Article
Multidisciplinary Sciences
Dilip Venugopal, Nanda Kasani, Yariswamy Manjunath, Guangfu Li, Jussuf T. Kaifi, Jae W. Kwon
Summary: A novel method using multifunctional hybrid functional microposts was developed to address the limitations of low flow rates and device clogging in processing rare tumor cells from blood as liquid biopsies. The method integrated a swift by-passing route for non-tumor cells to prevent clogging problems, leading to highly efficient separation of rare cells from biological fluids. Improved designs showed higher capture efficiencies for both microbeads and cancer cells.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
Kunpeng Cai, Shruti Mankar, Taiga Ajiri, Kentaro Shirai, Tasuku Yotoriyama
Summary: The study introduces a fully integrated high-throughput microfluidic circulatory fluorescence-activated cell sorting system capable of significantly increasing the purity of rare cells in a short period of time and effectively enriching different types of cells. Through a sequential sorting process and efficient contamination-free design, effective enrichment of clinical rare cells has been achieved.
Article
Multidisciplinary Sciences
Zhenglin Li, Chao Liu, Yangchang Cheng, Yike Li, Jinqi Deng, Lixiao Bai, Lili Qin, Huili Mei, Min Zeng, Fei Tian, Shaohua Zhang, Jiashu Sun
Summary: Tumor-derived extracellular vesicles (EVs) have the potential to improve noninvasive early cancer diagnosis. However, the lack of effective methods for isolating and detecting nanosized EVs in blood samples has been a challenge. To address this, researchers designed cascaded microfluidic circuits to filter EVs using a pneumatic clock pulse generator. This device allows for rapid and high-purity isolation of EVs from blood within 30 minutes and has shown 91% accuracy in diagnosing early-stage breast cancer.
Article
Multidisciplinary Sciences
Amir Shamloo, Amin Naghdloo, Mohsen Besanjideh
Summary: The study focused on isolating rare cancer cells using two centrifugal microfluidic devices. The passive design isolates cells through inertial effects and bifurcation law, while the hybrid design captures magnetically labeled cells using permanent magnets at the end of the microchannel. The hybrid design offers better separation efficiency and purity, while the passive one is simpler and cost-effective without the need for cell labeling.
SCIENTIFIC REPORTS
(2021)
Review
Chemistry, Multidisciplinary
Laura M. Rey Gomez, Rena Hirani, Andrew Care, David W. Inglis, Yuling Wang
Summary: Blood testing is crucial for diagnosis and monitoring of various conditions, and microfluidic devices offer a simpler and more affordable solution for blood sample preparation. However, currently there are limited microfluidic devices designed for undiluted whole blood, which can eliminate the need for blood dilution and minimize sample preparation.
Article
Biochemical Research Methods
Eliet H. Sipos, Adelaide Lety-Stefanska, Cyril Denby Wilkes, Julie Soutourina, Florent Malloggi
Summary: The study developed a microfluidic system that automates yeast cell cultivation for mutation accumulation, reducing the time required for measuring the entire genome mutational profile. By performing experiments in different genetic backgrounds, it was shown that the microfluidic device allows for mutation accumulation comparable to traditional methods.
Article
Chemistry, Analytical
Fengtao Jiang, Nan Xiang
Summary: This paper presents the development of a novel handheld sorter with a multifunction integrated microfluidic chip for point-of-care diagnosis in resource-limited settings. The sorter utilizes a spiral inertial microfluidic channel and flow regulators for size-based cell separation. The prototype of the sorter successfully separates malignant tumor cells from clinical pleural effusions. The device is simple to use, inexpensive, portable, and compact, making it suitable for high-throughput label-free cell separation in resource-limited areas.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Aliakbar Ebrahimi, Reza Didarian, Hamed Ghorbanpoor, Fatma Dogan Guzel, Hossein Hashempour, Huseyin Avci
Summary: This study used a microfluidic chip-based mimic of high-performance liquid chromatography (HPLC) strategy to successfully separate cyclotides. The results demonstrate that this method is a rapid, cost-effective, and simple way to separate cyclotides, with potential applications in drug discovery research.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Suryong Kim, Jihoon Ko, Seung-Ryeol Lee, Dohyun Park, Seunghyuk Park, Noo Li Jeon
Summary: In vitro models are advancing, with organ-on-a-chip devices providing significant results but requiring further development. Anchor-IMPACT is a 3D anchor-based microfluidic injection-molded plastic array culture platform that enables selective 3D cell patterning for high-throughput angiogenesis evaluation.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Chemistry, Analytical
Alan M. Gonzalez-Suarez, Gulnaz Stybayeva, William A. Carey, Alexander Revzin
Summary: We have developed a microfluidic device that enables rapid analysis of biomarkers in whole blood, providing high sensitivity and real-time monitoring. This device has applications in monitoring hypoglycemia and detecting other biomarkers in whole blood.
