Article
Biochemical Research Methods
Shalaka Konjalwar, Busenur Ceyhan, Oscar Rivera, Parisa Nategh, Mehrnoosh Neghabi, Mirjana Pavlovic, Shailaja Allani, Mahsa Ranji
Summary: Metformin hydrochloride and sulindac are FDA-approved drugs with anticancer effects. A combination of these drugs has been shown to enhance cell death in lung cancer cells through mitochondrial dysfunction and signaling pathway inhibition.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Endocrinology & Metabolism
Hibba Yousef, Ahsan H. H. Khandoker, Samuel F. F. Feng, Charlotte Helf, Herbert F. F. Jelinek
Summary: This study explores changes in inflammatory and oxidative stress biomarkers in plasma and urine, as well as mitochondrial oxidative stress biomarkers in patients with different stages of Type II diabetes mellitus (T2DM) in the presence or absence of hypertension (HT). The results highlight the importance of these biomarkers in discriminating between different stages of T2DM progression.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Ramana Vaka, Evangeline Deer, Babbette LaMarca
Summary: Despite extensive research, the pathogenesis of preeclampsia (PE) remains unclear and lacks FDA-approved treatments. Mitochondrial oxidative stress has been identified as a crucial factor in PE development and holds potential as a target for therapy.
Article
Biochemistry & Molecular Biology
Pauline Robert, Phuc Minh Chau Nguyen, Alexis Richard, Celine Grenier, Arnaud Chevrollier, Mathilde Munier, Linda Grimaud, Coralyne Proux, Tristan Champin, Eric Lelievre, Emmanuelle Sarzi, Emilie Vessieres, Samir Henni, Delphine Prunier, Pascal Reynier, Guys Lenaers, Celine Fassot, Daniel Henrion, Laurent Loufrani
Summary: The study found that mitochondrial fusion plays a protective role in hypertension by reducing oxidative stress. Defects in mitochondrial fusion worsen hypertension. Additionally, increased mitochondrial ROS production leads to apoptosis in vascular cells and decreased proliferation of VSMCs.
Article
Immunology
Leila dos Santos Moura, Vinicius Santana Nunes, Antoniel A. S. Gomes, Ana Caroline de Castro Nascimento Sousa, Marcos R. M. Fontes, Sergio Schenkman, Nilmar Silvio Moretti
Summary: The research highlights the involvement of lysine acetylation in the oxidative stress response of T. cruzi, especially through the mitochondrial lysine deacetylase TcSir2rp3 regulating the activity of mitochondrial superoxide dismutase A (TcSODA). The study also shows that acetylation of K97 in TcSODA plays a key role in modulating enzyme activity and maintaining redox homeostasis in trypanosomatids. Additionally, the interaction between TcSir2rp3 and TcSODA contributes to the understanding of mechanisms used by T. cruzi to progress during infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiao-Qing Li, Yi Wang, Shu-Jie Yang, Yu Liu, Xiang Ma, Lu Liu, Si-Hong Li, Dong Niu, Xing Duan
Summary: Maternal diabetes has negative effects on oocyte quality, but melatonin supplementation can protect oocytes from these damages and improve oocyte quality in assisted reproductive technologies.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Neurosciences
Yuta Kashiwagi, Hyun Yi, Shue Liu, Keiya Takahashi, Kentaro Hayashi, Daigo Ikegami, Xun Zhu, Jun Gu, Shuanglin Hao
Summary: Opioid use disorders (OUDs) have become epidemic in the United States, involving illicit use, prescription drugs, and counterfeit opioids. This study shows that spinal PGC-1 alpha can reduce morphine tolerance by decreasing mitochondria-targeted superoxide in the spinal cord dorsal horn.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Thomas Muenzel, Andreas Daiber
Summary: Cardiovascular disease and drug-induced health side effects are often associated with an imbalance between reactive oxygen and nitrogen species (RONS) and antioxidants. This review focuses on dysfunctional eNOS caused by RONS in the context of transportation noise exposure or chronic treatment with nitroglycerin (GTN). It also discusses various redox switches involved in the uncoupling process of eNOS.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Biochemistry & Molecular Biology
Dimitrios N. Zisimopoulos, Electra Kalaitzopoulou, Marianna Skipitari, Polyxeni Papadea, Nikolaos T. Panagopoulos, Georgios Salahas, Christos D. Georgiou
Summary: The study introduces a new method for quantification of 2-hydroxyethidium, showcasing innovations in efficient extraction and sensitive quantification protocols. This method can be applied to most biological systems for accurate detection.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Chemistry, Multidisciplinary
