Article
Chemistry, Analytical
Hector Martinez-Perez-Cejuela, Maria Maddalena Calabretta, Valerio Bocci, Marcello D'Elia, Elisa Michelini
Summary: ATP determination is commonly used for microbial contamination assessment in different settings. However, commercially available kits require controlled temperature storage and transportation, limiting their applicability. To address this issue, a new bioluminescence paper biosensor was developed by combining a new luciferase mutant with a metal-organic framework (MOF). This sensor showed improved bioluminescence signal and stability, and successfully analyzed ATP content in complex biological matrices with low detection limits. The developed sensor has potential applications in various fields requiring ATP monitoring.
Article
Chemistry, Analytical
Elham Karimi, Maryam Nikkhah, Saman Hosseinkhani
Summary: Using gold nanoparticles as an enzyme immobilizer can increase the activity of luciferases and improve signal stability in the presence of nanoparticles. The application of ATP in the firefly luciferase reaction increases luciferase activity and enhances detection limits.
Article
Chemistry, Analytical
Yacob Melman, Evgeny Katz, Oleh Smutok
Summary: This research describes the construction of a new L-phenylalanine biosensor system based on smartphone-adapted analysis of the fluorescence output. The developed biosensor is sensitive, selective, cheap, and simple to use, making it promising for routine analysis of L-phenylalanine.
MICROCHEMICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
D. B. Kuznetsov, A. Yu. Mironov, V. A. Neschislyaev, I. L. Volkhin, E. V. Orlova, A. D. Shilina
Summary: This article introduces a method to restore the lost indicator properties of whole-cell biosensors using electromagnetic radiation. The study shows that radiation parameters have an impact on biosensor luminescence, and different types of radiation can partially restore the indicator properties of biosensors. The mechanism responsible for the sensitizing effect of radiation is explained, involving changes in solvent dipoles and mobility of acceptor molecules, triggering a cascade of biochemical reactions.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Yaohua Li, Xinyu He, Weinan Zhu, Haoran Li, Wei Wang
Summary: Bioluminescence in bacteria serves as a powerful tool for detecting environmental pollutants and biological toxins due to its high sensitivity, rapid detection, and ease of operation. Advances in gene engineering and optical technology have enabled the successful expression of bacterial luciferase in various cells and tissues, offering diverse applications in bioluminescence imaging related to infectious diseases, cancer therapy, and stem cell tracking.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Antonia Lopreside, Laura Montali, Baojun Wang, Annalisa Tassoni, Maura Ferri, Maria Maddalena Calabretta, Elisa Michelini
Summary: This low-cost biosensor integrates three different biorecognition principles for orthogonal detection of mercury(II) on a three-leaf paper, providing a stable disposable device that can easily be inserted into a smartphone for mercury detection in water samples, with a detection limit reaching ppb levels.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Daniela Visaggio, Mattia Pirolo, Emanuela Frangipani, Massimiliano Lucidi, Raffaella Sorrentino, Emma Mitidieri, Francesca Ungaro, Andrea Luraghi, Francesco Peri, Paolo Visca
Summary: A bioluminescent whole cell-based biosensor was constructed for rapid, sensitive, and single-step quantification of the pyochelin siderophore produced by Pseudomonas aeruginosa in biological samples.
Article
Chemistry, Analytical
Maria Maddalena Calabretta, Denise Gregucci, Hector Martinez-Perez-Cejuela, Elisa Michelini
Summary: The new mutant luciferase BgLuc has desirable properties for bioanalytical applications, improving the performance of methods such as ATP detection, whole-cell biosensors, and viability assays.
Review
Chemistry, Analytical
Elena Efremenko, Olga Senko, Nikolay Stepanov, Olga Maslova, Galina Yu. Lomakina, Natalia Ugarova
Summary: Bioluminescent ATP-assay has a wide range of applications in various fields, including sanitary control, water quality monitoring, food industry, drug maintenance, and monitoring of biocatalysts in biotechnological processes. New areas of ATP sensing, such as synthetic microbial consortia, pesticide degradation, and control of biocorrosive processes, have also been developed.
Article
Biotechnology & Applied Microbiology
Mark Wienhold, Andrew Kirkpatrick, Tingting Xu, Steven Ripp, Gary Sayler, Dan Close
Summary: Technologies enabling on-site environmental detection or medical diagnostics in resource-limited settings have a strong disruptive potential compared to current analytical approaches; experimental results show that while in vivo and in vitro systems are more sensitive, smartphone and night vision systems can also detect changes in cellular signal dynamics, indicating potential application value.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Yuan Zhang, Ying Yu, Xiaojun Yang, Xiaojun Yuan, Juan Zhang
Summary: A simple and low-cost paper-based microfluidic biosensor has been developed for on-site detection of Pb(II) using CRISPR-Cas12a assisted smartphone device. The biosensor exhibits high specificity, good anti-interference ability, stability, and applicability, making it a promising tool for on-site detection of Pb(II).
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Liping Huang, Wei Xiao, Tao Xu, Hui Chen, Zhiyuan Jin, Zhigang Zhang, Qifang Song, Yong Tang
Summary: A miniaturized paper-based smartphone biosensor detection system was developed for differential diagnosis of wild-type pseudorabies virus infection versus vaccination immunization. The method demonstrated excellent sensitivity and selectivity, with a high agreement rate (98%) with the most widely used commercial gE-ELISA kit in laboratory evaluations, showing great promise as an on-site differential diagnosis tool.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Mohammad Javad Afshari, Cang Li, Jianfeng Zeng, Jiabin Cui, Shuwang Wu, Mingyuan Gao
Summary: This study reports a biocompatible, self-illuminating probe with second near-infrared emission for improving the cytotoxicity concerns, penetration depth, and spatiotemporal resolution of bioluminescence imaging. It has been successfully applied for in vivo tumor imaging in mice.
Article
Chemistry, Analytical
Farzaneh Shalileh, Hossein Sabahi, Mohammad Golbashy, Mehdi Dadmehr, Morteza Hosseini
Summary: A novel colorimetric paper-based urea biosensor was developed for the rapid and accurate detection of urea adulteration in milk. The biosensing mechanism relies on the color change of anthocyanin due to the urease-mediated hydrolysis of urea and pH increment. The biosensor showed promising results for the detection of urea in milk samples, even in the presence of other adulterants and interferents.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biophysics
Lanlan Zhou, Linling Zhang, Liu Yang, Wei Ni, Yong Li, Yunhua Wu
Summary: A DNA-guided bioluminescence strategy was developed for the detection of dysregulated circulating miRNAs with high sensitivity and reliability. This method allows smartphone-based miRNA assays, making it suitable for resource-limited regions, and accurately identifies abnormal miRNAs in clinical samples from lung cancer patients.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)