides valuable insights for studying cell alignment and migration in healthy tissues of varying sizes, thus aiding in predicting cancer metastasis.
CELLULAR AND MOLECULAR BIOENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Sonia Kiran, Pankaj Dwivedi, Renuka Khatik, Sadaf Hameed, Monika Dwivedi, Fangsheng Huang, Ronald X. Xu
CHEMICAL COMMUNICATIONS
(2020)
Article
Materials Science, Biomaterials
Venkatesh Teja Banala, Sandeep Urandur, Shweta Sharma, Madhu Sharma, Ravi P. Shukla, Disha Marwaha, Shalini Gautam, Monika Dwivedi, Prabhat Ranjan Mishra
BIOMATERIALS SCIENCE
(2019)