Namrata Singh, Somanathapura K. NaveenKumar, Motika Geethika, Govindasamy Mugesh
Summary: The study demonstrates that CeVO4 nanozyme can substitute the function of SOD1 and SOD2 in neuronal cells even when the natural enzymes are down-regulated. The nanozyme prevents mitochondrial damage by regulating superoxide levels and restores physiological levels of anti-apoptotic proteins. Additionally, it effectively prevents mitochondrial depolarization and improves cellular ATP levels under oxidative stress.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Joanna Majerczak, Agnieszka Kij, Hanna Drzymala-Celichowska, Kamil Kus, Janusz Karasinski, Zenon Nieckarz, Marcin Grandys, Jan Celichowski, Zbigniew Szkutnik, Ulrike B. Hendgen-Cotta, Jerzy A. Zoladz
Summary: The present study aimed to determine the basal levels of nitric oxide (NO) in skeletal muscles. The results showed that oxidative muscles with a higher content of mitochondrial electron transport chain proteins and myoglobin have lower nitrite concentration compared to fast-twitch muscles. Muscle type had no effect on nitrate concentration. Fast-twitch muscles have a greater potential to generate NO via nitrite reduction, which is important for supporting muscle blood flow and delaying muscle fatigue during strenuous exercise and hypoxia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Siarhei A. Dabravolski, Varvara A. Orekhova, Mirza S. Baig, Evgeny E. Bezsonov, Antonina Starodubova, Tatyana Popkova, Alexander N. Orekhov
Summary: Diabetes mellitus and related disorders are closely related to mitochondrial dysfunction. Specific types of mitochondrial diabetes, such as MIDD and DAD, have been identified. Mutations in mitochondrial DNA cause hereditary mitochondrial disorders, with tissue-specific phenotypes depending on the level of heteroplasmy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Sanela Kalinovic, Paul Stamm, Matthias Oelze, Sebastian Steven, Swenja Kroeller-Schon, Miroslava Kvandova, Jacek Zielonka, Thomas Muenzel, Andreas Daiber
Summary: This study used HPLC quantification to investigate superoxide formation in isolated leukocytes and tissues of hypertensive rats, finding a correlation between impaired functional parameters and increased superoxide formation in hypertensive rats. The impermeability of HPr+ allowed for differentiation between intra- and extracellular superoxide formation. Plate reader-based assays were found to have higher background signal and were inferior to HPLC-based methods in this study.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Cardiac & Cardiovascular Systems
Lesia Savchenko, Ilenia Martinelli, Dimitri Marsal, Vyacheslav Zhdan, Junwu Tao, Oksana Kunduzova
Summary: This study investigated the effects of prolonged electromagnetic stress on the mitochondrial oxidative phosphorylation system and myocardial structure. It was found that electromagnetic stress alters mitochondrial respiratory capacity but does not result in cardiac abnormalities.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Marion Mueller, Elfi Donhauser, Tibor Maske, Cornelius Bischof, Daniel Dumitrescu, Volker Rudolph, Anna Klinke
Summary: In order to develop targeted therapies for the growing patient population with right ventricular dysfunction (RVD) and right heart failure (RHF), molecular processes underlying these conditions need to be understood. Mitochondrial dysfunction has been identified as a potential key player in the development of RHF, affecting mitochondrial biogenesis, substrate utilization, redox balance, and oxidative phosphorylation. This review aims to comprehensively analyze the current knowledge on mitochondrial dysregulation in RVD and RHF and its relationship with the functional aspects of the right ventricle (RV).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Cardiac & Cardiovascular Systems
Thomas Muenzel, Tommaso Gori
CARDIOVASCULAR RESEARCH
(2023)
Review
Cardiac & Cardiovascular Systems
Lukas Hobohm, Ioannis T. Farmakis, Karsten Keller, Barbara Scibior, Anna C. Mavromanoli, Ingo Sagoschen, Thomas Muenzel, Ingo Ahrens, Stavros Konstantinides
Summary: This study systematically investigated the composition and clinical value of multidisciplinary pulmonary embolism response teams (PERTs). The results showed that PERT implementation led to greater use of advanced therapies and shorter in-hospital stay, but did not show a survival benefit in patients with PE.
