Article
Multidisciplinary Sciences
Semra Zuhal Birol, Rana Fucucuoglu, Sertac Cadirci, Ayca Sayi-Yazgan, Levent Trabzon
Summary: This study utilized microfluidic channels with cavitations to mimic the shape of atherosclerotic blood vessels and investigated the changes in inflammatory molecules ICAM-1 and IL-8 in THP-1 cells under different shear stress levels. The results showed that 15 Pa shear stress significantly increased the expression of ICAM-1 gene and IL-8 release in THP-1 cells, while decreasing the adhesion between THP-1 cells and endothelial cells.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Darian Williams, Marwa Mahmoud, Renfa Liu, Aitor Andueza, Sandeep Kumar, Dong-Won Kang, Jiahui Zhang, Ian Tamargo, Nicolas Villa-Roel, Kyung-In Baek, Hwakyoung Lee, Yongjin An, Leran Zhang, Edward W. Tate, Pritha Bagchi, Jan Pohl, Laurent O. Mosnier, Eleftherios P. Diamandis, Koichiro Mihara, Morley D. Hollenberg, Zhifei Dai, Hanjoong Jo
Summary: Atherosclerosis primarily occurs in arterial regions exposed to disturbed blood flow, while regions exposed to stable flow are protected. The study discovered that KLK10, a protein sensitive to flow, is upregulated under stable flow conditions and downregulated under disturbed flow conditions. It acts as an anti-inflammatory, barrier-protective, and anti-atherogenic factor.
Article
Biotechnology & Applied Microbiology
Nesrine Bouhrira, Brandon J. DeOre, Peter A. Galie
Summary: This study aims to mimic physiological flow using a low-cost method and investigate the impact of flow separation on velocity and shear stress profiles in a three-dimensional structure. Experimental and computational simulations reveal periodic changes in shear stress and flow separation zones in the flow dynamics.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biochemical Research Methods
Hao Yang, Tao Chen, Yichong Hu, Fuzhou Niu, Xinyu Zheng, Haizhen Sun, Liang Cheng, Lining Sun
Summary: The function of vascular endothelial cells (ECs) is regulated by biochemical cues, cell-cell interactions, and fluid shear stress. A multi-layer microfluidic chip was developed to study the effects of flow rate and TNF-alpha on the mechanical properties of ECs. The results showed the importance of hemodynamics and the adverse impact of inflammation on the vascular endothelium.
Article
Engineering, Biomedical
Naim Yagiz Demir, Ibrahim Bozyel, Baran Erman, Dincer Gokcen, Memed Duman
Summary: The aim of this study was to stimulate platelets without using chemical stimulants. Two mechanical platelet activation methods were proposed: shear-induced platelet activation using a microfluidic chip and ultrasound-assisted platelet activation using a piezo-based device. The results showed that the microfluidic chip with 8-nodes achieved the highest platelet activation rate (72.7%), while the ultrasound-assisted device achieved a 32.4% activation rate. These devices can be used to enhance the effect of PRP treatment and reduce adverse immune reactions.
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2023)
Article
Oncology
Thomas R. Coughlin, Ali Sana, Kevin Voss, Abhilash Gadi, Upal Basu-Roy, Caroline M. Curtin, Alka Mansukhani, Oran D. Kennedy
Summary: Primary bone cancers like osteosarcoma are driven by bone cells with mutations, and their behavior can be influenced by mechanical stimuli in the tumor microenvironment. Fluid flow over the cells' surface was found to change the behavior of osteosarcoma cells, potentially reducing their harmful effects. Sensitivity of bone cells to mechanical changes suggests that modulation of fluid movement or stiffness levels within tumors could be a novel consideration in developing new treatments to prevent cancer progression.
Article
Cardiac & Cardiovascular Systems
Yu-Tsung Shih, Shu-Yi Wei, Jin-Hua Chen, Wei-Li Wang, Hsin-Yi Wu, Mei-Cun Wang, Chia-Yu Lin, Pei-Lin Lee, Chih-Yuan Lin, Hung-Che Chiang, Yu-Ju Chen, Shu Chien, Jeng-Jiann Chiu
Summary: This study reveals the regulation of vascular endothelial phosphoproteins induced by disturbed flow and their contribution to atherogenesis. The phosphorylation of vinculin at serine 721 induced by disturbed flow promotes endothelial permeability and atherosclerosis. The level of endothelial VCLS721p is a clinicopathological biomarker for atherosclerosis progression.