ANALYTICAL CHEMISTRY
(2022)
Review
Biotechnology & Applied Microbiology
Jinfeng Yan, Tong Wu, Jinjin Zhang, Yueyue Gao, Jia-Min Wu, Shixuan Wang
Summary: A comprehensive understanding of the female reproductive system is crucial for protecting fertility and preventing women's health issues. Microfluidic chips have revolutionized the knowledge and management of female reproductive health, ultimately promoting the development of assisted reproductive technologies, treatments, and drug screening approaches.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Kentaro Shirai, Guofeng Guan, Tan Meihui, Peng Xiaoling, Yuma Oka, Yusuke Takahashi, Ali Asgar S. Bhagat, Masatoshi Yanagida, Shigeki Iwanaga, Nobuaki Matsubara, Toru Mukohara, Tomokazu Yoshida
Summary: This study developed a novel cancer cell separation chip that can quickly process cancer cells from whole blood without the need for RBC lysis and centrifugation. The method achieves high separation precision and throughput, making it suitable for automated clinical analysis of rare CTCs.
Article
Biochemical Research Methods
Yang Bu, Jinhui Wang, Sheng Ni, Yusong Guo, Levent Yobas
Summary: Extracellular vesicles (EVs) are cell-derived bioparticles that have significant roles in various biological processes and can be potential biomarkers for pre-diagnostic applications. However, isolating EV subpopulations, especially exosomes, from biological fluids is challenging due to their small size. In this study, a microfluidic device combining electrothermal fluid rolls and dielectrophoresis was used for continuous-flow label-free size fractionation of EVs. The device showed high efficiency in separating submicron particles and isolating intact exosomes from cell culture medium or blood serum. This method provides a promising platform for the purification of target bioparticles directly from physiological fluids and may have important implications in EV-related diagnostic applications.
Article
Chemistry, Analytical
Abdurrahim Yilmaz, Zeynep Karavelioglu, Gizem Aydemir, Ali Anil Demircali, Rahmetullah Varol, Ali Kosar, Huseyin Uvet
Summary: This study presents a functional system utilizing an untethered magnetic microrobot to create wounds in a microfluidic platform without negative effects on cells. The study also investigates the effect of different wound geometries on wound healing, finding that triangle-shaped wounds heal the slowest and plus-shaped wounds heal the fastest. This research could pave the way for the use of microrobots in lab-on-a-chip devices and potential application in 3D cell cultures.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Materials Science, Multidisciplinary
Sanghyun Lee, Hojin Kim, Sung Yang
Summary: The separation of blood components is essential for biology, clinical diagnosis, therapeutics, and personalized medicine. Microfluidics has become a popular technology for this purpose, allowing for the separation of target cells based on their intrinsic properties. This review discusses various microfluidic-based hydrodynamic cell-separation technologies, categorizing them according to their working principles. The relative performances and features of these technologies are thoroughly discussed, along with future perspectives on commercialization and standardization for widespread use and applications.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Biochemical Research Methods
Liang Huang, Xu Zhang, Yongxiang Feng, Fei Liang, Wenhui Wang
Summary: Leveraging advances in microfluidics and imaging technology, a new paradigm of large-scale, high-content drug screening solutions for rapid biological processes, like cardiotoxicity, has been established. The designed microfluidic chips enable simultaneous assay of 10 types of drugs each with 5 concentrations, along with high-resolution imaging system for real-time video rate imaging. The HCS system demonstrated unparalleled capability in revealing spatiotemporal patterns of cardiomyocyte imaging and validating cardiotoxicity of certain molecules.
