Article
Biochemistry & Molecular Biology
Hsiang-Pu Feng, Hsiao-Yun Cheng, Ting-Feng Hsiao, Tai-Wei Lin, Jia-Wei Hsu, Lien-Hung Huang, Chia-Jung Yu
Summary: ADP-ribosylation factors (Arfs) and Arf-like (Arl) GTPases are crucial regulators of intracellular vesicle trafficking and Golgi structure. In this study, it was found that ArfGAP1 can promote GTP hydrolysis of Arl1, affecting its function at the trans-Golgi network (TGN).
Article
Cell Biology
Kuan-Jung Chen, Jia-Wei Hsu, Fang-Jen S. Lee
Summary: AMP-activated protein kinase (AMPK) functions as a noncanonical guanine-nucleotide-exchange factor (GEF) for Arf6 in a kinase-independent manner, therefore playing a crucial role in regulating cancer cell migration and invasion. The activation of Arf6 by AMPK occurs during glucose starvation and 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR) treatment, involving the C-terminal regulatory domain of AMPK.
JOURNAL OF CELL SCIENCE
(2022)
Article
Cell Biology
Kana Fujibayashi, Joji Mima
Summary: Arf-family small GTPases play crucial roles in membrane trafficking and tethering processes. Arf6 can act as a membrane tether by physically linking distinct lipid bilayers, while Arf1 exhibits weaker membrane tethering capability under current experimental conditions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Huijie Zhao, Ziam Khan, Christopher J. Westlake
Summary: Ciliogenesis is a complex process involved in the assembly of cilia and flagella, which are essential for cell motility and signaling. Membrane trafficking regulators play a crucial role in ciliogenesis by organizing membranes around microtubule-based structures. The association of membranes with the distal end of the centriole is a critical step in initiating ciliogenesis. This review focuses on recent insights into cilia biogenesis and the roles of membrane trafficking regulators in ciliogenesis mechanisms relevant to human disease.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Manfei Li, Ran Zhao, Yanfang Du, Xiaomeng Shen, Qiang Ning, Yunfu Li, Dan Liu, Qing Xiong, Zuxin Zhang
Summary: This study characterized the AGAP-recruited complex and its roles in maize cellular physiology and agronomically important traits. The coordinated KNR6-AGAP-ARF1 complex modulates vegetative and reproductive traits by participating in vesicle trafficking in maize, providing insight into the regulation of inflorescence development, ear length, and kernel number in maize.
Review
Biochemistry & Molecular Biology
Qingchun Lu, Po-Shun Wang, Ling Yang
Summary: This review focuses on highlighting Golgi-associated Rab GTPases as master regulators of autophagic flux, discussing the future research prospects in elucidating their role and mechanism in autophagy and autophagy-related diseases.
CELL AND BIOSCIENCE
(2021)
Article
Cell Biology
Katharina Korbula, Iana Hammerschmid, Johannes Lesigang, Gang Dong
Summary: The exocyst is a protein complex that tethers secretory vesicles to the plasma membrane before their fusion, and it is involved in various cellular processes. One of its subunits, Sec8, interacts with synaptic scaffolding proteins SAP102 and PSD-95. In this study, the crystal structure of the C-terminal half of rat Sec8 was determined, revealing unique features of Sec8 and its binding preference for SAP102's PDZ2 domain. The findings provide insights into the interaction between Sec8 and SAP102.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Alison Boutet, Carlos Zeledon, Gregory Emery
Summary: Chemotaxis is important for diverse migrations in development and diseases like cancer progression. In this study, the researchers investigated the role of ArfGAP1 in regulating chemotaxis during collective cell migration. They found that ArfGAP1 is required to maintain receptor tyrosine kinases at the plasma membrane, and its absence leads to decreased active receptors and increased late endosomes. They also observed increased late endosomes and lysosomes in the absence of ArfGAP1, and identified genetic interactions with Lrrk in receptor sorting.