EUROPEAN HEART JOURNAL
(2023)
Article
Cell Biology
Ruotian Du, Dongyan Li, Meng Zhu, Lisha Zheng, Keli Ren, Dong Han, Long Li, Jing Ji, Yubo Fan
Summary: The mechanical microenvironment and cellular senescence play a vital role in the development of primary open-angle glaucoma. This study found that shear stress has a more significant effect on normal cells compared to senescent cells. Additionally, senescent cells show reduced extracellular matrix turnover after shear stress stimulation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Pei-Yu Chu, Han-Yu Hsieh, Pei-Shan Chung, Pai-We Wang, Ming-Chung Wu, Yin-Quan Chen, Jean-Chen Kuo, Yu-Jui Fan
Summary: The objective of this study is to develop a device that mimics the microfluidic system of human arterial blood vessels. The device combines fluid shear stress (FSS) and cyclic stretch (CS) resulting from blood flow and pressure. It enables real-time observation of dynamic morphological changes in cells under different flow fields and stretch. By understanding the effects of FSS and CS on endothelial cells (ECs), including alignment of cytoskeleton proteins with fluid flow and redistribution of paxillin, this study can contribute to the prevention and improvement of cardiovascular diseases.
Review
Multidisciplinary Sciences
Sara Barrasa-Ramos, Claire A. A. Dessalles, Mathieu Hautefeuille, Abdul I. I. Barakat
Summary: Understanding and controlling angiogenesis is crucial in fields such as oncology, cardiovascular disease, and tissue engineering. Mechanical factors play a fundamental role in angiogenesis and can be used to optimize the development of vascular networks. This review focuses on the effects of fluid and solid mechanical stimuli on the early stages of angiogenesis, and discusses the challenges in the field and potential solutions using in vitro and physics-based modeling.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2022)
Article
Multidisciplinary Sciences
Chian-Shiu Chien, Julie Yi-Shuan Li, Yueh Chien, Mong-Lien Wang, Aliaksandr A. Yarmishyn, Ping-Hsing Tsai, Chi-Chang Juan, Phu Nguyen, Hao-min Cheng, Teh-Ia Huo, Shih-Hwa Chiou, Shu Chien
Summary: The study identified the important role of N-6-methyladenosine (m(6)A) RNA methylation in mechanotransduction in endothelial cells, with METTL3 as a central player in response to hemodynamic forces and atherogenic stimuli. The METTL3-mediated RNA hypermethylation regulated up-regulation of the NLRP1 transcript and downregulation of the KLF4 transcript through the YTHDF1 and YTHDF2 m(6)A reader proteins. Knockdown of METTL3 via repetitive shRNA administration prevented the atherogenic process, NLRP3 up-regulation, and KLF4 down-regulation in an in vivo atherosclerosis model.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemical Research Methods
Muaz S. Draz, Diego Dupouy, Martin A. M. Gijs
Summary: The usage of microfluidics in automated and fast immunoassays has gained significant interest. However, challenges arise in reconciling laminar flow patterns with diffusion-limited mass transport. This study investigates the beneficial effect of acoustic agitation on the uniformity of immunostaining and explores the impact of reducing incubation times and reagent concentrations on immunoassay signals. Acoustofluidic mixing successfully reduces incubation time and enhances signal-to-background ratio for spatial immunostaining.
Article
Engineering, Environmental
Cheol Hun Yoo, Yuseung Jo, Jeong Han Shin, Soomin Jung, Jeong-Geol Na, Taewook Kang, Jong Suk Lee
Summary: Here, we report a rational strategy for fabricating dual-functional microfiltration (MF) membranes integrated with micropatterns and Ag nanoparticles (NPs) to achieve ultrahigh and durable fouling resistance.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Neha S. Dole, Jihee Yoon, David A. Monteiro, Jason Yang, Courtney M. Mazur, Serra Kaya, Cassandra D. Belair, Tamara Alliston
Summary: This study investigated a microRNA-dependent mechanism mediating the crosstalk between TGF-β and Wnt signaling in osteocytes exposed to fluid shear stress, revealing that miR-100 integrates the functions of these two essential pathways by antagonizing Wnt signaling.
Article
Biochemistry & Molecular Biology
Ahmad Chehaitly, Anne-Laure Guihot, Coralyne Proux, Linda Grimaud, Jade Aurriere, Benoit Legouriellec, Jordan Rivron, Emilie Vessieres, Clement Tetaud, Antonio Zorzano, Vincent Procaccio, Francoise Joubaud, Pascal Reynier, Guy Lenaers, Laurent Loufrani, Daniel Henrion
Summary: The reduction in mitochondrial fusion in mouse endothelial cells was found to impair the dilator response to shear stress, leading to excessive superoxide production and promoting greater atherosclerosis development.
