Article
Cell Biology
Eugene N. Kozlov, Roman V. Deev, Elena V. Tokmatcheva, Anna Tvorogova, Zaur M. Kachaev, Rudolf A. Gilmutdinov, Mariya Zhukova, Elena V. Savvateeva-Popova, Paul Schedl, Yulii V. Shidlovskii
Summary: Intracellular trafficking plays a critical role in the functioning of highly polarized cells, such as neurons. Transport of mRNAs, proteins, and other molecules to synaptic terminals maintains contact between neurons and ensures the transmission of nerve impulses. Cytoplasmic polyadenylation element binding (CPEB) proteins play an essential role in long-term memory (LTM) formation by regulating local translation in synapses.
Article
Biochemistry & Molecular Biology
Rosa Pascual, Carolina Segura-Morales, Manja Omerzu, Nicolas Bellora, Eulalia Belloc, Chiara Lara Castellazzi, Oscar Reina, Eduardo Eyras, Madelon M. Maurice, Alba Millanes-Romero, Raul Mendez
Summary: Regulation of gene expression at different cell cycle phases is crucial for accurate chromosome duplication and segregation, involving dynamic reprogramming at various levels. The CPEB-family of RNA-binding proteins plays a key role in coordinating translation regulation for meiotic progression and mitotic transitions.
Article
Biochemistry & Molecular Biology
Daisuke Kaida, Kenta Shida
Summary: Pre-mRNA splicing is a critical mechanism in gene expression in eukaryotes, and splicing inhibition can impact various cellular functions. This study reveals that the potent splicing inhibitor spliceostatin A (SSA) causes stabilization of the p27 protein by increasing the stability of CDKN1B mRNA. The 3' untranslated region of CDKN1B mRNA is involved in this stabilization process.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Estanislao Navarro, Adrian Mallen, Miguel Hueso
Summary: This paper discusses the polymorphic nature of 3'UTR regions in mRNAs and the mechanisms that control their length and sequence, impacting the accessibility of functional effectors and ultimately gene expression regulation.
Article
Medicine, Research & Experimental
Siwei Pan, Jiaming Zhu, Pengfei Liu, Qiaochu Wei, Siyu Zhang, Wen An, Yuxin Tong, Zhenguo Cheng, Funan Liu
Summary: The study suggests that FN1 3'-UTR has stronger prognostic implications than FN1 protein in gastric cancer patients and can be a better therapeutic target for constructing targeted drugs.
Article
Reproductive Biology
Chun-Ru Song, Ru-Jing Zhang, Fang-Rui Xue, Xiao-Jie Zhang, Xing-Yue Wang, Dui Sun, Kang Ding, Qi Yang, Xin-Yu Wang, Cheng-Guang Liang
Summary: In this study, sheep were used as an animal model to investigate the regulatory mechanisms of cis-elements in the cytoplasmic polyadenylation and translation of maternal mRNAs in domestic animal oocytes. The positioning and number of polyadenylation signal (PAS) and cytoplasmic polyadenylation element (CPE) in the 3'-UTR were found to affect the efficiency of translational control.
Article
Genetics & Heredity
Bongmin Bae, Pedro Miura
Summary: Alternative cleavage and polyadenylation (APA) is prevalent in more than 70% of human and mouse genes, with distal poly(A) site selection influencing thousands of genes in the nervous system. Using mouse embryonic stem cell-derived neurons as a model system, researchers demonstrated widespread 3' UTR lengthening that closely resembles APA patterns in mouse cortex. CRISPR/Cas9 editing allows for efficient elimination of long 3' UTR isoform expression, with Oxford Nanopore Technologies platform providing a cost-effective strategy to confirm specific loss of targeted isoforms.
FRONTIERS IN GENETICS
(2021)
Article
Biology
Clara Suner, Annarita Sibilio, Judit Martin, Chiara Lara Castellazzi, Oscar Reina, Ivan Dotu, Adria Caballe, Elisa Rivas, Vittorio Calderone, Juana Diez, Angel R. Nebreda, Raul Mendez
Summary: Chronic inflammation is a major cause of disease. This study reveals the counterbalancing actions of RNA-binding proteins CPEB4 and TTP in inflammation resolution. CPEB4 stabilizes anti-inflammatory transcripts, while TTP directs mRNA deadenylation. The activities of CPEB4 and TTP are regulated through MAPK signaling. Maintaining the balance between these two opposing mechanisms is crucial for efficient inflammation resolution.
