Article
Biochemistry & Molecular Biology
Yin-Ying Wang, Hongen Kang, Tianyi Xu, Lili Hao, Yiming Bao, Peilin Jia
Summary: CeDR Atlas is a knowledgebase reporting computational inference of cellular drug response for various cell types, fine mapping drug response at cellular resolution and providing insights for the design of effective treatments. Through the use of high-throughput profiling of drug-induced gene expression and single-cell RNA sequencing data, it presents results for hundreds of cell types from different tissues and diseases, allowing exploration and search by keywords for drugs, cell types, tissues, diseases, and signature genes.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Chemistry, Analytical
Wenjie Zhao, Lingqian Zhang, Yifei Ye, Yuang Li, Xiaofeng Luan, Jinlong Liu, Jie Cheng, Yang Zhao, Mingxiao Li, Chengjun Huang
Summary: The study developed a method for isolating and detecting exosomes based on a microfluidic device, achieving successful isolation and quantitative detection of exosomes. By monitoring the protein expression levels in exosomes from different cell lines, the method shows promise for exosome-based tumor marker detection and cancer diagnosis.
Article
Biophysics
Jasmine Pramila Devadhasan, Alexander Jarrett Summers, Jian Gu, Stanley Smith, Baiju Thomas, Ali Fattahi, James Helton, Sujata G. Pandit, Marcellene Gates-Hollingsworth, Derrick Hau, Kathryn J. Pflughoeft, Douglas C. Montgomery, Supriya Atta, Tuan Vo-Dinh, David AuCoin, Frederic Zenhausern
Summary: This paper presents a simple, fast, and sensitive detection method for multiple biothreat agents using paper-based vertical flow colorimetric sandwich immunoassay. The optimized platform provides a portable and rapid on-site monitoring system for multiplex biothreat detection.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Nanoscience & Nanotechnology
Xiaofeng Luan, Pengbin Liu, Di Huang, Haiping Zhao, Yuang Li, Sheng Sun, Wenchang Zhang, Lingqian Zhang, Mingxiao Li, Tian Zhi, Yang Zhao, Chengjun Huang
Summary: Real-time transformation is crucial for impedance flow cytometry in practice, but the translation of raw data to cellular intrinsic electrical properties is time-consuming. We proposed a fast parallel physical fitting solver that characterizes single cells' electrical properties within 0.62 ms/cell. Based on this solver, we implemented physics-informed real-time impedance flow cytometry (piRT-IFC), which showed comparable speed but higher accuracy than the fully connected neural network predictor. In addition, we demonstrated the advantages of piRT-IFC in handling unfamiliar samples without pretraining.
MICROSYSTEMS & NANOENGINEERING
(2023)
Article
Chemistry, Analytical
Jizhong Wu, Wenya Wei, Muhammad Zareef, Shuhua Li, Qin Ouyang, Quansheng Chen
Summary: A nanoparticle-based aptasensor integrated with a microfluidic chip was designed to detect bisphenol A in food. The method has good linearity, low detection limit, and enables rapid determination of microsamples.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Physical
Lingjun Zhang, Jianfeng Sun, Yurui Fang, Yingzhou Huang, Hengyu Guo, Zhong Lin Wang
Summary: This study introduces an ultrasensitive SERS detection system using electrokinetic preconcentration with a triboelectric nanogenerator, which significantly enhances the efficiency of enriching target molecules on the substrate.
Article
Engineering, Electrical & Electronic
Yaoming Feng, Jingshun Pan, Dandan Sun, Shuixian Yang, Zhihao Fu, Lei Wan, Haiyan Shang, Donglei Shi, Shufen Zhu, Zhaohui Li
Summary: An on-chip self-referenced transducer assisted by digital optical frequency comb (DOFC) technology is proposed for detecting biomolecules at lower limits. This self-referenced structure eliminates temperature cross sensitivity and enables visualized spectral analysis and detection of subtle changes using high-resolution DOFC technology.