Article
Cell Biology
Anna Sawicka, Avin Babataheri, Stephanie Dogniaux, Abdul I. Barakat, David Gonzalez-Rodriguez, Claire Hivroz, Julien Husson
MOLECULAR BIOLOGY OF THE CELL
(2017)
Article
Multidisciplinary Sciences
Anne-Clemence Vion, Marouane Kheloufi, Adel Hammoutene, Johanne Poisson, Juliette Lasselin, Cecile Devue, Isabelle Pic, Nicolas Dupont, Johanna Busse, Konstantin Stark, Julie Lafaurie-Janvore, Abdul I. Barakat, Xavier Loyer, Michele Souyri, Benoit Viollet, Pierre Julia, Alain Tedgui, Patrice Codogno, Chantal M. Boulanger, Pierre-Emmanuel Rautou
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Cardiac & Cardiovascular Systems
Kazuyuki Yahagi, Yi Yang, Sho Torii, Johanne Mensah, Roseann M. White, Marion Mathieu, Erica Pacheco, Masataka Nakano, Abdul Barakat, Tahmer Sharkawi, Michel Vert, Michael Joner, Aloke V. Finn, Renu Virmani, Antoine Lafont
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2017)
Editorial Material
Cell Biology
Marouane Kheloufi, Anne-Clemence Vion, Adel Hammoutene, Johanne Poisson, Juliette Lasselin, Cecile Devue, Isabelle Pic, Nicolas Dupont, Johanna Busse, Konstantin Stark, Julie Lafaurie-Janvore, Abdul I. Barakat, Xavier Loyer, Michele Souyri, Benoit Viollet, Pierre Julia, Alain Tedgui, Patrice Codogno, Chantal M. Boulanger, Pierre-Emmanuel Rautou
Article
Mathematics, Applied
Ezio Di Costanzo, Abdul I. Barakat, Giuseppe Pontrelli
ZAMM-ZEITSCHRIFT FUR ANGEWANDTE MATHEMATIK UND MECHANIK
(2018)
Article
Multidisciplinary Sciences
V. Chandran Suja, A. I. Barakat
SCIENTIFIC REPORTS
(2018)
Article
Biophysics
Hengdi Zhang, Zaiyi Shen, Brenna Hogan, Abdul Barakat, Chaouqi Misbah
BIOPHYSICAL JOURNAL
(2018)
Article
Mathematics, Applied
Ezio Di Costanzo, Abdul I. Barakat, Giuseppe Pontrelli
ZAMM-ZEITSCHRIFT FUR ANGEWANDTE MATHEMATIK UND MECHANIK
(2018)
Article
Biophysics
Brenna Hogan, Zaiyi Shen, Hengdi Zhang, Chaouqi Misbah, Abdul I. Barakat
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2019)
Article
Multidisciplinary Sciences
C. F. Natale, J. Lafaurie-Janvore, M. Ventre, A. Babataheri, A. Barakat
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2019)
Review
Cell Biology
Claire A. Dessalles, Avin Babataheri, Abdul Barakat
Summary: This article focuses on the role of mechanics and mechanobiology in regulating pericyte function, including their interactions with the basement membrane and endothelial cells, as well as their responses to forces. To better understand pericyte function and dysfunction, more evolved models are needed.
JOURNAL OF CELL SCIENCE
(2021)
Article
Engineering, Biomedical
Claire A. Dessalles, Clara Ramon-Lozano, Avin Babataheri, Abdul Barakat
Summary: In this study, a novel microvessel-on-chip system based on the templating technique and using luminal flow actuation was developed to generate physiologically relevant levels of wall shear stress and circumferential stretch. The poroelastic behavior of the hydrogel was found to play a crucial role in determining the magnitudes of the wall shear stress and strain. This research adds a new dimension to the capabilities of microvessel-on-chip systems and provides a versatile user manual for parameter selection.
Article
Biology
Vassanti Audemar, Yannick Guerringue, Joni Frederick, Pauline Vinet, Isaty Melogno, Avin Babataheri, Valerie Legue, Sebastien Thomine, Jean-Marie Frachisse
Summary: A study has investigated how plant roots respond to mechanical compression using a microfluidic system. The researchers found that lateral pressure on the roots resulted in moderate elastic deformation of the root cells and an increase in calcium signaling. They also observed that repeated pressure stimuli led to a decrease in the calcium signal. These findings contribute to our understanding of how roots adapt to mechanical cues in the soil.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)