Article
Biochemistry & Molecular Biology
Zheng Xu, Jie Zhao, Minjie Hong, Chenming Zeng, Shouhong Guang, Yunyu Shi
Summary: PUF-8 regulates the lifespan of C. elegans not only through MFF but also by modulating pqm-1-related pathways.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Zheng Xu, Jie Zhao, Minjie Hong, Chenming Zeng, Shouhong Guang, Yunyu Shi
Summary: The protein PUF-8 regulates the lifespan of Caenorhabditis elegans by modulating mitochondrial dynamics, as well as pathways related to pqm-1 and aging.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Genetics & Heredity
Chidiebere U. Awah, Yana Glemaud, Fayola Levine, Kiseok Yang, Afrin Ansary, Fu Dong, Leonard Ash, Junfei Zhang, Olorunseun O. Ogunwobi
Summary: Resistance to targeted therapy is a challenge in breast, lung, and colorectal cancers, leading to a large number of deaths annually. We found that ERBB2+ cancer cells have mRNA-stabilizing sequences on their 3'UTR. By manipulating these sequences, we successfully degraded ERBB2 transcripts and reduced protein levels in various cancer cell types, providing a new approach to control oncogenic signals where current therapies fail.
FRONTIERS IN GENETICS
(2023)
Article
Biology
Masatoshi Miyakoshi, Teppei Morita, Asaki Kobayashi, Anna Berger, Hiroki Takahashi, Yasuhiro Gotoh, Tetsuya Hayashi, Kan Tanaka
Summary: Glutamine synthetase (GS) is a crucial enzyme for nitrogen assimilation under nitrogen limiting conditions. This study reveals that an sRNA GlnZ and a transcriptional regulator Nac both play roles in repressing the expression of the sucA component of 2-oxoglutarate dehydrogenase, thereby controlling the metabolic flow in response to nitrogen availability.
Article
Biochemistry & Molecular Biology
Wing-Keung Chu, Li-Man Hung, Chun-Wei Hou, Jan-Kan Chen
Summary: The Yes-associated protein (YAP) is a transcriptional co-activator crucial for organ development and tumorigenesis, inhibited by the Hippo signaling pathway. In human cancer cells, alternative splicing of YAP 3' UTR generates a shorter mRNA with potential impact on YAP expression. PKC inhibitor affects the ratio of different YAP mRNA forms, implicating PKC in the regulation of YAP expression through alternative splicing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Katie M. Yergert, Caleb A. Doll, Rebecca O'Rouke, Jacob H. Hines, Bruce Appel
Summary: The text discusses the formation and growth process of myelin sheaths, as well as the impact of local mechanisms and mRNA localization on the maturation of myelin sheaths. The study found that consensus sequences in the 3'UTR can determine the enrichment of mRNA in myelin sheaths.
Article
Multidisciplinary Sciences
Valter Bergant, Daniel Schnepf, Niklas de Andrade Kraetzig, Philipp Hubel, Christian Urban, Thomas Engleitner, Ronald Dijkman, Bernhard Ryffel, Katja Steiger, Percy A. A. Knolle, Georg Kochs, Roland Rad, Peter Staeheli, Andreas Pichlmair
Summary: Bergant et al. provide evidence that Influenza A viruses cause alternative polyadenylation of host mRNAs, leading to an attenuated phenotype in mice. This may constitute a common immune evasion mechanism employed by a variety of pathogenic viruses.
NATURE COMMUNICATIONS
(2023)
Review
Genetics & Heredity
Vladimir E. Adashev, Alexei A. Kotov, Sergei S. Bazylev, Aleksei S. Shatskikh, Alexei A. Aravin, Ludmila Olenina
Summary: The study focuses on the testis-specific genetic system of Drosophila melanogaster and its role in speciation and reproductive isolation. The findings highlight the importance of the Ste-Su(Ste) system and the piRNA pathway in contributing to the reproductive isolation of D. melanogaster lineage from other species.
FRONTIERS IN GENETICS
(2021)
Review
Biochemistry & Molecular Biology
Eugene Kozlov, Yulii V. Shidlovskii, Rudolf Gilmutdinov, Paul Schedl, Mariya Zhukova
Summary: CPEB proteins are crucial for posttranscriptional gene regulation, involving in mRNA transport, localization, and stability. They are evolutionally conserved and play similar roles in vertebrates and invertebrates, contributing to neural development and function.
CELL AND BIOSCIENCE
(2021)
Article
Evolutionary Biology
Julia K. Zograf, Yulia A. Trebukhova, Vladimir V. Yushin, Konstantin Yakovlev
Summary: The study revealed the presence of MSPs in the enoplean nematode Enoplus brevis, showing filamentous structures in sperm similar to chromadorean nematodes. E. brevis MSPs exhibit lower homology to known proteins in rhabditids, indicating more distant evolutionary relationships between Enoplea and Chromadorea. This data suggests a need to reevaluate current views of MSP evolution within Nematoda.
ORGANISMS DIVERSITY & EVOLUTION
(2022)
Review
Cell Biology
Zaur M. Kachaev, Sergey D. Ivashchenko, Eugene N. Kozlov, Lyubov A. Lebedeva, Yulii V. Shidlovskii
Summary: Components of the translation apparatus, including ribosomal proteins, play crucial roles in cell nucleus, involving in nuclear processes and regulation of gene expression. These components control intranuclear trafficking, regulate protein activity, and participate in cellular response to stimulation and stress.
