Review
Chemistry, Analytical
Gengjun Liu, Jing Liu, Hong Zhou, Haiyan Wang
Summary: This paper reviews the research progress of AFP biosensors with enhanced sensitivity and selectivity using nanoparticles. Representative examples are presented to demonstrate the applications of nanotechnology in the early detection of AFP. The clinical application of AFP biosensors based on nanotechnology faces challenges, but also presents development opportunities in the future.
Article
Chemistry, Analytical
Huiyue Li, Xian Wang, Xue Zhang, Meng He, Jintao Zhang, Pan Liu, Xue Tang, Chunya Li, Yanying Wang
Summary: An ionic liquid with a sizeable heterocyclic structure was synthesized and used as a linker to coordinate with Eu3+ ions, forming metal-organic framework nanorods. By modifying with gold nanoparticles, the photoelectrochemical performance was enhanced, and a novel immunosensor for AFP detection was fabricated.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Boheng Dong, Tingting Sun, Xiang Jiang, Pengran Guo, Guofen Yang, Fuxian Wang
Summary: A novel CuV2O6 photoelectrode with self-passivation ability has been developed, showing superior stability under the testing environment. This material has great potential as a universal photoelectrode for reliable and accurate PEC detection of macromolecules, such as CEA and AFP.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Patrawadee Yaiwong, Siriporn Anuthum, Padchanee Sangthong, Jaroon Jakmunee, Suwussa Bamrungsap, Kontad Ounnunkad
Summary: This study presents the development of a simple, reliable, highly sensitive, and label-free aptasensor based on polyethyleneimine-coated gold nanoparticles (PEI-AuNPs) for the quantification of alpha-fetoprotein (AFP). The aptasensor is fabricated using a disposable ItalSens screen-printed electrode (SPE) and detects AFP concentration through the electrochemical response of toluidine blue (TB) intercalating into the aptamer-modified electrode after binding with the target. The assay exhibits a linear range of detection of 10 to 50000 pg mL(-1) with a limit of detection (LOD) of 9.5 pg mL(-1) in human serum.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Electrochemistry
Qingshan Yang, Ping Wang, Feng Tang, Shujun Wang, Zhe Zhao, Yao Wang, Yueyun Li, Qing Liu, Yunhui Dong
Summary: In this work, a label-free electrochemical immunosensor was developed for effective detection of alpha fetal protein (AFP). The sensor exhibited a satisfactory detection limit and a wide detection range for AFP detection. It demonstrated excellent performance in sensitivity, stability, selectivity, and reproducibility, making it suitable for practical application in clinical detection.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2021)
Review
Chemistry, Analytical
Dinh Tran Ngoc Huy, A. Heri Iswanto, Maria Jade Catalan Opulencia, Fahad Al-Saikhan, Anton Timoshin, Azher M. Abed, Irfan Ahmad, Sofiya A. Blinova, Ali Thaeer Hammid, Yasser Fakri Mustafa, Pham Van Tuan
Summary: Early diagnosis of hepatocellular carcinoma (HCC) is crucial for successful treatment and increased survival rate. Alpha-fetoprotein (AFP) has been used as a potential biomarker for HCC and other cancers. However, traditional detection methods have technical limitations. Recent advancements in aptasensors and nano-aptasensors, utilizing nanomaterials, have improved the sensitivity of AFP detection.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Xinxin Mo, Yu Wang, Qiyou Xiao, Xitong Zhou, He Li
Summary: A novel photoelectrochemical biosensor based on hyperbranched titanium dioxide was successfully developed for the detection of AFP in serum, showing good linear range, detection limit, selectivity, stability, and sensitivity, which could improve the accuracy and sensitivity of clinical detection of AFP.
