Article
Biochemistry & Molecular Biology
M. Mohamed Mahroop Raja, N. Reehana, A. Asrar Ahamed, A. Fasila Begum
Summary: The study observed the gradual increase of multiple antibiotic resistances in gram negative organisms such as Klebsiella pneumoniae and Proteus vulgaris, which are major causes of infection worldwide. Among nearly a hundred urine samples, 16 samples were found to contain plasmids with resistance to various antibiotics. The research focused on determining the resistant plasmid patterns in multi drug resistant Klebsiella pneumoniae and Proteus vulgaris isolated from urinary tract infection sites in hospital patients, as well as detecting and characterizing antimicrobial metabolites derived from marine sediments that exhibit potent activity against multidrug resistant pathogens.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Food Science & Technology
Lichang Sui, Zhengkai Yi, Xingning Xiao, Jiele Ma, Haoqi Huang, Chenggang Xu, Wen Wang, Xiaoyun Qu
Summary: This study utilized 16S rRNA sequencing and diversity analysis to investigate microbial contamination in a swine slaughterhouse and found cross-contamination during the slaughter process. Trimming emerged as a critical control point for cross-contamination. The disinfection procedure was partially effective in reducing microbial contamination.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Genetics & Heredity
Shougang Wang, Xiaoyan Su, Han Cui, Meng Wang, Xiaoli Hu, Wei Ding, Weipeng Zhang
Summary: This study compared the ability of full-length 16S rRNA gene sequencing technique on the PacBio platform and partial 16S rRNA gene sequencing on the traditional Illumina platform to identify microbes in marine biofilms. The results showed that marine biofilms had higher microbial species richness compared to seawater communities, and PacBio sequencing detected more specific species unique to biofilms. The enrichment of specific species in biofilms, such as Vibrio, Arcobacter, Photobacterium, Pseudoalteromonas, and Thalassomonas, confirms previous understanding of species adapted to a surface-associated lifestyle.
Article
Microbiology
Fangchao Song, Jennifer V. Kuehl, Arjun Chandran, Adam P. Arkin
Summary: In this study, a direct PCR method was evaluated as a cost-effective and time-saving alternative to traditional DNA extraction methods for analyzing microbial communities. The direct PCR method showed comparable efficiency to the conventional DNeasy PowerSoil protocol, with higher efficiency for extracting gDNA from Gram-negative strains. This method is significantly less expensive and faster, making it a potential solution for high-throughput large-scale bacterial community analysis.
Article
Microbiology
Fanie Shedleur-Bourguignon, Tom Duchemin, William P. Theriault, Jessie Longpre, Alexandre Thibodeau, Mounia N. Hocine, Philippe Fravalo
Summary: A study found that microorganisms on fresh meat at a slaughterhouse can affect the safety and spoilage patterns of the meat during processing. Using 16s rRNA sequencing, researchers analyzed the microbiota on conveyor belts in the cutting room. Significant differences in alpha and beta diversity were found between visits and production lines, and specific bacterial genera were associated with each visit and production line.
Article
Biotechnology & Applied Microbiology
Anuradha J. Punchihewage-Don, Nur A. Hasan, Shah M. Rashed, Salina Parveen
Summary: Investigating the chicken microbiome is important for establishing control measures against pathogens to protect consumers. This study aimed to compare the efficiency of detecting human pathogens through 16S rRNA sequencing of organic and conventional chickens using whole carcass enrichment (WCE) and rinse (WCR) methods. The results showed that Proteobacteria and Firmicutes were the predominant microorganisms in both conventional and organic chickens, and the WCE method provided comprehensive information on the chicken microbiome.