CLINICAL RESEARCH IN CARDIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Thomas Muenzel, Andreas Daiber
Summary: Cardiovascular disease and drug-induced health side effects are often associated with an imbalance between reactive oxygen and nitrogen species (RONS) and antioxidants. This review focuses on dysfunctional eNOS caused by RONS in the context of transportation noise exposure or chronic treatment with nitroglycerin (GTN). It also discusses various redox switches involved in the uncoupling process of eNOS.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Psychiatry
Matthias Michal, Andreas Schulz, Philipp S. Wild, Thomas Koeck, Thomas Muenzel, Alexander K. Schuster, Konstantin Strauch, Karl Lackner, Sigurd D. Suessmuth, Heiko G. Niessen, Andreas Borta, Kelly A. Allers, Daniela Zahn, Manfred E. Beutel
Summary: Previous studies have shown altered tryptophan catabolite concentrations in major depression, suggesting their potential as biomarkers and treatment targets. However, these studies had small sample sizes, limiting their generalizability. In this population-based study with 3,389 participants, we found that clinically significant depression was associated with lower levels of kynurenine and kynurenic acid. However, the correlation between tryptophan catabolites and depression severity was very small, and they could not be used to diagnose depression.
Editorial Material
Cardiac & Cardiovascular Systems
Thomas Muenzel, Andreas Daiber, Omar Hahad
EUROPEAN JOURNAL OF PREVENTIVE CARDIOLOGY
(2023)
Article
Medicine, General & Internal
Karsten Keller, Volker H. Schmitt, Ingo Sagoschen, Thomas Muenzel, Christine Espinola-Klein, Lukas Hobohm
Summary: This study used the German nationwide inpatient sample and found that the CRB-65 score is helpful in identifying high-risk patients with PE and predicting adverse in-hospital events.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Berit Hackenberg, Julia Doege, Karoline O'Brien, Andrea Bohnert, Karl J. J. Lackner, Manfred E. E. Beutel, Matthias Michal, Thomas Muenzel, Philipp S. Wild, Norbert Pfeiffer, Andreas Schulz, Irene Schmidtmann, Christoph Matthias, Katharina Bahr
Summary: The aim of this study was to investigate the correlation between tinnitus, depression, anxiety, and somatic symptom disorders in a large population. The study found that participants with tinnitus had a higher prevalence of depression, anxiety, and somatic symptom disorders.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Cardiac & Cardiovascular Systems
Recha Blessing, Ioannis Drosos, Thomas Muenzel, Philip Wenzel, Tommaso Gori, Zisis Dimitriadis
Summary: This study aimed to evaluate the changes in right atrial function after revascularization of right coronary artery chronic total occlusion (CTO). Transthoracic echocardiography with strain imaging was used to assess right atrial function in 55 patients undergoing CTO recanalization. The results showed that successful revascularization improved right atrial function.
