Article
Nanoscience & Nanotechnology
Yongcong Fang, Mengke Ji, Bingyan Wu, Xinxin Xu, Ge Wang, Yanmei Zhang, Yingkai Xia, Zhe Li, Ting Zhang, Wei Sun, Zhuo Xiong
Summary: The study developed a GAP bioink for engineering highly vascularized bone tissues using prevascularized mesenchymal spheroids. The prevascularized spheroids formed an interconnected vascular network through angiogenic sprouting after printing. The results showed that prevascularized spheroids were more conducive to osteogenesis and angiogenesis.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Malin Becker, Melvin Gurian, Maik Schot, Jeroen Leijten
Summary: Embedded 3D bioprinting using low-viscosity ink 3D (LoV3D) bioprinting based on aqueous two-phase stabilization allows for the fabrication of living constructs at high speeds with high viability. The liquid/liquid interfaces of LoV3D bioprinting offer unique advantages for fusing structures, creating vasculature, and modifying surfaces. The low interfacial tension of LoV3D bioprinting allows for nozzle-independent control over filament diameter, enabling the printing of a wide range of diameters down to the width of a single cell.
Review
Chemistry, Multidisciplinary
Maurice N. Collins, Guang Ren, Kieran Young, S. Pina, Rui L. Reis, J. Miguel Oliveira
Summary: Bone tissue engineering aims to create biofunctional tissue for treating diseases, with scaffold fabrication techniques and biomaterial selection being crucial. Vascularization and immunomodulation are major challenges in this field.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Engineering, Biomedical
Yang Wu, Ming Li, Hao Su, Huaying Chen, Yonggang Zhu
Summary: This review discusses the major considerations, challenges, and potential strategies for bone bioprinting, and summarizes the latest progress in this field. It uncovers the immense potential of bioprinting in re-establishing the 3D dynamic microenvironment of the native bone, and can assist researchers in reconstructing clinically relevant bone tissues with appropriate mechanical properties and precisely regulated biological behaviors.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Review
Chemistry, Multidisciplinary
Alessandra Dellaquila, Chau Le Bao, Didier Letourneur, Teresa Simon-Yarza
Summary: The study discusses the importance of vascularization techniques in tissue engineering and the current research progress. By using different strategies and materials, more biologically relevant and organ-specific 3D models can be established, which are expected to be used for regenerative medicine and drug development in the future.
Article
Engineering, Biomedical
Yongcong Fang, Mengke Ji, Yi Yang, Yihan Guo, Ruobin Sun, Ting Zhang, Wei Sun, Zhuo Xiong
Summary: Three-dimensional bioprinting has been hindered by a lack of suitable bioinks for printing functional tissues. We developed a granular cell aggregate-based biphasic (GCAB) bioink that exhibited desired properties for extrusion bioprinting and modeling the mechanical characteristics of soft tissues. By preorganizing GCAB bioink and printing it with an endothelial cell-laden gelatin bioink, we successfully created functional hepatic tissues with high cell density and a perfusable vascular network. This GCAB bioink design has potential for creating functional tissues for therapeutic applications.
Review
Engineering, Biomedical
Yongcong Fang, Wei Sun, Ting Zhang, Zhuo Xiong
Summary: This review highlights the recent advances in bioengineering approaches for fabricating functional engineered cardiac pumps, focusing on methods for creating ECPs and the potential of 3D bioprinting techniques. It emphasizes the importance of high cell density, engineering vasculatures, and strategies for functional maturation of cardiac tissues.