SURFACES AND INTERFACES
(2021)
Article
Food Science & Technology
Monica Jimenez-Carretero, Javier Rodriguez-Lopez, Cristina Ropero-Moreno, Juan Granada, Josemaria Delgado-Martin, Manuel Martinez-Bueno, Antonia Fernandez-Vivas, Concepcion Jimenez-Lopez
Summary: The development of improved systems for rapid detection of trace infectious agents is essential. Magnetic nanoparticles (MNPs) are an interesting alternative due to their large surface area for maximizing interaction with microorganisms and their magnetic susceptibility for concentration using an external magnetic field. This study synthesized biomimetic magnetic nanoparticles (BMNPs) using MamC protein and demonstrated their efficacy in concentrating and detecting bacteria without the need for post-production coating. The system achieved specific detection of Staphylococcus aureus down to 10 CFU/mL using qPCR, making it a cost-effective alternative for bacteria detection in fluid samples.
Article
Chemistry, Analytical
Ji Yoon Lim, Soo Suk Lee
Summary: In this study, we developed quartz crystal microbalance immunosensors for the sensitive detection of alpha-fetoprotein in human serum. By using gold and titanium dioxide nanoparticles, we employed three signal amplification strategies to enhance the detection capability of the QCM immunosensor. Gold staining of gold nanoparticles and photocatalytic silver staining of titanium dioxide nanoparticles were effective methods for size expansion and mass enhancement, respectively. The detection limits achieved using these signal amplification techniques were significantly improved compared to traditional assays.
Article
Chemistry, Analytical
Xinming Qin, Yu Pan, Jiachang Zhang, Jingru Shen, Chunya Li
Summary: An imidazolium based ionic liquid was prepared and used as an organic ligand for trapezoidal metal-organic frameworks (Zn-MOF) nanosheets. Gold nanoparticles (AuNPs) were integrated onto the surface of Zn-MOF nanosheets to create AuNPs@Zn-MOF nanocomposites. The resulting nanocomposites showed enhanced photocurrent response and were used as a platform for fabricating an immunosensor for alpha-fetoprotein (AFP). The photocurrent response of the immunosensor exhibited a linear relationship with the logarithm of AFP concentration, with a detection limit of 1.88 pg/mL. The photoelectrochemical immunosensor demonstrated excellent selectivity, sensitivity, stability, and reproducibility for AFP determination.
Article
Biochemistry & Molecular Biology
Nikita Yabbarov, Elena Nikolskaya, Maria Sokol, Mariia Mollaeva, Margarita Chirkina, Irina Seregina, Mikhail Gulyaev, Yury Pirogov, Rem Petrov
Summary: This study demonstrates a dual-targeting drug delivery strategy that enhances the therapeutic efficacy by simultaneously targeting tumor endothelium and cell surface receptors. By functionalizing the nanodevice with selectin ligand and alpha-fetoprotein receptor-binding peptide, the strategy increases the accumulation and antitumor effect of the drug in tumor tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Jing Li, Dali Liu, Donglei Zhou, Long Shao, Xu Chen, Hongwei Song
Summary: A novel label-free photoelectrochemical biosensor based on Au/CsxWO3 heterogeneous films was proposed in this study, showing low background noise and excellent sensitivity in detecting AFP. The biosensor exhibited a linear response to AFP concentration in the range of 0.01 ng/mL to 500 ng/mL with a low detection limit of 7 pg/mL, and also demonstrated excellent specificity, repeatability, and long-term stability in analyzing human serum samples, indicating promising clinical and biological applications in the future.
