Journal
ANALYTICAL CHEMISTRY
Volume 91, Issue 23, Pages 14873-14878Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b02117
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [81673393]
- Shandong Natural Science Foundation [ZR2018ZC0233]
- Taishan Scholar Program at Shandong Province
- Qilu/Tang Scholar Program at Shandong University
- Key Research and Development Project of Shandong Province [2017CXGC1401]
Ask authors/readers for more resources
Fibroblast activation protein-alpha (FAP), as a crucial member of cell surface glycoprotein, highly expresses in reactive fibroblasts of tumors and several fibrosis diseases. It is a potential target for drug design and also reported as a prodrug strategy to increase the therapeutic window of some anticancer agents. In this work, we developed the first bioluminogenic probe for FAP with a limit-of-detection of 0.254 ng/mL, which could be applied to evaluate the FAP inhibitors in vitro. The experiments of transgenic mice and tumor-bearing nude mice validated our probe 1 could reflect the endogenous FAP level in vivo. Furthermore, this probe was successfully used to reflect FAP up-regulation in the lung homogenates of the bleomycin-induced idiopathic pulmonary fibrosis mice.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available