JOURNAL OF LIGHTWAVE TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Sangam Srikanth, U. S. Jayapiriya, Satish Kumar Dubey, Arshad Javed, Sanket Goel
Summary: A simple, cost-effective and miniaturized lab-on-a-chip platform has been developed for simultaneous cultivation and detection of bacteria, with high accuracy in quantifying bacterial concentration and validating viability, as well as real-time and on-field applications.
Article
Chemistry, Multidisciplinary
Joseph Broomfield, Melpomeni Kalofonou, Thomas Pataillot-Meakin, Sue M. Powell, Rayzel C. Fernandes, Nicolas Moser, Charlotte L. Bevan, Pantelis Georgiou
Summary: Prostate cancer is the second most common cause of male cancer-related death worldwide. Androgen deprivation therapy is the gold standard for treating advanced prostate cancer, but it often leads to castration-resistant prostate cancer. Detecting relevant biomarkers in the blood of castration-resistant patients could help personalize treatment options.
Article
Biotechnology & Applied Microbiology
Hongxia Li, Antony R. Warden, Wenqiong Su, Jie He, Xiao Zhi, Kan Wang, Laikuan Zhu, Guangxia Shen, Xianting Ding
Summary: The study proposes a novel two-step amplification method for sensitive detection of pancreatic cancer biomarkers, which combines target triggered Catalytic Hairpin Assembly strategy with Gold-Enhanced point-of-care-testing technology. This method successfully lowers the detection limit of mRNA to 100 fM and provides results within 2 hours.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Helen E. Parker, Sanghamitra Sengupta, Achar Harish, Ruben R. G. Soares, Haakan N. Joensson, Walter Margulis, Aman Russom, Fredrik Laurell
Summary: Microfluidics, specifically droplet microfluidics, is a rapidly developing field that offers independent manipulation and high-throughput analysis of droplets. In this study, we propose an optofluidic Lab-in-a-Fiber scheme using a periscope fiber for stable and compact alignment. We integrate droplet microfluidics with laser-induced fluorescence detection, demonstrating the generation of monodisperse droplets and achieving a limit of detection of fluorescein. Furthermore, we show the device's capability in detecting reverse-transcription loop-mediated isothermal amplification (RT-LAMP) products for COVID-19 diagnostics, highlighting its potential as a point-of-care droplet digital RT-LAMP platform.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Mai Pham, Quynh Pham, Ung Nguyen, Lanh Nguyen, Hoa Nguyen, Thang Vu, Ba Nguyen, Jakob Stenman, Ho Tho
Summary: The EGFR L858R missense mutation is more sensitive to EGFR tyrosine kinase inhibitor therapy, but there is still a significant non-response rate. A new one-step real-time RT-PCR assay based on RNA has been developed to detect the EGFR L858R mutation at the mRNA level. Compared to the DNA-based assay, this RNA-based assay can detect the EGFR L858R mutation in more FFPE samples and also shows a wide range of mRNA levels in tumor tissues. The circulating mRNA of the EGFR L858R mutation can be detected in the plasma of NSCLC patients.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Biophysics
Dhrubajyoti Das, Cheng-Wen Lin, Jae-Sung Kwon, Han-Sheng Chuang
Summary: In the wake of a pandemic, the development of rapid, simple, and accurate molecular diagnostic tests is crucial for reducing the spread of infections. This study combines particle imaging with molecular assays to create a biosensor that can quickly and sensitively detect the SARS-CoV-2 gene. The rotational diffusometry technique used in this study shows promising results for the rapid and sensitive detection of COVID-19.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Giorgio Ventimiglia, Massimiliano Pesaturo, Alastair Malcolm, Salvatore Petralia
Summary: A silicon lab-on-chip, integrating PCR and hybridization microarray, has been developed for nucleic acid detection. The lab-on-chip shows remarkable sensitivity, multiplexing analysis, and specificity for the detection of target sequences.
