Article
Chemistry, Multidisciplinary
Malin Becker, Melvin Gurian, Maik Schot, Jeroen Leijten
Summary: Embedded 3D bioprinting using low-viscosity ink 3D (LoV3D) bioprinting based on aqueous two-phase stabilization allows for the fabrication of living constructs at high speeds with high viability. The liquid/liquid interfaces of LoV3D bioprinting offer unique advantages for fusing structures, creating vasculature, and modifying surfaces. The low interfacial tension of LoV3D bioprinting allows for nozzle-independent control over filament diameter, enabling the printing of a wide range of diameters down to the width of a single cell.
Article
Nanoscience & Nanotechnology
Yongcong Fang, Mengke Ji, Bingyan Wu, Xinxin Xu, Ge Wang, Yanmei Zhang, Yingkai Xia, Zhe Li, Ting Zhang, Wei Sun, Zhuo Xiong
Summary: The study developed a GAP bioink for engineering highly vascularized bone tissues using prevascularized mesenchymal spheroids. The prevascularized spheroids formed an interconnected vascular network through angiogenic sprouting after printing. The results showed that prevascularized spheroids were more conducive to osteogenesis and angiogenesis.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Chemistry, Multidisciplinary
Alessandra Dellaquila, Chau Le Bao, Didier Letourneur, Teresa Simon-Yarza
Summary: The study discusses the importance of vascularization techniques in tissue engineering and the current research progress. By using different strategies and materials, more biologically relevant and organ-specific 3D models can be established, which are expected to be used for regenerative medicine and drug development in the future.
Article
Engineering, Biomedical
Yongcong Fang, Mengke Ji, Yi Yang, Yihan Guo, Ruobin Sun, Ting Zhang, Wei Sun, Zhuo Xiong
Summary: Three-dimensional bioprinting has been hindered by a lack of suitable bioinks for printing functional tissues. We developed a granular cell aggregate-based biphasic (GCAB) bioink that exhibited desired properties for extrusion bioprinting and modeling the mechanical characteristics of soft tissues. By preorganizing GCAB bioink and printing it with an endothelial cell-laden gelatin bioink, we successfully created functional hepatic tissues with high cell density and a perfusable vascular network. This GCAB bioink design has potential for creating functional tissues for therapeutic applications.
Article
Chemistry, Multidisciplinary
Yongcong Fang, Yihan Guo, Bingyan Wu, Zibo Liu, Min Ye, Yuanyuan Xu, Mengke Ji, Li Chen, Bingchuan Lu, Kaiji Nie, Zixuan Wang, Jianbin Luo, Ting Zhang, Wei Sun, Zhuo Xiong
Summary: A reversible ink template-based bioprinting strategy (SPIRIT) is developed to replicate the external geometry and internal structure of complex organs. This strategy utilizes a microgel-based bioink with shear-thinning and self-healing behavior to support embedded 3D printing, resulting in the generation of cardiac tissues and organoids. This technique allows for the rapid replication of complex organ geometry and internal structures, accelerating the biofabrication and therapeutic applications of tissue and organ constructs.
ADVANCED MATERIALS
(2023)
Review
Medicine, Research & Experimental
Zihan Wang, Ling Wang, Ting Li, Sitian Liu, Baolin Guo, Wenhua Huang, Yaobin Wu
Summary: Heart disease is a leading cause of death globally, making it a significant challenge to rescue myocardial deficiency. Cardiac tissue engineering using 3D bioprinting offers a promising strategy for repairing heart defects, but there are still crucial challenges to overcome.
Article
Chemistry, Multidisciplinary
Ariel A. Szklanny, Majd Machour, Idan Redenski, Vaclav Chochola, Idit Goldfracht, Ben Kaplan, Mark Epshtein, Haneen Simaan Yameen, Uri Merdler, Adam Feinberg, Dror Seliktar, Netanel Korin, Josef Jaros, Shulamit Levenberg
Summary: Creating engineered hierarchical vasculatures is crucial for implantable functional thick tissues. This study introduces a combined approach using millimetric vessel-like scaffolds and 3D bioprinted vascularized tissues to achieve fully engineered hierarchical vascular constructs for implantation. The use of sacrificial molds, endothelial cells, and vascularizing hydrogels enables the formation of functional vessels within the engineered tissue, promoting tissue perfusion and host vasculature ingrowth.
