Article
Genetics & Heredity
Kunhua Qin, Peng Huang, Ruopeng Feng, Cheryl A. Keller, Scott A. Peslak, Eugene Khandros, Megan S. Saari, Xianjiang Lan, Thiyagaraj Mayuranathan, Phillip A. Doerfler, Osheiza Abdulmalik, Belinda Giardine, Stella T. Chou, Junwei Shi, Ross C. Hardison, Mitchell J. Weiss, Gerd A. Blobel
Summary: NFIA and NFIX, members of the NFI transcription factor family, repress the expression of fetal-type beta-globin-like genes HBG1 and HBG2 in adult erythroid cells. This repression is achieved through the stimulation of BCL11A, a known silencer of HBG1/2 genes, and direct repression of HBG1/2 genes by NFIA and NFIX.
Article
Toxicology
Chun-Hong Yu, Shui-Qing Yang, Yu-Jing Zhang, Long Rong, Zong-Chun Yi
Summary: This study investigated the role of GATA factors in hydroquinone (HQ) inhibited erythroid differentiation in K562 cells. The results showed that HQ reduced the occupancy of GATA1 and increased the expression of GATA2, thereby inhibiting erythroid differentiation.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Pharmacology & Pharmacy
Fizza Iftikhar, Muhammad Behroz Naeem Khan, Syed Ghulam Musharraf
Summary: This study identified three non-toxic monoterpenes with significant erythroid expansion ability in K562 cells, while four compounds showed high potency in inducing HbF at both transcriptional and protein levels in vitro. The findings suggest monoterpenes as potential therapeutic agents for beta-thalassemia and sickle cell anemia, pending further mechanistic elucidation.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Oncology
Caiyan Hu, Yupeng Yan, Chengbing Fu, Jin Ding, Tiantian Li, Shuqiong Wang, Liu Fang
Summary: The study found that GATA-1 expression increased under hypoxia, and miR-210-3p acts as a positive regulator of erythroid differentiation, being upregulated in a GATA-1-dependent manner. Experimental confirmation showed that miR-210-3p is a downstream target of GATA-1 mediated regulation of erythropoiesis.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Nickolai A. Tchurikov, Elena S. Klushevskaya, Ildar R. Alembekov, Antonina N. Kretova, Vladimir R. Chechetkin, Galina I. Kravatskaya, Yuri V. Kravatsky
Summary: The expression of rDNA gene clusters affects pluripotency, but the mechanisms behind it are unknown. These clusters interact with multiple genes controlling differentiation in both human and Drosophila cells, indicating their potential role in 3D chromosomal structure formation and gene expression regulation. However, it is unclear whether inter-chromosomal rDNA contacts change during differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Yu-Hsuan Fu, Da-Liang Ou, Yi-Ru Yang, Kuan-Wei Su, Chien-Yuan Chen, Hwei-Fan Tien, Zheng-Sheng Lai, Che-Kun James Shen, Hsiung-Fei Chien, Liang-In Lin
Summary: Cabozantinib promotes erythroid differentiation in leukemia cells through upregulating heme biosynthesis, hemoglobin production, and erythroid-associated pathways, while inhibiting cell proliferation by downregulating cell survival pathways.
CANCER GENE THERAPY
(2022)
Article
Biology
Tomasz M. Grzywa, Anna Sosnowska, Zuzanna Rydzynska, Michal Lazniewski, Dariusz Plewczynski, Klaudia Klicka, Milena Malecka-Gieldowska, Anna Rodziewicz-Lurzynska, Olga Ciepiela, Magdalena Justyniarska, Paulina Pomper, Marcin M. Grzybowski, Roman Blaszczyk, Michal Wegrzynowicz, Agnieszka Tomaszewska, Grzegorz Basak, Jakub Golab, Dominika Nowis
Summary: The study shows that early CD71+ erythroid cells expand in the spleen of anemic mice and humans, utilizing high levels of arginase 2 (ARG2) and reactive oxygen species (ROS) to suppress T-cell activation. These findings provide insight into the regulatory role of CECs in the immune response.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Cristina Zuccato, Lucia Carmela Cosenza, Matteo Zurlo, Ilaria Lampronti, Monica Borgatti, Chiara Scapoli, Roberto Gambari, Alessia Finotti
Summary: This study analyzed Cinchona alkaloids as natural HbF-inducing agents in human erythroid cells, and found that cinchonidine and quinidine are potent inducers of gamma-globin mRNA and HbF in erythroid progenitor cells isolated from beta-thalassemia patients. These compounds may be considered for the development of pre-clinical approaches for therapeutic protocols of beta-thalassemia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Rino Takeuchi, Masato Suzuki, Tomoyuki Yasukawa
Summary: The electrorotation rates of K562 cells were estimated using a novel device to identify differentiation status without labeling. The rates increased with cell differentiation due to higher cytoplasm conductivity from increased iron ion concentrations for hemoglobin production. This method offers a non-invasive way to estimate cell differentiation stages.
