One‐shot optimization of multiple enzyme parameters: Tailoring glucose oxidase for pH and electron mediators

The Crucial Role of Methodology Development in Directed Evolution of Selective Enzymes

(2019) Ge Qu et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Influence of methionine residue position on oxidative stability of glucose oxidase from Aspergillus niger

(2019) Gordana Kovačević et al.   BIOCHEMICAL ENGINEERING JOURNAL

HotSpot Wizard 3.0: web server for automated design of mutations and smart libraries based on sequence input information

(2018) Lenka Sumbalova et al.   NUCLEIC ACIDS RESEARCH

Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform

(2018) Fuqiang Ma et al.   Nature Communications

P450-Catalyzed Regio- and Diastereoselective Steroid Hydroxylation: Efficient Directed Evolution Enabled by Mutability Landscaping

(2018) Carlos G. Acevedo-Rocha et al.   ACS Catalysis

Application of fourier transform and proteochemometrics principles to protein engineering

(2018) Frédéric Cadet et al.   BMC BIOINFORMATICS

A machine learning approach for reliable prediction of amino acid interactions and its application in the directed evolution of enantioselective enzymes

(2018) Frédéric Cadet et al.   Scientific Reports

Engineering 7β-Hydroxysteroid Dehydrogenase for Enhanced Ursodeoxycholic Acid Production by Multiobjective Directed Evolution

(2017) Ming-Min Zheng et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Epistasis in protein evolution

(2016) Tyler N. Starr et al.   PROTEIN SCIENCE

How mutational epistasis impairs predictability in protein evolution and design

(2016) Charlotte M. Miton et al.   PROTEIN SCIENCE

Improving the Monooxygenase Activity and the Regio- and Stereoselectivity of Terpenoid Hydroxylation Using Ester Directing Groups

(2016) Emma A. Hall et al.   ACS Catalysis

Multiparameter Optimization in Directed Evolution: Engineering Thermostability, Enantioselectivity, and Activity of an Epoxide Hydrolase

(2016) Guangyue Li et al.   ACS Catalysis

Ultra-High-Throughput Screening Method for the Directed Evolution of Glucose Oxidase

(2014) Raluca Ostafe et al.   CHEMISTRY & BIOLOGY

Computational tools for designing and engineering enzymes

(2014) Jiri Damborsky et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

The Importance of Additive and Non-Additive Mutational Effects in Protein Engineering

(2013) Manfred T. Reetz   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Directed Evolution and Structural Analysis of NADPH-Dependent Acetoacetyl Coenzyme A (Acetoacetyl-CoA) Reductase from Ralstonia eutropha Reveals Two Mutations Responsible for Enhanced Kinetics

(2013) Ken'ichiro Matsumoto et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Reengineered glucose oxidase for amperometric glucose determination in diabetes analytics

(2013) Erik Arango Gutierrez et al.   BIOSENSORS & BIOELECTRONICS

Enhancing the Efficiency of Directed Evolution in Focused Enzyme Libraries by the Adaptive Substituent Reordering Algorithm

(2012) Xiaojiang Feng et al.   CHEMISTRY-A EUROPEAN JOURNAL

Technology behind commercial devices for blood glucose monitoring in diabetes management: A review

(2011) Sandeep Kumar Vashist et al.   ANALYTICA CHIMICA ACTA

Thermostabilization of an esterase by alignment-guided focussed directed evolution

(2010) Helge Jochens et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Glucose oxidase — An overview

(2009) Sandip B. Bankar et al.   BIOTECHNOLOGY ADVANCES

Computational tools for designing and engineering biocatalysts

(2009) Jiri Damborsky et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Exploring protein fitness landscapes by directed evolution

(2009) Philip A. Romero et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

In the light of directed evolution: Pathways of adaptive protein evolution

(2009) J. D. Bloom et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Technology Behind Glucose Meters: Test Strips

(2008) Joachim Hönes et al.   Diabetes Technology & Therapeutics

How Protein Stability and New Functions Trade Off

(2008) Nobuhiko Tokuriki et al.   PLoS Computational Biology