4.7 Article

Adenine-based small molecule fluorescent probe for imaging mitochondrial nucleic acid

Publisher

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2019.117865

Keywords

DNA; RNA; Adenine; Fluorescence probe; Cell imaging; Mitochondria

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Funding

  1. National Natural Science Foundation of China [21977078]
  2. Natural Science Foundation of Jiangsu Province [BK20181429]
  3. Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions
  4. Project of Scientific and Technologic Infrastructure of Suzhou [SZS201708]

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A small molecule fluorescent probe (probe 1) based on adenine-coumarin derivative was designed and synthesized in this paper. Probe 1 exhibited a significant fluorescence-enhancing response to nucleic acids at 495 nm (for DNA) and 487 nm (for RNA). The fluorescence enhancement of probe 1 for DNA and RNA was 5.68 and 9.73 times respectively, the fluorescence quantum yield was changed from 2.5% to 11.7% and 22.5% accordingly. Meanwhile, an excellent linear relationship of fluorescence intensity at 495 nm or 487 nm versus the nucleic acid concentration (1 mu M for probe 1, 0-350 mu g/mL for DNA and 0-300 mu g/mL for RNA) was obtained. Co-staining and nucleic acid digestion experiments showed that probe 1 could selectively image nucleic acids in mitochondria and nucleoli in HeLa cells. (C) 2019 Published by Elsevier B.V.

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