JOURNAL OF FOOD PROTECTION
(2023)
Article
Microbiology
Alexander Meene, Laurin Gierse, Theresa Schwaiger, Claudia Karte, Charlotte Schroeder, Dirk Hoeper, Haitao Wang, Verena Gross, Christine Wuensche, Pierre Muecke, Bernd Kreikemeyer, Martin Beer, Doerte Becher, Thomas C. Mettenleiter, Katharina Riedel, Tim Urich
Summary: This study investigates the structure and function of the archaeome in the porcine intestinal tract during a non-lethal Influenza A virus infection. It reveals significant changes in the abundance and composition of archaea along the ileum and colon, dominated by Methanobrevibacter and Methanosphaera genera. The study also explores the major methanogenesis pathways of these archaea and identifies some effects of the H1N1 infection on the central metabolism of gut archaea.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Cheng-Xing Long, Jie-Qi Wu, Zhou-Jin Tan
Summary: Intestinal microbiota characteristics were compared between pigs infected with African swine fever and healthy pigs. The results showed significant differences in bacterial alpha diversity, phylum taxa, and genus taxa between the two groups. Treponema was identified as an important inducer for swine pathogenicity.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Environmental Sciences
M. Rasheeda Shameem, J. Mary Isabella Sonali, P. Senthil Kumar, Gayathri Rangasamy, K. Veena Gayathri, V. Parthasarathy
Summary: The study isolated five nitrogen-fixing bacteria from Tea and Groundnut rhizosphere soil, and identified RSKVG 02 as the most optimal strain. The conditions for RSKVG 02 were determined to be pH 7 at 30 degrees C, with 1% glucose and 0.05% ammonium sulfate as carbon and nitrogen sources. This bacterium exhibited plant growth-promoting traits and the potential to enhance soil fertility. Therefore, Rhizobium mayense could be a valuable biofertilizer for crop production, increasing yield, and improving soil fertility.
ENVIRONMENTAL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Sina Moghadam, Arash Jafarzadeh, Kiran Kumar Vadde, Akanksha Matta, Samer Dessouky, Jeffrey Hutchinson, Vikram Kapoor
Summary: This study examined and compared the soil bacterial communities in two stormwater detention basins in Texas, USA, and evaluated the impact of soil physiochemical properties on these communities. The results showed that the basins had distinct microbial communities, and soil particle size, phosphorus, and ammonia were significantly correlated with some dominant phyla. The study also predicted the relative abundance of nitrogen cycling genes, indicating shared functional traits within the microbial communities. These findings have implications for understanding the role of microbial communities in nitrogen cycling processes and developing sustainable stormwater management strategies.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Shoichiro Kameoka, Daisuke Motooka, Satoshi Watanabe, Ryuichi Kubo, Nicolas Jung, Yuki Midorikawa, Natsuko O. Shinozaki, Yu Sawai, Aya K. Takeda, Shota Nakamura
Summary: The study compared 192 Japanese volunteers' fecal 16S analysis data using modified V1-V2 and standard V3-V4 primers. Results showed that the bacterial composition derived from the V34 region might differ from the actual abundance, suggesting that the modified V12 primer set is more desirable for analyzing the Japanese gut microbiota.
Article
Endocrinology & Metabolism
Qing Wang, Qi Wang, Lanbo Zhao, Yadi Bin, Li Wang, Lei Wang, Kailu Zhang, Qiling Li
Summary: The blood microbiome of PCOS patients showed significantly lower alpha diversity, different beta diversity, and significant taxonomic variations compared to healthy controls.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Multidisciplinary Sciences
Tingting Song, Yingyue Shen, Qunli Jin, Weilin Feng, Lijun Fan, Guangtian Cao, Weiming Cai
Summary: The study found that different agricultural straws exhibited variations in degradation under the same compost conditions, with wheat straw, rice straw, and cotton straw inducing significantly higher mushroom yields than other straws. Genera such as Thermobispora, Thermopolyspora, and Vulgatibacter may play important roles in the degradation of different agricultural straws. Adjusting formulations and compost methods based on different agricultural straws could potentially lead to high-yield mushroom production in the future.
Article
Environmental Sciences
Ademir Sergio Ferreira Araujo, Arthur Prudencio de Araujo de Pereira, Jadson Emanuel Lopes Antunes, Louise Melo de Souza Oliveira, Wanderley Jose de Melo, Sandra Mara Barbosa Rocha, Marineide Rodrigues do Amorim, Fabio Fernando Araujo, Vania Maria Maciel Melo, Lucas William Mendes
Summary: The use of composting as a biological alternative to improve tannery sludge quality by microbial action has been proposed. This study assessed the responses of bacterial and archaeal communities during composting using 16S rRNA sequencing, revealing specific microbial community structures and dynamics at different stages of the composting process.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Environmental Sciences
Ke Zhao, Xianbo Jia, Junjie Lin, Jie Zhao, Chenqiang Lin, Jichen Chen
Summary: Poultry waste can be turned into high-quality organic fertilizers through composting. This study analyzed the effectiveness of microbial agents and manure compost as additives in the composting process of chicken litter with mushroom residue. The results showed that the microbial agent exhibited the highest composting temperature, while the manure compost had the longest high-temperature period. The addition of mature compost increased microbial species, extended the high-temperature period, and promoted the fermentation process in composting.