ADVANCED MATERIALS
(2021)
Article
Engineering, Biomedical
Xin Liu, Xinhuan Wang, Liming Zhang, Lulu Sun, Heran Wang, Hao Zhao, Zhengtao Zhang, Wenli Liu, Yiming Huang, Shen Ji, Jingjinqiu Zhang, Kai Li, Biaobiao Song, Chun Li, Hui Zhang, Song Li, Shu Wang, Xiongfei Zheng, Qi Gu
Summary: This study presents a methodology for fabricating soft vascularized tissue at centimeter scale using multimaterial bioprinting with a customized multistage-temperature-control printer. The printed constructs can support 3D capillary networks to mimic mature and functional liver tissue, and also enable direct surgical anastomosis of blood vessels with the printed pressure-bearing layer. This versatile platform allows for optimal cellularization in engineered tissues through the replication of vasculature networks.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Materials Science, Multidisciplinary
Arman Jafari, Zineb Ajji, Ali Mousavi, Saman Naghieh, Sidi A. Bencherif, Houman Savoji
Summary: Cardiovascular diseases are a major cause of global mortality, and transplantation remains the gold standard for treatment. Tissue engineering and 3D bioprinting have shown promising results in cardiac regeneration. Researchers are exploring new strategies utilizing 3D bioprinting for cardiac tissue regeneration, with a focus on precision and complexity.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Review
Pharmacology & Pharmacy
Annika C. Dell, Grayson Wagner, Jason Own, John P. Geibel
Summary: 3D bioprinting plays a crucial role in tissue engineering in medicine by providing precise and highly customizable methods for creating biological tissues. The selection of appropriate cell ink formulations is vital for process optimization and customization to suit target tissues. Hydrogel-based cell ink formulations show promise for future development in bioprinting technology.
Review
Chemistry, Multidisciplinary
Maurice N. Collins, Guang Ren, Kieran Young, S. Pina, Rui L. Reis, J. Miguel Oliveira
Summary: Bone tissue engineering aims to create biofunctional tissue for treating diseases, with scaffold fabrication techniques and biomaterial selection being crucial. Vascularization and immunomodulation are major challenges in this field.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Engineering, Biomedical
Yang Wu, Ming Li, Hao Su, Huaying Chen, Yonggang Zhu
Summary: This review discusses the major considerations, challenges, and potential strategies for bone bioprinting, and summarizes the latest progress in this field. It uncovers the immense potential of bioprinting in re-establishing the 3D dynamic microenvironment of the native bone, and can assist researchers in reconstructing clinically relevant bone tissues with appropriate mechanical properties and precisely regulated biological behaviors.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Materials Science, Multidisciplinary
Yi Zhang, Piyush Kumar, Songwei Lv, Di Xiong, Hongbin Zhao, Zhiqiang Cai, Xiubo Zhao
Summary: 3D bioprinting technology combines computing science, biology, and material engineering, and has great potential in fabricating vascularized constructs for tissue engineering. This review summarizes recent advances in 3D bioprinting of vascularized tissues, including the selection of biomaterials and various 3D printing techniques and applications.
MATERIALS & DESIGN
(2021)
Review
Biotechnology & Applied Microbiology
Earnest P. Chen, Zeren Toksoy, Bruce A. Davis, John P. Geibel
Summary: The aim of tissue engineering with 3D bioprinting is to construct fully functional tissue and organ replacements for clinical applications. One of the main challenges is maintaining tissue viability through the inclusion of complex vascular networks. Recent advancements have made great strides in incorporating vascular networks in 3D printed tissue and organs, offering new possibilities for creating biologically functional tissues and organs.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Polymer Science
Yukun Xu, Dabin Song, Xiaohong Wang
Summary: Diabetes is a common chronic disease worldwide, posing a significant burden on health. Research on islet functionalization and 3D bioprinting is advancing rapidly, providing new possibilities for disease modeling and drug screening. Advanced 3D models of pancreatic islets have shown improved physiological functions compared to traditional cultures, suggesting potential breakthroughs in cell behavior research.