Article
Biophysics
M. Agostini, F. Amato, M. L. Vieri, G. Greco, I. Tonazzini, L. Baroncelli, M. Caleo, E. Vannini, M. Santi, G. Signore, M. Cecchini
Summary: GFAP has attracted attention as a promising biomarker for pathologies affecting the human central nervous system, with potential applications in clinical diagnostics for multiple brain-pathology biomarkers.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemistry & Molecular Biology
Monika Oberhuber, Matteo Pecoraro, Mate Rusz, Georg Oberhuber, Maritta Wieselberg, Peter Haslinger, Elisabeth Gurnhofer, Michaela Schlederer, Tanja Limberger, Sabine Lagger, Jan Pencik, Petra Kodajova, Sandra Hoegler, Georg Stockmaier, Sandra Grund-Groeschke, Fritz Aberger, Marco Bolis, Jean-Philippe Theurillat, Robert Wiebringhaus, Theresa Weiss, Andrea Haitel, Marc Brehme, Wolfgang Wadsak, Johannes Griss, Thomas Mohr, Alexandra Hofer, Anton Jaeger, Juergen Pollheimer, Gerda Egger, Gunda Koellensperger, Matthias Mann, Brigitte Hantusch, Lukas Kenner
MOLECULAR SYSTEMS BIOLOGY
(2020)
Article
Cell Biology
Tamara Scheidt, Oliver Alka, Humberto Gonczarowska-Jorge, Wolfgang Gruber, Florian Rathje, Margherita Dell'Aica, Marc Rurik, Oliver Kohlbacher, Rene P. Zahedi, Fritz Aberger, Christian G. Huber
CELL COMMUNICATION AND SIGNALING
(2020)
Article
Hematology
Julia C. Gutjahr, Elisabeth Bayer, Xiaobing Yu, Julia M. Laufer, Jan P. Hoepner, Suzana Tesanovic, Andrea Harzschel, Georg Auer, Tanja Riess, Astrid Salmhofer, Eva Szenes, Theresa Haslauer, Valerie Durand-Onayli, Andrea Ramspacher, Sandra P. Pennisi, Marc Artinger, Nadja Zaborsky, Alexandre Chigaev, Fritz Aberger, Daniel Neureiter, Lisa Pleyer, Daniel F. Legler, Veronique Orian-Rousseau, Richard Greil, Tanja N. Hartmann
Summary: The cooperation of CD44 and VLA-4 in AML was found to enhance leukemia cell adhesion on VCAM-1, leading to activation of Akt, MAPK, NF-kappa B and mTOR signaling pathways and decreased cell apoptosis. This mechanism provides insights into the regulation of AML cell retention in the bone marrow microenvironment.
Correction
Oncology
Nicole Prutsch, Elisabeth Gurnhofer, Tobias Suske, Huan Chang Liang, Michaela Schlederer, Simone Roos, Lawren C. Wu, Ingrid Simonitsch-Klupp, Andrea Alvarez-Hernandez, Christoph Kornauth, Dario A. Leone, Jasmin Svinka, Robert Eferl, Tanja Limberger, Astrid Aufinger, Nitesh Shirsath, Peter Wolf, Thomas Hielscher, Christina Sternberg, Fritz Aberger, Johannes Schmoellerl, Dagmar Stoiber, Birgit Strobl, Ulrich Jaeger, Philipp B. Staber, Florian Grebien, Richard Moriggl, Mathias Mueller, Giorgio G. Inghirami, Takaomi Sanda, A. Thomas Look, Suzanne D. Turner, Lukas Kenner, Olaf Merkel
Article
Oncology
Sandra Grund-Groeschke, Daniela Ortner, Antal B. Szenes-Nagy, Nadja Zaborsky, Richard Weiss, Daniel Neureiter, Martin Wipplinger, Angela Risch, Peter Hammerl, Richard Greil, Maria Sibilia, Iris K. Gratz, Patrizia Stoitzner, Fritz Aberger
MOLECULAR ONCOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Alexander Swoboda, Robert Soukup, Oliver Eckel, Katharina Kinslechner, Bettina Wingelhofer, David Schoerghofer, Christina Sternberg, Ha T. T. Pham, Maria Vallianou, Jaqueline Horvath, Dagmar Stoiber, Lukas Kenner, Lionel Larue, Valeria Poli, Friedrich Beermann, Takashi Yokota, Stefan Kubicek, Thomas Krausgruber, Andre F. Rendeiro, Christoph Bock, Rainer Zenz, Boris Kovacic, Fritz Aberger, Markus Hengstschlaeger, Peter Petzelbauer, Mario Mikula, Richard Moriggl
Summary: The study found that STAT3 can promote the metastatic process of melanoma and antagonize MITF through direct induction of CEBP family member transcription, thereby playing different roles in melanoma progression.