Article
Biochemical Research Methods
Yongxiang Feng, Zhen Cheng, Huichao Chai, Weihua He, Liang Huang, Wenhui Wang
Summary: The study demonstrates the use of neural network technology to achieve real-time characterization of cellular intrinsic properties and classification based on intrinsic parameters, significantly improving calculation speed. Experimental results show a high accuracy in cell type classification and minimal impact on cell properties from IFC testing.
Article
Multidisciplinary Sciences
Zijian Xu, Daoming Chen, Yucheng Hu, Kaiju Jiang, Huanwei Huang, Yingxue Du, Wenbo Wu, Jiawen Wang, Jianhua Sui, Wenhui Wang, Long Zhang, Shuli Li, Chunying Li, Yong Yang, Jianmin Chang, Ting Chen
Summary: The skin serves as a physical barrier and immunological interface that protects the body from external environment. Aberrant activation of immune cells can lead to autoimmune skin diseases like vitiligo, characterized by symmetric lesions. Understanding the role of dermal fibroblast subsets in orchestrating immune activities at an organ level is crucial for autoimmune disease treatment.
Article
Nanoscience & Nanotechnology
Yujia Zhang, Minjian Lu, Tao Wu, Kun Chen, Yongxiang Feng, Wenhui Wang, Yan Li, Haoyun Wei
Summary: A novel delay-spectral focusing dual-comb CARS scheme is proposed in this study for rapid HW Raman detection. By combining the advantages of DC asynchronous optical sampling and spectral focusing, the Raman spectrum is directly mapped to DC relative delay, releasing the coherence constraint of excitation sources. Rapid acquisition rate tuning and high-throughput measurement in the HW region are achieved with stable spectral resolution and signal-to-noise ratio.
Article
Chemistry, Physical
Yongxiang Feng, Huichao Chai, Weihua He, Fei Liang, Zhen Cheng, Wenhui Wang
Summary: CFIMC is a camera-free technique that allows real-time measurement of mechanical properties of single cells. It has high throughput and can reveal mechanical differences among different types of cells.
Article
Automation & Control Systems
Fei Liang, Peng Zhao, Yongxiang Feng, Wenhui Wang
Summary: This article presents a vision-based contact type liquid level detection method, which is capable of accurately detecting the liquid level of small volume samples. The method utilizes common hardware settings for micromanipulation and records ripple patterns to determine the contact moment. The experiments revealed a detection accuracy of 40 nm and robustness to various micromanipulation settings.
IEEE TRANSACTIONS ON INDUSTRIAL ELECTRONICS
(2022)
Article
Engineering, Biomedical
Peng Zhao, Simin Cheng, Yongxiang Feng, Fei Liang, Xinrong Zhang, Xiaoxiao Ma, Wenhui Wang
Summary: This study developed an automated and miniaturized picoliter extraction system for single-cell mass spectrometry analysis, which achieved better performance in consistency, sensitivity, and success rate compared to manual operation. The system lays a solid basis for applying picoliter electrospray ionization MS analysis in automated and high-throughput single cell metabolomics and lipidomics.