Review
Biochemistry & Molecular Biology
Alexei A. Kotov, Sergei S. Bazylev, Vladimir E. Adashev, Aleksei S. Shatskikh, Ludmila V. Olenina
Summary: The Y chromosome is a sex chromosome found in male animals and undergoes dynamic evolutionary changes. It is characterized by unique chromatin landscapes and is enriched in tandem repeats and transposons. Despite structural divergence, Y-linked genes are mainly associated with spermatogenesis and male-specific selection shapes the evolution of Y chromosomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Mariya Zhukova, Panagiotis Sapountzis, Morten Schiott, Jacobus J. Boomsma
Summary: Rhizobiales associated with Acromyrmex leaf-cutting ants have lost their ability to fix nitrogen but have biosynthesis pathways that enhance the mutualistic relationship between the ants and the fungus. These symbionts are important enough for the ant hosts to have evolved functional defense mechanisms.
FEMS MICROBIOLOGY ECOLOGY
(2022)
Article
Multidisciplinary Sciences
Yulia O. Kipryushina, Mariia A. Maiorova, Konstantin Yakovlev
Summary: This study aimed to evaluate the reliability of RT-qPCR in analyzing the levels of maternal transcripts in the sea urchin egg cortex. By selecting potential reference genes and ranking them, the study found that the ranking of transcripts differed between total RNA and mRNA samples, but Ubb was the most suitable reference gene in both cases. By validating the gene of interest Daglb-2, the study observed an increased level of Daglb-2 in the egg cortex, suggesting that the proposed RNA isolation method with subsequent quantitative RT-qPCR analysis can be used to determine the cortical association of transcripts in sea urchin eggs.
Article
Chemistry, Physical
Alexander V. Romashchenko, Dmitrii V. Petrovskii, Sergey Yu. Trotsky, Ksenia N. Morozova, Nina B. Illarionova, Maria V. Zhukova, Elena V. Kiseleva, Marina B. Sharapova, Daniil S. Zuev, Konstantin E. Kuper, Sergey Yu. Taskaev, Anna I. Kasatova, Dmitrii A. Kasatov, Olga I. Solovieva, Ivan A. Razumov, Ludmila A. Geriinskaya, Mikhail P. Moshkin, Yuri M. Moshkin
Summary: Nanoparticles can be transported via the nose-to-brain (N2B) route, and this study focused on the dynamics and regulation of N2B transport using metal oxide/hydroxide NPs as MRI contrasts. The research revealed the movement and transport mechanism of Mn3O4-NPs within neurons and their trans-synaptic transmission, which are dependent on neuronal activity and synaptic stability. These findings have important implications for diagnostics, functional neuroimaging, and controlling N2B drug delivery.
Review
Cell Biology
Konstantin V. V. Yakovlev
Summary: Unfertilized eggs of animals contain maternal mRNAs and proteins, which are required for the initial stages of embryogenesis. Translation of maternal mRNAs is activated after fertilization, but a low rate of protein synthesis is detected in unfertilized sea urchin eggs.
CELL BIOLOGY INTERNATIONAL
(2023)
Article
Cell Biology
Eugene N. Kozlov, Roman V. Deev, Elena V. Tokmatcheva, Anna Tvorogova, Zaur M. Kachaev, Rudolf A. Gilmutdinov, Mariya Zhukova, Elena V. Savvateeva-Popova, Paul Schedl, Yulii V. Shidlovskii
Summary: Intracellular trafficking plays a critical role in the functioning of highly polarized cells, such as neurons. Transport of mRNAs, proteins, and other molecules to synaptic terminals maintains contact between neurons and ensures the transmission of nerve impulses. Cytoplasmic polyadenylation element binding (CPEB) proteins play an essential role in long-term memory (LTM) formation by regulating local translation in synapses.
Article
Chemistry, Physical
Alexander V. Romashchenko, Dmitrii V. Petrovskii, Sergey Yu. Trotsky, Ksenia N. Morozova, Nina B. Illarionova, Maria V. Zhukova, Elena V. Kiseleva, Marina B. Sharapova, Daniil S. Zuev, Konstantin E. Kuper, Sergey Yu. Taskaev, Anna I. Kasatova, Dmitrii A. Kasatov, Olga I. Solovieva, Ivan A. Razumov, Ludmila A. Gerlinskaya, Mikhail P. Moshkin, Yuri M. Moshkin
Summary: Nanoparticles can be transported from the nose to the brain and tracked using MRI. Mn3O4-NPs were found to be readily taken up by olfactory neurons and transported through axons, synapses, and across the olfactory tract. The transport of NPs was dependent on neuronal activity, microtubule integrity, calcium channels, and receptors. Olfactory stimuli could modulate and reroute the N2B transport of Mn3O4-NPs, and this transmission was impaired in aging and Parkinson's disease.
Meeting Abstract
Biochemistry & Molecular Biology
K. V. Yakovlev, M. V. Zhukova, R. A. Gilmutdinov, E. N. Kozlov, Y. V. Shidlovskii, P. Schedl
