Article
Developmental Biology
Junnan Fang, Dorothy A. Lerit
Summary: This study investigates the post-transcriptional regulation of the Drosophila Pericentrin-like protein (PLP) mRNA. The research reveals that the CPEB ortholog Orb interacts with Plp mRNA to promote polyadenylation and enhance PLP protein expression. Loss of Orb leads to decreased PLP levels, compromising PCM scaffolding and centrosome function, which results in genomic instability and embryonic lethality. Moreover, overexpression of PLP restores centrosome scaffolding and rescues cell division defects caused by Orb depletion.
Article
Biochemistry & Molecular Biology
Jun-Chao Jiang, Hua Zhang, Lan-Rui Cao, Xing-Xing Dai, Long-Wen Zhao, Hong-Bin Liu, Heng-Yu Fan
Summary: The study identified PAP alpha as the enzyme responsible for cytoplasmic mRNA polyadenylation in mouse oocytes, showing its crucial role in meiotic cell cycle progression. The activation of PAP alpha through phosphorylation by CDK1 and ERK1/2 enhances mRNA translation and polyadenylation, leading to improved meiotic cell cycle progression.
NUCLEIC ACIDS RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Estanislao Navarro, Adrian Mallen, Miguel Hueso
Summary: This paper discusses the polymorphic nature of 3'UTR regions in mRNAs and the mechanisms that control their length and sequence, impacting the accessibility of functional effectors and ultimately gene expression regulation.
Article
Cell Biology
Eugene N. Kozlov, Roman V. Deev, Elena V. Tokmatcheva, Anna Tvorogova, Zaur M. Kachaev, Rudolf A. Gilmutdinov, Mariya Zhukova, Elena V. Savvateeva-Popova, Paul Schedl, Yulii V. Shidlovskii
Summary: Intracellular trafficking plays a critical role in the functioning of highly polarized cells, such as neurons. Transport of mRNAs, proteins, and other molecules to synaptic terminals maintains contact between neurons and ensures the transmission of nerve impulses. Cytoplasmic polyadenylation element binding (CPEB) proteins play an essential role in long-term memory (LTM) formation by regulating local translation in synapses.
Article
Genetics & Heredity
Bongmin Bae, Pedro Miura
Summary: Alternative cleavage and polyadenylation (APA) is prevalent in more than 70% of human and mouse genes, with distal poly(A) site selection influencing thousands of genes in the nervous system. Using mouse embryonic stem cell-derived neurons as a model system, researchers demonstrated widespread 3' UTR lengthening that closely resembles APA patterns in mouse cortex. CRISPR/Cas9 editing allows for efficient elimination of long 3' UTR isoform expression, with Oxford Nanopore Technologies platform providing a cost-effective strategy to confirm specific loss of targeted isoforms.