ANALYTICAL SCIENCES
(2021)
Article
Chemistry, Applied
Maedeh Alipour, Nahid Nasiri, Fariba Kazemi, Farahnaz Zare, Sedigheh Sharifzadeh
Summary: The combination of resveratrol and hydroxyurea was found to effectively increase the expression of the gamma-globin gene, reduce reactive oxygen species levels, and alleviate the adverse effects of hydroxyurea.
NATURAL PRODUCT RESEARCH
(2023)
Article
Medicine, Research & Experimental
Pinyaphat Khamphikham, Natee Jearawiriyapaisarn, Amornrat Tangprasittipap, Suradej Hongeng
Summary: The downregulation of KLF4 is associated with increased embryonic and fetal globin gene expression in human erythroid progenitor cells, potentially providing an alternative therapeutic strategy for alpha-globin substitution in severe alpha-thalassemia.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Cell & Tissue Engineering
Sudjit Luanpitpong, Xing Kang, Montira Janan, Kanjana Thumanu, Jingting Li, Pakpoom Kheolamai, Surapol Issaragrisil
Summary: This study demonstrated the regulatory role of the O-GlcNAc/BCL11A axis in erythroid differentiation, maturation, and globin production. O-GlcNAcylation promotes HSCs toward erythroid differentiation and enucleation, and affects globin production. BCL11A is identified as a key mediator of O-GlcNAc-driven erythroid differentiation and β- and α-globin production. Metabolic reprogramming also plays a role in this process, as indicated by the unique metabolic fingerprints observed upon OGA inhibition.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Hematology
Yi Cheng, Xuan Shang, Diyu Chen, Dejian Pang, Cunyou Zhao, Xiangmin Xu
Summary: KLF6 has been identified as a potential regulator of erythropoiesis, with miR-2355-5p affecting gamma-globin synthesis by suppressing KLF6 expression. This interaction may offer valuable insights for the clinical management of beta-thalassaemia patients.
BRITISH JOURNAL OF HAEMATOLOGY
(2021)
Article
Medicine, General & Internal
Woratree Kaewsakulthong, Thunwarat Suriyun, Sukanya Chumchuen, Usanarat Anurathapan, Suradej Hongeng, Suthat Fucharoen, Orapan Sripichai
Summary: The pathophysiological properties of erythroid cells derived from different types of thalassemia were investigated. It was found that thalassemia cells exhibited accelerated expansion and limited differentiation. The severity of erythroid maturation arrest varied among different types of thalassemia. The induction of HSP70 transcripts was robust in thalassemia erythroid cells. Increased cell death was observed in homozygous beta(0)-thalassemia erythroblasts and associated with specific gene expression regulation.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Yuanyuan Han, Xiaoyu Tan, Tingting Jin, Siqi Zhao, Li Hu, Wei Zhang, Ryo Kurita, Yukio Nakamura, Juan Liu, Di Li, Zhaojun Zhang, Xiangdong Fang, Shengwen Huang
Summary: This study explores the potential use of CRISPR/Cas9 technology to edit the BCL11A gene for the treatment of beta-hemoglobinopathies. Multiplex gene editing was found to increase the expression of gamma-globin without inhibiting erythroid differentiation. Furthermore, the editing efficiency exceeded 50% when optimized. The study suggests the accuracy and effectiveness of this approach.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Vahid Iranpur Mobarakeh, Mohammad Hossein Modarressi, Pooneh Rahimi, Azam Bolhassani, Ehsan Arefian, Fatemeh Atyabi, Rouhollah Vahabpour
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2019)
Article
Biochemistry & Molecular Biology
Minoo Safari-Arababadi, Mohammad Hossein Modarressi, Mohammad Kazemi Arababadi
GENETICS AND MOLECULAR BIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Leila Farhady Tooli, Mahdieh Shirzad, Mohammad Hossein Modarressi, Hanifeh Mirtavoos-Mahyari, Mohammad Ali Amoozegar, Sedigheh Hantoushzadeh, Elahe Motevaseli
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Ashkan Safavi, Amirhosein Kefayat, Elham Mahdevar, Fatemeh Ghahremani, Navid Nezafat, Mohammad Hossein Modarressi
Summary: The study demonstrated that prophylactic co-immunization with multiepitope DNA and peptide cancer vaccines can significantly enhance the immune response against breast cancer, leading to reduced tumor volume and increased survival time in tumor-bearing mice. Further experiments are needed to evaluate their efficacy from different angles.
