Article
Immunology
Yingze Li, Guojian Fang, Wei Cao, Jiali Yuan, Shuai Song, Hong Peng, Yuepeng Wang, Qunshan Wang
Summary: In the aged and fibrotic left atrium, replicative senescence of atrial fibroblasts (AFs) plays a significant role in the pathogenesis of atrial fibrillation. The downregulation of Ezh2, a histone methyltransferase, is identified as a key regulator of AFs' replicative senescence. This finding may have implications for future treatments of atrial fibrosis in the elderly.
JOURNAL OF INFLAMMATION RESEARCH
(2022)
Article
Cardiac & Cardiovascular Systems
Diem Duong Ngoc Nguyen, Shamsul Mohd Zain, Mohd Hamzah Kamarulzaman, Teck Yew Low, William M. Chilian, Yan Pan, Kang Nee Ting, Aini Hamid, Arifah Abdul Kadir, Yuh-Fen Pung
Summary: The study focused on the intracellular and exosomal miRNA profiling in human vascular smooth muscle cells during replicative senescence. Specific dysregulated sets of miRNAs were identified from human vascular smooth muscle cells. Hsa-miR-155-5p was significantly downregulated in both intracellular and exosomal hVSMCs, suggesting its crucial role in cellular senescence. Hsa-miR-155-5p might be the mediator in linking cellular senescence to vascular aging and atherosclerosis.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Cell Biology
Fanyuan Yu, Lin Yao, Feifei Li, Chenglin Wang, Ling Ye
Summary: It has been found that YAP dysfunction, independent of Hippo signaling, can cause chronological aging of stromal cells by affecting the integrity of the nuclear envelope. Additionally, YAP activity also controls another type of cellular senescence, replicative senescence, in the in vitro expansion of mesenchymal stromal cells (MSCs), but this is dependent on Hippo phosphorylation and involves NE integrity-independent downstream mechanisms. The findings suggest that YAP plays a role in regulating replicative toxicity, genome stability, and DNA damage response/repair to maintain the regenerative capabilities of MSCs.
Article
Oncology
Liselot M. Mus, Stephane Van Haver, Mina Popovic, Wim Trypsteen, Steve Lefever, Nadja Zeltner, Yudelca Ogando, Eva Z. Jacobs, Geertrui Denecker, Ellen Sanders, Christophe Van Neste, Suzanne Vanhauwaert, Bieke Decaesteker, Dieter Deforce, Filip Van Nieuwerburgh, Pieter Mestdagh, Jo Vandesompele, Bjorn Menten, Katleen De Preter, Lorenz Studer, Bjorn Heindryckx, Kaat Durinck, Stephen Roberts, Frank Speleman
Summary: Human embryonic stem cells and embryonal tumors both exhibit compromised G1/S checkpoint, leading to replicative stress and genomic instability. Long-term in vitro cultured hESCs show a high incidence of DNA copy number gains, which may provide a proliferative advantage.
GENES CHROMOSOMES & CANCER
(2021)
Article
Biochemistry & Molecular Biology
Ioannis I. Moustakas, Angeliki Katsarou, Aigli-Ioanna Legaki, Iryna Pyrina, Konstantinos Ntostoglou, Alkistis-Maria Papatheodoridi, Bettina Gercken, Ioannis S. Pateras, Vassilis G. Gorgoulis, Michael Koutsilieris, Triantafyllos Chavakis, Antonios Chatzigeorgiou
Summary: Senescence is involved in the development of nonalcoholic fatty liver disease (NAFLD) independently of age, with stress-induced senescence playing a role in steatosis development. Hepatocyte senescence was observed in young mice subjected to different diet-induced NAFLD models, with increased expression of senescence-associated genes and presence of senescence-specific staining in the liver. Telomere length and global DNA methylation did not differ between steatotic and control livers, while oxidative stress marker malondialdehyde was upregulated in NAFLD mouse livers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Genetics & Heredity
Lev N. Porokhovnik, Natalia N. Veiko, Elizaveta S. Ershova, Svetlana V. Kostyuk
Summary: This study reviews the role of SatIII in normal stress response, aging, and pathology and proposes a hypothetical universal mechanism as well as a more hypothetical reverse mechanism to explain the balance of SatIII copy numbers and optimum stress resistance. The model is validated on the most recent data on SatIII CNV in pathology and therapy, aging, senescence, and response to genotoxic stress in vitro.
