Article
Multidisciplinary Sciences
Xiqi Peng, Songsong Tang, Daitian Tang, Dewang Zhou, Yangyang Li, Qiwei Chen, Fangchen Wan, Heather Lukas, Hong Han, Xueji Zhang, Wei Gao, Song Wu
Summary: Researchers have developed an autonomous nanorobot capable of delivering drugs to mitochondria, which leads to improved anticancer effects and suppression of cancer cell metastasis. The nanorobot utilizes catalytic activity to decompose hydrogen peroxide in tumor cells, allowing effective delivery of drugs to mitochondria. In vivo experiments demonstrate the effectiveness of the nanorobot in subcutaneous and orthotopic breast tumor models. This breakthrough introduces a new era of precision therapy with organelle-level resolution.
Article
Pharmacology & Pharmacy
Cheng-Che Lee, Wei-Ting Chen, Syue-Yi Chen, Tsung-Ming Lee
Summary: This study demonstrated that dapagliflozin attenuates post-myocardial infarction arrhythmias by modulating AMP-activated protein kinase and SGLT1, reducing reactive oxygen species production, increasing connexin43 levels, and significantly improving arrhythmic severity.
BIOCHEMICAL PHARMACOLOGY
(2021)
Review
Instruments & Instrumentation
Tonggong Liu, Cheng Gao, Dayong Gu, Huanwen Tang
Summary: Cell-based drug delivery systems have advantages in improving drug transport efficiency and therapeutic efficacy. The construction methodology of cell-based carriers influences their physiological functions and clinical applications. Different types of cell-based carriers have been reported in recent years and their applications in disease therapy and challenges in clinical translation are discussed.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Tania Martins-Marques, Katja Witschas, Ilda Ribeiro, Monica Zuzarte, Steve Catarino, Teresa Ribeiro-Rodrigues, Francisco Caramelo, Trond Aasen, Isabel Marques Carreira, Lino Goncalves, Luc Leybaert, Henrique Girao
Summary: This study provides new evidence for the localization and functional role of connexin43 (Cx43) in the nucleus, particularly in transcription modulation. The findings show that Cx43 localizes to the nuclear envelope and impacts the cellular transcriptome by interacting with transcriptional regulators. The study also demonstrates that Cx43 forms active channels at the nuclear envelope, suggesting its involvement in nucleocytoplasmic shuttling. These findings have important implications for understanding the role of Cx43 in cardiac pathophysiology.
Article
Multidisciplinary Sciences
Shiyan Dong, Xuan Liu, Ye Bi, Yifan Wang, Abin Antony, Daeyong Lee, Kristin Huntoon, Seongdong Jeong, Yifan Ma, Xuefeng Li, Weiye Deng, Benjamin R. Schrank, Adam J. Grippin, Jonghoon Ha, Minjeong Kang, Mengyu Chang, Yarong Zhao, Rongze Sun, Xiangshi Sun, Jie Yang, Jiayi Chen, Sarah K. Tang, L. James Lee, Andrew S. Lee, Lirong Teng, Shengnian Wang, Lesheng Teng, Betty Y. S. Kim, Zhaogang Yang, Wen Jiang
Summary: This study demonstrates an adaptive strategy to dock different targeting ligands onto the surface of mRNA-loaded small extracellular vesicles (sEVs). These immunogenic sEVs (imsEV) can target glioblastoma cells and generate potent antitumour activities, including against tumours resistant to immunotherapy.