Review
Chemistry, Multidisciplinary
Yuzhi Qiu, Guijian Tan, Yuqian Fang, Si Liu, Yubin Zhou, Abhinav Kumar, Manoj Trivedi, Dong Liu, Jianqiang Liu
Summary: Nano-enzymes are nano materials that mimic the catalytic function of enzymes and serve as substitutes for natural enzymes due to their excellent properties. Metal-organic frameworks (MOFs) and their derivatives represent a promising class of nano-enzymes with potential applications in biomedicine.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Biophysics
Shuju Zhao, Nianzu Liu, Wenqi Wang, Zhenying Xu, Yumin Wu, Xiliang Luo
Summary: The study designed a novel antifouling peptide and an electrochemical biosensor for accurate detection of alpha-fetoprotein in human serum. By coupling the antifouling peptide with polyaniline, the biosensor showed high antifouling performance and stability, indicating a potential for practical applications in biofluids.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biotechnology & Applied Microbiology
Mariya B. Sokol, Nikita G. Yabbarov, Mariia R. Mollaeva, Margarita Chirkina, Murad D. Mollaev, Artur Zabolotsky, Sergey L. Kuznetsov, Elena D. Nikolskaya
Summary: This study successfully increased the safety profile of paclitaxel by designing paclitaxel-loaded nanoparticles rAFP3d-NP. rAFP3d-NP showed high loading content and moderate size, with appropriate release rate and prolonged blood circulation. The study also found significant tumor growth inhibition by rAFP3d-NP.
Article
Biochemical Research Methods
Amal S. Basaleh, Sheta M. Sheta
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2020)
Article
Polymer Science
Amal S. Basaleh, Sheta M. Sheta
Summary: Innovative optical approach for troponin I (cTn) cardiac biomarker monitoring was proposed using manganese metal-organic framework Mn-MOF, showing high sensitivity. The study confirmed the potential use of Mn-MOF as an optical biosensor for cTn detection in biological samples, even with interfering analytes, based on its photoluminescence properties.
JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS AND MATERIALS
(2021)
Article
Chemistry, Physical
Said M. El-Sheikh, Diaa Osman, Omnia Ali, Wafaa Gh Shousha, Madiha A. Shoeib, Sherif M. Shawky, Sheta M. Sheta
Summary: The study developed a novel label-free electrochemical biosensor for direct detection of unamplified HCV nucleic acid based on bimetallic metal-organic framework (Ag/Zn-MOF). The biosensor showed high sensitivity, low detection limit, wide detection range, low cost, and ease of use. The bimetallic MOF exhibited excellent catalytic activity for electrochemical signal amplification.
APPLIED SURFACE SCIENCE
(2021)
Review
Environmental Sciences
Dina M. El-Sherif, Mohamed Abouzid, Mohamed S. Gaballah, Alhassan Ali Ahmed, Muhammad Adeel, Sheta M. Sheta
Summary: Biosensors are analytical tools that transform bio-signals into observable responses. They are effective in early detection of SARS-CoV-2 infection, targeting viral antigens to assess clinical development and severity of infection. Biosensors are capable of on-site, fast, and sensitive detection of the target viral antigen, providing opportunities for early detection.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Biochemical Research Methods
Sheta M. Sheta, Said M. El-Sheikh
Summary: This article provides a quick overview of COVID-19, including its definition, transmission, diagnosis, and global situation. It highlights the advanced technologies based on nanomaterials and MOFs for viral control and diagnosis of the SARS-CoV-2 pandemic.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Materials Science, Multidisciplinary
Sheta M. Sheta, Salem R. Salem, Said M. El-Sheikh
Summary: Recently, the increase in infectious diseases and microbial drug resistance has caused serious health problems. To address the knowledge gaps and guide strategies in the field of antimicrobials, our goal is to develop a new effective antimicrobial drug with extended action time, multi-antimicrobial agents, low toxicity, and safe strategies. Metal-organic frameworks have shown promise as antimicrobial agents. In this study, a cost-effective Fe(III)-MOF was prepared using a reflux method, and exhibited excellent antimicrobial efficiency against bacteria, fungus, and yeast.
JOURNAL OF MATERIALS RESEARCH
(2022)
Article
Chemistry, Analytical
Said M. El-Sheikh, Sheta M. Sheta, Salem R. Salem, Mohkles M. Abd-Elzaher, Amal S. Basaleh, Ammar A. Labib
Summary: A practical biosensor based on Zn(II) metal-organic framework nanoparticles (Zn-MOFs-NPs) was successfully developed for the monitoring and measurement of prostate-specific antigen (PSA). The biosensor exhibited high sensitivity, selectivity, and stability, and showed no interference from other common analytes. The detection limit for PSA was 0.145 fg/mL, and a wide linear concentration range of 0.1 fg/mL-20 pg/mL was achieved.
