Article
Pharmacology & Pharmacy
Seung Yeon Park, Wonjun Cho, A. M. Abd El-Aty, Ahmet Hacimuftuoglu, Ji Hoon Jeong, Tae Woo Jung
Summary: Valdecoxib (VAL) suppresses lipid accumulation in hepatocytes and alleviates hepatic steatosis through the AMPK/SIRT6 pathway. These findings suggest the potential of VAL as a therapeutic agent for non-alcoholic fatty liver disease.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Cell Biology
Wenxian Wu, Xiaojing Wang, Yadong Sun, Niklas Berleth, Jana Deitersen, David Schluetermann, Fabian Stuhldreier, Nora Wallot-Hieke, Maria Jose Mendiburo, Jan Cox, Christoph Peter, Ann Kathrin Bergmann, Bjoern Stork
Summary: This study identified RIPK3 as an AMPK-activating kinase, establishing a direct link between the kinases regulating autophagy and necroptosis.Activation of AMPK by RIPK3 leads to phosphorylation of autophagy-regulating proteins ULK1 and BECN1, while TNF-induced necroptosis blocks lysosomal degradation of autophagosomes. Dysregulation of SNARE complexes, including reduced levels of full-length STX17, was observed upon TNF treatment.
Article
Environmental Sciences
Yuanfeng Liu, Dongting Zhao, Wenyi Peng, Panpan Xue, Xiaojun Jiang, Shuting Chen, Huiqian Gao, Xinming Wang, Shaolong Feng
Summary: This study revealed that ambient PM2.5 exposure may block the autophagic flux in HUVECs by inhibiting the expression of Sntaxin-17 and LAMP2, indicating potential risks to cardiovascular health. Autophagic activity in HUVECs can serve as a useful biomarker for assessing the risks of environmental factors to human cardiovascular health.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Immunology
Shuai Gao, Dailin Yuan, Lingyu Gao, Fan Yang, Xu'ai Lin, Stijn van der Veen
Summary: Neisseria gonorrhoeae interacts tightly with mucosal epithelia through its type IV pilus, while pilus-negative gonococci residing in early and late endosomes are detected and targeted by nucleotide-binding oligomerization domain 1 (NOD1). NOD1 subsequently forms a complex with immunity-related guanosine triphosphatase M (IRGM) and autophagy-related 16-like 1 (ATG16L1) to activate autophagy and recruit microtubule-associated protein light chain 3 (LC3) to the intracellular bacteria. IRGM furthermore directly recruits syntaxin 17 (STX17), which is able to form tethering complexes with the lysosome. These findings demonstrate key roles for NOD1 and IRGM in the sensing of intracellular N gonorrhoeae and subsequent directing of the bacterium to the lysosome for degradation.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Editorial Material
Cell Biology
Baiying Li, Liwen Jiang
Summary: The regulation of autophagy in plants is still poorly understood, but recent research has identified the role of ufmylation components in maintaining ER homeostasis under salt stress. The expression of Ufm1-conjugating enzyme and Ufm1 protein increases in response to salt stress, indicating their involvement in regulating reticulophagy in plants.
Article
Biochemistry & Molecular Biology
Alessio Reggio, Viviana Buonomo, Rayene Berkane, Ramachandra M. Bhaskara, Mariana Tellechea, Ivana Peluso, Elena Polishchuk, Giorgia Di Lorenzo, Carmine Cirillo, Marianna Esposito, Adeela Hussain, Antje K. Huebner, Christian A. Hubner, Carmine Settembre, Gerhard Hummer, Paolo Grumati, Alexandra Stolz
Summary: The degradation of the endoplasmic reticulum via selective autophagy is crucial for cellular homeostasis. FAM134A and FAM134C have been identified as ER-phagy receptors that can induce ER fragmentation and lysosomal degradation. The FAM134 paralogues play a significant role in maintaining ER morphology and increasing ER-phagy flux.
Editorial Material
Cell Biology
Zhe Wu, Chuchu Zhou, Huilin Que, Yufen Wang, Yueguang Rong
Summary: During macroautophagy/autophagy, autophagosomes fuse with lysosomes to form autolysosomes. After fusion, the autophagosome inner membrane and enclosed substrates are degraded and transported out of lysosomes for recycling. The lysosomal membrane components are recycled by autophagic lysosome reformation (ALR) to generate new lysosomes.However, the fate of autophagosome outer membrane components on autolysosomes remains unknown. Our recent work discovered that autophagosome outer membrane components are not degraded but are recycled through an unidentified process which we named autophagosomal components recycling (ACR). Further investigation revealed the recycler complex (SNX4-SNX5-SNX17) responsible for ACR. The discovery of ACR not only fills a missing part in autophagy, but also reveals a new recycling pathway on autolysosomes.