Article
Cell Biology
Miriam Bobadilla Munoz, Josune Orbe, Gloria Abizanda, Florencio J. D. Machado, Amaia Vilas, Asier Ullate-Agote, Leire Extramiana, Arantxa Baraibar Churio, Xabier L. Aranguren, Gloria Cantero, Neira Sainz Amillo, Jose Antonio Rodriguez, Luis Ramos Garcia, Juan Pablo Romero Riojas, Ainara Vallejo-Illarramendi, Carmen Paradas, Adolfo Lopez de Munain, Jose Antonio Paramo, Felipe Prosper, Ana Perez-Ruiz
Summary: Aged muscles have a decline in their response to damage, which is mainly attributed to intrinsic defects in satellite cells. However, changes in the muscle-stem cell microenvironment also contribute to aging. Loss of MMP-10 in young mice alters the muscle ECM and disrupts the satellite cell niche, leading to premature aging features. MMP-10 has a protective effect on satellite cells and can delay their aging and overcome dysfunction in muscular dystrophy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Nutrition & Dietetics
Jieun Woo, Seoungwoo Shin, Hyanggi Ji, Dehun Ryu, Eunae Cho, Youngseok Kim, Junoh Kim, Deokhoon Park, Eunsun Jung
Summary: Research has shown that Isatis tinctoria L. leaf extract (ITE) can prevent and restore skin tissue aging by inhibiting cellular senescence, regulating the MAPK/NF-κB pathway, and suppressing the autophagy pathway.
Review
Health Care Sciences & Services
Lev N. Porokhovnik
Summary: Copy number variation of ribosomal DNA and pericentromeric satellite repeats can modulate the stress response and have phenotypic manifestations, indicating the importance of individual data measurement.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Environmental Sciences
Anwesha Das, Sarbashri Bank, Srilagna Chatterjee, Nirvika Paul, Kunal Sarkar, Arindam Chatterjee, Santanu Chakraborty, Chaitali Banerjee, Anasuya Majumdar, Madhusudan Das, Sudakshina Ghosh
Summary: This study aimed to investigate the effect of the synthetic pyrethroid bifenthrin (BF) on mitochondrial DNA copy number variation in the edible fish Punitus sophore. Results showed that BF reduced the expression level of mitochondria COI gene, inhibited complex IV activity, and led to oxidative stress and mitochondrial dysfunction. BF also induced neurotoxicity by inhibiting AchE activity and caused an imbalance in antioxidant enzyme activity. Molecular docking and simulation analysis predicted that BF binds to the active sites of the enzyme. These findings suggest that the reduction of mtDNAcn could be a potential biomarker to assess BF-induced toxicity in aquatic ecosystems.
Article
Biochemistry & Molecular Biology
Lilas Courtot, Elodie Bournique, Chrystelle Maric, Laure Guitton-Sert, Miguel Madrid-Mencia, Vera Pancaldi, Jean-Charles Cadoret, Jean-Sebastien Hoffmann, Valerie Bergoglio
Summary: This study reveals that low replication stress can lead to advanced DNA replication timing, which is cell-type specific and involves large heterochromatin domains. These advanced events can be inherited by the next generation of cells, leading to changes in chromatin accessibility, replication origin landscape, and gene expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Manuel Hotz, Nathaniel H. Thayer, David G. Hendrickson, Elizabeth L. Schinski, Jun Xu, Daniel E. Gottschling
Summary: This study discovered a previously unappreciated relationship between the number of copies of the ribosomal RNA gene and the replicative lifespan in budding yeast. The data suggest that regulatory mechanisms involving ribosomal DNA copy number limit lifespan and changes in rDNA copy number might explain the effects of lifespan-enhancing mutations.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Hisanori Fukunaga
Summary: Mitochondrial dysfunction is closely related to disease, and environmental factors during fetal life may influence health risks in offspring.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Riva Shmulevich, Valery Krizhanovsky
Summary: Senescence is a crucial biological process that can block tumorigenesis and limit tissue damage, but accumulation of senescent cells can promote age-related diseases. Recent advances have shown the significant role of cellular metabolism in regulating cell senescence, and future research should focus on elucidating metabolic mechanisms to develop new therapeutic strategies.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Article
Food Science & Technology
Fatima Guerrero, Andres Carmona, Maria Jose Jimenez, Teresa Obrero, Victoria Pulido, Juan Antonio Moreno, Sagrario Soriano, Alejandro Martin-Malo, Pedro Aljama
Summary: The study showed that uremic toxins cause accelerated aging in HUVECs, affecting their regenerative capacity and potential to form vascular structures. This phenomenon may contribute to endothelial dysfunction associated with CKD progression.
