4.7 Article

Effect of α-glycosidase inhibitors from endophytic fungus Alternaria destruens on survival and development of insect pest Spodoptera litura Fab. and fungal phytopathogens

Journal

SCIENTIFIC REPORTS
Volume 9, Issue -, Pages -

Publisher

NATURE PORTFOLIO
DOI: 10.1038/s41598-019-47838-6

Keywords

-

Funding

  1. Council of Scientific and Industrial Research (CSIR), New Delhi, India

Ask authors/readers for more resources

In the present study the production of alpha-glycosidase inhibitors was used as a strategy to screen endophytic fungi with insecticidal and antifungal potential. Endophytic fungi were isolated from Calotropis gigantea L. (Gentianales: Apocynaceae) and evaluated for their alpha-glycosidase inhibitory activity. Maximum inhibitory activity was observed in an isolate AKL-3, identified to be Alternaria destruens E.G.Simmons on the basis of morphological and molecular analysis. Production of inhibitory metabolites was carried out on malt extract and partially purified using column chromatography. Insecticidal potential was examined on Spodoptera litura Fab. (Lepidoptera: Noctudiae). Partially purified alpha-glycosidase inhibitors induced high mortality, delayed the development period as well as affected the adult emergence and induced adult deformities. Nutritional analysis revealed the toxic and antifeedant effect of AKL-3 inhibitors on various food utilization parameters of S. litura. They also inhibited the in vivo digestive enzymes activity in S. litura. Partially purified alpha-glycosidase inhibitors were also studied for their antifungal potential. Inhibitors demonstrated antifungal activity against the tested phytopathogens inducing severe morphological changes in mycelium and spores. This is the first report on production of alpha-glycosidase inhibitors from A. destruens with insecticidal and antifungal activity. The study also highlights the importance of endophytes in providing protection against insect pests and pathogens to the host.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Article Biotechnology & Applied Microbiology

An endophytic Schizophyllum commune Fr. exhibits in-vitro and in-vivo antidiabetic activity in streptozotocin induced diabetic rats

Avinash Sharma, Rajvir Kaur, Jasleen Kaur, Saweta Garg, Rajbir Bhatti, Amarjeet Kaur

Summary: The study isolated endophytic basidiomycetous fungi and evaluated their antidiabetic potential in-vitro and in-vivo. Results showed that ethyl acetate extract of S. commune exhibited significant antidiabetic effects in diabetic rats, improving blood glucose levels, renal parameters, lipid profile, antioxidant parameters, and liver enzymes. Phytochemical analysis revealed the presence of phenolics and terpenoids in the extract. This study highlights the therapeutic potential of endophytic S. commune in managing diabetes.

AMB EXPRESS (2021)

Article Multidisciplinary Sciences

Genome-wide characterization of peptidyl-prolyl cis-trans isomerases in Penicillium and their regulation by salt stress in a halotolerant P. oxalicum

Mangaljeet Singh, Kirandeep Kaur, Avinash Sharma, Rajvir Kaur, Dimple Joshi, Megha Chatterjee, Iman Dandapath, Amarjeet Kaur, Harpreet Singh, Prabhjeet Singh

Summary: Peptidyl-prolyl cis-trans isomerases (PPIases) are enzymes capable of cis-trans isomerization of the prolyl peptide bond, playing essential roles in various cellular processes. This study conducted a genome-wide analysis of PPIases in different Penicillium spp. and found variations in gene numbers and activities under salt stress. The halotolerant strain P. oxalicum showed significant enhancement in mycelial PPIase activity under salt stress, suggesting the importance of these enzymes in salt stress adaptation.

SCIENTIFIC REPORTS (2021)

No Data Available