WASTE AND BIOMASS VALORIZATION
(2023)
Article
Environmental Sciences
Toshimi Nakajima, Mao Kuragano, Makoto Yamada, Ryo Sugimoto
Summary: This study compared the contribution of submarine groundwater discharge (SGD) to river nutrient budgets at nearshore and embayment scales, and found that SGD-derived nutrients become more important at larger spatial scales.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Fan Liu, Lei Zhang, Chongyang Zhang, Ziguang Chen, Jingguang Li
Summary: NO2 emissions from wall-mounted gas stoves used for household heating have become a significant source of indoor pollution in Chinese urban areas. The high indoor concentration of NO2 poses potential health risks to residents. It is urgently necessary to establish relevant regulations and implement emission reduction technologies to reduce NO2 emissions from wall-mounted gas stoves.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Letter
Environmental Sciences
Hans Peter H. Arp, Raoul Wolf, Sarah E. Hale, Sivani Baskaran, Juliane Gluege, Martin Scheringer, Xenia Trier, Ian T. Cousins, Harrie Timmer, Roberta Hofman-Caris, Anna Lennquist, Andre D. Bannink, Gerard J. Stroomberg, Rosa M. A. Sjerps, Rosa Montes, Rosario Rodil, Jose Benito Quintana, Daniel Zahn, Herve Gallard, Tobias Mohr, Ivo Schliebner, Michael Neumann
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Philomina Onyedikachi Peter, Binessi Edouard Ifon, Francois Nkinahamira, Kayode Hassan Lasisi, Jiangwei Li, Anyi Hu, Chang-Ping Yu
Summary: This study investigates the relationship between dissolved organic matter (DOM) and Rare Earth Elements (REEs) in sediments from Yundang Lagoon, China. The results show four distinct fluorescent components, with protein-like substances being the most prevalent. Additionally, the total fluorescence intensity and LREE concentrations exhibit a synchronized increase from Outer to Inner to Songbai Lake core sediments. The findings demonstrate a strong correlation between DOM content and pollution levels.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Surya Gupta, Pasquale Borrelli, Panos Panagos, Christine Alewell
Summary: The objective of this study is to incorporate soil hydraulic properties into the erodibility factor (K) of USLE-type models. By modifying and improving the existing equations for soil texture and permeability, the study successfully included information on saturated hydraulic conductivity (Ksat) into the calculation of K factor. Using the Random Forest machine learning algorithm, two independent K factor maps with different spatial resolutions were generated. The results show that the decrease in K factor values has a positive impact on the modeling of soil erosion rates.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Jesmin Akter, Wendy J. M. Smith, Yawen Liu, Ilho Kim, Stuart L. Simpson, Phong Thai, Asja Korajkic, Warish Ahmed
Summary: The choice of workflow in wastewater surveillance has a significant impact on SARS-CoV-2 concentrations, while having minimal effects on HF183 and no effect on HAdV 40/41 concentrations. Certain components in the workflow can be interchangeable, but factors such as buffer type, chloroform, and homogenization speed can affect the recovery of viruses and bacteria.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Yu Luo, Xueting Yang, Diwei Wang, Hongmei Xu, Hongai Zhang, Shasha Huang, Qiyuan Wang, Ningning Zhang, Junji Cao, Zhenxing Shen
Summary: Atmospheric PM2.5, which can generate reactive oxygen species (ROS), is associated with cardiorespiratory morbidity and mortality. The study found that both the mass concentration of PM2.5 and the DTT activity were higher during the heating season than during the nonheating season. Combustion sources were the primary contributors to DTT activity during the heating season, while secondary formation dominated during the nonheating season. The study also revealed that biomass burning had the highest inherent oxidation potential among all sources investigated.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Erin L. Murphy, Leah R. Gerber, Chelsea M. Rochman, Beth Polidoro