Article
Clinical Neurology
Katrine M. Johannesen, Yuanyuan Liu, Mahmoud Koko, Cathrine E. Gjerulfsen, Lukas Sonnenberg, Julian Schubert, Christina D. Fenger, Ahmed Eltokhi, Maert Rannap, Nils A. Koch, Stephan Lauxmann, Johanna Krueger, Josua Kegele, Laura Canafoglia, Silvana Franceschetti, Thomas Mayer, Johannes Rebstock, Pia Zacher, Susanne Ruf, Michael Alber, Katalin Sterbova, Petra Lassuthova, Marketa Vlckova, Johannes R. Lemke, Konrad Platzer, Ilona Krey, Constanze Heine, Dagmar Wieczorek, Judith Kroell-Seger, Caroline Lund, Karl Martin Klein, P. Y. Billie Au, Jong M. Rho, Alice W. Ho, Silvia Masnada, Pierangelo Veggiotti, Lucio Giordano, Patrizia Accorsi, Christina E. Hoei-Hansen, Pasquale Striano, Federico Zara, Helene Verhelst, Judith S. Verhoeven, Hilde M. H. Braakman, Bert van der Zwaag, Aster V. E. Harder, Eva Brilstra, Manuela Pendziwiat, Sebastian Lebon, Maria Vaccarezza, Ngoc Minh Le, Jakob Christensen, Sabine Gronborg, Stephen W. Scherer, Jennifer Howe, Walid Fazeli, Katherine B. Howell, Richard Leventer, Chloe Stutterd, Sonja Walsh, Marion Gerard, Benedicte Gerard, Sara Matricardi, Claudia M. Bonardi, Stefano Sartori, Andrea Berger, Dorota Hoffman-Zacharska, Massimo Mastrangelo, Francesca Darra, Arve Vollo, M. Mahdi Motazacker, Phillis Lakeman, Mathilde Nizon, Cornelia Betzler, Cecilia Altuzarra, Roseline Caume, Agathe Roubertie, Philippe Gelisse, Carla Marini, Renzo Guerrini, Frederic Bilan, Daniel Tibussek, Margarete Koch-Hogrebe, M. Scott Perry, Shoji Ichikawa, Elena Dadali, Artem Sharkov, Irina Mishina, Mikhail Abramov, Ilya Kanivets, Sergey Korostelev, Sergey Kutsev, Karen E. Wain, Nancy Eisenhauer, Monisa Wagner, Juliann M. Savatt, Karen Muller-Schluter, Haim Bassan, Artem Borovikov, Marie-Cecile Nassogne, Anne Destree, An-Sofie Schoonjans, Marije Meuwissen, Marga Buzatu, Anna Jansen, Emmanuel Scalais, Siddharth Srivastava, Wen-Hann Tan, Heather E. Olson, Tobias Loddenkemper, Annapurna Poduri, Katherine L. Helbig, Ingo Helbig, Mark P. Fitzgerald, Ethan M. Goldberg, Timo Roser, Ingo Borggraefe, Tobias Brunger, Patrick May, Dennis Lal, Damien Lederer, Guido Rubboli, Henrike O. Heyne, Gaetan Lesca, Ulrike B. S. Hedrich, Jan Benda, Elena Gardella, Holger Lerche, Rikke S. Moller
Summary: This study provides detailed functional analyses and genotype-phenotype correlations in individuals carrying disease-causing variants in SCN8A. The results reveal the clinical phenotypes related to the functional effects and the correlation between the age at seizure onset, type of epilepsy, and the gain- or loss-of-function effects of SCN8A variants.
Article
Biochemistry & Molecular Biology
Svetlana V. Kostyuk, Elena V. Proskurnina, Marina S. Konkova, Margarita S. Abramova, Andrey A. Kalianov, Elizaveta S. Ershova, Vera L. Izhevskaya, Sergey I. Kutsev, Natalia N. Veiko
Summary: This study found that low-dose ionizing radiation and oxidized cell-free DNA can both upregulate mitochondrial gene expression in mesenchymal stem cells, which in turn affects the function of mitochondrial respiratory chain complexes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Pavel E. Umriukhin, Elizaveta S. Ershova, Anton D. Filev, Oksana N. Agafonova, Andrey V. Martynov, Natalia V. Zakharova, Roman V. Veiko, Lev N. Porokhovnik, George P. Kostyuk, Sergey I. Kutsev, Natalia N. Veiko, Svetlana V. Kostyuk
Summary: This study investigated the dynamic changes in repeat sequence contents associated with psychoemotional stress in human leukocytes. The findings showed that psychoemotional stress triggers oxidative stress response and leads to changes in repeat sequence contents. These changes are similar to those observed in patients with schizophrenia during disease exacerbation.
