Review
Biotechnology & Applied Microbiology
Alonso Ornelas-Gonzalez, Mirna Gonzalez-Gonzalez, Marco Rito-Palomares
Summary: Stem cell-based therapies have emerged as an alternative for treating various diseases, with the use of microcarrier culture techniques showing promise for expanding stem cells. However, challenges in production and medical applications remain, highlighting the need for the development of new bioprocesses to simplify stem cell cultures.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Tingting Gao, Xiyuan Zhao, Jie Hao, Yao Tian, Huike Ma, Wenjing Liu, Bin An, Faguo Sun, Shasha Liu, Baojie Guo, Shuaishuai Niu, Zhongwen Li, Chenxin Wang, Yukai Wang, Guihai Feng, Liu Wang, Wei Li, Jun Wu, Meijin Guo, Qi Zhou, Qi Gu
Summary: In this study, a new microcarrier with hyaluronic acid (HA) as the core material was developed for scalable serum-free suspension culture of hESC-derived MSCs. The optimal microcarriers, with a coating collagen concentration of 100 μg/mL or concave-structured surface (cHAMCs), allowed for the expansion of IMRCs within six days with a high yield. The harvested cells showed high viability, immunomodulatory and regenerative therapeutic promise, as well as increased secretion of extracellular matrix (ECM).
MATERIALS TODAY BIO
(2023)
Article
Biochemistry & Molecular Biology
Mohammad Mousaei Ghasroldasht, Jin Seok, Hang-Soo Park, Farzana Begum Liakath Ali, Ayman Al-Hendy
Summary: Regenerative medicine is a promising therapeutic mode using stem cells for the treatment of challenging human diseases. Stem cells have pleotropic therapeutic potential through inflammation or apoptosis inhibition, cell recruitment, angiogenesis stimulation, and differentiation. This review provides an overview of the translation process from idea to clinical practice, including guidelines for clinical trial approval and a review of regenerative medicine clinical trials registered on the Clinicaltrials.gov website.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Medicine, Research & Experimental
Yingqian Zhu, Jianli Ge, Ce Huang, Hailiang Liu, Hua Jiang
Summary: Aging frailty is a complex geriatric syndrome characterized by disruption of physiological homeostasis and declining health status. Factors such as genome instability, DNA damage, and stem cell exhaustion contribute to the development of frailty with advancing age. Effective interventions are needed to improve the health status of frail patients.
Article
Biochemistry & Molecular Biology
Ling Chen, Zhongwen Li, Wumei Wei, Bin An, Yao Tian, Wenjing Liu, Shuaishuai Niu, Yukai Wang, Liu Wang, Wei Li, Jie Hao, Jun Wu
Summary: Acute liver failure (ALF) is a clinical syndrome with high mortality. Liver transplantation is the only curative treatment for ALF, thus there is an urgent need for innovative therapies. This study evaluated the use of immunity-and-matrix regulatory cells (IMRCs) derived from human embryonic stem cells in treating ALF and investigated the underlying mechanism. The results showed that IMRCs improved liver histopathological changes, reduced liver enzyme levels in serum, promoted cell renewal, and protected against CCl4-induced liver damage.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Cell & Tissue Engineering
Shangwu Chen, Yushi Sato, Yasuhiko Tada, Yuma Suzuki, Ryosuke Takahashi, Masahiro Okanojo, Katsuhiko Nakashima
Summary: The study demonstrated the efficiency of using a bead-to-bead cell transfer method and medium containing nucleosides to scale up the expansion of human mesenchymal stem cells (hMSCs), resulting in enhanced cell proliferation and maintenance of cell characteristics. This approach showed potential for larger-scale manufacturing of hMSCs for cell therapy applications.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Richard Jeske, Shaquille Lewis, Ang-Chen Tsai, Kevin Sanders, Chang Liu, Xuegang Yuan, Yan Li
Summary: This study demonstrates a three-fold expansion of hMSCs in a microcarrier-based suspension bioreactor over seven days, resulting in smaller cell size, higher levels of ROS and Sirtuin-3. The study also shows increased Prostaglandin E-2 secretion and reduced Indoleamine-pyrrole 2,3-dioxygenase secretion in hMSCs in the bioreactor compared to static culture, indicating potential physiological alterations impacted by the bioreactor microenvironment during scalable production.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Review
Cell & Tissue Engineering
Ria Margiana, Alexander Markov, Angelina O. Zekiy, Mohammed Ubaid Hamza, Khalid A. Al-Dabbagh, Sura Hasan Al-Zubaidi, Noora M. Hameed, Irshad Ahmad, R. Sivaraman, Hamzah H. Kzar, Moaed E. Al-Gazally, Yasser Fakri Mustafa, Homayoon Siahmansouri
Summary: MSCs have great potential for immunomodulation and tissue regeneration, and can be used for the treatment of various diseases. Recent advances in MSCs biology have facilitated their clinical application.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Medicine, Research & Experimental
Sichen Zhang, Dashuai Zhu, Zhenhua Li, Ke Huang, Shiqi Hu, Halle Lutz, Mengjie Xie, Xuan Mei, Junlang Li, Genevieve Neal-Perry, Shaowei Wang, Ke Cheng
Summary: The therapeutic ovarian regenerative patch (ORP) has been found to effectively treat POI and restore fertility in rats by stimulating the proliferation of ovarian somatic cells and inhibiting apoptosis under injury stress. This cell-free, off-the-shelf, and clinically feasible treatment represents a promising approach for POI.