Review
Biochemistry & Molecular Biology
Akiko Ito, Masahiro Fukaya, Hirotsugu Okamoto, Hiroyuki Sakagami
Summary: The cytohesin-Arf pathway plays important physiological and pathological roles in neurons, involving various neuronal functions and related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Yongzhe Zhu, Xiaohang Wang, Zhiwei He, Ping Zhao, Hao Ren, Zhongtian Qi
Summary: Infection of the central nervous system caused by enterovirus 71 (EV71) in hand-foot-and-mouth disease is the main cause of death. This study identified a new pathway by which EV71 enters human brain microvascular endothelial cells (HBMECs) and showed its dependence on ADP-ribosylation factor 6 (ARF6). Inhibition of ARF6 reduced EV71 infectivity in HBMECs and a specific inhibitor of ARF6 alleviated EV71 infection mortality in mice.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Review
Biochemistry & Molecular Biology
Alazne Arrazola Sastre, Miriam Luque Montoro, Hadriano M. Lacerda, Francisco Llavero, Jose L. Zugaza
Summary: Rab and Arf GTPases play crucial roles in the pathogenesis and progression of neurodegenerative diseases, making them potential therapeutic targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Kunli Zhao, Kohichi Matsunaga, Kouichi Mizuno, Hao Wang, Katsuhide Okunishi, Tetsuro Izumi
Summary: The Rab27 effectors, including exophilin-8, granuphilin, and melanophilin, play different roles in regulated exocytosis in pancreatic beta cells. This study explores their functional relationships and the intracellular pathways involved in granule exocytosis. It is found that melanophilin functions downstream of exophilin-8 to mobilize granules for fusion with the plasma membrane. The exocyst complex physically links these effectors and is necessary for granule exocytosis when exophilin-8 is present.
Review
Cell Biology
I. -Ying Kuo, Chih-Hsiung Hsieh, Wan-Ting Kuo, Chih-Peng Chang, Yi-Ching Wang
Summary: Cells in the tumor microenvironment require a class of checkpoints to regulate various secretion and transport processes. Recent studies have shown that Rab proteins mediate communication between diverse cargoes and substrates through vesicular trafficking and secretion pathways in the tumor microenvironment. This review focuses on the role and regulation of vesicular secretion pathways in the tumor microenvironment, and describes the latest research advances and future directions.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Oncology
Jeong-In Park, Kyung-Hee Song, Seong-Mook Kang, Jeeyong Lee, Seong-Jun Cho, Hyun Kyung Choi, Jiyeon Ahn, Jong-Kuk Park, Jaesung Kim, Sang-Gu Hwang, Dae-Seog Lim, Joon Kim, Seung-Youn Jung, Jie-Young Song
Summary: This study demonstrates that BHMPS inhibits migration and invasion of breast cancer cells by blocking the interaction between Rab27a and Slp4, interfering with vesicle trafficking and secretion. BHMPS also decreases the expression of epithelial-mesenchymal transition transcription factors by inhibiting FAK and JNK activation, and reduces the secretion of epidermal growth factor receptor and fibronectin. In addition, BHMPS suppresses tumor growth in Rab27a-overexpressing MDA-MB-231 xenograft mice.
Article
Biochemistry & Molecular Biology
Xiaoyun Tan, Haoyue Xu, Jie Ye, Jin Wang, Wenlong Liu, Feng Liu, Qingchen Rui, Yiqun Bao
Summary: The exocyst subunit SEC6 plays an important role in cell plate formation during pollen mitosis I and might help to tether the vesicles before fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Mathematical & Computational Biology
Steven D. Lauzon, Viswanathan Ramakrishnan, Paul J. Nietert, Jody D. Ciolino, Michael D. Hill, Wenle Zhao
STATISTICS IN MEDICINE
(2020)
Article
Genetics & Heredity
Jordon B. Ritchie, Caitlin G. Allen, Heath Morrison, Michelle Nichols, Steven D. Lauzon, Joshua D. Schiffman, Chanita Hughes Halbert, Brandon M. Welch
MOLECULAR GENETICS & GENOMIC MEDICINE
(2020)
Article
Genetics & Heredity
Caitlin G. Allen, Jordon B. Ritchie, Heath Morrison, Steven D. Lauzon, Michelle Nichols, Joshua D. Schiffman, Chanita Hughes Halbert, Brandon M. Welch
Summary: The study reveals that cancer genetic counselors face both technology-specific and discipline-specific challenges when adopting health IT tools, including issues related to the relative advantage, complexity, compatibility, trialability, and observability of the tools, as well as discipline-specific challenges. Some counselors are considering adopting or switching health IT tools, but common barriers to adoption include a lack of perceived need for change, organizational infrastructure, cost, and decision-making power.
JOURNAL OF GENETIC COUNSELING
(2021)
Article
Psychology, Clinical
Andreana Benitez, Steven Lauzon, Paul J. Nietert, Aimee McRae-Clark, Brian J. Sherman
ADDICTIVE BEHAVIORS
(2020)
Article
Multidisciplinary Sciences
Tianfang Wang, Sang-Ho Kwon, Xiao Peng, Severine Urdy, Zefu Lu, Robert J. Schmitz, Stephen Dalton, Keith E. Mostov, Shaying Zhao
Article
Endocrinology & Metabolism
Mengru Zeng, Jin Wen, Zhengwei Ma, Li Xiao, Yutao Liu, Sangho Kwon, Yu Liu, Zheng Dong
Summary: In diabetic kidney disease, FOXO1 phosphorylation leads to downregulation of RAB27B, resulting in decreased exosome secretion.