Article
Biochemistry & Molecular Biology
Sofya A. Ionova, Aysylu F. Murtazina, Inna S. Tebieva, Zalina K. Getoeva, Elena L. Dadali, Polina A. Chausova, Olga A. Shchagina, Andrey Marakhonov, Sergey Kutsev, Rena A. Zinchenko
Summary: In this study, three boys with unspecified muscular dystrophy from two unrelated families of Ossetian-Digor origin were described. High-throughput sequencing identified two novel frameshift variants in the COL6A2 gene, one of which was found to be common in the Ossetian-Digor population. The diagnosis of Ullrich muscular dystrophy was made based on molecular genetics, medical history, and clinical examination results.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Elena Zinina, Maria Bulakh, Alena Chukhrova, Oksana Ryzhkova, Peter Sparber, Olga Shchagina, Aleksander Polyakov, Sergey Kutsev
Summary: In Russia, a selective screening program for DMD/BMD has been carried out, which revealed certain differences in the mutation spectrum of the DMD gene among Russian patients. The accurate mutation type plays a crucial role in determining specific therapeutic methods for patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Elena Kondratyeva, Anna Efremova, Yuliya Melyanovskaya, Anna Voronkova, Alexander Polyakov, Nataliya Bulatenko, Tagui Adyan, Viktoriya Sherman, Valeriia Kovalskaia, Nika Petrova, Marina Starinova, Tatiana Bukharova, Sergei Kutsev, Dmitry Goldshtein
Summary: This study evaluated the frequency of the [L467F;F508del] complex allele in Russian cystic fibrosis patients and found that it is present in 8.2% of homozygous F508del patients. Although patients with the F508del/[L467F;F508del] genotype have a severe disease course and lower CFTR channel function, targeted therapy with a combination of ivacaftor + tezacaftor + elexacaftor medication is recommended.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Irina Panchuk, Ekaterina Kondrateva, Anna Demchenko, Olga Grigorieva, Anastasija Erofeeva, Elena Amelina, Vyacheslav Tabakov, Maria Orlova, Ekaterina Voronina, Victoria Pozhitnova, Alexander Lavrov, Svetlana Smirnikhina, Sergey Kutsev
Summary: Induced pluripotent stem cells (iPSCs) were successfully generated from skin fibroblasts obtained from cystic fibrosis patients using the non-integrating, viral CytoTuneT (TM)-iPS 2.0 Sendai Reprogramming Kit, which contains three vectors: polycistronic Klf4-Oct3/4-Sox2, cMyc, and Klf4. The created iPSC lines had a normal karyotype, expressed pluripotency markers, and demonstrated the potential to differentiate into three germ layers in spontaneous differentiation assays.
STEM CELL RESEARCH
(2022)
Review
Genetics & Heredity
Ivan Y. Iourov, Svetlana G. Vorsanova, Oxana S. Kurinnaia, Sergei Kutsev, Yuri B. Yurov
Summary: It is important to recognize the genomic variability of cells in the human brain, as it has been found to play a role in brain diseases. However, brain-specific genetic variations are often overlooked in the study of genetic defects associated with the brain. Therefore, a review of brain-specific somatic mosaicism in the context of disease is needed.
MOLECULAR CYTOGENETICS
(2022)
Article
Genetics & Heredity
Lev N. Porokhovnik, Vladimir M. Pisarev, Anastasia G. Chumachenko, Julia M. Chudakova, Elizaveta S. Ershova, Natalia N. Veiko, Natalia L. Gorbachevskaya, Uliana A. Mamokhina, Alexander B. Sorokin, Anna Ya. Basova, Mikhail S. Lapshin, Vera L. Izhevskaya, Svetlana V. Kostyuk
Summary: This study investigated the relationship between the functional polymorphism of the Nrf2 gene and genotoxic stress responses in ASD children, as well as the effect of a NRF2 inductor. The results showed that adaptive responses to genotoxic stress were positively correlated with NRF2 content in ASD children, particularly in the T+ genotype. ASD children with the NRF2 rs35652124 CC genotype exhibited higher levels of genotoxic stress and NRF2 induction, lower plasma 8-oxo-dG concentration, and higher cfDNA/plasma nuclease activity ratio. These findings suggest that the NEF2L2 rs35652124 polymorphism may impact adaptive responses and potentially be linked to ASD severity.
Meeting Abstract
Biochemistry & Molecular Biology
Sofya Garifullina, Andrey Marakhonov, Vitaly Kadyshev, Tatyana Vasilyeva, Elizaveta Geraskina, Sergey Kutsev, Rena Zinchenko
EUROPEAN JOURNAL OF HUMAN GENETICS
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Vitaly V. Kadyshev, Andrei V. Marakhonov, Inna V. Zolnikova, Sergey I. Kutsev, Rena A. Zinchenko
EUROPEAN JOURNAL OF HUMAN GENETICS
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Elmira P. Adilgereeva, Alex G. Nikitin, Diana G. Zheglo, Oleg A. Shukhov, Svetlana A. Smirnikhina, Alexander V. Lavrov, Ekaterina Y. Chelysheva, Anna G. Turkina, Sergey I. Kutsev
EUROPEAN JOURNAL OF HUMAN GENETICS
(2022)