Article
Oncology
Sabrina Tripolt, Heidi A. Neubauer, Vanessa M. Knab, Dominik P. Elmer, Fritz Aberger, Richard Moriggl, Daniela A. Fux
Summary: Pain medication abuse poses risks for cancer progression, with opioid-induced breast cancer metastasis involving oncogenic JAK1/2-STAT3 signaling and promoting epithelial-mesenchymal transition. It highlights the importance of selective and restricted opioid use and the development of safer pain medication that does not activate oncogenic pathways.
Article
Biochemistry & Molecular Biology
Julia Bauer, Nicole Cuvelier, Nada Ragab, Katja Simon-Keller, Frauke Nitzki, Natalie Geyer, Dominik S. Botermann, Dominik P. Elmer, Albert Rosenberger, Thomas A. Rando, Stefano Biressi, James A. Fagin, Dieter Saur, Christian Dullin, Hans-Ulrich Schildhaus, Walter Schulz-Schaeffer, Fritz Aberger, Anja Uhmann, Heidi Hahn
Summary: It has been found that in ERMS, the Hedgehog (HH)/GLI signaling pathway and RAS signaling pathway interact with each other, with RAS mutations able to increase proliferation and tumorigenicity by inhibiting the HH signaling pathway. In a mouse model, different RAS mutations have different effects on ERMS development.
Article
Oncology
Elisabeth Peer, Sophie Karoline Aichberger, Filip Vilotic, Wolfgang Gruber, Thomas Parigger, Sandra Grund-Groeschke, Dominik Patrick Elmer, Florian Rathje, Andrea Ramspacher, Mirko Zaja, Susanne Michel, Svetlana Hamm, Fritz Aberger
Summary: Inhibition of CSNK1D represents a novel therapeutic approach for both SMO inhibitor-sensitive and -resistant tumors, suppressing oncogenic HH-GLI signaling and reducing malignant properties of tumor-initiating cells. This study provides a basis for the development of efficient CSNK1D inhibitors as a potential therapy for HH-GLI-associated cancers.
Article
Materials Science, Multidisciplinary
Pelin Toren, Andreas Proksch, Martin Smolka, Anja Haase, Dieter Nees, Stephan Ruttloff, Johannes Goetz, Christian Wolf, Bettina Hierschlager, Ingo Katzmayr, Max Sonnleitner, Barbara Stadlober, Jan Hesse
Summary: The Covid-19 pandemic highlighted the crucial need for accelerated, reliable and affordable virus testing systems, which rely on the mass production of disposable biochips for in vitro diagnostic testing. Utilizing roll-to-roll polymer structuring and UV nanoimprint lithography technologies can significantly increase the production efficiency and performance of biochips for IVD applications.