BMC CARDIOVASCULAR DISORDERS
(2023)
Editorial Material
Cardiac & Cardiovascular Systems
Thomas Muenzel, Omar Hahad, Andreas Daiber
EUROPEAN HEART JOURNAL
(2023)
Review
Cell Biology
Sanela Rajlic, Hendrik Treede, Thomas Muenzel, Andreas Daiber, Georg Daniel Duerr
Summary: Previous studies have shown that oxidative stress plays an important role in the development of cardiovascular disease (CVD) in diabetic patients with high blood sugar. CVD is still the leading cause of premature death in the western world. Therefore, it is crucial to recognize diabetes mellitus-associated oxidative stress and subsequent inflammation as early as possible to initiate appropriate treatment before the occurrence of cardiovascular complications such as hypertension or coronary artery disease (CAD). This review provides an overview of methods for quantifying reactive oxygen and nitrogen species (RONS) production, which originate from different cellular mechanisms in separate cellular compartments, in order to facilitate early detection and effective treatment of these patients and their cardiovascular sequelae.
Article
Public, Environmental & Occupational Health
Karsten Keller, Ioannis T. Farmakis, Luca Valerio, Sebastian Koelmel, Johannes Wild, Stefano Barco, Frank P. Schmidt, Christine Espinola-Klein, Stavros Konstantinides, Thomas Muenzel, Ingo Sagoschen, Lukas Hobohm
Summary: In Germany, 15.4% of hospitalized COVID-19 patients received ICU treatment in 2020. Male sex, cardiovascular diseases, and cardiovascular risk-factors were independent risk-factors for ICU admission.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Cardiac & Cardiovascular Systems
Hiroaki Yokoyama, Tobias Friedrich Ruf, Martin Geyer, Alexander R. R. Tamm, Jaqueline Grace Da Rocha E. Silva, Theresa Ann Maria Goessler, Julia Zirbs, Ben Schwidtal, Thomas Muenzel, Ralph Stephan von Bardeleben
Summary: In this study, the effect of COMBO-TMVr on the left cardiac chambers was evaluated, including survival rate and other clinical data. The results showed that the combination therapy can support reverse remodeling of left cardiac chambers in high-risk patients.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Review
Peripheral Vascular Disease
Omar Hahad, Sanjay Rajagopalan, Jos Lelieveld, Mette Sorensen, Marin Kuntic, Andreas Daiber, Mathias Basner, Mark Nieuwenhuijsen, Robert D. Brook, Thomas Muenzel
Summary: Traffic noise and air pollution are significant risk factors for cardiovascular events, with various mediating pathways involved. Existing evidence suggests that interventions targeting these environmental stressors can alleviate the adverse effects on blood pressure and intermediate pathways, providing support for a causal link. However, there are still gaps in our understanding of the underlying mechanisms, highlighting the need for further research.
Review
Peripheral Vascular Disease
Omar Hahad, Sanjay Rajagopalan, Jos Lelieveld, Mette Sorensen, Katie Frenis, Andreas Daiber, Mathias Basner, Mark Nieuwenhuijsen, Robert D. Brook, Thomas Muenzel
Summary: Traffic noise and air pollution are major environmental risk factors that often occur together in urban settings. Both noise and air pollution have been found to have independent effects on blood pressure, causing endothelial dysfunction, oxidative stress, vascular inflammation, circadian dysfunction, and activation of the autonomic nervous system, all of which contribute to the development of hypertension. It is important to increase the recognition of these environmental risk factors as modifiable cardiovascular risk factors in order to reduce the burden of cardiovascular disease.
Article
Biochemistry & Molecular Biology
Janina Krug, Magdalena L. Bochenek, Rajinikanth Gogiraju, Dagmar Laubert-Reh, Karl J. Lackner, Thomas Muenzel, Philipp S. Wild, Christine Espinola-Klein, Katrin Schaefer
Summary: This study found that circulating sEPCR levels may serve as biomarkers of endothelial dysfunction, including angiogenesis, in individuals older than 35 years, and progressive loss of endothelial protein C receptors might be involved in the development and progression of PAD.