FRONTIERS IN GENETICS
(2021)
Review
Biochemistry & Molecular Biology
Qiang Zhang, Bin Tian
Summary: The 3' untranslated region (3'UTR) of mRNA is crucial for post-transcriptional regulation of gene expression. Eukaryotic protein-coding genes express multiple 3'UTR isoforms due to alternative cleavage and polyadenylation (APA). Cell proliferation, differentiation, and stress conditions lead to changes in the 3'UTR isoform expression profile. This review focuses on the regulation of 3'UTR isoforms in cell metabolic reprogramming, specifically exploring cell growth and autophagy responses mediated by the mTOR pathway.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2023)
Article
Genetics & Heredity
Justinn Barr, Sofia Charania, Rudolf Gilmutdinov, Konstantin Yakovlev, Yulii Shidlovskii, Paul Schedl
Review
Biochemistry & Molecular Biology
Zaur M. Kachaev, Lyubov A. Lebedeva, Eugene N. Kozlov, Yulii V. Shidlovskii
BIOSCIENCE REPORTS
(2020)
Review
Biochemistry & Molecular Biology
Anna E. Pravednikova, Sergey Y. Shevchenko, Victor V. Kerchev, Manana R. Skhirtladze, Svetlana N. Larina, Zaur M. Kachaev, Alexander D. Egorov, Yulii V. Shidlovskii
MOLECULAR MEDICINE
(2020)
Article
Biochemistry & Molecular Biology
Tom J. Zajdel, Gawoon Shim, Linus Wang, Alejandro Rossello-Martinez, Daniel J. Cohen
Review
Cell Biology
Oleg Bylino, Airat N. Ibragimov, Yulii Shidlovskii
Review
Cell Biology
Airat N. Ibragimov, Oleg Bylino, Yulii Shidlovskii
Article
Biochemistry & Molecular Biology
Yulii V. Shidlovskii, Oleg V. Bylino, Alexander V. Shaposhnikov, Zaur M. Kachaev, Lyubov A. Lebedeva, Valeria V. Kolesnik, Diego Amendola, Giovanna De Simone, Nadia Formicola, Paul Schedl, Filomena Anna Digilio, Ennio Giordano
Summary: The chromatin remodeler SWI/SNF plays a key role in gene activation by opening chromatin structure on promoters and enhancers. A study on Drosophila showed that SWI/SNF factors, specifically the BAP170 and SAYP subunits of the PBAP subfamily, mediate enhancer-dependent transcription of a reporter gene. This suggests that SWI/SNF and its subunits are critical for establishing enhancer-mediated transcription.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Eugene Kozlov, Yulii V. Shidlovskii, Rudolf Gilmutdinov, Paul Schedl, Mariya Zhukova
Summary: CPEB proteins are crucial for posttranscriptional gene regulation, involving in mRNA transport, localization, and stability. They are evolutionally conserved and play similar roles in vertebrates and invertebrates, contributing to neural development and function.
CELL AND BIOSCIENCE
(2021)
Article
Developmental Biology
Rudolf Gilmutdinov, Eugene N. Kozlov, Konstantin Yakovlev, Ludmila Olenina, Alexei A. Kotov, Justinn Barr, Mariya Zhukova, Paul Schedl, Yulii Shidlovskii
Summary: Research has shown that the Drosophila CPEB protein Orb2 plays a key role in spermatogenesis, with its deletion resulting in abnormalities in spermatid differentiation but no impact on meiosis.
Review
Cell Biology
Zaur M. Kachaev, Sergey D. Ivashchenko, Eugene N. Kozlov, Lyubov A. Lebedeva, Yulii V. Shidlovskii
Summary: Components of the translation apparatus, including ribosomal proteins, play crucial roles in cell nucleus, involving in nuclear processes and regulation of gene expression. These components control intranuclear trafficking, regulate protein activity, and participate in cellular response to stimulation and stress.
Article
Biochemistry & Molecular Biology
Carla Bertapelle, Maria Rosaria Carillo, Nunzio Antonio Cacciola, Yulii V. Shidlovskii, Gianfranco Peluso, Filomena Anna Digilio
Summary: Huntington's disease is a neurodegenerative disorder caused by a gene mutation, and recent studies have found a link between mitochondrial dysfunction and its pathogenesis. Research has shown that pharmacological modification of the carnitine cycle may help improve symptoms.
Article
Cell Biology
Eugene N. Kozlov, Roman V. Deev, Elena V. Tokmatcheva, Anna Tvorogova, Zaur M. Kachaev, Rudolf A. Gilmutdinov, Mariya Zhukova, Elena V. Savvateeva-Popova, Paul Schedl, Yulii V. Shidlovskii
Summary: Intracellular trafficking plays a critical role in the functioning of highly polarized cells, such as neurons. Transport of mRNAs, proteins, and other molecules to synaptic terminals maintains contact between neurons and ensures the transmission of nerve impulses. Cytoplasmic polyadenylation element binding (CPEB) proteins play an essential role in long-term memory (LTM) formation by regulating local translation in synapses.