Review
Biotechnology & Applied Microbiology
Yiting Ze, Yanxi Li, Linyang Huang, Yixin Shi, Peiran Li, Ping Gong, Jie Lin, Yang Yao
Summary: Mature vasculature is crucial for the survival of bioengineered tissue constructs. However, creating fully vascularized tissue constructs remains a great challenge in tissue engineering. Indirect 3D bioprinting has gained attention for its ability to create complex vascular network-like channels in thick tissue constructs while maintaining endothelial cell activity. Biodegradable materials play an important role in tissue engineering, especially in the selection of scaffolds and sacrificial materials in indirect 3D bioprinting.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yongcong Fang, Yihan Guo, Bingyan Wu, Zibo Liu, Min Ye, Yuanyuan Xu, Mengke Ji, Li Chen, Bingchuan Lu, Kaiji Nie, Zixuan Wang, Jianbin Luo, Ting Zhang, Wei Sun, Zhuo Xiong
Summary: A reversible ink template-based bioprinting strategy (SPIRIT) is developed to replicate the external geometry and internal structure of complex organs. This strategy utilizes a microgel-based bioink with shear-thinning and self-healing behavior to support embedded 3D printing, resulting in the generation of cardiac tissues and organoids. This technique allows for the rapid replication of complex organ geometry and internal structures, accelerating the biofabrication and therapeutic applications of tissue and organ constructs.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Ariel A. Szklanny, Majd Machour, Idan Redenski, Vaclav Chochola, Idit Goldfracht, Ben Kaplan, Mark Epshtein, Haneen Simaan Yameen, Uri Merdler, Adam Feinberg, Dror Seliktar, Netanel Korin, Josef Jaros, Shulamit Levenberg
Summary: Creating engineered hierarchical vasculatures is crucial for implantable functional thick tissues. This study introduces a combined approach using millimetric vessel-like scaffolds and 3D bioprinted vascularized tissues to achieve fully engineered hierarchical vascular constructs for implantation. The use of sacrificial molds, endothelial cells, and vascularizing hydrogels enables the formation of functional vessels within the engineered tissue, promoting tissue perfusion and host vasculature ingrowth.
ADVANCED MATERIALS
(2021)
Article
Materials Science, Multidisciplinary
Fei Xing, Jiawei Xu, Peiyun Yu, Yuxi Zhou, Man Zhe, Rong Luo, Ming Liu, Zhou Xiang, Xin Duan, Ulrike Ritz
Summary: Vascularization plays a crucial role in transporting and exchanging nutrients and oxygen between implanted grafts with the host tissue. In recent years, 3D bioprinting technologies have been widely used in fabricating various 3D grafts for tissue repair and regeneration. This study aimed to review recent advances, challenges, and future perspectives in biofabrication strategies based on bioprinting for vascularized tissue repair and regeneration, discussing techniques, bioinks, seed cells, and growth factors used, as well as bioprinting history, vessel formation mechanism, and histology of vascular networks in vascularized tissue.
MATERIALS & DESIGN
(2023)
Review
Biotechnology & Applied Microbiology
Astha Khanna, Beu P. Oropeza, Ngan F. Huang
Summary: A major challenge in engineering scalable three-dimensional tissues is the generation of a functional microvascular network. Current biological approaches involve vascular cells, soluble factors, and instructive biomaterials. This review highlights the developments in spatiotemporal control of these biological approaches through angiogenic factors, differentiation of vascular cells, and microfabrication of complex vascular networks.
BIOENGINEERING-BASEL
(2022)
Article
Chemistry, Analytical
Lothar Koch, Andrea Deiwick, Boris Chichkov
Summary: Bioprinting is a promising technique for tissue engineering, aiming to produce whole organs in the future. However, creating thick tissue with functional vascular networks, including capillaries, presents challenges, as current methods often result in randomly patterned small vessels.
Review
Cell Biology
Filip Simunovic, Guenter Finkenzeller
Summary: Bone is a highly vascularized tissue, and the development, maturation, remodeling, and regeneration of bone depend on tight regulation of blood vessel supply. In bone tissue engineering, various vascularization strategies are being utilized, such as angiogenic growth factors, co-implantation of blood vessel forming cells, ex vivo microfabrication of blood vessels through bioprinting, and surgical methods for creating composite tissues that can be surgically transferred.
Article
Engineering, Biomedical
Madison Jade Ainsworth, Nino Chirico, Mylene de Ruijter, Andrei Hrynevich, Inge Dokter, Joost P. G. Sluijter, Jos Malda, Alain van Mil, Miguel Castilho
Summary: In order to develop thick engineered cardiac tissue construct with a pre-vascularization pathway, a combination of extrusion-based bioprinting and melt electrowriting techniques was investigated. Gelatin methacryloyl (GelMA) was used as a base hydrogel and collagen, Matrigel, and fibrinogen were utilized as interpenetrating polymers to support myocardial functionality. An anatomically inspired vascular pathway was implemented in a dual extrusion-based bioprinting setup. The optimized bioinks effectively filled the microfiber mesh and maintained cell viability, and distinct cell populations were visualized in their respective patterns up to D14.
Article
Engineering, Biomedical
Zahra Khosrowpour, Seyed Mahmoud Hashemi, Samira Mohammadi-Yeganeh, Mehdi Moghtadaei, Peiman Brouki Milan, Lorenzo Moroni, Subhas C. Kundu, Mazaher Gholipourmalekabadi
Summary: The decellularized placental sponge shows potential as a bone substitute and provides a platform for communication between macrophages and mesenchymal stem cells, guiding the differentiation of stem cells towards bone cells.