Article
Chemistry, Multidisciplinary
Yongcong Fang, Yihan Guo, Mengke Ji, Binhan Li, Yujiang Guo, Jieming Zhu, Ting Zhang, Zhuo Xiong
Summary: A generalizable strategy has been developed to formulate a cell-laden biphasic bioink for 3D extrusion bioprinting, which combines microgels with excellent rheological properties for printing and a hydrogel precursor for structural stability. This approach enables the printing of complex structures with high fidelity and offers great mechanical tunability and hyperelasticity, allowing for the encapsulation of different cell types for creating a heterogeneous cellular microenvironment at microscale. The MB bioink shows promise for various biomedical applications such as tissue engineering and soft robotics.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Review
Engineering, Biomedical
Xingwu Mo, Liliang Ouyang, Zhuo Xiong, Ting Zhang
Summary: This review summarizes the recent advances and applications of hydrogels in digital light processing (DLP) 3D printing. Hydrogels, with soft elasticity and good biocompatibility, are difficult to process into solid forms due to their high water content and soft nature. DLP technology provides opportunities for hydrogel manufacturing with its high printing speed and resolution. The challenges and future trends in hydrogel DLP are also discussed.
BIOMEDICAL MATERIALS
(2022)
Article
Engineering, Biomedical
Bingchuan Lu, Min Ye, Jingjing Xia, Zhenrui Zhang, Zhuo Xiong, Ting Zhang
Summary: The formation of multiscale vascular networks is essential for the construction of large-scale biomimetic cardiac tissues/organs in vitro. Electrical stimulation is applied to promote the elongation and connection of endothelial cells in engineered cardiac tissue (ECT) and achieve vascularization. The results show that electrical stimulation significantly enhances the elongation, migration, and interconnection of endothelial cells in ECT and increases the expression of related genes. Electrical stimulation also enhances the secretion of signal factors between cardiomyocytes and endothelial cells and contributes to the contractility of cardiac tissue.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Yongcong Fang, Chengjin Wang, Zibo Liu, Jeonghoon Ko, Li Chen, Ting Zhang, Zhuo Xiong, Lei Zhang, Wei Sun
Summary: In this study, a conductive multiscale filled NGC (MF-NGC) is developed, which significantly enhances peripheral nerve regeneration. The printed MF-NGCs possess good permeability, mechanical stability, and electrical conductivity, promoting the elongation and growth of Schwann cells and neurite outgrowth. Animal studies demonstrate that the MF-NGCs promote neovascularization and M2 transition, leading to improved peripheral nerve regeneration. This study demonstrates the feasibility of using 3D-printed conductive MF-NGCs with hierarchically oriented fibers as functional conduits.
Article
Engineering, Biomedical
Yongcong Fang, Mengke Ji, Yi Yang, Yihan Guo, Ruobin Sun, Ting Zhang, Wei Sun, Zhuo Xiong
Summary: Three-dimensional bioprinting has been hindered by a lack of suitable bioinks for printing functional tissues. We developed a granular cell aggregate-based biphasic (GCAB) bioink that exhibited desired properties for extrusion bioprinting and modeling the mechanical characteristics of soft tissues. By preorganizing GCAB bioink and printing it with an endothelial cell-laden gelatin bioink, we successfully created functional hepatic tissues with high cell density and a perfusable vascular network. This GCAB bioink design has potential for creating functional tissues for therapeutic applications.
Article
Nanoscience & Nanotechnology
Yijie Liu, Xuexuan Li, Ben Pei, Lin Ge, Zhuo Xiong, Zhen Zhang
Summary: Scanning probe lithography is a promising technology for nanoscale fabrication. This study proposes a novel framework for optimizing process parameters and segmenting features using machine learning. By extracting reliable information for statistical analysis, the framework enables the optimization of process parameters for smaller critical dimensions and large-scale nano-lithography.
MICROSYSTEMS & NANOENGINEERING
(2023)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.