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2023)
Article
Chemistry, Analytical
Qiang Fang, Yongxiang Feng, Junwen Zhu, Liang Huang, Wenhui Wang
Summary: In this study, a novel microdevice was designed to accurately determine the spatial position of single cells by measuring the induced current generated by the combined action of the floating electrode and the differential electrodes. The device was experimentally validated to achieve precise localization of yeast cells and particles, and simultaneously characterize their velocity and size.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Peng Zhao, Yongxiang Feng, Junhan Wu, Junwen Zhu, Jinlei Yang, Xiaoxiao Ma, Zheng Ouyang, Xinrong Zhang, Wenpeng Zhang, Wenhui Wang
Summary: Mass spectrometry (MS) has become a powerful tool for analyzing metabolome, lipidome, and proteome. However, analyzing multi-omics in single cells is still challenging. In this study, a streamlined strategy for efficient and automatic single-cell multi-omics analysis by MS was developed. The strategy includes the use of a microwell chip for housing single cells, an automated system for extracting metabolites, phospholipids, and proteins, and obtaining MS2 spectra from a single cell sample. The strategy was successfully applied to analyze cancer tissue samples and improved cell classification accuracy compared to single-omics analysis.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Electrical & Electronic
Wenhui Wang, Xingke Zhao, Zenglei Zhao, Yuhan Rong
Summary: In this study, tungsten ball BGA packaging was proposed to reduce the CTE mismatch between packaging materials. Core-shell-structured W@Cu balls were fabricated using copper electroplating. Single-ball joint specimens were made and thermal aging treatments were conducted. The results show that the shear strength decreases after thermal aging and the formation and roughness of Cu6Sn5 IMC layer were observed.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Huichao Chai, Yongxiang Feng, Junwen Zhu, Xiaoran Meng, Fei Liang, Jingwei Bai, Wenhui Wang
Summary: The electrical properties of single cells are important biomarkers for disease and immunity. Impedance flow cytometry (IFC) provides a means to characterize the electrical properties of single cells on a high throughput scale. This study proposes a method to evaluate the accuracy of the single-cell electrical model using cell-sized unilamellar liposomes. Experimental results show that the relative error of the electrical parameters measured by IFC is less than 10% when the size of the sensing zone is close to the measured particles. This method can contribute to the improvement of IFC and the accurate characterization of single cells.
Article
Chemistry, Physical
Fei Liang, Junwen Zhu, Huichao Chai, Yongxiang Feng, Peng Zhao, Shaofeng Liu, Yuanmu Yang, Linhan Lin, Liangcai Cao, Wenhui Wang
Summary: Non-invasive and rapid imaging technique at subcellular resolution is significantly important for multiple biological applications. This study presents a long-term 3D refractive-index imaging system that integrates a cutting-edge white light diffraction phase microscopy module and an acoustofluidic device for single cell culture. The system allows efficient cell identification and enables long-term and frequency-on-demand 3D imaging of cancer cells, providing insights into cell growth, apoptosis, and necrosis. Overall, the proposed imaging technique opens up new avenues for visualizing intracellular structures and has potential applications in disease diagnosis and nanomedicine.
Article
Chemistry, Multidisciplinary
Yongxiang Feng, Junwen Zhu, Huichao Chai, Weihua He, Liang Huang, Wenhui Wang
Summary: This study proposes an impedance-based multimodal electrical-mechanical flow cytometry framework for high-dimensional intrinsic measurement of single cells. By leveraging the spatial-temporal characteristics of impedance sensing signal, this framework accurately characterizes the physiological states and phenotypes of single cells. Experimental results demonstrate high classification accuracy in distinguishing cell types and pharmacological perturbations. This multimodal approach provides a new perspective for accurate label-free single-cell intrinsic characterization.
Article
Biochemical Research Methods
Junwen Zhu, Yongxiang Feng, Huichao Chai, Fei Liang, Zhen Cheng, Wenhui Wang
Summary: As a label-free and high-throughput single cell analysis platform, impedance flow cytometry (IFC) can suffer from clogging issues. This study proposes the use of non-conductive viscous sheath flow in current sheath constriction (SC) solutions to improve sensitivity and throughput while maintaining accuracy. By placing mechanical constriction (MC) and SC in series in the same microfluidic chip, the hypothesis is proven through modeling analysis and experiments. The results show that viscous non-conductive PEG solution improves sensitivity and signal-to-noise ratio in impedance measurement, without clogging, while also demonstrating the ability to distinguish different types of cancer cells and subtypes of human breast cancer cells.
Article
Nanoscience & Nanotechnology
Shuai Zhang, Yue Wang, Chaoqiang Yang, Junwen Zhu, Xiongying Ye, Wenhui Wang
Summary: This paper presents an integrated microfluidic chip for isolating rare circulating tumor cells (CTCs) from whole blood. The chip consists of two stages of separation, with the first stage removing blood cells through filtration and the second stage capturing CTCs using dense immuno-magnetic-bead clumps. Both the filtration and capture methods are validated and optimized experimentally, achieving high recovery rate and purity for rare cancer cells in whole blood.
NANOTECHNOLOGY AND PRECISION ENGINEERING
(2022)