NATURE COMMUNICATIONS
(2023)
Review
Physiology
Daisuke Shimura, Robin M. Shaw
Summary: GJA1-20k is the primary gap junction protein in the mammalian heart ventricles, and is associated with cell-to-cell communication. Recent studies have shown that GJA1-20k plays a protective role in the mitochondria of myocardium, promoting ATP production, and interacts with the actin cytoskeleton.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Physiology
Haruka Sato, Masami Nishiyama, Natsuki Morita, Wakako Satoh, Taiki Hasegawa, Yuka Someya, Tsuyoshi Okumura, Sana Koyama, Chiyohiko Shindoh, Masahito Miura
Summary: Research shows that the lack of Cx43 can lead to the occurrence of triggered arrhythmias, mainly due to depolarization of mitochondrial membrane potential, increased mitochondrial Ca2+ and ROS, causing Ca2+ leak from the SR. Activation of mitochondrial K-ATP channels can partially counteract these effects.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Pei Huang, Hongzhang Deng, Changrong Wang, Yongfeng Zhou, Xiaoyuan Chen
Summary: Messenger RNA (mRNA)-based therapy is a powerful, safe, and rapidly scalable therapeutic approach. In this review, therapeutic applications of mRNA are introduced, common types of mRNA cargos and delivery systems are summarized, and strategies to enhance nanotechnology-mediated mRNA delivery efficiency during the cellular trafficking process are highlighted.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Wenxing Gu, Fenghua Meng, Rainer Haag, Zhiyuan Zhong
Summary: The article reviews the current status and prospects of targeted nanomedicines for cancer therapy, emphasizing their huge potential in treating various types of tumors and highlighting the importance of interdisciplinary collaborative research and development in becoming a key platform for precision cancer therapy.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Chemistry, Multidisciplinary
Jia-dan Wang, Ying Shao, Dan Liu, Nuo-ya Liu, Dan-yan Zhu
Summary: In this study, we elucidated the mechanisms of mitochondrial damage in ESC-CMs after knockdown of Rictor using a model of cardiomyocyte differentiation from mouse ES cells. Knockdown of Rictor led to swollen and ruptured mitochondria, decreased ATP production and mitochondrial transmembrane potential, inhibited mitochondrial respiratory chain complex activities, and hindered Cx43 translocation into mitochondria, ultimately resulted in mitochondrial dysfunction in ESC-CMs.
ACTA PHARMACOLOGICA SINICA
(2021)
Review
Biochemistry & Molecular Biology
Eline Simons, Bart Loeys, Maaike Alaerts
Summary: The discovery of induced pluripotent stem cells has allowed the generation of various cell types, including iPSC-derived cardiomyocytes, for different purposes such as disease modelling and drug discovery. While 2D iPSC-CM models have shown promise, they are less mature compared to adult cardiomyocytes. Novel approaches are being developed to create 3D models with added cell types, improving maturity and physiological relevance. This review focuses on the progress in modelling inherited cardiac arrhythmias using both 2D and 3D models, and their application in therapy development and drug testing.
Review
Biotechnology & Applied Microbiology
Olga Khorkova, Jack Stahl, Aswathy Joji, Claude-Henry Volmar, Claes Wahlestedt
Summary: Many diseases can be improved by therapies that increase gene expression, and RNA-targeted therapies are being developed to achieve this goal. The review discusses the use of nucleic acid-based therapeutics to target non-coding RNAs and regulate gene expression. Protein replacement and gene therapy have limitations, but the discovery of non-coding RNA networks provides alternative targets and strategies for increasing protein production.
NATURE REVIEWS DRUG DISCOVERY
(2023)
Article
Biochemistry & Molecular Biology
Yanbo Yang, Xiaohong Wu, Wenqian Yang, Weiwei Jin, Dongyang Wang, Jianye Yang, Guanghui Jiang, Wen Zhang, Xiaohui Niu, Jing Gong
Summary: This study systematically characterized the alternative polyadenylation (APA) profiles during the differentiation of induced pluripotent stem cells (iPSCs) to cardiomyocytes. Five dynamic APA patterns were identified, with progressive lengthening of the 30UTR being the main pattern over time. The genes associated with this pattern were enriched in cell cycle and mRNA metabolic process pathways. TIA1 was indicated to play an important role in regulating APA events with this pattern. Additionally, APA and polyA machinery activity at the early differentiation stage could respond to developmental signal-mediated stimuli and result in a sharp shortening of the 30UTR. Furthermore, a miRNA-APA network was constructed, and several hub miRNAs potentially regulating cardiomyocyte differentiation were identified.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Yuval Shemer, Lucy N. Mekies, Ronen Ben Jehuda, Polina Baskin, Rita Shulman, Binyamin Eisen, Danielle Regev, Eloisa Arbustini, Brenda Gerull, Mihaela Gherghiceanu, Eyal Gottlieb, Michael Arad, Ofer Binah
Summary: LMNA-related dilated cardiomyopathy is an inherited heart disease characterized by left ventricular enlargement and impaired systolic function caused by mutations in the LMNA gene, leading to abnormalities in cardiac physiology and gene expression. Studying induced pluripotent stem cell-derived cardiomyocytes from patients can provide insights into potential therapeutic strategies for the disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Arina Ranjit, Sana Khajeh Pour, Ali Aghazadeh-Habashi
Summary: This study investigates the anti-inflammatory effects of a synthetic peptide, novokinin, in rheumatoid arthritis (RA) and develops a bone-targeting conjugate to improve its stability and efficacy. It demonstrates that novokinin or the novel conjugate restores immune balance by modulating components of the Renin-Angiotensin System and arachidonic acid pathway, offering a potential alternative therapy for managing RA.