Review
Biochemistry & Molecular Biology
Sekyung Oh, Sang-Ho Kwon
Summary: This article discusses the challenges of diagnosing acute kidney injury (AKI) and the discovery and application of EVs as biomarkers, as well as the importance of EVs for treating AKI. Future research will focus on addressing the shortcomings of current EV-based AKI diagnosis and therapeutic applications, with the aim of bringing more innovation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ashish K. Solanki, Ehtesham Arif, Pankaj Srivastava, Christopher M. Furchtl, Bushra Rahman, Pei Wen, Avinash Singh, Lawrence B. Holzman, Wayne R. Fitzgibbon, Milos N. Budisavljevic, Glenn P. Lobo, Sang-Ho Kwon, Zhe Han, Matthew J. Lazzara, Joshua H. Lipschutz, Deepak Nihalani
Summary: NEPHRIN and NEPH1 act as receptor proteins for hepatocyte growth factor (HGF), undergo phosphorylation independently of other receptors, and are dephosphorylated by SH2 domain-containing protein tyrosine phosphatase-2. High affinity interactions between NEPHRIN/NEPH1 and HGF were observed, with HGF-induced phosphorylation playing a central role in podocyte repair.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Health Care Sciences & Services
Steven D. Lauzon, Wenle Zhao, Paul J. Nietert, Jody D. Ciolino, Michael D. Hill, Viswanathan Ramakrishnan
Summary: Minimization is a common method for controlling baseline covariate imbalance at the randomization phase of clinical trials, but it may not preserve allocation randomness as well as other methods, making it vulnerable to predictability and bias. In comparison, minimal sufficient balance method has been found to have the least impact on power and bias in covariate-adjusted analyses, making it a recommended alternative to minimization in clinical trials.
STATISTICAL METHODS IN MEDICAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Hee Kyung Lee, Byung Rho Lee, Tae Jin Lee, Chang Min Lee, Chenglong Li, Paul M. O'Connor, Zheng Dong, Sang-Ho Kwon
Summary: By studying rat and mouse kidney injury models, we found that extracellular vesicle-loaded tRNA and their m(1)A modification can reflect oxidative stress, and the mechanism of tRNA packaging into extracellular vesicles is associated with oxidative stress.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Byung Rho Lee, Tae Jin Lee, Sekyung Oh, Chenglong Li, Jin-Hyuk A. Song, Brendan Marshall, Wenbo Zhi, Sang-Ho Kwon
Summary: In this study, the authors developed an in situ labelling approach to identify protein cargo in exosomes, which avoids the need for exosome isolation. They expressed an engineered peroxidase variant fused to an exosome cargo protein, and induced biotinylation of nearby proteins for a short period of time. Mass spectrometry analysis revealed that oxidative stress causes the accumulation of ribosomal proteins in a specific subpopulation of exosomes.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Physiology
Sekyung Oh, Chang M. Lee, Sang-H. Kwon
Summary: Most cells release nano-sized particles called extracellular vesicles (EVs) containing macromolecules such as microRNA (miRNA). These EVs can travel to target cells and alter gene expression profiles through interaction between miRNAs and messenger RNAs (mRNAs). Urinary EVs (uEVs) carry specific miRNAs that reflect kidney health, and research is being conducted to understand their biological roles and use them in treating kidney diseases. This review discusses the biology of EVs and miRNA, their applications in the kidney, and future directions for research. Overall, this topic is rated 8 out of 10 for its importance.
COMPREHENSIVE PHYSIOLOGY
(2023)
Article
Cell Biology
Man J. Livingston, Ming Zhang, Sang-Ho Kwon, Jian-Kang Chen, Honglin Li, Santhakumar Manicassamy, Zheng Dong
Summary: Autophagy induces EGR1 to activate the MAPK/ERK signaling pathway in renal tubular cells, promoting the transcription of FGF2 and stimulating fibrogenesis in fibroblasts. FGF2 then enhances the proliferation of fibroblasts, exacerbating renal fibrosis.
Article
Biochemistry & Molecular Biology
Goon-Tae Kim, Shivani Devi, Amitesh Sharma, Kyung-Hee Cho, Su-Jung Kim, Bo-Rahm Kim, Sang-Ho Kwon, Tae-Sik Park
Summary: The study found that ER stress can regulate the de novo biosynthesis of sphingolipids through the activation of serine palmitoyltransferase, leading to negative effects on hepatic insulin response. Understanding the mechanisms underlying insulin resistance is crucial for new treatments for metabolic diseases such as diabetes.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)
Article
Pediatrics
Abdul R. Shahein, J. Antonio Quiros, Ricardo A. Arbizu, Candi Jump, Steven D. Lauzon, Susan S. Baker
PEDIATRIC GASTROENTEROLOGY HEPATOLOGY & NUTRITION
(2020)