Article
Biochemistry & Molecular Biology
Thang Quoc Bui, Nguyen Trong Binh, Truc Le-Buu Pham, Trinh Le Van, Nhung Hai Truong, Dang Phu-Hai Nguyen, Thao Thi-Thu Luu, Trang Nguyen-Xuan Pham, Tu Cam Tran, Huyen Thuong-Thi Nguyen, Nhu Thuy-Trinh, Phong Anh Tran
Summary: In this study, the transplantation of human umbilical cord-derived mesenchymal stem cell (MSC) sheets was investigated as a potential treatment for myocardial infarction (MI). The findings showed that MSC sheets with higher cell density had better therapeutic effects on MI mice, with significant improvements in weight gain and ejection fraction recovery. In conclusion, hUC-MSC sheets ameliorated heart MI injury in mice and their efficacy improved with increased cell density.
Review
Cell Biology
Soudeh Ghafouri-Fard, Vahid Niazi, Bashdar Mahmud Hussen, Mir Davood Omrani, Mohammad Taheri, Abbas Basiri
Summary: Extracellular vesicles play important roles in cell-cell communication, inflammation modulation, cell proliferation, and tissue regeneration, with advantages such as lower risk and easier production and storage compared to cell therapy. Particularly, exosomes derived from mesenchymal stem cells are introduced as a therapeutic option for various human diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Medicinal
Aleksandra Skubis-Sikora, Bartosz Sikora, Weronika Malysiak, Patrycja Wieczorek, Piotr Czekaj
Summary: Melatonin has been evaluated for its safety and biological activity on ADSCs in vitro, and it was found that low-dose melatonin can improve cell viability without negatively affecting osteogenic potential.
Review
Agriculture, Dairy & Animal Science
Yanmin Wang, Michael Alexander, Todd Scott, Desiree C. T. Cox, Augusta Wellington, Mike K. S. Chan, Michelle B. F. Wong, Orn Adalsteinsson, Jonathan R. T. Lakey
Summary: Companion animals often suffer from ailments that cannot be treated by conventional veterinary medicine. Stem cell therapy, with its regenerative potential, offers a new approach for treating these ailments by promoting local tissue regeneration and repair. This review aims to summarize the various uses of stem cells in treating age-related and joint diseases in companion animals.
Review
Orthopedics
Zetao Wang, Youguo Liao, Canlong Wang, Chenqi Tang, Cailian Fang, Junchao Luo, Hengzhi Liu, Xianan Mo, Zicheng Wang, Lingfang Shen, Junjuan Wang, Xiao Chen, Zi Yin, Jianyou Li, Weiliang Shen
Summary: Rotator cuff tendinopathy is a common disorder that causes a significant burden. Although stem cell therapy has shown promising results, there are many unresolved issues, such as determining the optimal source and dosage of stem cells, as well as standardizing patient types.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2023)
Article
Cell & Tissue Engineering
Lydia J. Beeken, Darren S. J. Ting, Laura E. Sidney
Summary: Ocular surface inflammatory disorders are highly prevalent, heterogeneous diseases with a common factor of corneal inflammation. Current medications have limitations, and regenerative medicine cell therapies, particularly mesenchymal stem cells, show promise but require further investigation for successful clinical translation.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Review
Biotechnology & Applied Microbiology
Williams Olughu, Gurjot Deepika, Chris Hewitt, Chris Rielly
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Thomas R. J. Heathman, Alvin W. Nienow, Qasim A. Rafiq, Karen Coopman, Bo Kara, Christopher J. Hewitt
BIOCHEMICAL ENGINEERING JOURNAL
(2019)
Article
Biotechnology & Applied Microbiology
Elena Costariol, Marco Rotondi, Arman Amini, Christopher J. Hewitt, Alvin W. Nienow, Thomas R. J. Heathman, Martina Micheletti, Qasim A. Rafiq
BIOTECHNOLOGY AND BIOENGINEERING
(2019)
Article
Biotechnology & Applied Microbiology
Williams Olughu, Alvin Nienow, Chris Hewitt, Chris Rielly
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2020)