Summary: Plastic pollution has devastating consequences for marine organisms. This study uses a trait-based framework to develop a vulnerability index for marine mammals, seabirds, and sea turtles in Hawai'i. The index ranks 63 study species based on their vulnerability to macroplastic pollution, providing valuable information for species monitoring and management priorities.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Kenji Maurice, Amelia Bourceret, Sami Youssef, Stephane Boivin, Liam Laurent-Webb, Coraline Damasio, Hassan Boukcim, Marc-Andre Selosse, Marc Ducousso
Summary: Growing pressure from climate change and agricultural land use is destabilizing soil microbial community interactions. Little is known about microbial community resistance and adaptation to disturbances, hindering our understanding of recovery latency and implications for ecosystem functioning. This study found that anthropic disturbance and natural disturbance have different effects on the topology and stability of soil microbial networks.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Yunhao Li, Yali Feng, Haoran Li, Yisong Yao, Chenglong Xu, Jinrong Ju, Ruiyu Ma, Haoyu Wang, Shiwei Jiang
Summary: Deep-sea mining poses a serious threat to marine ecosystems and human health by disturbing sediment and transmitting metal ions through the food chain. This study developed a new regenerative adsorption material, OMN@SA, which effectively removes metal ions. The adsorption mechanism and performance of the material for metal ion fixation were investigated.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Antonio Medici, Margherita Lavorgna, Marina Isidori, Chiara Russo, Elena Orlo, Giovanni Luongo, Giovanni Di Fabio, Armando Zarrelli
Summary: Valsartan, a widely used antihypertensive drug, has been detected in high concentrations in surface waters due to its unchanged excretion and incomplete degradation in wastewater treatment plants. This study investigated the degradation of valsartan and identified 14 degradation byproducts. The acute and chronic toxicity of these byproducts were evaluated in key organisms in the freshwater trophic chain.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Jiang Lin, Lianbao Chi, Qing Yuan, Busu Li, Mingbao Feng
Summary: This study investigated the photodegradation behavior and product formation of two representative pharmaceuticals in simulated estuary water. The study found that the formed transformation products of these pharmaceuticals have potential toxicity on marine organisms, including oxidative stress and damage to cellular components.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Hua Fang, Dongdong Jiang, Ye He, Siyi Wu, Yuehong Li, Ziqi Zhang, Haoting Chen, Zixin Zheng, Yan Sun, Wenxiang Wang
Summary: This study revealed that exposure to lower levels of air pollutants led to decreased pregnancy rates, with PM10, NO2, SO2, and CO emerging as the four most prominent pollutants. Individuals aged 35 and above exhibited heightened susceptibility to pollutants.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Ali Shaan Manzoor Ghumman, Rashid Shamsuddin, Amin Abbasi, Mohaira Ahmad, Yoshiaki Yoshida, Abdul Sami, Hamad Almohamadi
Summary: In this study, inverse vulcanized polysulfides (IVP) were synthesized by reacting molten sulfur with 4-vinyl benzyl chloride, and then functionalized using N-methyl D-glucamine (NMDG). The functionalized IVP showed a high mercury adsorption capacity and a machine learning model was developed to predict the amount of mercury removed. Furthermore, the functionalized IVP can be regenerated and reused, providing a sustainable and cost-effective adsorbent.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Rita Bonfiglio, Renata Sisto, Stefano Casciardi, Valeria Palumbo, Maria Paola Scioli, Erica Giacobbi, Francesca Servadei, Gerry Melino, Alessandro Mauriello, Manuel Scimeca
Summary: This study investigated the presence of aluminum in human colon cancer samples and its potential association with biological processes involved in cancer progression. Aluminum was found in tumor areas of 24% of patients and was associated with epithelial to mesenchymal transition (EMT) and cell death. Additional analyses revealed higher tumor mutational burden and mutations in genes related to EMT and apoptosis in aluminum-positive colon cancers. Understanding the molecular mechanisms of aluminum toxicity may improve strategies for the management of colon cancer patients.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)