Article
Biochemistry & Molecular Biology
Afonso Malheiro, Maria Thon, Ana Filipa Lourenco, Adrian Seijas Gamardo, Amit Chandrakar, Susan Gibbs, Paul Wieringa, Lorenzo Moroni
Summary: By deriving nociceptor neurospheres from induced pluripotent stem cells, a functional in vitro human skin model with a sensory neuron population is established. The model exhibits similar morphological and phenotypical characteristics to native skin, and the neurons innervate the artificial skin. The reaction of the neurons is analyzed by applying a clinically available capsaicin patch, showing the potential of this model for preclinical research.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Shahzad Hafeez, Ana A. Aldana, Hans Duimel, Floor A. A. Ruiter, Monize Caiado Decarli, Vanessa Lapointe, Clemens van Blitterswijk, Lorenzo Moroni, Matthew B. Baker
Summary: Traditional synthetic covalent hydrogels lack the native tissue dynamics and hierarchical fibrous structure found in the extracellular matrix (ECM). Here, the molecular tuning of a supramolecular benzene-1,3,5-tricarboxamide (BTA) hydrogelator via simple modulation of hydrophobic substituents is reported. These hydrogels show high cell viability for chondrocytes and human mesenchymal stem cells, establishing their use in tissue engineering applications.
ADVANCED MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Melissa Schepers, Afonso Malheiro, Adrian Seijas Gamardo, Niels Hellings, Jos Prickaerts, Lorenzo Moroni, Tim Vanmierlo, Paul Wieringa
Summary: Phosphodiesterase 4 (PDE4) inhibitors, such as roflumilast, have been extensively studied for their anti-inflammatory and neuroregenerative properties. This study found that roflumilast can promote differentiation of Schwann cells and accelerate myelination, as demonstrated in vitro using rat cells and a 3D co-culture model. These findings contribute to the development of PDE4 inhibition-based therapies in peripheral regenerative medicine.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Review
Engineering, Biomedical
Sophia Dalfino, Paolo Savadori, Marco Piazzoni, Stephen Thaddeus Connelly, Aldo Bruno Gianni, Massimo Del Fabbro, Gianluca Martino Tartaglia, Lorenzo Moroni
Summary: Mandibular tissue engineering aims to develop synthetic substitutes for critical size defects in the mandible, and scaffold design and fabrication using additive manufacturing techniques have become the focus of research efforts. This review discusses scaffolds made with additive manufacturing techniques in mandibular tissue engineering applications, including their chemical composition, structural properties, and strategies to promote osteogenesis and vascularization. In vivo studies comparing new bone formation, defect dimensions, and animal models are also reviewed.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Rong Wang, Febriyani Damanik, Tobias Kuhnt, Armand Jaminon, Shahzad Hafeez, Hong Liu, Hans Ippel, Pieter J. Dijkstra, Nicole Bouvy, Leon Schurgers, A. Tessa ten Cate, Aylvin Dias, Lorenzo Moroni, Matthew B. Baker
Summary: Digital Light Processing (DLP) is a precise and fast additive manufacturing technique used for producing various products. However, the lack of biodegradable resin development has hindered its use in tissue engineering. This study investigates a library of biodegradable poly(esters) capped with urethane acrylate as printable resins for tissue engineering. The synthesized oligomers show good printability and are capable of creating complex structures with mechanical moduli close to medium-soft tissues.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Medicine, Research & Experimental
Claudia Del Toro Runzer, Shivesh Anand, Carlos Mota, Lorenzo Moroni, Christian Plank, Martijn van Griensven, Elizabeth R. Balmayor
Summary: Nucleic acids have great potential for gene therapy, with plasmid DNA as the first therapeutic nucleic acid and mRNA as a safer and more affordable option. This study investigated the uptake mechanisms and efficiencies of genetic material by cells, focusing on different nucleic acids, delivery vectors, and human primary cells. The study found that caveolae-mediated uptake was the main route for gene delivery, pDNA had higher expression levels in fast-dividing fibroblasts, cmRNA was responsible for high protein production in slow-dividing osteoblasts, and in mesenchymal stem cells, the combination of vector and nucleic acid was more relevant. Protein expression was higher when cells were seeded on 3D scaffolds.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Chemistry, Multidisciplinary
Shahzad Hafeez, Monize Caiado Decarli, Agustina Aldana, Mahsa Ebrahimi, Floor A. A. Ruiter, Hans Duimel, Clemens van Blitterswijk, Louis M. Pitet, Lorenzo Moroni, Matthew B. Baker
Summary: This article investigates a synthetic supramolecular/covalent strategy for creating a tough hydrogel with the hierarchical fibrous architecture of the extracellular matrix (ECM). The hydrogelator self-assembles to form a viscoelastic hydrogel and is reinforced by covalent intra- and interfiber cross-links, resulting in enhanced mechanical properties. The hydrogels exhibit high tensile strain and compressive strain, are shear-thinning, and can be 3D bioprinted. Additionally, the hydrogels enable the bioprinting and differentiation of human mesenchymal stromal cell (hMSC) spheroids into chondrogenic tissue.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Applied
Mariana I. Neves, Mariana Magalhaes, Silvia J. Bidarra, Lorenzo Moroni, Cristina C. Barrias
Summary: This study explored the use of a biocompatible and bio-orthogonal click chemistry reaction to graft cyclooctyne-modified alginate with bi-azide-functionalized PVGLIG peptides, creating artificial extracellular matrices for tissue engineering applications.