Article
Engineering, Multidisciplinary
Rebecca Grant, Karen Coopman, Nicholas Medcalf, Sandro Silva-Gomes, Jonathan J. Campbell, Bo Kara, Julian Braybrook, Jon Petzing
Article
Biotechnology & Applied Microbiology
Mariana P. Hanga, Junaid Ali, Panagiota Moutsatsou, Fritz A. de la Raga, Christopher J. Hewitt, Alvin Nienow, Ivan Wall
BIOTECHNOLOGY AND BIOENGINEERING
(2020)
Article
Biochemical Research Methods
Elena Costariol, Marco C. Rotondi, Arman Amini, Christopher J. Hewitt, Alvin W. Nienow, Thomas R. J. Heathman, Qasim A. Rafiq
BIOTECHNOLOGY JOURNAL
(2020)
Review
Biotechnology & Applied Microbiology
P. Silva Couto, M. C. Rotondi, A. Bersenev, C. J. Hewitt, A. W. Nienow, F. Verter, Q. A. Rafiq
BIOTECHNOLOGY ADVANCES
(2020)
Article
Cell & Tissue Engineering
Soukaina Bahsoun, Karen Coopman, Elizabeth C. Akam
STEM CELL RESEARCH & THERAPY
(2020)
Article
Biotechnology & Applied Microbiology
Marco Rotondi, Ned Grace, John Betts, Neil Bargh, Elena Costariol, Barney Zoro, Christopher J. Hewitt, Alvin W. Nienow, Qasim A. Rafiq
Summary: The emergence of cell and gene therapies has sparked interest, but due to high manufacturing costs and long development times, there is a need for more efficient platforms. By improving the design of the stirred-tank bioreactor, suspension of microcarriers/beads for cell culture has been enhanced, showing promise for future applications in cell therapy and gene therapy.
BIOTECHNOLOGY LETTERS
(2021)
Article
Biotechnology & Applied Microbiology
Jelena Ochs, Mariana P. Hanga, Georgina Shaw, Niamh Duffy, Michael Kulik, Nokilaj Tissin, Daniel Reibert, Ferdinand Biermann, Panagiota Moutsatsou, Shibani Ratnayake, Alvin Nienow, Niels Koenig, Robert Schmitt, Qasim Rafiq, Christopher J. Hewitt, Frank Barry, J. Mary Murphy
Summary: Advanced therapeutic medicinal products (ATMPs) have created a demand for large volumes of high-quality GMP cells. This study presents a fully automated, robot-assisted platform for scalable production of adherent human stem cells. The platform allows for a closed process from bone marrow collection to cell isolation, expansion, and collection into cryovials for patient delivery.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Article
Biotechnology & Applied Microbiology
Pedro Silva Couto, Dale J. J. Stibbs, Marco C. C. Rotondi, Yasuhiro Takeuchi, Qasim A. A. Rafiq
Summary: This study demonstrates a scalable manufacturing process using a microcarrier-bioreactor system for the expansion of gene-modified human mesenchymal stromal cells (hMSCs). The transduced and expanded cells met the criteria established by the International Society for Cell and Gene Therapy (ISCT). This work provides a foundation for the scalable manufacturing of gene-modified hMSCs.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Medicine, Research & Experimental
Carme Ripoll Fiol, Marie-Laure Collignon, John Welsh, Qasim A. Rafiq
Summary: This study optimized and developed a scalable, chemically defined, animal component-free LVV production process using adherent human embryonic kidney 293T cells in a fixed-bed bioreactor. By changing the process conditions, such as reducing cell seeding density, delaying transient transfection, reducing plasmid DNA dosage, and adding compound, the LVV titer was improved by 26.9-fold. The results showed comparable LVV titers with traditional culture methods at different scales.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2023)
Article
Biotechnology & Applied Microbiology
Mariana P. Hanga, Alvin W. Nienow, Halina Murasiewicz, Andrzej W. Pacek, Christopher J. Hewitt, Karen Coopman
Summary: The study demonstrated the use of perfluorocarbon liquid microcarriers for expanding and recovering human mesenchymal stem/stromal cells, showing that cells can successfully attach, proliferate, and maintain quality on these liquid carriers.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)