Article
Plant Sciences
Tae Jin Kim, Do Hyeon Pyun, Myeong Jun Kim, Ji Hoon Jeong, A. M. Abd El-Aty, Tae Woo Jung
Summary: In this study, Compound K (CK) was found to prevent lipid-induced skeletal muscle apoptosis via AMPK/autophagy-mediated attenuation of ER stress and induction of myoblast differentiation. Therefore, CK may be suggested as a potential therapeutic approach for treating muscle-wasting conditions associated with obesity.
JOURNAL OF GINSENG RESEARCH
(2022)
Article
Cell & Tissue Engineering
T. J. Park, S. Y. Park, W. Cho, H. Oh, H. J. Lee, A. M. Abd El- Aty, C. Bayram, J. H. Jeong, T. W. Jung
Summary: This study reveals that DEL-1 can suppress inflammation and ER stress in tenocytes, thereby attenuating tenocyte apoptosis through AMPK/autophagy-mediated signaling.
BONE & JOINT RESEARCH
(2022)
Article
Oncology
Tianye Li, Zhenzhen Hao, Zihan Tang, Chunting Li, Linglin Cheng, Tao Wang, Xiaojin Zhu, Yunhao He, Yongye Huang, Bing Wang
Summary: BAP31 plays an important role in the migration of lung cancer cells, and its knockdown weakens cell migration. Treatment with TGF-beta can reduce cell migration and enhance proliferation, which is downregulated after BAP31 knockdown. Moreover, BAP31 regulates multiple signaling pathways, especially the Wnt signaling pathway.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Cheng-Yi Chang, Chih-Cheng Wu, Jiaan-Der Wang, Su-Lan Liao, Wen-Ying Chen, Yu-Hsiang Kuan, Wen-Yi Wang, Chun-Jung Chen
Summary: Elevation of intracellular cAMP levels through inhibition of phosphodiesterase using dipyridamole has shown to inhibit glioma cell proliferation, migration, and invasion. Dipyridamole induces apoptosis through Noxa-guided endoplasmic reticulum (ER) stress and impairs autophagic flux. YAP1 plays a role in the communication between ER stress and autophagy, promoting autophagy and protecting glioma cells from dipyridamole-induced apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Sung Woo Choi, Heeseung Oh, Seung Yeon Park, Wonjun Cho, A. M. Abd El-Aty, Nurcan Kilic Baygutalp, Ji Hoon Jeong, Tae Woo Jung
Summary: This study investigated the role of netrin-1 in attenuating hepatic steatosis by suppressing inflammation and endoplasmic reticulum (ER) stress through PPAR gamma/auto-phagy-dependent signaling. The study found that netrin-1 can promote autophagy and PPAR gamma expression, leading to a reduction in inflammation and ER stress, lipid accumulation, and lipogenic proteins in hepatocytes. Furthermore, administration of netrin-1 reversed the effects of a high-fat diet on hepatic ER stress and lipid deposition. These findings shed light on the pathogenesis of hepatic steatosis in obesity and suggest a potential therapeutic approach for metabolic-associated fatty liver disease (MAFLD).
Article
Cell Biology
Shaogui Wang, Xiaojuan Chao, Xiaoxiao Jiang, Tiantian Wang, Yssa Rodriguez, Ling Yang, Pal Pacher, Hong-Min Ni, Wen-Xing Ding
Summary: VMP1 plays a crucial role in the pathogenesis of pancreatitis by regulating autophagic degradation and ER stress, and its deficiency promotes inflammation and fibrosis. The study revealed the importance of VMP1 in modulating pancreatitis through the NFE2L2/Nrf2 pathway.
Article
Biochemistry & Molecular Biology
Esben M. Quistgaard
Summary: BAP31 is a broadly expressed transmembrane protein involved in various cellular processes such as supporting organelle homeostasis, promoting proliferation and migration, regulating metabolism and the immune system, and controlling autophagy and apoptosis. Loss-of-function mutations in BAP31 lead to DDCH syndrome and it is implicated in multiple cancers and diseases, targeted by several viruses for their survival.
Article
Multidisciplinary Sciences
Dale R. Balce, Ya-Ting Wang, Michael R. McAllaster, Bria F. Dunlap, Anthony Orvedahl, Barry L. Hykes, Lindsay Droit, Scott A. Handley, Craig B. Wilen, John G. Doench, Robert C. Orchard, Christina L. Stallings, Herbert W. Virgin
Summary: The study identified an important role of UFMylation in inhibiting responses to IFN-gamma and lipopolysaccharide, as deficiency in this system led to excessive activation of IFN-gamma and increased transcriptional responses. Additionally, UFMylation in myeloid cells is necessary for resistance to influenza infection in mice, indicating its modulation of in vivo infection responses.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)