Non-templated addition and template switching by Moloney murine leukemia virus (MMLV)-based reverse transcriptases co-occur and compete with each other

Template-primer binding affinity and RNase H cleavage specificity contribute to the strand transfer efficiency of HIV-1 reverse transcriptase

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Unsupervised Trajectory Analysis of Single-Cell RNA-Seq and Imaging Data Reveals Alternative Tuft Cell Origins in the Gut

(2018) Charles A. Herring et al.   Cell Systems

SMRT-Cappable-seq reveals complex operon variants in bacteria

(2018) Bo Yan et al.   Nature Communications

Comparative Analysis of Single-Cell RNA Sequencing Methods

(2017) Christoph Ziegenhain et al.   MOLECULAR CELL

Single-cell transcriptomics to explore the immune system in health and disease

(2017) Michael J. T. Stubbington et al.   SCIENCE

Efficient N-tailing of blunt DNA ends by Moloney murine leukemia virus reverse transcriptase

(2017) Yoshiyuki Ohtsubo et al.   Scientific Reports

Compounds that enhance the tailing activity of Moloney murine leukemia virus reverse transcriptase

(2017) Yoshiyuki Ohtsubo et al.   Scientific Reports

A cost effective 5΄ selective single cell transcriptome profiling approach with improved UMI design

(2016) Marie-Jeanne Arguel et al.   NUCLEIC ACIDS RESEARCH

cDNA Library Enrichment of Full Length Transcripts for SMRT Long Read Sequencing

(2016) Maria Cartolano et al.   PLoS One

Benchmarking of the Oxford Nanopore MinION sequencing for quantitative and qualitative assessment of cDNA populations

(2016) Spyros Oikonomopoulos et al.   Scientific Reports

NanoCAGE-XL and CapFilter: an approach to genome wide identification of high confidence transcription start sites

(2015) Jason S. Cumbie et al.   BMC GENOMICS

Adapting capillary gel electrophoresis as a sensitive, high-throughput method to accelerate characterization of nucleic acid metabolic enzymes

(2015) Lucia Greenough et al.   NUCLEIC ACIDS RESEARCH

Bias in Ligation-Based Small RNA Sequencing Library Construction Is Determined by Adaptor and RNA Structure

(2015) Ryan T. Fuchs et al.   PLoS One

Efficient Solid-Phase Synthesis of pppRNA by Using Product-Specific Labeling

(2014) M. Goldeck et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Single-cell RNA-seq: advances and future challenges

(2014) Antoine-Emmanuel Saliba et al.   NUCLEIC ACIDS RESEARCH

Base Preferences in Non-Templated Nucleotide Incorporation by MMLV-Derived Reverse Transcriptases

(2014) Pawel Zajac et al.   PLoS One

Smart-seq2 for sensitive full-length transcriptome profiling in single cells

(2013) Simone Picelli et al.   NATURE METHODS

Biases in small RNA deep sequencing data

(2013) Carsten A. Raabe et al.   NUCLEIC ACIDS RESEARCH

Identification and remediation of biases in the activity of RNA ligases in small-RNA deep sequencing

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Incorporation of non-natural nucleotides into template-switching oligonucleotides reduces background and improves cDNA synthesis from very small RNA samples

(2010) Jeremy Kapteyn et al.   BMC GENOMICS

Reverse transcriptases can clamp together nucleic acids strands with two complementary bases at their 3′-termini for initiating DNA synthesis

(2010) Iris Oz-Gleenberg et al.   NUCLEIC ACIDS RESEARCH

A guide for in-house design of template-switch-based 5′ rapid amplification of cDNA ends systems

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RNA-Seq: a revolutionary tool for transcriptomics

(2008) Zhong Wang et al.   NATURE REVIEWS GENETICS