CARBOHYDRATE POLYMERS
(2023)
Article
Engineering, Biomedical
Mahtab Asadian, Clarissa Tomasina, Yuliia Onyshchenko, Ke Vin Chan, Mohammad Norouzi, Jip Zonderland, Sandra Camarero-Espinosa, Rino Morent, Nathalie De Geyter, Lorenzo Moroni
Summary: This study investigates the effect of different surface properties induced by surface modifications on bone marrow-derived mesenchymal stromal cells (BMSCs) chondrogenic behavior. The results show that surface modifications can modulate the chondrogenic differentiation of BMSCs, and carboxylated nanofibers promote chondrogenesis.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Engineering, Biomedical
Zhengbo Wen, Yuxin Chen, Peilin Liao, Fengyu Wang, Weiping Zeng, Shoupei Liu, Haibing Wu, Ning Wang, Lorenzo Moroni, Minmin Zhang, Yuyou Duan, Honglin Chen
Summary: In this study, an in situ cell electrospinning system was developed as an attractive approach for stem cell delivery, which could promote cutaneous wound healing through direct deposition of bioactive fish gelatin fibers and MSCs onto wound sites, leading to a synergistic therapeutic effect.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Endocrinology & Metabolism
Marta Nazzari, Mirian Romitti, Duncan Hauser, Daniel J. Carvalho, Stefan Giselbrecht, Lorenzo Moroni, Sabine Costagliola, Florian Caiment
Summary: This study demonstrates that despite a limited number of differentially expressed genes, various analysis methods can be used to obtain relevant information on phthalates, highlighting the potential of in vitro thyroid-related systems for the analysis of endocrine disruptors.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Materials Science, Biomaterials
Antonio J. Feliciano, Eduardo Soares, Anton W. Bosman, Clemens van Blitterswijk, Lorenzo Moroni, Vanessa L. S. LaPointe, Matthew B. Baker
Summary: The ability to utilize directional supramolecular interactions to anchor an antifouling coating to a polymer surface containing a complementary supramolecular unit was investigated. By adjusting the UPy composition, the antifouling properties and lifespan of the coating were controlled. Surface modification employing supramolecular interactions provided an approach that combines the simplicity and scalability of nonspecific coating methodology with the specific anchoring capacity found when using conventional covalent grafting with longevity that could be engineered by the supramolecular composition itself.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Materials Science, Biomaterials
Julia Fernandez-Perez, Kenny A. A. van Kampen, Carlos Mota, Matthew Baker, Lorenzo Moroni
Summary: Coronary artery disease affects millions worldwide. Off-the-shelf grafts are needed for bypass surgeries, and this study describes the fabrication of tubular scaffolds using melt spinning. The scaffolds showed good performance in bending tests, no leakage when liquid was passed through, and successful suturing of native arteries. Mesenchymal stromal cells seeded on the scaffolds differentiated into vascular smooth muscle-like cells, and endothelial cells formed a tightly packed monolayer. These ECM-mimicking scaffolds have great potential for use as vascular conduits in vivo.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Gabriele Addario, Daphne Eussen, Sonja Djudjaj, Peter Boor, Lorenzo Moroni, Carlos Mota
Summary: Chronic kidney disease is the twelfth leading cause of death worldwide, with limited treatment options. A new in vitro organ-on-a-chip platform using 3D printing was developed to replicate segments of the nephron, the functional unit of the kidney. This low-cost manufacturing chip features perfusable channels and mimics the renal tubulointerstitium in vitro.
MACROMOLECULAR BIOSCIENCE
(2023)