Review
Chemistry, Multidisciplinary
Sheta M. Sheta, Mohamed A. Hamouda, Omnia I. Ali, A. T. Kandil, Reda R. Sheha, Said M. El-Sheikh
Summary: The nuclear industry is rapidly developing and effective management of nuclear waste and monitoring the nuclear fuel cycle are crucial. The presence of various radionuclides in the environment is a major concern, and the development of materials with high adsorption capacity and selectivity is essential. Metal-organic frameworks (MOFs) have emerged as promising materials for removing radioactive elements from water resources. This review provides an analysis of the potential of MOFs as adsorbents for purifying radionuclides, discussing fabrication methods, adsorption mechanisms, effectiveness in wastewater treatment, behavior under high radiation, and renewability.
Article
Chemistry, Multidisciplinary
Reda M. Mohamed, Said M. El-Sheikh, Mohammad W. Kadi, Ammar A. Labib, Sheta M. Sheta
Summary: In this study, a novel gold-zinc-salen metal-organic framework composite, Au-Zn-Sln-MOF, was synthesized using an organic nano-linker with Zn(NO3)(2)center dot 6H(2)O and HAuCl4 center dot 3H(2)O. Various analytical instruments and spectroscopic techniques were used to characterize the synthesized composite. A novel swab test instrument and a simple colorimetric approach based on the Au-Zn-Sln-MOF composite were developed for detecting hCG hormone, which are both easy to use.
Article
Environmental Sciences
Reda R. Sheha, Sheta M. Sheta, Mohamed A. Hamouda, Said M. El-Sheikh, A. T. Kandil, Omnia I. Ali
Summary: A facile modification method using oxalic acid was employed to prepare MTSr-OX MOF, which exhibited excellent capability for eliminating 152+154Eu radioisotopes. The prepared MOF had a rod-like structure with a BET surface area of 101.55 m2 g+1. Batch sorption experiments demonstrated that MTSr-OX-MOF showed superior effectiveness in the elimination of 152+154Eu, with a maximum sorption capacity of 234.72 mg g+1 at pH 3.5. Kinetics fitted with the pseudo-second-order model and the Langmuir model correctly described the sorption mechanism. The MTSr-OX-MOF demonstrated excellent removal performance for a wide range of radionuclides in real active waste.
JOURNAL OF ENVIRONMENTAL RADIOACTIVITY
(2023)
Article
Chemistry, Multidisciplinary
Mohamed A. Hamouda, Sheta M. Sheta, Reda R. Sheha, A. T. Kandil, Omnia I. Ali, Said M. El-Sheikh
Summary: In this study, a novel MTSr-MOF was prepared and its sorption efficiency towards Eu152+154 radionuclides was improved through modification, showing promising potential for the removal of radioactive waste.
Correction
Chemistry, Multidisciplinary
Ahmed B. Azzam, Ridha Djellabi, Sheta M. Sheta, S. M. El-Sheikh
Article
Chemistry, Multidisciplinary
Ahmed B. Azzam, Ridha Djellabi, Sheta M. Sheta, S. M. El-Sheikh
Summary: In this study, BiPO4 nanorod bundles supported on g-C3N4 nanosheets as heterojunction composites were successfully synthesized through a facile chemical deposition method, aiming to convert 4-NP into 4-AP. The results showed that the reduction ability of BiPO4/g-C3N4 composites was superior to bare BiPO4 and g-C3N4, achieving complete conversion of 4-NP within one minute.
Article
Chemistry, Multidisciplinary
Sheta M. Sheta, Mohkles M. Abd-Elzaher, Said M. El-Sheikh
Summary: A chemosensor based on a nano-lanthanum complex derived from phenylenediamine was developed for the determination of macrofuran antibiotic (MFA), showing high sensitivity and selectivity.