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2021)
Article
Genetics & Heredity
Elahe Valipour, Vajihe Taghdiri Nooshabadi, Shadi Mahdipour, Sasan Shabani, Leila Farhady-Tooli, Sina Majidian, Zahra Noroozi, Kamran Mansouri, Elaheh Motevaseli, Mohammad Hossein Modarressi
Article
Biochemistry & Molecular Biology
Roshanak S. Sajjadi, Mohammad Hossein Modarressi, Fahimeh Akbarian, Mohammad Amin Tabatabaiefar
Summary: This study utilized computational models to investigate the role of long noncoding RNAs in prostate cancer, identifying six high-value lncRNA targets through validation experiments. It revealed the relationship between these lncRNAs and coding genes associated with prostate cancer.
GENETIC TESTING AND MOLECULAR BIOMARKERS
(2021)
Article
Multidisciplinary Sciences
Zahra Noroozi, Mehdi Shamsara, Elahe Valipour, Sahar Esfandyari, Alireza Ehghaghi, Amir Monfaredan, Zahra Azizi, Elahe Motevaseli, Mohammad Hossein Modarressi
Summary: This study demonstrated that disrupting the HPV-E6 gene led to increased cell apoptosis and decreased cell proliferation. The antiproliferative effects of targeting HPV-E6 gene may provide therapeutic benefits for cervical cancer treatment.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Pouya Salehipour, Mojdeh Mahdiannasser, Ghazal Sedaghat Shayegan, Kimia Shankaie, Mina Tabrizi, Majid Mojarrad, Mohammad Hossein Modarressi
Summary: This study reports the development of a CRISPR-based fluorescent reporter (CBFR) assay for detecting the delE746_A750 subtype of EGFR exon 19 deletions. The CBFR assay offers a sensitive, specific, and simple strategy for detecting mutations, deletions, and pathogens in underequipped laboratories, and promoting personalized therapeutic approaches.
MOLECULAR BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Samaneh Ghanbari, Elham Bayat, Masoumeh Azizi, Pezhman Fard-Esfahani, Mohammad Hossein Modarressi, Fatemeh Davami
Summary: Recombinant CHO cell line development traditionally relies on random integration (RI) approach, but targeted integration offers potential advantages in terms of control and stability. In this study, we used the CRISPR-mediated precise integration into target chromosome (CRIS-PITCh) system combined with the Bxb1 recombinase-mediated cassette exchange (RMCE) system to generate a transgene-expressing cell line with improved specificity and stability.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mozhgan Mondeali, Ali Etemadi, Khabat Barkhordari, Mina Mobini Kesheh, Sara Shavandi, Atefeh Bahavar, Fatemeh Hosseini Tabatabaie, Mohammad Mahmoudi Gomari, Mohammad H. Modarressi
Summary: Mutations in the spike protein of SARS-CoV-2, particularly the S477N mutation, can increase its binding affinity for ACE2 and lead to enhanced immune system evasion through new hydrogen and hydrophobic bonding. Computational analysis showed structural changes and increased stability in the spike protein due to this mutation.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Maryamsadat Shahidi, Omid Abazari, Parisa Dayati, Javad Zavar Reza, Mohammad Hossein Modarressi, Davood Tofighi, Bibi Fatemeh Haghiralsadat, Fatemeh Oroojalian
Summary: This study developed a nanoparticle-based delivery system consisting of selenium nanoparticles modified by chitosan and hyaluronic acid, which successfully delivered PLK1 siRNAs to bladder cancer cells. These findings suggest that hyaluronic acid-chitosan-selenium nanoparticles may provide a good vehicle for targeted gene therapy for bladder cancer.
Article
Genetics & Heredity
Seyyed Jafar Nosratabadi, Nasim Hayati Roodbari, Ali Afgar, Mohammad Hossein Modarressi, Alireza Farsinejad, Majid Fasihi Harandi
Article
Genetics & Heredity
Nasibeh Soltani, Fatemeh Yazarlou, Mohammad Mehdi Akhondi, Maryam Sobhani, Mohammad Hossein Modarressi, Soudeh Ghafouri-Fard
Article
Multidisciplinary Sciences
Paria Sadat Lavasani, Elahe Motevaseli, Nafiseh Sadat Sanikhani, Mohammad Hossein Modarressi
Article
Genetics & Heredity
Fatemeh Yazarlou, Mandana Afsharpad, Vahid Kholghi Oskooei, Leila Nekoohesh, Tamouchin Moharrami, Niusha Samadaian, Soudeh Ghafouri-Fard, Mohammad Hossein Modarressi