Review
Physiology
TingTing Hong, Robin M. Shaw
PHYSIOLOGICAL REVIEWS
(2017)
Article
Biochemistry & Molecular Biology
Bing Xu, Ying Fu, Yan Liu, Sosse Agvanian, Robert C. Wirka, Rachel Baum, Kang Zhou, Robin M. Shaw, TingTing Hong
Article
Physiology
Ying Fu, Shan-Shan Zhang, Shaohua Xiao, Wassim A. Basheer, Rachel Baum, Irina Epifantseva, TingTing Hong, Robin M. Shaw
FRONTIERS IN PHYSIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Irina Epifantseva, Robin M. Shaw
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2018)
Editorial Material
Cardiac & Cardiovascular Systems
Robin M. Shaw, Andriana P. Nikolova
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
(2018)
Editorial Material
Cardiac & Cardiovascular Systems
Andriana R. Nikolova, Robin M. Shaw
TRENDS IN CARDIOVASCULAR MEDICINE
(2018)
Editorial Material
Cardiac & Cardiovascular Systems
TingTing Hong, Robin M. Shaw
TRENDS IN CARDIOVASCULAR MEDICINE
(2019)
Editorial Material
Medicine, Research & Experimental
Robin M. Shaw, Jeffrey E. Saffitz
JOURNAL OF CLINICAL INVESTIGATION
(2020)
Article
Biochemistry & Molecular Biology
Irina Epifantseva, Shaohua Xiao, Rachel E. Baum, Andre G. Kleber, TingTing Hong, Robin M. Shaw
Editorial Material
Physiology
Muhammad S. Khan, Robin M. Shaw
JOURNAL OF GENERAL PHYSIOLOGY
(2021)
Article
Biology
Daisuke Shimura, Esther Nuebel, Rachel Baum, Steven E. Valdez, Shaohua Xiao, Junco S. Warren, Joseph A. Palatinus, TingTing Hong, Jared Rutter, Robin M. Shaw
Summary: In this study, it was found that GJA1-20k induces actin polymerization around mitochondria, leading to focal constriction sites and mitochondrial fission independent of canonical DRP1. The smaller mitochondria generated by GJA1-20k have reduced ROS generation and provide potent protection against ischemia-reperfusion injury in the heart. These results suggest that stress-responsive GJA1-20k stabilizes polymerized actin filaments to stimulate non-canonical mitochondrial fission which limits ischemia-reperfusion induced myocardial infarction.
Review
Cardiac & Cardiovascular Systems
Kevin S. Shah, Mary Elizabeth Hale Hammond, Stavros G. Drakos, Jeffrey L. Anderson, James C. Fang, Kirk U. Knowlton, Robin M. Shaw
Summary: SARS-CoV-2 has caused the global COVID-19 pandemic, leading to various cardiovascular complications in infected individuals. Understanding the pathophysiology, treatment options, and long-term risks for patients recovering from COVID-19 is crucial for future preparedness against similar health crises.
Letter
Cardiac & Cardiovascular Systems
Joseph A. Palatinus, Steven Valdez, Lindsey Taylor, Claire Whisenant, Craig H. Selzman, Stavros G. Drakos, Ravi Ranjan, TingTing Hong, Jeffrey E. Saffitz, Robin M. Shaw
CIRCULATION RESEARCH
(2023)
Article
Biochemical Research Methods
Daisuke Shimura, Robin M. Shaw
Summary: In this study, a protocol is presented for capturing actin-mediated mitochondrial fission using high-resolution time-lapse imaging, and the experimental steps are described in detail. This approach is also applicable for analyzing other cytoskeleton-mediated organelle events.
Article
Cardiac & Cardiovascular Systems
Wassim A. Basheer, Shaohua Xiao, Irina Epifantseva, Ying Fu, Andre G. Kleber, TingTing Hong, Robin M. Shaw
CIRCULATION RESEARCH
(2017)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)