Review
Cell Biology
Suzana Tesanovic, Peter W. Krenn, Fritz Aberger
Summary: Despite advances in understanding the genetic alterations and biology of acute myeloid leukemia (AML), the translation into clinical treatment approaches is still in its early stages. A major challenge in AML therapy is the high relapse rates, largely due to the presence of treatment-resistant leukemic stem cells (LSCs). The Hedgehog (HH) signaling pathway has emerged as a therapeutic target in AML, as it plays a crucial role in the development and progression of cancer stem cell driven tumors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Oncology
Florian Rathje, Stefan Klingler, Fritz Aberger
Summary: Organoids derived from patient material serve as valuable preclinical models to mimic and study human diseased tissues, offering promising opportunities for personalized precision medicine and immuno-oncology drug development.
Article
Oncology
Laura Urwanisch, Michael Stefan Unger, Helene Sieberer, Hieu-Hoa Dang, Theresa Neuper, Christof Regl, Julia Vetter, Susanne Schaller, Stephan M. Winkler, Emanuela Kerschbamer, Christian X. Weichenberger, Peter W. Krenn, Michela Luciano, Lisa Pleyer, Richard Greil, Christian G. Huber, Fritz Aberger, Jutta Horejs-Hoeck
Summary: Epigenetic alterations play a significant role in cancer development, and the reversible nature of epigenetic changes makes them attractive targets for therapy. In this study, the authors investigated the expression of HDAC genes in AML patients and cell lines, and the effects of the HDAC inhibitor TMP269 on AML cells. They found significant overexpression of various HDACs in AML and observed that TMP269 treatment had effects on the proteome and growth of AML cells. Additionally, they demonstrated that the combination of TMP269 and venetoclax resulted in enhanced cell apoptosis. These findings highlight the potential of TMP269 as a therapeutic compound for AML.
Article
Biochemistry & Molecular Biology
Jan Pencik, Cecile Philippe, Michaela Schlederer, Emine Atas, Matteo Pecoraro, Sandra Grund-Groeschke, Wen (Jess) Li, Amanda Tracz, Isabel Heidegger, Sabine Lagger, Karolina Trachtova, Monika Oberhuber, Ellen Heitzer, Osman Aksoy, Heidi A. Neubauer, Bettina Wingelhofer, Anna Orlova, Nadine Witzeneder, Thomas Dillinger, Elisa Redl, Georg Greiner, David D'Andrea, Johnny R. Ostman, Simone Tangermann, Ivana Hermanova, Georg Schaefer, Felix Sternberg, Elena E. Pohl, Christina Sternberg, Adam Varady, Jaqueline Horvath, Dagmar Stoiber, Tim I. Malcolm, Suzanne D. Turner, Eileen E. Parkes, Brigitte Hantusch, Gerda Egger, Stefan Rose-John, Valeria Poli, Suneil Jain, Chris W. D. Armstrong, Gregor Hoermann, Vincent Goffin, Fritz Aberger, Richard Moriggl, Arkaitz Carracedo, Cathal McKinney, Richard D. Kennedy, Helmut Klocker, Michael R. Speicher, Dean G. Tang, Ali A. Moazzami, David M. Heery, Marcus Hacker, Lukas Kenner
Summary: PTEN and STAT3 are frequently co-deleted genes in metastatic prostate cancer (mPCa). STAT3 controls mPCa through the LKB1/pAMPK/mTORC1/CREB signaling pathway, and metformin can inhibit mPCa growth.
Article
Cell Biology
Sven Breunig, Veronika Wallner, Katharina Kobler, Herbert Wimmer, Peter Steinbacher, Maria Karolin Streubel, Johannes Bischof, Jutta Duschl, Claudia Neuhofer, Wolfgang Gruber, Fritz Aberger, Michael Breitenbach, Elisabeth Russel, Gottfried Wechselbergers, Albert Duranton, Klaus Richter, Mark Rinnerthaler
Summary: Calcium plays a crucial role in regulating the turnover of epidermal cells, with its gradient affecting cell proliferation and composition of the cornified envelope. The collapse of this gradient due to aging can have detrimental effects on the skin barrier.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)